OBJECTIVE To investigate the prevention from hospital infection and therapy in fracture patients.METHODS We analyzed the situation of hospital infection in the fracture patients ranged from 2000 to 2005 with reviewing and perspective statistical method.RESULTS Totally 43 cases were infected from 1345 fracture patients,taken 3.2%.It was the highest with infection rate under the age of 5(6.0%),then was successively at the 15-44 years old age,5-14 years old age,≥60 and 45-59 years old age.CONCLUSIONS The fracture patients or those experiencing surgical operation are easy to have hospital infection.It is a multifactorial result.For instance,the environment of ward,the disinfection measure in operation,the skilled degree of surgical operators,the length of operation time,rational selection antibiotics and so on,are all the important factors to prevent and cure hospital infection.Pay attention to the quality controlling every link,can reduce the rate of hospital infection among fracture patients.

Objective Apuinic/apyrimidic endonuclease/redox effector factor-1(APE1/Ref-1,abbreviated as APE1) is a major member of the base excision repair(BER) pathway involved in oxidative DNA damage repair.To knock down APE1 gene expression in HOS cells with pSilence APE1,and explore its effect in combination with 252Cf neutron ray radiotherapy.Methods The constructed APE1 siRNA expression vector pSilence APE1 was transfected into HOS cells by SuperFect Transfection liposome,and it was used to knock down the expression of APE1.The HOS cells and transfected HOS cells were respectively irradiated by 252Cf neutron ray,then the cell survival(D0),DNA single strand breaks(SSB) and cell apoptosis were determined by clone formation assay,alkaline comet assay and flow cytometer.Results The cell-survival curve was plotted by clone formation assay,the D0 value was 2.80 vs.1.89 and Dq value was 2.66 vs.2.00 for the control and transfected HOS cells,respectively,after being irradiated by 252Cf neutron ray.The tail moments at 2,5 and 10 Gy were 6.664?0.648 vs.7.997?0.542,20.322?1.433 vs.25.238?1.185 and 33.909?1.245 vs.39.191?1.052,respectively,for the control and transfected HOS cells,and the cell apoptosis rate at 2,5 and 10 Gy was 4.00 vs.5.68,5.91 vs.7.55 and 9.63 vs.13.51,respectively,for the control and transfected HOS cells.All these findings showed significant difference between the two groups(P

Objective To find out a new teaching pattern of Medical Statistics for medical postgraduate students.Methods To manifest the longitudinal thought of the medical research process from the teaching idea,the teaching content arrangement and the teaching method utilization.Results To discuss the superiority of the new pattern which provides the content frame and the teaching method based on the integrating theory and the practice.Conclusion The teaching reformation of Medical Statistics must be advantageous in improving the learner’s power of the medical scientific research,including selection of the scientific research topic,application of project,the quality control of the project,analysis of the material and inference of the conclusion,paper writing,self-appraisement and achievement evaluation.

Objective: To evaluate the protective effect of epidermal growth factor (EGF) on intestinal barrier functionin rats with acute pancreatitis. Methods: Thirty-two male SD rats received injection of sodium taurocholate solution(3. 5 mg?L-1) into the pancreatic duct were randomly divided into control group (n=16) and treatment group (n=16). Animals incontrol group received total parenteral nutrition (TPN), animals in treatment group were fed on the same TPN formula ascontrol group and injections of EGF at a dose of 0. 2 mg' kg l' day--'. Rats were sacrificed on d 1 and d 5 of TPN. Concen-tration of xylose and fluorescein isothiocyanate (FITC)-dextran in superior mesenteric vein (SMV), protein and DNA contentin je junal mucosa were determined. Samples from SMV, mesenteric lymph nodes, pancreas, liver, spleen were harvested forcultures. Results: FITC-dextran concentration in treatment group was significantly lower than in control group [(3. 4?0. 7)vs (7. 5?0. 9) mg. L-1, P＜0. 0l]. Protein and DNA content in je junal mucosa in treatment group were significantly higherthan in control group [(2. 65?0. 23) vs (1. 12?0. 18) mg? cm-1, (0. 25?0. 07) vs (0. 12?0. 04) mg?cm-1, P

