1. The cleavage of pyrophosphate by human serum apo-transferrin
Academic Journal of Second Military Medical University 2006;27(1):71-75
Objective: To determine the rate constants for the cleavage of pyrophosphate to phosphate by human serum apotransferrin. Methods: The cleavage was followed by31P NMR spectroscopy. The data for concentration changes of pyrophosphate (as determined from its NMR peak intensity) with time, in the absence and the presence of MgCl2, at different pH values, were fitted to give second order rate constants. Results: The rate constnts at 312 K were 8.83×10-4 L · mmol-1 · h-1 at pH 6.85, 9.59×10-4 L · mmol-1 · h-1 at pH 7.4, and 1.38×10-3 L · mmol-1 · h-1 at pH 8.15, for reactions of apo-transferrin (0.5-1.0 mmol/L) with 5 molar equivalence of pyrophosphate. The rate constant increased to 1.21×10-3 L · mmol-1 · h-1 at pH 7.4,312 K when 2 mmol/L MgCl2 was added. Conclusion: The cleavage of pyrophosphate to phosphate by human serum apotransferrin is very slow and follows the second-order kinetics. Mg2+ can slightly enhance the rate of the cleavage.
2.Investigation on the pneumosilicosis in small cast steel factories.
Zhong-Qing WU ; Jing-Xia PU ; Wei ZHU
Chinese Journal of Industrial Hygiene and Occupational Diseases 2004;22(4):273-274
Adult
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China
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epidemiology
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Dust
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Humans
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Iron
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Male
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Metallurgy
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Occupational Exposure
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Pneumoconiosis
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diagnostic imaging
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epidemiology
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prevention & control
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Radiography
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Risk Factors
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Steel
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Welding
3.Protective effect and mechanism of sodium tanshinone II A sulfonate on microcirculatory disturbance of small intestine in rats with sepsis.
Wei, ZHU ; Qing, LV ; Huawen, CHEN ; Zhaohua, WANG ; Qiang, ZHONG
Journal of Huazhong University of Science and Technology (Medical Sciences) 2011;31(4):441-5
To explore the protective effect of sodium tanshinone IIA sulfonate (STS) on microcirculatory disturbance of small intestine in rats with sepsis, and the possible mechanism, a rat model of sepsis was induced by cecal ligation and puncture (CLP). Rats were randomly divided into 3 groups: sham operated group (S), sepsis group (CLP) and STS treatment group (STS). STS (1 mg/kg) was slowly injected through the right external jugular vein after CLP. The histopathologic changes in the intestinal tissue and changes of mesenteric microcirculation were observed. The levels of tumor necrosis factor-α (TNF-α) in the intestinal tissue were determined by using enzyme-linked immunoabsorbent assay (ELISA). The expression of intercellular adhesion molecule-1 (ICAM-1) in the intestinal tissue was detected by using immunohistochemisty and Western blot, that of nuclear factor κB (NF-κB) and tissue factor (TF) by using Western blot, and the levels of NF-κB mRNA expression by using RT-PCR respectively. The microcirculatory disturbance of the intestine was aggravated after CLP. The injury of the intestinal tissues was obviously aggravated in CLP group as compared with S group. The expression levels of NF-κB p65, ICAM-1, TF and TNF-α were upregulaed after CLP (P<0.01). STS post-treatment could ameliorate the microcirculatory disturbance, attenuate the injury of the intestinal tissues induced by CLP, and decrease the levels of NF-κB, ICAM-1, TF and TNF-α (P<0.01). It is suggested that STS can ameliorate the microcirculatory disturbance of the small intestine in rats with sepsis, and the mechanism may be associated with the inhibition of inflammatory responses and amelioration of coagulation abnormality.
