Seventy C57BL/6 mice were divided into control group,lard group,lard transferred into safflower oil group,and safflower oil group.After 20 weeks,the white adipose tissues were taken to analyse the gene differentiational expression prolife.The results showed that body weight,blood glucose and lipids,and insulin levels in lard group were higher than those in control group( all P＜0.05 ),which were decreased after lard was transferred into safflower oil for 10 weeks( all P＜0.05 ).Safflower oil regulated some of the orexigenic genes,anorectic genes,and the genes involved in energy expenditure in adipose tissues such as opioid receptor,glucagon,and PPARα.

This paper reviews the common approaches for B cell epitopes and T cell epitopes study in recent years, and its application in foot-and-mouth disease virus (FMDV)’s antigen epitope study. The development of antigen epitopes of FMDV are also summarized.

Objective To compare the difference of serum heme oxygenase-1 (HO-1) and carbon monoxide(CO) levels between the patients with coronary heart disease(CHD) caused chronic heart failure(CHF) and CHD patients with normal cardiac function,and further to explore the protective mechanism of HO-1/CO system during the pathogenesis process of CHF.Methods Ninety-one patients with CHF were selected as the observation group and 72 CHD cases with normal cardiac function were taken as the control group.The concentration of HO-1 was determined by ELISA arid the Chalmer S method was used to detect serum CO concentration.The general clinical data of the two groups were recorded by the using the heart failure questionnaire.And the liver and kidney functions,blood lipids,NT-proBNP,BNP and cardiac echocardiography examination were performed.Results The serum HO-1 level in the observation group was (8.13±0.27)ng/mL,which was higher than (2.80±0.52)ng/mL in the control group;the CO level in the observation group was (0.35±0.06)mg/L,which was lower than(0.59±0.07)mg/L in the control group,the difference was statistically significant(P＜0.01);the HO-1 level in the observation group was gradually increased with the increase of cardiac function grade (P＜0.01);while the CO level was decreased with the increase of cardiac function grade (P＜0.01).Conclusion The serum HO-11evel in the patients with CHF is highly expressed with the heart failure aggravation;endogenous CO is gradually decreased due to consumption after cardiac failure aggravation.

In recent years,using stem cells to repair the bladder defect is a hot issue on the studies of bladder tissue engineering.They are ideal seed cells for bladder regeneration of tissue engineering depending on their self-renewal and multi-differentiation potential.In this paper,we give a review on the use of embryonic stem cells and adult stem cells as seed cells to repair the bladder defect by tissue engineering approach.

Objective To discuss the curative effect and functionary mechanism of intraparotid injection on Sjogren’s syndrome (SS), validate the action of restoring the parotid configuration destroyed by immunoreaction and protecting unspoiled parotid, testify the action of improving the function of parotid excreting saliva by intraparotid injection. Methods Patients diagnosed as SS were divided into control group and treatment group. The patient of two groups were all treated by the same dosage hormone and Runzaoling capsule, at the same time, the treatment group were added the intraparotid injection. The parotid configuration, morphologic manifestation and functional melioration were observed. Result There was remarkable difference about the change of parotid configuration (t=1.67, P

Objective To investigate the relationship between diagnosis and treatment of the primary peritoneal carcinoma(PPC)and its prognosis.Methods The clinical materials of 10 women,who were diagnosed with PPC,were analyzed retrospectively from Mar.2000 to Nov.2006.Results We found that PPC has lower rate of incidence,higher rate of wrong diagnosis,wider spread in pelvis cavity and abdominal cavity and had no significant clinical signs and symptoms specificity.All of the patients needed cytoreductive surgery,but the majority of rudimental focus diameter is larger than 2 cm.After the operation,platinum combined other chemotherapeutic drug were needed.Conclusion Abdominal distention,ascites,abdominal pain are the main symptoms and signs of PPC.we need choose cytoreductive surgery as much as possible.Extra trauma to patients will affect restoration and survival time of patients and the effect of chemotherapy.We also found the effect of abdominal cavity chemotherapy may be better than that of chemotherapy in vein.

ObjectiveTo selected the safe dosage of intrathecal injection of sufentanil for preemptive analgesia in lower limb orthopaedic surgery.MethodsSelected 96 patients who ASA classification Ⅰ - Ⅱ grades undergoing elective lower limb orthopaedic surgery with subarachnoid epidural block down,they were divided into six groups by random number table and double blind method,each group of 16 cases.Group Ⅰ,Ⅱ,Ⅲ,Ⅳ,Ⅴ,Ⅵ received 0.05,0.10,0.15,0.20,0.25,0 μ g/kg of intrathecal sufentanil and 2.5 ml of 0.5％ levobupivacaine.Observed and recorded the duration of block level of pain to T10,visual analog score (VAS)postoperative at 2,4,6,8,12,24,48 h,and side effects.ResultsThe duration of block level of pain to T10 of group Ⅰ,Ⅱ,Ⅲ,Ⅳ,Ⅴ,Ⅵ was (245.6 ± 34.2),(259.3 ± 36.7),(268.9 ± 37.8),(286.6 ± 38.8),(287.7 ± 39.1 ),(228.6 ± 31.4) min.There was no significant difference between group Ⅳ and group Ⅴ (P ＞ 0.05 ),the differences of other groups were statistically significant (P ＜ 0.05 ).Compared with group Ⅴ,VAS of group Ⅲ and group Ⅳ at 6,8,12,24,48 h after operation was not statistically significant (P ＞ 0.05),and they were lower than the other three groups(P ＜ 0.05 ).There were no respiratory depression occurred after operation in group Ⅰ,Ⅱ,Ⅲ,Ⅳ,Ⅵ,4 cases occurred in group Ⅴ.ConclusionThe best of effective dose of intrathecal injection of sufentanil for preemptive analgesia in lower limb orthopaedic surgery is 0.20 μ g/kg.

0.05 ).Side effects of group A,17%(2/14),were lower than that of group B or C,53%(8/15)and 47%(7/15),respectively(? 2 test=12.21, P

Objective:To detect the expression of cyclin B 1 in human ameloblastoma(AB). Methods:Expression of cyclin B1 was detected in 73 cases of AB (32 primary, 33 recurrent and 8 malignant cases), 19 of odontogenic keratocyst (OKC) and 7 of normal oral mucosa by streptovidin-biotin method. Results:The ratio of positive nuclear expression of cyclin B 1 in normal oral mucosa, OKC and AB was 28.6%(2/7), 36.8%(7/19) and 63.0%(46/73) respectively (P

Objective:To study the correlation between the degree of rh eumatoid arthritis(RA) and periodontal bone loss. Methods:70 cas es of RA were included. Periodontal bone loss was examined by clinical attachmen t loss(CAL) test and radiography. The degree of RA was determined by the measure ments of morning stiffness time (MST),erythrocyte sedimentation rate(ESR)and C -reactive protein(CRP).Results:MST,ESR and CRP were positivel y related to the levels of bone loss(P