1.Advance in applied anatomy of structure and thrombosis in the left atrial appendage
Jianling SHI ; Hua ZHONG ; Wu YIN
Journal of Medical Postgraduates 2016;29(10):1107-1110
Left atrial appendage ( LAA) arises from the left atrial free wall. It is the remnant of the original embryonic LA, distinct from the rest of the left atrium. It has a unique anatomical structure, physiological function, hemodynamic characteristics. LAA has complex surrounding structures, great variability, and close relation with thrombosis. This paper reviews the morphological and functional characteristics of LAA. Familiar with adjacent relationship of LAA and correlation between LAA and thrombus formation has important clinical significance that can reduce the risk of postoperative complications in interventional procedures, the incidence of car?dioembolic stroke, the clinical misdiagnosis on LAA thrombosis.
2.Clinical analysis of seven acute phosphine poisoning.
Tao CHEN ; Ran SHI ; Xue-zhong YANG ; Xue-zhong YANG ; Ming-jiang QIAN ; Hua-jun CHEN
Chinese Journal of Industrial Hygiene and Occupational Diseases 2005;23(3):223-225
Acute Disease
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Adult
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Humans
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Male
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Occupational Diseases
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diagnosis
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therapy
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Phosphines
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poisoning
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Poisoning
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diagnosis
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therapy
3.Microbiology Resources in Internet and its Application in Multimedia Teaching Technology
Xiang FANG ; Shi-Qing ZHONG ; Li-Qiong GUO ; Mei-Hua MO ; Qin-Ping ZHONG ;
Microbiology 1992;0(05):-
Some important network addresses of microbiology teaching reso ur ces and two effective searching engines in Internet were provided. How to searc h, download and apply these resources to the teaching practice were discussed in this paper.
4.Effects of CO2 pneumoperitoneum on cell cycle and cell cycle protein of a gastric cancer cell line
Yingxue HAO ; Hua ZHONG ; Peiwu YU ; Chao ZHANG ; Dongzhu ZENG ; Yan SHI ; Yun RAO
Chinese Journal of General Surgery 2010;25(5):389-392
Objective To investigate the effects of different CO2 pneumoperitoneum on cell cycle and cell cycle protein of a gastric cancer cell line. Methods Human gastric cancer cell line MNK-45 were exposed to 0,10,12 and 15 mm Hg CO2 pneumoperitoneum in vitro for 4 hrs. Cell cycle was measured by flowcytometry, the expression of CDK4 ,Cyclin D1、Rb and pRb was studied by Western-blot, and the binding ability of CDK4 and Cyclin D1 was evaluated by immunoprecipitation. Results The cell proliferation index in 15 mm Hg CO2 pneumoperitoneum group dropped significantly (27.4% ± 3. 7%) vs. (36. 4% ±3. 3%) ,P <0. 05, while that in other groups did not change significantly. The protein of CDK4、Cyclin D1and binding ability of Cyclin D1 and CDK4 dropped dramatically in the 15 mm Hg CO2 pneumoperitoneum group (0.71%±0.12%),(0.93% ±0.21%),(0.54%±0.11%),(0.18% ±0.02%) vs. (1.05% ±0.16%),(1.40% ±0.24%),(0.75% ±0.14%),(0.31% ±0.02%), all P<0.05. There were no changes of Rb in protein levels, while the phosphorylated Rb dropped obviously. Conclusion There was no obvious effects of clinical CO2 pneumoperitoneum on gastric cancer cells growth and proliferation.
5.Effects of HIF-1α on human gastric cancer cell's apoptosis in CO2 pneumoperitoneum environment
Yingxue HAO ; Hua ZHONG ; Dongzhu ZENG ; Yan SHI ; Yun RAO ; Lixin ZHOU ; Peiwu YU
Chinese Journal of General Surgery 2008;23(6):460-463
Objective To study the effect and mechanism of human gastric cancer cell's apoptosis in simulated CO2 pneumoperitoneum environment by HIF-1α. Methods We used a closed box to simulate CO2 pneumoperitoneum environment, a standard surgical insufflator maintained pressure of the box at 0,5,10 and 15 mm Hg respectively. MKN-45 cell's HIF-1α mRNA and protein expression were observed before and after silencing cell's HIF-1α by real time RT-PCR and Western blot. Cell's bcl-2/bax was studied by immunohistochemistry before and after silencing HIF-1α. Cell's apoptosis ratio was measured using Annexin V-FITC/PI double labelled staining. Results In 15 mm Hg group, MKN-45 cell's HIF-1α mRNA and protein expression were significantly higher than control group (P<0.01 ), while the difference was not significant among 0,5,10 mm Hg group and control group (P>0.05). In 15 mm Hg CO2 pressure, cell's bcl-2/bax was obviously lower than in control group (P<0.05) and apoptosis ratio was more than control group(P<0.01). When HIF-1α was silenced, cell's bel-2/bax and apoptosis ratio weren't significantly different between 15 mm Hg group and control group (P>0.05 ). Conclusions MKN-45 cell's apoptosis did not experience any alterations under 0,5,10 mm Hg.15 mm Hg CO2 pneumoperitoneum environment enhanced cell's apoptosis probably by way of HIF-1α.