4.Quantitative detection of the expression level of transform-ing growth factor-β and its receptors in pterygium with RT- PCR
Ming, ZHONG ; Wei, SHEN ; Qing, FU ; Yuan, ZHANG
International Eye Science 2009;09(4):619-622
AIM: To quantitatively investigate transforming growth factor-β(TGF-β) and its receptors in normal bulbar conjunctival tissues and pterygium tissues. METHODS: Thirty cases of pterygium patients were randomly selected to undergo surgical resection of pterygium lesion, and the normal margin of bulbar con-junctival tissues were collected as control. Gene expres-sion was detected quantitatively by the method of quantitative real-time PCR (QRT-PCR) analysis. RESULTS: The expression level of TGF-β1 and TGF-β2 was 4.26×10-7±1.45×10-7 and 1.08×10-10±0.68×10-10 in normal bulbar conjunctival tissues, while 10.67×10-7±7.47×10-7 and 8.23×10-11±6.63×10-11 in pterygium tissues. The expression level of TGF-βRⅠand TGF-βRⅡwas 0.003015±0.0036 and 5.33×10-5±5.05×10-5in normal bulbar conjunctival tissues, while 0.000379±0.000281 and 1.002×10-5±9.04×10-6 in pterygium tissues. The expression level of TGF-β1 and TGF-β2 in pterygium was elevated (P<0.01). TGF-β1 expression level in pterygium increase 2.9±2.8 times than in normal conjunctiva. TGF-β2 expression level in pterygium increase 7.5±1.4 times than in normal conjunctiva. The expression level of TGF-βRⅠin pterygium was significantly lower (P<0.05). The expression level of TGF-βRII in pterygium was significantly lower (P<0.01). CONCLUSION: QRT-PCR is an effective method to quantitatively detect gene expression in eye. The upregulation of TGF-β1 and TGF-β2 and downregulation of their receptors expression may play an important role in the pathogenesis of pterygium, which is noteworthy further investigation in diagnosis and treatment of pterygium.real-time PCR; gene expression
5.In vitro effects of acitretin on the apoptosis and expressions of insulin-like growth factor binding protein 7 and vascular endothelial growth factor in HaCaT cells
Fengmei XIANG ; Zhiping WEI ; Liansheng ZHONG ; Qing YANG ; Yanqun LIU
Chinese Journal of Dermatology 2014;47(7):494-498
Objective To investigate the in vitro effects of acitretin on the apoptosis and expressions of insulin-like growth factor binding protein 7 (IGFBP7) and vascular endothelial growth factor (VEGF) in HaCaT cells.Methods Cultured HaCaT cells were treated with various concentrations (10-5,10-64,10-7,10-8 mol/L) of acitretin for various durations,with those cultured in acitretin-free medium serving as the control group.Then,CCK-8 assay was performed to evaluate the proliferation of cells after 24-,48-and 72-hour treatment,flow cytometry to detect the apoptosis of HaCaT cells,and Western blot and reverse transcription-PCR to quantify the protein and mRNA expressions of IGFBP7 and VEGF in HaCaT cells,respectively,after 48-hour treatment.Statistical analysis was carried out by one-way analysis of variance and Pearson correlation analysis.Results The proliferation of HaCaT cells was inhibited by the treatment with acitretin,and the inhibitory effect increased with the elevation of concentration and prolongation of treatment duration of acitretin.A significant decrease was observed in the proliferative activity of HaCaT cells treated with acitretin of 10-8 mol/L for 48 hours,and when the concentration of acitretin was 10-5 mol/L,the proliferation of HaCaT cells was inhibited by 39.94% ± 2.27% and 49.77% ± 1.87% at 48 and 72 hours respectively,compared with the control cells.The HaCaT cells treated with acitretin of 10-5 mol/L for 48 hours showed a significant elevation in apoptosis rate (7.617% ± 0.767% vs.1.803% ± 0.313%,P < 0.05),IGFBP7 protein and mRNA expressions (0.939 ± 0.040 vs.0.436 ± 0.013,0.872 ± 0.079 vs.0.190 ± 0.056,both P < 0.05),but a significant reduction in VEGF protein and mRNA expressions (0.213 ± 0.032 vs.0.798 ± 0.036,0.274 ± 0.041 vs.0.933 ± 0.054,both P < 0.05) in comparison to the control cells.Conclusions Acitretin can induce the apoptosis of HaCaT cells,and up-regulate IGFBP7 but down-regulate VEGF expressions in HaCaT cells at protein and mRNA levels.
6.The clinic experience and literature review with 1 case of chronic tracheoesophageal fistula.
Qing CHENG ; Jingjing WEI ; Banghua LIU ; Yi ZHONG
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2014;28(23):1877-1879
OBJECTIVE:
To investigate the clinical features of chronic tracheoesophageal fistula (TEF), provided disease-related treatment experience and lessons for clinicians.
METHOD:
To successfully repair one case of chronic tracheoesophageal fistula with surgery, and to analyze the clinical treatment process, combined with relevant literature, the author reported the experiene of diagnosis and treatment in TEF.
RESULT:
After the gastrointestinal ostomy and Stent implantation, the fistula persisted, nine months later ,we took the surgery to repair the fistule, ten days postoperation, the fistule healed and esophageal iodine water examination didn't prompt obvious abnormalities, the patient was discharged without any postoperative complications 12 days postoperation.
CONCLUSION
If conservative treatment failed with TEF, the surgical repair should be carried timely, By double sutured with fistula in surgery, and reinforced with the approaching muscle tissue, It can achieve good results.
