ive] To explore a new method of evaluating the healing of fractures. [Methods] Self-con trolled trial was applied. Bone mineral density ( BMD) of fracture end (region 3, R 3 ) and its neighboring proximal and distal regions ( R 2 and R 1 respectively) in 126 cases of fracture of lower end of radius was deter mined by dual-energy X-ray absorptionmetry. [Results] BMD of R3 elevated as compared with that of R 2 and R1 (P

AIMTo investigate the role of mitochondrial permeability transition pore (MPTP) in the cardioprotection by remote preconditioning (RPC).

METHODSRemote Precondition (RPC) was induced in anesthetized male Sprague-Dawley rats by three cycles of 5 min of right femoral artery occlusion followed by 5 min of reperfusion. Myocardial ischemia/reperfusion (I/R) injury was achieved by ligation of the left anterior descending coronary artery for 30 min and then reperfusion for 120 min. Infarct size was determined by 2,3,5-triphenyltetrazolium chloride (TTC) staining method. The level of lactate dehydragenase (LDH) in plasma and the opening of the mitochondrial permeability transition pore (MPTP) were measured.

RESULTSRPC significantly decreased the infarct size and plasma lactate dehydrogenase level induced by I/R, and these effects were attenuated by atractyloside (Atr, 5 mg/kg), a MPTP activator. However, administration of cyclosporin A (CsA, 10 mg/kg), an inhibitor of MPTP, decreased the effect of I/R. In isolated ventricular myocytes loaded with calcein, RPC decreased the MPTP opening, and this effect was attenuated by Atr (20 micromol/L).

CONCLUSIONInhibition of MPTP opening is involved in the cardioprotection by RPC.

Animals ; Extremities ; blood supply ; Ischemic Preconditioning ; methods ; Male ; Mitochondrial Membrane Transport Proteins ; physiology ; Myocardial Reperfusion Injury ; physiopathology ; prevention & control ; Rats ; Rats, Sprague-Dawley

Objecfive To investigate antibiotic resistance,carbapenemase genotype and the molecular epidemiology of multidrug-resistant Acinetobacter baumannii (Aba) collected from 3 military hospitals in China.Methotis The minimum inhibitory concentrations (MIC) were examined by ager dilution method.Genotypes of carbapenemases were amplified by multiplex PCR and its products were sequenced.PCR was used to detect per gene,Homology of the resistant isolates was analyzed by pulse-field gel electrophoresis(PFGE).Results Among the 64 MDRA strains,78.1%(50)strains possessed blaOXA-23 gene,89.1%(57) carried Class 1 integrase gene,39.1% (25) with blaPER-1 gene,and 1 strain with blaOXA-58-like gene.PFGE showed that 13(A,B,C,D,E genotype) different clones were identified in these strains.A,B,and U clones were the predominant clones in three hospitals,respectitively.Conclusion OutbreaEs of muitidrug-rcsistant Aba occurred at 3 military hospitals with the most prevalent carbapenemase as OXA-23 enzyme.OXA-58 type of carbapenemase and per-1 in Aba were also isolated.

Aim The relationship between the therapeutic effect of TEA (total extract of astragalosides) on adjuvant arthritic (AA) rats and its antioxidative effects were studied . Methods The volume of non-injected hind paw of AA rats and malondialdehyde(MAD) content of arthritic synoviocytes from AA rats were measured and the proliferative responses of fibroblast, the level of superoxide anion() and the hydroxyl radical (?OH) generated in vitro were detected . Results The anti-inflammatory effect of TEA might be related to its antioxidative activity.In vitro low-level oxidative stress promoted the proliferative responses of fibroblast in rats synovium, which was marked by inhibited by TEA in a concentration-dependant manner. Further study showed that TEA could inhibit NBT reduction induced by both xanthine-xanthine oxidase and non-enzyme generated , but the inhibitory effect of this compound on activity of xanthine oxidase was obtained only at high concentration (more than 80 ?g?ml-1). It was also found that TEA could dose-dependantly inhibit the hydroxylation of benzoic acid induced by Fenton reaction generated ?OH. Conclusion TEA has significant therapeutic effects on AA rats, which might be related to its antioxidative effects