6.Composite glandular-neuroendocrine carcinoma in gastric cardia: report of a case.
Zhang-lei ZHOU ; Xin-hua ZHANG ; Hang-bo ZHOU ; Zhong-qiu WANG ; Qun-li SHI
Chinese Journal of Pathology 2009;38(11):779-780
Adenocarcinoma
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metabolism
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pathology
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surgery
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ultrastructure
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Aged
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Carcinoembryonic Antigen
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metabolism
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Carcinoma, Neuroendocrine
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metabolism
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pathology
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surgery
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ultrastructure
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Cardia
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Humans
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Ki-67 Antigen
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metabolism
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Male
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Microscopy, Electron, Transmission
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Stomach Neoplasms
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metabolism
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pathology
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surgery
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ultrastructure
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Synaptophysin
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metabolism
7.Study on effect of total flavonoids of Oldenlendia difflusa on ulcerative colitis and its immunological mechanism.
Shi-Ying LUO ; Zhou LE ; Xiao-Hua LV ; Zhi-Guo ZHONG
China Journal of Chinese Materia Medica 2014;39(5):896-900
OBJECTIVETo observe the effect of total flavonoids of Oldenlendia difflusa (FOD) on NF-kappaB and IL-8, TNF-alpha, IL-10 expressions of ulcerative colitis (UC) model rats, and explore its immunological mechanism of anti-UC.
METHODSixty Kunming male mice with the average weight of (20 +/- 2) g were randomly divided into six groups. The control group (cont) was orally administered with distilled water. Whereas the remaining five groups were fed with 4% dextran sulphate sodium (DSS) solution for seven days to induce acute UC, and orally administered with the following drugs: distilled water (for the DSS group), SASP at dose of 500 mg x kg(-1) x d(-1) for the DSS + SASP group, FOD at dose of 60 mg x kg(-1) x d(-1) for the DSS + FOD-H group, FOD at dose of 40 mg x kg(-1) x d(-1) for the DSS + FOD-M group, and FOD at dose of 26.7 mg x kg(-1) x d(-1) for the DSS + FOD-L group. During the modeling and drug administration, the mice were scored for DAI. Seven days later, the mice were put to death, and their colonic tissue samples were collected to evaluate colonic mucosal lesions. The NF-kappaB p65, IL-8, TNF-alpha, IL-10 expressions were tested by immunohistochemical staining and ELISA.
RESULTSeven-day feeding with 4% DSS solution could successfully induce acute UC in mice. Compared with the cont group, the DSS group showed significantly higher DAI and colonic mucosal lesions, remarkable increase in NF-kappaB p65, IL-8, TNF-alpha expression in colonic tissues, and notable decrease in IL-10 expression (P < 0.05). FOD could prevent acute UC in mice included by DSS. Seven-day administration of 60 mg x kg(-1) x d(-1) or 40 mg x kg(-1) x d(-1) FOD could completely or partially resist the above mentioned changes caused by DSS. Compared with the DSS group, the DSS + FOD-H group and the DSS + FOD-M group showed reduction in colonic mucosal lesions, down-regulation in IL-8, TNF-alpha and NF-kappaB p65 expressions and up-regulation in IL-10 expression (P < 0.05).
CONCLUSIONFOD could significantly resist UC in mice. Its mechanism may be related to the inhibition of NF-kappaB p65 activation, the reduction of IL-8 and TNF-alpha expressions and the increase in the anti-inflammatory factor IL-10.
Animals ; Anti-Inflammatory Agents ; administration & dosage ; Colitis, Ulcerative ; drug therapy ; genetics ; immunology ; Drugs, Chinese Herbal ; administration & dosage ; Flavonoids ; administration & dosage ; Humans ; Interleukin-8 ; genetics ; immunology ; Male ; Mice ; NF-kappa B ; genetics ; immunology ; Oldenlandia ; chemistry ; Transcription Factor RelA ; genetics ; immunology ; Tumor Necrosis Factor-alpha ; genetics ; immunology
8.Diagnosis realization of knee joint synovial chondromatosis:clinical data of 28 cases.
Qin-Zhong ZHANG ; Shi-Liang WU ; Shuang-Ming FEI ; Hua CAO ; Wu-Lin QI
China Journal of Orthopaedics and Traumatology 2012;25(8):694-696
OBJECTIVETo investigate diagnosis and therapeutic effects of knee joint synovial chondromatosis with arthroscopic.
