Objective:To construct and evaluate a Salmeterol lungs-specific liposomal delivery system.Methods:①Liposome were prepared by the reverse-phase evaporation method and instilled into the tracheas of SD strain rats.The influence of Salmeterol on the uptakes of liposome was evaluated.②pEGFPC1(25 ?g/rodent) encapsulated in lungs-specific liposome were administered intravenously into Guinea pigs,GFP expression were observed by means of fluorescent microscopy 24h and 48h after administration.Results:①2,4,8,12,16 hours after instillation,lungs-specific liposome uptake were significantly higher than nonspecific liposome uptake(P

Purpose:To establish a human large cell lung cancer multi-drug resistance cell line H460/cDDP and explore its biological characteristics. Methods:A resistant human larget cell lung cancer cell line (H460/cDDP) was established by intermittent high dose cisplatin selection from the parental cell line H460. Drug sensitivity was detected by MTT assay. The changes of its biological characteristics were determined using light microscopy, Trypan Blue staining rejection, cell counting, chromatosome analysis; Neoplasia formation test in nude mouse was performed to investigate its in vivo characteristic. Results:H460/cDDP cell line was developed after about 6 months and the resistance index to cisplatin was 10.21. H460/cDDP cells exhibited cross-resistance to 5-Fuorouracil, adriamycn, etoposide and methotrexate. Compared with the parent cells, the morphology wac changed; doubling time prolonged (from 20.78h to 36.46h), while the chromatosome number and caryotype were similar. After being frozen, deposited and resuscitated repeatedly, its biological characteristics remained stable. It had neoplasia formation ability in vivo, but the forming time was longer than its parental cell. Conclusions:The newly established multi-drug resistant large cell lung cancer cell line H460/cDDP cell line possessed the typical multi-drug resistant phenotype. It was stable and had neoplasia formation ability in vivo,suitable for research.

AIM: To investigate the level of ET-1 produced by cultured human bronchial epithelial cells (HBECs) under injury and the effects of injured HBECs on ET-1 production in sub-epithelial fibroblasts. The interaction between ET-1 and matrix metalloproteinase-9(MMP-9) was detected in HBECs under damage. The purpose of the study is to evaluate the effect of injured HBECs related to ET-1 release on airway remodeling in asthma. METHODS: ET-1 level was detected in supernatants from cultured HBECs 12 h after being treated with either mechanical scraping or LPS stimulation or mechanical scraping plus LPS, as well as from subepithelial fibroblasts cocultured with mechanical damaged HBECs. It was also measured in the supernatant from HBECs transfected with MMP-9 expression plasmid. MMP-9 activity was assessed in supernatants from HBECs stably transfected with pEGFPc1 -antisense-ET-1 converting enzyme(ECE) RNA. RESULTS: It was found that there was an increase in ET-1 level in supernatants from HBECs either treated with mechanical scraping plus LPS or transiently transfected with MMP-9 plasmid, as well as from sub-epithelial fibroblasts cocultured with mechanical scraping HBECs compared with that in controls. Gelatin zymography showed a obviously attenuated gelatinolytic activity of MMP-9 in conditioned media of HBECs expressing antisense ECE RNA after mechanical damage. CONCLUSIONS: Airway epithelial cells under injury are able to overproduce ET-1 as well as initiate ET-1 release from sub-epithelial fibroblasts, MMP-9 produced by injured bronchial epithelial cells may also increase ET-1 processing leading to ET-1 production further. The interaction between ET-1 and MMP-9, both of which enhanced in damaged HBECs, may play an important role in airway inflammation related to airway remodeling in asthma.

Objective To study of applied anatomy of the palmaris brevis musculocutaneous flap and to repair of pulp defect of thumb by free the palmaris brevis musculocutaneous flap. Methods The distributions of vascularis and nerves of the palmaris brevis musculocutaneous flap were observed on 30 upper limbs which were injected red latex. Nine cases who sufferd from pulp defects of thumb were treated by free the palmaris brevis musculocutaneous flap. Results There were four sources of blood supply to palmaris brevis musculocutaneous flap ;ulnar arterial trunk,musculocutaneous and cutaneous branches of superficial palmar branch,the musculocutaneous branches from pro fund branch of ulnar artery and the proper volar ulnar digital artery of little finger..The venous drainages of the flap were venae comitantes of arteries mentioned above, nine cases who suffered from pulp defects of thumb were treated by free the palmaris brevis musculocutaneous flap successfully. Conclusions The palmaris brevis musculocutaneous flap and pulp of thumb have similar characteristics. The blood supply to the palmaris brevis musculocutaneous flap was rich,it could be used as a sensory flap and was one of good donor regions for repairing large pulp defects of thumb.

Objective To clarify the relationship between the first metatarsal web space and associated vessels and its application in dissection of the toes for thumb and finger reconstruction. Methods The relationships of the first dorsal metatarsal artery to the first deep transverse metatarsal ligament and the extensor expansion were observed on 42 adult cadaver lower limbs. Clinically 36 cases of thumb defects were reconstructed using the method of tracing the first dorsal metatarsal artery around the space of extensor expansion to dissect toes. Results The distal segment of the first dorsal metatarsal artery of Gilbert type I and type Ⅱwas located superficially to the layer of the extensor expansion.The time of harvesting the toe was shortened from 90 minutes to 50 minutes with 100%survival of reconstruction. Conclusions The distal segment of the first dorsal meatarsal artery lies constantly to the superficial layer of the extensor expansion.Consequently the location of the first metatarsal artery of Gilbert type I and type Ⅱbecomes much easier, by adopting the method of combination of sequential dissection and reverse dissection around the space of the extensor expansion.

