Objective To observe the therapeutic effect of thalidomide(Thd)on rheumatoid arthritis and its relation with the expression of hypoxia-inducible factor-1?(HIF-1?).Methods Female SD rats were randomly divided into two groups:a normal group and a model group.Each group was further divided into three subgroups:the control group,the vehicle group and the Thd group.The model group was subjected to collagen-induced arthritis.The Thd group and the vehicle group were given Thd(600 mg/kg)once daily and its vehicle DMSO respectively.The profile of arthritis was assessed by observing the clinical symptoms and histopathology and a qualitative clinical score of arthritis.The expression of HIF-1?in synovial tissuses was further detected by Western blot and immunohistochemistry technics.Results Compared to the control group with arthritis,clinical signs and pathological changes of arthritis were improved one and two weeks after ad- ministration of Thd respectively,and the qualitative clinical score of arthritis was evidently lowered.The ex- pression of HIF-1?in the inflammed synovial tissuses was found to be enhanced(P

The painless needle insertion technique, summarized by Professor WU Lian-zhong during his decades of acupuncture clinical practice is introduced in this article, which is characterized as soft, flexible, fast, plucking and activating antipathogenic qi. The Sancai (three layers) lifting and thrusting manipulation technique is adopted by Professor WU for getting the qi sensation. And features of 10 kinds of needling sensation such as soreness, numbness, heaviness, distension, pain, cold, hot, radiation, jumping and contracture are summarized. Finger force, amplitude, speed and time length are also taken as the basis of reinforcing and reducing manipulations. Moreover, examples are also given to explain the needling technique on some specific points which further embodies Professor WU's unique experiences and understandings on acupuncture.
Acupuncture Therapy ; history ; methods ; China ; History, 20th Century ; History, 21st Century ; Humans ; Needles

Objective To observe the short term therapeutic effects of continuous subcutaneous insulin infusion (CSII) in treatment of type 1 diabetic and type 2 diabetic patients,and compared with intensive therapy of insulin multiple subcutaneous injection by conventional scheme.Methods 48 diabetic patients including 14 cases of type 1 and 34 cases of type 2,were randomly divided into CSII group and control group. In CSII group,the short acting Novolin was subcutaneously infused using insulin pump continuously. In control group,the short acting Novolin was subcutaneously injected before three meals a day and medium acting Novolin was subcutaneously injected before sleep at night.Results Hyperglycemia of the CSII group was depressed,and decreasing degree was similar to that of the control group.However,therapeutic time,insulin dosage and insulin induced hypoglycemia reaction incidence in the CSII group were obviously lower than thoses in control group(P

OBJECTIVE:To establish the quality standard for Cangbai qutong capsule. METHODS:TLC was conducted to iden-tify the Rhizoma atractylodis,Asarum sieboldii,Paeoniae Radix Rubra and Cortex phellodendri;RP-HPLC was conducted to deter-mine the content of berberine hydrochloride. The column was Waters Symmetry-C18 with mobile phase of acetonitrile-0.1% phos-phoric acid (gradient elution) at flow rate of 1.0 ml/min,the detection wavelength was 265 nm,column temperature was 30 ℃, and the injection volume was 10 μl. RESULTS:TLC showed R. atractylodis,A. sieboldii,Paeoniae Radix Rubra and C. phelloden-dri had clear spots and good separation. The linear range of berberine hydrochloride was 21.12-84.48 μg/ml(r=0.999 6);RSDs of pre-cision,stability and reproducibility tests were lower than 3%;recovery was 96.439%-100.950%(RSD=1.34%,n=9). CONCLU-SIONS:The method is simple and reproducible,and can be used for the quality standards of Cangbai qutong capsule.

Adult ; China ; epidemiology ; Dust ; Humans ; Iron ; Male ; Metallurgy ; Occupational Exposure ; Pneumoconiosis ; diagnostic imaging ; epidemiology ; prevention & control ; Radiography ; Risk Factors ; Steel ; Welding

Aged ; Animals ; Diabetes Complications ; Diagnosis, Differential ; Female ; Humans ; Ophthalmoplegia ; diagnosis ; etiology ; Tolosa-Hunt Syndrome ; diagnosis

Objective:To explore the effect of fortified human milk feeding on growth and complica-tions of infants with extremely and very low birth weight (ELBW/VLBW)during hospital stay by a pro-spective,random and controlled study.Methods:In the study,1 22 ELBW/VLBW infants were enrolled and divided into two groups.The infants fed with human breast milk,combined with human milk fortifi-cation (HMF)during hospital stay were named HMF group (n=62),and those fed exclusively with pre-mature formula were named premature formula feeding group (PF group,n=60 ).The data of the in-fants’growth (the velocity of increase on the weight,length,head circumference and upper arm circum-ference),the time of rebounding to birth weight,the time of needing intravenous nutrition,the time of hospitalizing,the proportion of extrauterine growth retardation (EUGR)during hospital stay,the level of hemoglobin,bone metabolism and incidence of complications were compared between the two groups. Results:Among the 1 22 infants included,(1 )the length increment in HMF group was higher than PF group [(0.89 ±0.23)cm/week vs.(0.79 ±0.34)cm/week,P=0.04];there were no significant differences in the weight gain,head circumference increment and upper arm circumference increment (P>0.05);(2)the age of rebounding to birth weight [(1 0.1 3 ±4.03)d vs.(8.03 ±3.28)d,P=0.002]and the duration of intravenous nutrition [(1 6.77 ±6.63)d vs.(1 4.23 ±4.1 5)d,P=0.01 ] in HMF group were longer than that in PF group,there were no significant differences between the two groups in the hospital stay and age achieved feeding;(3 )there were no significant differences between the two groups in the incidence rate of EUGR during hospital stay (P>0.05);(4)the level of calcium at birth in HMF group was lower than that in PF group [(2.1 9 ±0.22)mmol/L vs.(2.32 ±0.27) mmol/L,P=0.005 ],and the level of alkaline phosphatase (AKP)in HMF group at discharge was higher than in PF group [(363.98 ±1 22.49)mmol/L vs.(299.73 ±1 1 7.39)mmol/L,P=0.004];(5)the incidence of the feeding intolerance (6.5% vs.1 8.3%,P=0.04)and sepsis (4.8% vs. 1 6.7%,P=0.03)in HMF group were less than in PF group,there were no significant differences be-tween the two groups on the morbidity of necrotizing enterocditis,retinopathy of prematurity (ROP),and bronchopulmonary dysplasia (BPD)(P>0.05).Conclusion:HMF for premature infants may ensure the same growth pattern as those fed by premature formula,promote the calcium absorption,decrease the inci-dence of sepsis and feeding intolerance,and does not increase the incidence of necrotizing enterocolitis.

Objective To establish the extraction process of Zhechong Chuangyu Capsule. Methods The difference of analgesic effect of water extraction and alcohol extraction in mice was observed by body-torsion test to determine the extract solvent. With the rate of aqueous extraction, n-butyl alcohol extraction and asperosaponin Ⅵ as evaluating indicator, the influencing factors including solvent volume, time and times of extraction were investegated to evaluate extracting procedure by orthogonal experiment. Results There was no obvious difference in analgesic effect between water extraction and alcohol extraction. Given the requirement of produce, aqueous extraction was a better choice. The optimum extracting condition was extracted 3 times with 20 folds volume of solvent, and extraction time was 150 minutes. Conclusion The extraction process is feasible to be applied into production.