Objective To discuss effect of serum immunoglobulin M (IgM) and superoxide dismutase (SOD) by percutaneous epidural steroid injections in patients with lumbar disc herniation.Methods 137 patients with lumbar disc herniation were selected in Central of Hospital of Wuhan,all patients were randomly assigned joint group and O3 group, all patients were given ozone (O3) injection treatment, before treatment the joint group was given percutaneous epidural steroid injections, the joint group on the basis of O3 injection method was divided into multi-way group and routine group, followed up for 6 months, analyzed all patients therapeutic effect and recurrence rate,used enzyme-linked immunosorbent method to detect patients with serum IgM, SOD level, Pearson analysis method was used to analyze the relationship between the two groups.Results The joint group treatment effective rate was 91.89%, O3 group treatment effective rate was 79.73%, the former was higher than the latter, the difference was statistically significant ( P<0.05), The joint group serum IgM, SOD level was better than O3 group(P<0.05), In six months, multi-way group recurrence rate was 10.81%, routine group recurrence rate was 18.92%,O3 group recurrence rate was 31.08%, multi-way grou P

Objective To investigate the efficacy of radio-frequency disc nucleoplasty and chemonucleolysis with collagenase for the treatment of lumbar disc herniation.Methods Eighty patients with lumbar disc herniation were treated by nucleoplasty combined with chemonucleolysis using collagenase(1200 U) in our hospital from January 2002 to January 2007.Results The patients were re-examined in 2 weeks,4 weeks and 6 months after the operation.According to the modified MacNab criteria,62 patients achieved excellent outcomes,10 were good,6 were fair,and 2 were poor.The rate of excellent and good outcomes were 90%(72/80).No serious complications occurred in this series.Conclusion Radio-frequency disc nucleoplasty and chemonucleolysis with collagenase are minimally invasive and effective for lumbar disc hernia.

Objective To investigate the relationship of serum soluble CD40L with diabetic retinopathy(DR) in patients with type 2 diabetes mellitus(DM). Methods Serum soluble CD40L level was measured by ELISA in 30 diabetic patients without diabetic retinopathy (NDR), 29 patients with background diabetic retinopathy (NPDR), 31 patients with proliferative diabetic retinopathy (PDR), and 32 normal control(NC) subjects. Results The serum soluble CD40L levels were higher in NDR,NPDR and PDR groups [(4.55±3.66), (6.65±4.24), (8.31±5.23) μg/L,P<0.01] than in NC group[(2.01±1.35)μg/L]. Conclusions Sorluble CD40L level in serum is significantly increased in type 2 DM with diabetic retinopathy,which may be correlated with pathogenesis of DR

Adult ; Female ; Humans ; Rheumatic Heart Disease ; therapy ; Treatment Failure

Objective To investigate the change of serum remnant like particle cholesterol (RLP C) and the association of RLP C with diabetic nephropathy (DN) in the patients with type 2 diabetes mellitus (DM). Methods Serum RLP C concentration was assayed by immunoseparation method. Plasma ? granule membrane glycoprotein 140 (GMP140) and nitric oxide (NO) levels were also measured. Urinary albumin excretion rate (UAER) was measured by radioimmunoassay, and the patients with type 2 DM were assigned to three groups according to UAER 〔35 without DN (D 1 group, UAER200 ?g/min)〕. Results Serum RLP C concentration in 96 patients with type 2 DM was significantly increased compared to that in 44 normal controls (NC) 〔(0.281?0.162)mmol/L vs (0.193?0.125)mmol/L, P

Objective To investigate the effects of DanzhiⅠ on rats acute skin trauma model.Method Perforate back of the rats was made with special puncher to establish acute trauma model.Wound surface was applied with Danzhi Ⅰ and the healing process was observed.Result Danzhi Ⅰ significantly shrinked the rim of raw surface and shorten the time of healing.The number of completely healed rats increased obviously.Conclusion Danzhi Ⅰ can significantly accelerate the healing of acute skin trauma of rats.

