Neutrophil extracellular traps (NETs) can capture pathogenic microorganisms and inhibit them spreading,kill and eliminate pathogens,and participate in occurrence and development of autoimmune diseases.The steriliza-tion way of neutrophil different from chemotaxis and phagocytosis becomes a study hotspot in recent years.NETs not only appear in thrombus and plaques,they may also play an important role in formation of atherosclerotic plaques and arterial thrombus.The present article made a review on research progress of NETs participation in for-mation of atherosclerotic plaques and thrombosis.

BACKGROUND AND OBJECTIVELittle is known about the incidence of hepatitis B virus (HBV) infection in Hodgkin's lymphoma patients. This study was to evaluate the impact of HBV infection on the survival of Hodgkin's lymphoma patient.

METHODSClinical data of 120 Hodgkin's lymphoma patients treated at the Sun Yat-sen University Cancer Center between January 2004 and October 2007 were collected. The impact of prognostic factors including HBV infection on survival was examined by univariate and multivariate analyses. A log-rank test was used for univariate analysis and the Cox proportional hazards regression model was used for multivariate analysis.

RESULTSOf the 120 patients, 18 (15.0%) were hepatitis B virus surface antigen HBsAg-positive. The HBsAg-positive patients had lower 5-year survival rate than did the HBsAg-negative ones (66.9% vs. 91.3%, P = 0.006). When the patients were divided into early-stage (I + II) and advanced-stage (III + IV) groups, the 5-year survival rate was significantly different between the HBsAg-positive and -negative patients in early-stage group (64.8% vs. 96.0%, P < 0.001), while not significantly different in advanced-stage group (75.0% vs. 84.8%, P = 0.667). Both univariate and multivariate analyses showed that radiotherapy and HBV infection were independent prognosis factors for the patients with early-stage Hodgkin's lymphoma (P = 0.006 and 0.014, respectively).

CONCLUSIONSThe incidence of HBV infection is similar between Hodgkin's lymphoma patients and normal population. HBV infection is an independent prognosis factor for survival in the patients with early-stage Hodgkin's lymphoma.

Adult ; Antineoplastic Combined Chemotherapy Protocols ; therapeutic use ; Disease-Free Survival ; Female ; Hepatitis B ; blood ; Hepatitis B Surface Antigens ; blood ; Hodgkin Disease ; drug therapy ; radiotherapy ; virology ; Humans ; Male ; Neoplasm Staging ; Proportional Hazards Models ; Survival Rate

Background and Objective:Little is known about the incidence of hepatitis B virus(HBV)infection in Hodgkin's lymphoma patients.This study was to evaluate the impact of HBV infection on the survival of Hodgkin's lymphoma patient.Methods:Clinical data of 120 Hodgkin's lymphoma patients treated at the Sun Yat-sen University Cancer Center between January 2004 and October 2007 were collected.The impact of prognostic factors including HBV infection on survival was examined by univadate and multivariate analyses.A log-rank test was used for univadate analysis and the Cox proportional hazards regression model was used for multivadate analysis.Results:Of the 120 patients,18(15.0%)were hepatitis B virus surface antigen(HBsAg)-positive.The HBsAg-positive patients had lower 5-year survival rate than did the HBsAg-negative ones(66.9% vs.91.3%,P=0.006).When the patients were divided into early-stage(Ⅰ+Ⅱ)and advanced-stage(Ⅲ+Ⅳ)groups,the 5-year survival rate was significantly different between the HBsAg-positive and-negative patients in early-stage group(64.8% vs.96.0%,P＜0.001),while not significantly different in advanced-stage group(75.0% vs.64.8%,P=0.667).Both univariate and multivariate analyses showed that radiotherapy and HBV infection were independent prognosis factors for the patients with early-stage Hodgkin's lymphoma (P=0.006 and 0.014,respectively).Conclusions:The incidence of HBV infection is similar between Hodgkin's lymphoma patients and normal population.HBV infection is an independent prognosis factor for survival in the patients with early-stage Hodgkin's lymphoma.

Betacoronavirus ; Clinical Laboratory Techniques ; Coronavirus Infections ; diagnosis ; False Negative Reactions ; Humans ; Nucleic Acid Amplification Techniques ; Pandemics ; Pneumonia, Viral ; diagnosis

Objective To identify the pathogenic variant responsible for restrictive cardiomyopathy (RCM) in a Chinese family.Methods Next generation sequencing was used for detecting the mutation and Results verified by sequencing. We used restriction enzyme digestion to test the mutation in the family members and 200 unrelated normal subjects without any cardiac inherited diseases when the mutation was identified.Results Five individuals died from cardiac diseases, two of whom suffered from sudden cardiac death. Two individuals have suffered from chronic cardiac disorders. Mutation analysis revealed a novel missense mutation in exon 7 of troponin I type 3 (TNNI3), resulting in substitution of serine (S) with proline (P) at amino acid position 150, which cosegregated with the disease in the family, which is predicted to be probably damaging using PolyPhen-2. The mutation was not detected in the 200 unrelated subjects we tested.Conclusion Using next generation sequencing, which has very recently been shown to be successful in identifying novel causative mutations of rare Mendelian disorders, we found a novel mutation of TNNI3 in a Chinese family with RCM.