Objective To clone and analyze the cDNA of major allergen Der f 2 of Dermatophagoides farinae in south China. Methods cDNA of Der f 2 was cloned by RT-PCR, screened and their sequences were analyzed. Results cDNAof Der f 2 was cloned. The sequence of the cloned Der f 2 was different with that published (D10448) in GenBank, with 87 additional nucleotides inserted into the 62th nucleotide of the original one. According to the original ORF, the deduced amino acids that were located prior or after the inserted 29 amino acid sequence showed no changes. Conclusion The cDNA of Der f 2 was cloned from Dermatophagoides farinae and its sequence showed significant difference with that reported in the GenBank.

Objective To propose the concept of “Diffusion Index”to replace Oxygenation Index as independent indicators to evaluate prognosis on ARDS patients under mechanical ventilation treatments,and comparison carried out between them preliminarily.Methods Calculation of “1 000 × (PaO2 /FiO2 /PEEP)”was taken as “Diffusion Index”.A total of 130 ARDS patients under mechanical ventilation support (MVS)were admitted to Peking Union Medical College Hospital ICU from July 2013 to July 2014.The data of these patients were retrospectively analyzed.Of them,15 patients were excluded because these patients refused invasive ventilation support.Respirator parameter setting and haemogas figures were recorded accordingly. Both Diffusion Index and Oxygenation Index were used separately to predict detachment of MVS from patients in 28 days,and the correlation between these two indexes and ARDS prognosis were determined.Results According to the outcomes of patients in 28 days,patients were divided into 3 groups:detached group (n =44),failed to detach group (n =14)and death group (n =57).There was obvious difference in trend diagrams observed among three groups between diffusion index and oxygenation index.COX regression analysis of survival curve demonstrated that if Diffusion Index kept greater than 405.8,probability of detachment of MVS from patients was higher and the correlation was significant (P =0.009 ).Conclusions Compared with Oxygenation Index,“Diffusion Index” is a comprehensive indicator for ARDS prognosis with better reliability and validity.

Objective To study the risk factors associated with death of patients suffered from acute exacerbation of interstitial pneumonia (AE-IP) with very high mortality and very difficult to handle so as to find the most suitable treatment strategy for these patients.Methods The data of 26 patients with AE-IP admitted to Emergency Intensive Care Unit in Peking Union Medical College Hospital from September 2010 to September 2013 were restrospectively analyzed.Comparison of general condition of patients,treatment strategy and response to non-invasive ventilation of patients was made between survival group and death group.Results There was no significant difference in general condition of patients between death group and survival group.But compared with idiopathic pulmonary fibrosis (IPF),connective tissue disease-related interstitial pneumonia had a tendency to accounting for higher proportion in the survival group.The rats of endotracheal intubation and invasive ventilation were significantly increased in death group (14％ vs.84％,P ＜ 0.05).There was significant improvement in PaO2/FiO2 after non-invasive ventilation for 2 hours in the survival group (176 ± i10 vs.218 ±64,P ＜0.05) while the death group had no significant improvement.Conclusions In patients with AE-IP,connective tissue disease associated interstitial pneumonia might have better outcome than IPF.AE-IP patients have a very high mortality rate once patients intubated,thus the decision of intubation in such patients needs to be very cautious.The patients with negligible improvement in PaO2/FiO2 after non-invasive ventilation for 2 hours may have a poor prognosis.

BACKGROUND:Methods after mechanical stimulation can be combined with various kinds of cells to produce many conversion types so as to start the various physiological functions of the body. OBJECTIVE:To analysis the reason why rol ing massage can improve the intracellular concentration of Ca2+METHODS:A biodynamic experimental device was set up, composed of pressure generating device, pressure forming device, pressure display device and related data recording device. We detected the effects of rol ing massage on Ca . 2+concentration in normal skeletal muscle cells and damaged cells through the different functions of the experimental device and system software. These functions consisted of pressure display, data transmission and recording, signal acquisition and processing, pressure signal acquisition, control and processing of signal acquisition, wireless signal transmission and receiving system, display system, storage system, system implementation function. RESULTS AND CONCLUSION:The experiment results showed that rol ing massage could reduce the Ca 2+concentration in the normal skeletal muscle cells and damaged cells. These findings suggest that rol ing massage can improve the overload of intracellular Ca2+in damaged cells, and it is superior to the sinusoidal mechanical stimulus.

Objective To investigate the clinical manifestations and MR imaging features of olivopontocerebellar atrophy(OPCA).Methods The clinical data and MR findings of 8 patients with OPCA (OPCA group) were retrospectively analyzed,and 75 cases of healthy physical examination people were chosen as control group (they were divided into 21-30 years group,31-40 years group,41-50 years group,51-60 years group,61-70 years group,and 15 cases in each group),all subjects under went pons measurement.Results All 8 patients with OPCA were sporadic cases.The cerebellar symptom,autonomic symptoms and extrapyramidal symptoms were common manifestations.The head MR imaging of OPCA presented with cerebellum and brain stem atrophy,cross sign was the characteristic appearance.Vertical diameter and anteroposterior diameter of the pons in OPCA group patients was (20.63 ± 2.16) and (16.74 ±4.37) mm,compared with control group [(27.14 ±1.73) and (22.28 ± 1.49) mm],there was significant difference (P ＜ 0.01).In control group,vertical diameter and anteroposterior diameter of the pons maximum in 21-30 years group and 31-40 years group,increased with age,the value had been reduced,but there was no significant difference (P ＞ 0.05).Conclusion MR is the first choice for the diagnosis of OPCA,combine with clinical manifestations may improve the diagnostic accuracy.