Objective To investigate the role of survivin and P53 in apoptosis of gastric adeno- carcinoma,as well as its relationship with the clinicopathologic features and the prognosis.Methods The gas- tric tissue microarrays were composed of those from 100 cases of gastric cancer and 30 controls.At these tissue microarrays,expressions of survivin and P53 were investigated immunohistochemically,and tumor cell apoptosis index was examined by TUNEL method.Of the 100 cases,47 cases were followed-up from 14 months to 13 years,in which the survival was analyzed.Results Two paraffin-embedded gastric carcinoma tissue micro- arrays were successfully constructed,including 114 and 116 tissue spots,respectively.Immunohistochemical analysis showed that survivin was expressed in 78 cases (78%).No expression of survivin was detected in control tissue (P0.05).In the 47 cases with followed-up data,univariant analysis revealed that the survival was correlated with invading of vessel and nerve,TNM stages,and expression of survivin.The histological grades and expression of P53 were not related to prognosis.However,Cox stepwise proportional hazards analysis showed that only TNM staging and survivin status retained significant independently in prospecting prognosis.Conclusions The expression of survivin was associated with the pathologic features,TNM stages and prognosis in gastric carcinoma,indicating that overex- pression of survivin may be a poor prognosis factor for gastric carcinoma.

0.05),while the changes in the serum levels of AST and ALT in the recipients in of treatment group were obviously lower(P

Objective To estimate the role of the posterior corneal parameters measured by Pentacam in screening subclinical keratoconus.Design Retrospective case-controlled study.Participants Forty five healthy subjects(73 eyes),14 keratoconus patients (20 eyes),43 subclinical keratoconus patients(58 eyes).Methods Based TOPOLYZER and biomicroscope signs,eyes were diagnosed as subclinical keratoconus and keratoconus to compare the differnces of related posterior corneal parameters provided by Pentacam in normal eyes and subclinical keratoconus or keratoconus eyes.Main Outcome Measures Back-Diff(6 mm) max,Back-Diff(6 mm )min, Back-Difference(6 mm),Back-Rmin,Back-Astig.Results The parameters which were statistically different between normal corneas and clinical keratoconus,normal corneas and subclinical keratoconus include Back-Diff(6 mm)max(P

Objective: To determine the inhibitory effects of maltitol-gum on Streptococcus mutans, Lactobacilli and Actinomyces viscosus in dental plaque. Methods: Thirty 13-15 years old children with DMFS>4 were divided into three groups, maltitol chewing gums group(A group), xylitol chewing gum group(B group) and blank gum base group (C group). The plaque samples were collected and colony forming units were counted. Results: The levels of three-species cariogenic pathogens in three groups were statistically down-regulated when compared with the baseline(P<0.001).Moreover, A group and B group resulted in a higher decrease of Streptococcus mutans levels compare with C group(P<0.05). The levels of Lactobacilli and Actinomyces viscosus were not statistically different between groups(P>0.05). Conclusion: Maltitol-gum can lead to a significant suppression on Streptococcus mutans levels in dental plaque,while the inhibitory effect of the maltitol-gum on Lactobacilli, Actinomyces viscosus is not obvious.

Aconitum ; poisoning ; Animals ; Disease Models, Animal ; Female ; Hemoperfusion ; methods ; Male ; Plant Poisoning ; therapy ; Rabbits

Objective To determine the inhibitory effect of APE1 siRNA expression vector pSilence APE1, and the possible synergetic role of pSilence APE1 and endostatin on endothelial cell migration induced by osteosarcoma cell 9901 and HOS . Methods The osteosarcoma cells and endothelial cells were co-cultured with transwell model, and the cell number through the inner membrane was counted to evaluate the inhibitory effect of endothelial cell migration by pSilence APE1 and its combination with endostatin. Results Both low dose (350 ng/ml) and high dose (700 ng/ml) of endostatin showed significant inhibition to endothelial cell migration induced by osteosarcoma cell, while the high dose showed much stronger inhibition than the low dose (P