Objective To observe the changes of intraocular pressure(IOP)in surgical treatment of Arnold-Chiari malformation.Methods The IOP of 20 patients who underwent surgical treatment of ArnoldChiari malformation were detected by Tono-Pen tonometer at 5 min utes after general anesthesia(T0),5 minutes after prone position(T1),30 minutes after prone position(T2),60 minutes after prone position(T3),prone position before the end of operation(T4),5 minutes after recovery prostration position(Ts)and 30 minutes after prostration position(T6),mean aortic pressure(MAP),heart rate(HR)and partial pressure of carbon dioxide in end tidal(PETCO2)were recorded at the same time.With the prone position,head was fixed bead-holder with skull pin.Results There was no statistical difference in MAP,HR,PEtCO2 at each time point(P＞ 0.05).The IOP significantly increased at T1[(20.9 ±2.5)mm Hg(1 mm Hg =0.133 kPa)],T2[(17.7 ± 1.7)mm Hg],T3[(23.9 ±2.4)mm Hg],T4[(26.3 ± 1.0)mm Hg]compared with IOP at T0 [(9.5 ± 1.5)mm Hg](P ＜ 0.05),there were significant differences between T2 and T3,T3 and T4(P ＜ 0.05).Recovery prostration position,the IOP decreased gradually,IOP at T5[(18.6 ± 1.8)mm Hg]was significantly lower than that at T4(P ＜ 0.05),but T5 was still higher than T0(P ＜ 0.05).The IOP at T6[(10.3 ± 1.7)mm Hg]was no difference compared with T0.Inserting of skull pin might be increase IOP for a moment,the IOP at T; was significantly higher than that at T2(P ＜ 0.05).Conclusion IOP gradually increases as the patients prone position changes in surgical treatment of Arnold-Chiari malformation,and inserting of skull pin maybe increase IOP for a moment.

The diversity in Tibetan kefir grains and dynamics of the microbial community during the fermentation of Tibetan kefir by PCR-DGGE fingerprinting technique were studied.The results showed that bacterial community of Tibetan kefir grains was more complex than that of yeast.The bacteria communities between different Tibetan kefir grains showed 78%～84% similarity, and yeast 80%～92%.Bands B, E and N of DGGE profiles of the microbial community during fermentation of Tibetan kefir were present throughout the fermentation.Analysis of sequence date showed the majority of the DGGE bands of bacteria community corresponded to LAB, and the most intense band (band E) was completely homology to Lactococcus lactis.

Objective To report the clinical effect of free transplanting for soft tissue defects pedieled with high site cutaneous branches of the transverse branch of lateral cirumflex femoral artery.Methods Cu- taneous branches of the descending branch of lateral circumflex femoral artery were found small or abscent in 7 patients.The anterolateral femoral skin flap was pedicled with high site cutaneous branches of the transverse branch to repair the soft tissue defects of the arm,hand,leg and foot,rather than with the descending ones. The size of the flap ranged from 15 cm?6 cm to 28 cm?13 cm,with part muscle valve,iliotibal tract and lat- eral femoral cutaneous nerve.The fractures were performed with internal or external fixation.Results All of the anterolateral femoral skin flap survived well postoperatively in the 7 cases and had good appearance and sensation at one stage.The function of the repaired extremities recovered well.Conclusion The anterolat- eral femoral skin flap pedicled with high site cutaneous branches of the transuvrse branch of lateral circumflex femoral artery has many advantages of good blood supply and large size.The flap was secluding,and can be taken with some muscle and lateral femoral cutaneous nerve.When cutaneous branches of the descending branch of lateral circumflex femoral artery is small or abscent,the anterolateral femoral skin flap with high site cutaneous branches of the transverse branch of lateral circumflex femoral artery is an optimal alternative.

Objective To explore whether there were abnormalities of behavioral tests and CNV-like potential in stressed rats following repeatedly forced swim stress.Methods Forty male rats were randomly divided into 4 groups: the control groups (Control-1 and Control-2) and the stress groups ( Stress-1 and Stress-2).Rats in stress groups were administered to repeatedly forced swim 7 or 14 days respectively.Body weight gain, saccharin preference test and open field test were performed.After being anesthetized with urethane, CNV-like potentials were elicited by condition-test stimulus.Results Results of behavioral tests displayed less body weights (F =253.60, P＜0.001 ) and less saccharine solution intake (F= 13.67, P=0.001 ) in stressed group rats and significant effects of stress on the number of crossing squares, the duration of rearing and the number of grooming in open field test.CNV amplitudes were lower in the stressed rats than those in control (F=21.312, P＜0.01 ).Conclusion This study provides an important evidence of changes of CNV-like potential in depressed rats following repeatedly forced swim stress.Based on this study, ER Ps should be taken into consideration and applied as the useful tools in the research work of depressed animal models.