Objective:To investigate the clinical application of placental growth factor (PLGF) combined with uterine artery pulsation index in predicting early-onset preeclampsia.Methods:From March 2018 to March 2019, 69 cases of early-onset preeclampsia in the Maternal and Child Health Hospital of Jiaxing were selected as observation group, including 37 cases in mild group and 32 cases in severe group.And 58 cases of normal pregnant women in our hospital from March 2018 to March 2019 were selected as control group.The changes of plasma PLGF and uterine artery index, the changes of plasma PLGF and uterine artery index in different degrees of illness, and the diagnostic sensitivity and specificity of PLGF combined with uterine artery index were compared between the two groups.Results:The level of plasma PLGF in the observation group was (1.29±0.25)μg/L, which was lower than that in the control group[(1.70±0.34)μg/L]( t=7.816, P<0.05). The PI (1.48±0.31), RI (0.83±0.12) and S/D(2.97±0.65) of the observation group were higher than those of the control group[(0.91±0.18), (0.58±0.09) and (1.71±0.53)]( t=12.357, 13.071, 11.823, all P<0.05). The level of PLGF in the severe group was (1.13±0.27)μg/L, which was lower than that in the mild group[(1.45±0.23)μg/L]( t=5.317, P<0.05). The PI(1.71±0.36), RI(0.97±0.14) and S/D(3.45±0.71) in the severe group were higher than those in the mild group[(1.16±0.24), (0.72±0.10) and (2.43±0.57)]( t=7.556, 8.618, 6.616, all P<0.05). The diagnostic sensitivity and specificity of PLGF combined with uterine pulsation index were higher than those of PLGF and uterine pulsation index alone. Conclusion:PLGF combined with uterine pulsation index can predict the condition of early-onset preeclampsia, and the combination can improve the sensitivity and specificity.

The present study was undertaken to investigate the linkage between angiotensin-(1-7) ［Ang-(1-7)］ and the release of amino acid neurotransmitters in the the rostral ventrolateral medulla (RVLM) by techniques of microinjection, microdialysis combined with high performance liquid chromatography (HPLC)-fluorescent detection. Unilateral microinjection of Ang-(1-7) into the RVLM of anesthetized rats produced an increase in mean arterial pressure (MAP) accompanied by an increased release of glutamate (Glu). In contrast, microinjection of Ang779, a selective antagonist of Ang-(1-7) receptor, caused a decrease in MAP with a decreased releaceof Glu and an increased release of glycine,taurine and r-aminobutyric acid.The pressor effect of Ang-(1-7)and the depressor effect of Ang779 were in part blocked by corresponding andtagonists of aminoacid receptors.These results suggest that the pressor effect of Ang-(1-7)in the RVLM may be partially due to an increased release of Glu,whereas the depressor effect of Ang779 may be partially attributed to a decreased release of Glu and an increased release of inhibitory amino acid neurotransmitters

OBJECTIVETo study the mode of transmission and molecular characteristics on carbapenem-resistant Acinetobacter baumannii strain. Strains were isolated from different parts of samples in various patients.

METHODSClinical information of carbapenem-resistant Acinetobacter baumannii isolates were stored and analyzed by WHONET 5.4 software. The transmission and pathopoiesis of the strains were learned through case file review. Genotypes of isolates were identified by pulse-field gel electrophoresis (PFGE) and genes of carbapenemase were detected by multiple PCR, in order to find molecular characteristics and relatedness between strains.

RESULTS29 stains of Acinetobacter baumannii resistant to carbapenem were isolated from 2 or more kinds of samples among 13 patients'. Two genotypes were identified by PFGE: genotype A was obtained from 22 isolates in 11 patients and genotype B was obtained from 7 isolates in 4 patients. PCR amplification showed that all strains possessed OXA-23 gene except 1, and all strains possessed Integrase gene I except 3.

CONCLUSIONThere were 2 different genotypes from 29 strains of carbapenem-resistant Acinetobacter baumannii with Genotype A as the main type. OXA-23 carbapenemase gene and integrase gene I were detected from most of the isolates. All the strains could be easily transmitted in the body of the patients and among patients, hence becoming the epidemic pathogen of iatrogenic infection.

Acinetobacter Infections ; microbiology ; transmission ; Acinetobacter baumannii ; classification ; drug effects ; genetics ; Carbapenems ; pharmacology ; Cross Infection ; microbiology ; transmission ; Drug Resistance, Bacterial ; genetics ; Genotype ; Humans