METHODSFrom March 1995 to July 2011, 28 patients with knee joint synovial chondromatosis were treated. Among them, 18 males and 10 females ranging age from 25 to 81 (mean 55.2) years,the course of disease ranged from 0.5 to 15 (mean 5.6) years. Clinical manifestation mainly included pain, swell and functional limitation of knee joint. Knee open surgery (17 cases) and laparoscopic surgery (10 cases) were respectively used. Clinical symptom,image data,pathological manifestation and effects under arthroscopy were observed, Lysholm scoring was used to evaluate effects.
RESULTSAll patients were followed up except one lost, the duration ranging from 6 to 24 months. Lysholm score in knee open surgery was increased from (41.89 +/- 6.81) preoperatively to (67.73 +/- 7.62) postoperatively;while in laparoscopic surgery it was increased from (40.78 +/- 7.54) preoperatively to (77.46 +/- 8.43) postoperatively.
CONCLUSIONArthroscopic surgery, which has no risk of rupture of incision, nonunion, earlier to exercise, is a good method to diagnosis and treat knee joint synovial chondromatosis.
Adult ; Aged ; Aged, 80 and over ; Chondromatosis, Synovial ; diagnosis ; surgery ; Female ; Follow-Up Studies ; Humans ; Knee Joint ; surgery ; Laparoscopy ; Male ; Middle Aged ; Treatment Outcome
9.Construction and thinking of data element standard directory of traditional Chinese medicine clinical pharmacy information.
Xiao-Xia WANG ; Zhong-Zheng JIN ; Gui-Ming GUO ; Hua-Qiang ZHAI ; Shi-Yuan JIN
China Journal of Chinese Materia Medica 2014;39(9):1724-1727
The aim of this study was to develop the data element standard directory of traditional Chinese medicine (TCM) clinical pharmacy information, to provide application standards and models of TCM clinical pharmacy for the electronic medical record (EMR). The developed line of work is as follows: initially establish research through four forms: literature analysis, questionnaires, discussion groups, expert advice. The research range from the Chinese herbal medicine research, herbal origin, harvesting, processing, identification of traits, physical and chemical identification, modern research, character, taste, Indications, clinical application, processing, dispensing medicine, Chinese medicine specifications, usage, dosage, caution, efficacy indications to small packaging applications, drug research, management and other related issues, including traditional Chinese medicine theory, application and hospital management information; according to the general and part 16 content of the national "Health Information Data Element Standards", and the basic method of extracting data element to study and develop the data element of TCM clinical pharmacy information from the defining content. Correspondingly propose the ideas and methods of construction of the "Data Element Standard Directory of TCM Clinical Pharmacy Information", sort out medicine clinical information data element standard catalog, divided into basic categories, clinical application class, management class three parts, and set norms and standards of identifying data elements, definitions, allowable value of traditional Chinese medicine clinical information, and discuss the sources and standards of information collection, leaving the interface, standardized and scientific terminology, docking with the existing standards, maintenance and management program and oter issues.
China
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Data Mining
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methods
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statistics & numerical data
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Database Management Systems
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standards
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statistics & numerical data
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Electronic Health Records
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standards
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statistics & numerical data
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Evidence-Based Medicine
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methods
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statistics & numerical data
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Humans
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Information Dissemination
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methods
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Medicine, Chinese Traditional
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methods
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Phytotherapy
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methods
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statistics & numerical data
10.Application of CT in assessment of displaced intraarticular calcaneal fractures
Zhi-Jie WANG ; Shi-Zhen ZHONG ; Ji-Hua LIU ; Zi-Hai DING ;
Chinese Journal of Radiology 2000;0(12):-
Objective To evaluate the significance of CT assessment for extraarticular anatomy in treatment of displaced intraarticular calcaneal fractures.Methods(1)Measurement of normal calcaneum 40 pieces of adult calcaneum specimen were measured,items of measurement included height of culmination of posterior facet and tuberosity,width of posterior edge of sustentaculum and tuberosity.(2)CT measurement of calcaneum.Transverse(axial)and coronal CT scanning were obtained from 20 feet with displaced intraarticular calcaneal and 20 normal feet as control.Following items were measured in CT scanning:the height of culmination of posterior facet and tuberosity,the coronal talocalcaneal angle,in coronal scanning,the width of posterior edge of sustentaculum and tuberosity,the axial calcaneocuboid angle,in axial scanning.Results(1)Measurement of height of calcaneum height of culmination of posterior facet and tuberosity of calcaneal specimen were(43.07?2.85)mm and(44.69?3.67)mm respectively,and these two items from CT scanning of normal feet were(42.84?1.66)mm,(43.40? 3.01)nun,and from CT scanning of feet with calcaneal fractures were(34.76?3.24)mm,(40.41? 3.69)mm.There was a statistically significant different between these two items for normal calcaneal specimen and for CT scanning of feet with ealcaneal fractures(P