3.Analysis of the results detected by ultrasound biomicroscopy before patients with rhegmatogenous retinal detachment perform pars plana vitrectomy
Juan, CHEN ; Zhong-Ping, CHEN ; Qi-Chang, WANG ; Xin, HE
International Eye Science 2015;(5):900-903
?AlM: To analyze the result of ultrasound biomicroscopy ( UBM ) of patients with rhegmatogenous retinal detachment ( RRD ) who need to perform pars plana vitrectomy ( PPV ) to explore the value of clinical application of UBM at preoperation.
?METHODS:Patients who underwent PPV for RRD in our hospital from January to December 2014 were selected. The preoperative results of UBM were recorded and the positive findings of UBM were analyzed, the value of preoperative application of UBM was investigated.
?RESULTS:Totally 356 cases (357 eyes) of patients with RRD were received PPV. All patients were examined by UBM, 122 eyes ( 34. 17%) were positive, 56 eyes were ciliochoroidal detachment, 28 eyes were narrow anterior chamber angle, 6 eyes were chamber angle-closure, 28 eyes were ciliary body cyst, 4 eyes were iris cyst, 11 eyes were anterior proliferative vitreoretinopathy, 1 eye was retinal cyst. There were 12 eyes existing two lesions at the same times. ln this study, patients with choroidal detachment was detected by UBM in 56 eyes, the positive rate was 15. 7%, while 25 eyes was detected by B -ultrasonography, the positive was 7. 0%. The difference was statistical significant(χ2=13. 382, P<0. 05).
?CONCLUSlON: The patients with RRD have underwent PPV for the condition of illness, preoperative UBM can be examined to detect the condition of anterior chamber angle, iris, ciliary and anterior choroid, which is significant for comprehensively understanding the preoperative condition of patients, estimating the difficulty of the operation, and guiding operation and the postoperative follow-up.
4.Study of regulation of miR-21 on ADAMTS-1 expression
Hong ZHAO ; Pingbo YAO ; Li DAI ; Ping ZHONG ; Jun HE
The Journal of Practical Medicine 2015;31(15):2426-2429
Objective To investigate the regulatory effect of miR-21 on ADAMTS-1 expression in pulmonary fibrosis model. Methods A mouse model of pulmonary fibrosis was established using bleomycin (BLM) anda model of pulmonary fibrosis was constructed in vitro , the expression level of miR-21 was measured by quantitative real-time polymerase chain reaction (qRT-PCR), while the protein expression of ADAMTS-1 was measured by Western blot. NIH3T3 were transfected with miR-21 mimics and inhibitor in vitro and the cellular expression of ADAMTS-1 was measured by Western blot. Results Compared with the blank group , in mouse models of pulmonary fibrosis , the miR-21 expressions in lung tissues at three time points after BLM-treatment were significantly up-regulated while an evident decrease in ADAMTS-1 expressions were observed (P < 0.01). In vitro pulmonary fibrosis model , NIH3T3 cells after TGF-β1 in concentration 5 μg/L stimulation down-regulated ADAMTS-1 expression and up-regulated miR-21 expression (P < 0.01). NIH3T3 transfected with miR-21 mimics and inhibitor, up-regulated miR-21 expression, while down-regulated ADAMTS-1 protein expression. Conclusions Up-regulation of miR-21 and Down-regulation of ADAMTS-1 might be involved in the progression of pulmonary fibrosis model; miR-21 could negatively regulate ADAMTS-1 expression.
5.Coal worker's pneumoconiosis complicated with lung cancer patients with respiratory tract of drug resistance of Pseudomonas aeruginosa.
Ji-Zhong CHEN ; Fu-Ping JIN ; He-Hong FAN
Chinese Journal of Industrial Hygiene and Occupational Diseases 2011;29(7):548-550
Adult
;
Aged
;
Aged, 80 and over
;
Anthracosis
;
complications
;
microbiology
;
Anti-Bacterial Agents
;
pharmacology
;
Drug Resistance, Bacterial
;
Humans
;
Lung Neoplasms
;
complications
;
microbiology
;
Male
;
Middle Aged
;
Pseudomonas Infections
;
microbiology
;
Pseudomonas aeruginosa
;
drug effects
6.Acute myocarditis misdiagnosed as "acute cholecystitis".