Humans
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Postoperative Complications
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Postoperative Period
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Tracheoesophageal Fistula
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pathology
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surgery
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Wound Healing
7.Effect of Guizhi Fuling capsule and combination of active ingredients on rats with uterine myoma.
Qing-qing HENG ; Liang CAO ; Na LI ; Gang DING ; Zhen-zhong WANG ; Wei XIAO
China Journal of Chinese Materia Medica 2015;40(11):2206-2209
It is to observe the therapeutic action of Guizhi Fuling capsule and the combination of active ingredients on model rats with uterine leiomyoma. The hysteromyoma rats models was established in rats by loading eatrogen, to observe the effect on pathological condition of uterus, uterus wet weight, the content of estradiol and progesterone. Guizhi Fuling capsule and the combination of active ingredients remarkably decreased uterus weight, restrained the excess proliferation of the smooth muscle of uterus, decreased the estraiol and progesterone in blood serum. Guizhi Fuling capsule and the combination of active ingredients can restrain the formation of hysteromyoma in a dose-dependent manner. Perhaps the combination of active ingredients is the material foundation of antihysteromyoma.
Animals
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Capsules
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Drugs, Chinese Herbal
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therapeutic use
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Estradiol
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blood
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Female
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Leiomyoma
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blood
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drug therapy
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pathology
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Progesterone
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blood
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Rats
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Rats, Wistar
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Uterine Neoplasms
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blood
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drug therapy
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pathology
8.Effects of HBV infection on hepatic fibrosis and level of Th1/Th2 cytokines in the patients with Schistosomiasis japonica
Zhong-Wei JIA ; De-Yong CHU ; Wei WANG ; Qing-Li LUO ; Ji-Long SHEN ;
Chinese Journal of Laboratory Medicine 2003;0(10):-
Objective The levels of Thl cytokines(IL-10 and IL-13)and Th2 cytokines(INF-? and TNF-?)were determined in the sera of patients with Schistosomiasis japonica in order to find the relationship between cytokines and severe hepatic fibrosis(HF)in schistosomiasis.Methods A total of 358 patients with advanced Schistosomiasis japonica were examined by ultrasound.68 HBsAg negative patients were chosen randomly as experimental control.Among them,39 patients were found to have mild HF and 29 were severe HF.The sera levels of Thl and Th2 cytokines were determined with ELISA.Results Among these 358 patients,83(23.2%)were HBsAg positive.Neither earlier nor severer hepatic fibrosis was noted in the patients who had been simultaneously infected with HBV than those only infected with schistosomiasis. There was a significant difference between mild[ 1.60(1.30-12.14)ng/L]and severe[ 4.20(1.43- 52.07)ng/L]HF patients in the level of IL-10(Z=-3.907,P0.05)was found in level of IFN-?,between severe[3.12(1.38-66.14)ng/L]and mild[5.87(1.33-216.33)ng/ L]HF subjects.Our observation did not reveal any obvious difference of TNF-? between severe[ 2.48(0.79 -19.86)ng/L]and mild[ 2.28(0.67-15.72)ng/L]HF groups.Conclusions Patients infected with advanced shistosomiasis may become more susceptible to HBV.The results of the present investigation showed that a high level production of IL-13 was associated with severe HF.
9.Effects of ligustrazine on blood rheology, aldose reductase and renal function in diabetic nephropathy rats
Juan ZHONG ; Wei QIN ; Yao QING ; rong Qing ZHONG ; yue Shu WU ; bo Lian WEI
Chinese Traditional Patent Medicine 2017;39(11):2231-2235
AIM To explore the effects of ligustrazine on blood rheology,aldose reductase (AR) and renal function in diabetic nephropathy (DN) rats.METHODS The DN rat model was established by intraperitoneal injection of streptozotoein (55 mg/kg),rats were randomly divided into five groups,model group,irbesartan [50 mg/(kg · d)] group,high-,middle-and low-dose of ligustrazine [200,100,50 mg/(kg · d)] groups,together with normal control group.All the rats received daily garage for eight successive weeks.At the end of experiment,blood rheology,blood glucose,aldose reductase in erythrocyte and kidney tissue,24 h urinary protein,blood urea nitrogen,creatinine,creatinine clearance and renal function were observed.RESULTS Compared with the model group,blood rheology,blood glucose and renal function in various treatment groups were effectively improved,and aldose reductase activity was significantly decreased (P < 0.05).HE staining and PAS staining showed that the pathological changes in kidney were significantly alleviated.CONCLUSION Ligustrazine can protect kidney of DN rats by ameliorating blood rheology,decreasing blood glucose and inhibiting aldose reductase activity.