To investigate the urination-reducing effect and mechanism of Zhuangyao Jianshen Wan (ZYJCW). In this study, SI rats were subcutaneously injected with 150 mg · kg(-1) dose of D-galactose to prepare the sub-acute aging model and randomly divided into the model group, the Suoquan Wan group (1.17 g · kg(-1) · d(-1)), and ZYJCW high, medium and low dose groups (2.39, 1.20, 0.60 g · kg(-1) · d(-1)) , with normal rats in the blank group. They were continuously administered with drugs for eight weeks. The metabolic cage method was adopted to measure the 24 h urine volume and 5 h water load urine volume in rats. The automatic biochemistry analyzer was adopted to detect urine concentrations of Na+, Cl-, K+. The ELISA method was used to determine serum aldosterone (ALD) and antidiuretic hormone (ADH). The changes in P2X1 and P2X3 mRNA expressions in bladder tissues of rats were detected by RT-PCR. According to the results, both ZYJCW high and medium dose groups showed significant down-regulations in 24 h urine volume and 5 h water load urine volume in (P <0.05, P <0.01), declines in Na+ and Cl- concentrations in urine (P <0.01), notable rises in plasma ALD and ADH contents (P <0.05, P <0.01) and remarkable down-regulations in the P2X1 and P2X3 mRNA expressions in bladder tissues (P <0.01). The ZYJCW low dose group revealed obvious reductions in Na+ and Cl- concentrations in urine (P <0.01). The results indicated that ZYJCW may show the urination-reducing effect by down-regulating the P2X1 and P2X3 mRNA expressions in bladder tissues of rats with diuresis caused by kidney deficiency.
Aging ; physiology ; Animals ; Diuresis ; drug effects ; Drugs, Chinese Herbal ; pharmacology ; Female ; Gene Expression Regulation ; Kidney Diseases ; drug therapy ; metabolism ; RNA, Messenger ; analysis ; Rats ; Rats, Sprague-Dawley ; Receptors, Purinergic P2X1 ; genetics ; Receptors, Purinergic P2X3 ; genetics ; Urinary Bladder ; metabolism

OBJECTIVETo study the effect of Trichinella spiralis (T.spiralis) infection on the expression and distribution of colonic epithelial E-cadherin in mice and its mechanism.

METHODSBALB/c mice and STAT6-/- mice were infected with T.spiralis, and mice without infection were used as control. Seven days later, the horseradish peroxidase (HRP) was infused by rectal enema. Serum HRP was detected in the subsequent 0, 60 and 120 minutes. Then the mice were sacrificed and colon was taken out. The distribution of E-cadherin in colon was detected by immunofluorescence staining, and the expression of E-cadherin was detected by Western blot. The expression of interleukin-4 (IL-4) in mesenteric lymph nodes was detected by ELISA.

RESULTSSerum HRP level in infected BALB/c mice was significantly higher than that in control mice (P<0.05), while it was not significantly different between infected STAT6-/- mice and controls (P>0.05). In infected BALB/c mice, E-cadherin located in cytoplasm of colonic epithelial cells, while in controls, it located in cellular membrane. E-cadherin expression down-regulated significantly in infected BALB/c mice as compared to controls. E-cadherin expression and distribution did not change obviously in infected STAT6-/- and control mice. IL-4 level in mesenteric lymph nodes of infected BALB/c mice [(193.0±12.5) μg/L] was significantly higher as compared to control BALB/c and infected STAT6-/- mice [(21.0±2.3) μg/L and (15.0±3.1) μg/L, all P<0.05].

CONCLUSIONT.spiralis infection can increase colonic epithelial permeability of mice, which may be associated with induction of Th2 cytokine secretion.

Animals ; Cadherins ; metabolism ; Colon ; metabolism ; Disease Models, Animal ; Female ; Interleukin-4 ; metabolism ; Intestinal Diseases, Parasitic ; metabolism ; Intestinal Mucosa ; metabolism ; Lymph Nodes ; metabolism ; Mice ; Mice, Inbred BALB C ; Mice, Knockout ; Trichinella spiralis ; Trichinellosis ; metabolism

Objective This study was to estimate the prevalence of elderly mistreatment (EM) in rural community and to examine the association between social support and the risk of the EM.Methods A cross-sectional descriptive study was performed in three rural communities (17 villages) in Macheng city of Hubei province.2000 subjects aged 60 years or older were selected using cluster sampling.Questionnaire being developed would include general information,a scale to measure social support,and a modified vulnerability on abuse screening scale (VASS) to measure the EM.Results The prevalence rates of EM,physical abuse,emotional abuse,neglect and financial exploitation for rural elderly people in Macheng city were 36.2％,4.9％,27.3％,15.8％,and 2.0％respectively.After adjusting for potential confounding factors,necessary practical support from family (OR=1.28,95％CI:1.01-1.63) was the risk factor causing EM while having got practical support (OR=0.76,95％CI:0.58-0.98) or moral support (OR=0.63,95％CI:0.49-0.82) from family and moral support from friends (OR=0.73,95％CI:0.59-0.90) when in need were the protective factors.The protective factors on physical abuse,emotional abuse,neglect and financial exploitation would include:getting practical support from family when in need (OR=0.59,95％CI:0.35-0.99),getting moral support from family (OR=0.67,95％ CI:0.51-0.89) and friends (OR=0.67,95％ CI:0.54-0.84) and getting practical support from family when in need (OR=0.63,95％CI:0.45-0.88),getting practical support from family ( OR =0.38,95％ CI:0.14-0.98 ) and getting moral support from friends when in need (OR=0.42,95％CI:0.20-0.87),respectively.Conclusion High prevalence of EM was seen in the rural areas of Macheng city.Social support was an important protective factor for EM in this population.