Ying CHEN ; Wei-qiang ZHENG ; Jian-ping ZHONG ; Jian-jun WANG ; Miao-xia HE
Chinese Journal of Pathology 2010;39(2):114-116
Acute Disease
;
Adult
;
Cholecystitis, Acute
;
diagnosis
;
pathology
;
Diagnostic Errors
;
Female
;
Humans
;
Myocarditis
;
diagnosis
;
pathology
7.Identification of liposoluble constituents in Yushu tablets by UPLC-ESI-IT-TOF/MS.
Zhong-ting XIA ; Li-ying BAO ; Yi HE ; Yan-ru DENG ; Shui-ping ZHOU
Acta Pharmaceutica Sinica 2015;50(5):587-593
In order to identify the chemical constituents of Yushu tablets comprehensively, we studied the chemical constituents of CHCl3 extract from Yushu tablets by the ultra performance liquid chromatography-electrospray ionization-ion trap-time of flight mass spectrometry (UPLC-ESI-IT-TOF/MS). It showed that there were more than 100 compounds separated, and forty-nine peaks among these were identified on the basis of high resolution mass spectrometry data and literature data reported. Determination of twelve peaks was further confirmed by standard substances. These components assigned to the different plant sources mainly included phenylpropanoids, triterpenoids, quinones and m-trihydroxybenzene compounds. By analyzing the chemical components of CHCl3 extract from compound Chinese medicine Yushu tablets comprehensively, this study provided the foundation for studying chemical components, pharmacodynamic substance and quality control of Yushu tablets.
Chromatography, High Pressure Liquid
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Chromatography, Liquid
;
Drugs, Chinese Herbal
;
analysis
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Spectrometry, Mass, Electrospray Ionization
;
Tablets
;
Tandem Mass Spectrometry
8.A novel pore-forming hemolysin protein in Leptospira species
Yang YANG ; Jinhong QIN ; Yi ZHONG ; Ping HE ; Baoyu HU ; Xiaokui GUO
Chinese Journal of Zoonoses 2009;(9):831-836
Hemolysins of Leptospira interragans have been shown to be the virulence factor in the pathogenesis of leptospirosis and 10 potential hemolysin genes were charecterized by genomic annotation of L.interrogans serovar.Lai strain 56601. In the present study, the LA0202 gene supposed to encode one of the new potential hemolysin was cloned and the protein encoded was purified. The purified protein was shown to have highly hemolytic activity as demonstrated on the sheep blood agar plate. It was also confirmed that the LA0202 protein-mediated hemolysis on sheep erythrocytes was osmotically protected by PEG6000. Meanwhile, this protein could induce pore formation on sheep erythrocytes and cause damages on the membrane of human L-02 liver cells. In addition, it could induce apoptosis of human L-02 liver cells after treatment of cells with this protein for 24 hours. It is evident that LA0202 protein acting as a pore-formong hemolysin can induce cytotoxic damage on mammalian cells.
10.A Clinical Study of Faropenem on Treatment of Acute Bacterial Infections
Ping HE ; Demeng LIU ; Shiduo SONG ; Liying CUI ; Yaping MENG ; Tianduo CHEN ; Ganping ZHONG ; Shuwen YANG
Tianjin Medical Journal 2009;37(10):849-852
Objective: To evaluate the clinical efficacy and safety of domestic faropenem in the treatment of acute bacterial infections. Methods: A multicenter, randomized, double blind and double simulation clinical study was conducted to compare the efficacy and safety of faropenem and cefaclor in the treatment of acute bacterial infection. Patients in trial group(n = 122) were given faropenem 250 mg,and in control group (n = 118) were given cefaclor 200 mg,3 times daily for 7 to 10 days.Results: The clinical cure rates were 33.61% and 27.12% in trail and control groups respectively and the clinical effective rates were 87.70% and 83.05% respectively. There was no significant difference in terms of clinical effectiveness between the two groups(P > 0.05). The adverse reaction rates were 7.32% in trial group and 3.36% in control group(P > 0.05). The adverse reaction of the trial group was mainly exaltation of aminotransferase, which did not affect the therapy. No severe adverse reaction was found.Conclusion: Domestic faropenem is effective and safe for the treatment of bacterial respiratory tract and urinary tract infections.