OBJECTIVE:To study the preparation of fluconazole eyedrops as well as its quality control.METHODS:The content of fluconazole in eyedrops was determined with HPLC.The quality control of eyedrops has done in accordance with ChP.RESULTS:The average recovery was100.4%,RSD=0.54%(n=5).CONCLUSION:The formula of this preparation is reasonable with simple and convenient technology.Moreover,its quality control is easy to handle.

OBJECTIVE To study anatomical features of the cavernous segment of the internal carotid artery(ICA)via the extended transsphenoid approach under endoscope.METHODS Extended transsphenoid approach was performed on 11 adult cadaver heads.The appearance features of the cavernous segment of the ICA and its vessel branches were observed under endoscope.RESULTS The cavernous segment of the ICA can be divided into 5 segments and has 3 vessel branches.The optico-carotid recess is the landmark to define the ICA and optic nerve during operation.The optico-carotid recess is located between internal carotid and optic prominences,all of them are located on the lateral wall of the sphenoid sinus.CONCLUSION The cavernous segment of the ICA is the most important anatomic structure via the extended transsphenoid approach.The cavernous segment of the ICA,branch vessels,and important nerve tissue structures can be clearly visualized under endoscope via the extended transsphenoid approach.An extended transsphenoid approach with the help of an endoscope is an optimal surgical approach for the lesions that invade the internal wall of cavernous sinus.

Sulfur fumigation was a traditional maintenance method for traditional Chinese medicines (TCMs). However , as people have paid more and more attentions on the sulfur dioxide residue in the sulfur fumigated TCMs , China has gradually decreased and banned the sulfur fumigation for TCMs . This study adopted organic elemental analysis for the determination of sulfur contents in multiple TCMs . Elemental analysis can give accu-rate results with little sample amount in a short time . Data analysis indicated that the sulfur content of 0.5% can be set as a criterion for the identification of sulfur fumigated TCMs. Sulfur content of ten unknown TCMs were determined by elemental analysis and identified whether the TCMs have been fumigated by sulfur. The devel-oped elemental analysis method can be used as a screening method for rapid identification of TCMs' quality.

Objective To evaluate the MRI features of osteosarcoma.Methods 38 cases of osteosarcoma proved pathologically were retrospectively analyzed.All of them were examined by plain MRI scan,25 cases were examined by enhanced scan.Results The diagnostic accuracy of osteosarcoma of MRI was 93.9%.There were 30 cases of the extension into the medullary cavity,18 cases of soft tissue mass,10 cases of the destruction in epiphysis and 4 cases of the invasion in articular cartilage,especially T2 weighted STIR image and enhanced MRI scans were advantageous in showing the images mentioned above and in judging of the change after treated.Conclusion MRI is very important in diagnosis and clinical therapy of osteosarcoma.

Purpose:To investigate the relationship between HELICOBACTER PYLORI (Hp) and gastric carcinoma, as well as the possible carcinogenic mechanism by Hp. Methods: DNEL technique and immunohistochemical staining were used in the research to study the state of apoptosis, proliferation and p53 gene expression. Total 100 gastric mucosal biopsy specimens, including normal mucosa, Hp negative and Hp positive gastric precancerous lesions as well as gastric carcinomas were studied. Results:There were several apoptotic cells in the superficial epithelium and a few proliferative cells within neck of gastric glands, and no p53 protein expression in normal mucosa. In gastric carcinoma, apoptotic cells accounted for 1.62%, while proliferative cells 4.99%, and P53 protein significantly increased. in metaplasia, the apoptotic index (AI), proliferative index (PI) and positivity of p53 expression in Hp positive group were higher than those in normal mucosa ( P

Objective To evaluate food intolerance sensitivity among 2962 health check-up receivers from the First Hospital of China Medical University.Methods Serum samples of 2962 adults were collected from January 2008 to June 2010.Serum allergen-specific IgG antibodies of 14 kinds of food were detected by ELISA.The data were analyzed by using One-way analysis of variance.Results In this study,the rate of food intolerance was 0.4%(mushrooms) to 38.0%(eggs).Higher rates of food intolerance were found in egg,crab,shrimp,milk,and cod fish(Eggs 32.5%,crabs 26.0%,shrimps 19.8%,milk 13.8%,and cod fish 12.0% respectively).However,wheat,pork,and mushroom showed relatively lower rates of food intolerance(Wheat 0.8%,pork 0.4%,and mushrooms1.4% respectively).Positive reaction to food intolerance was even stronger in women(9.4% versus 8.2%).Conclusion It is discovered by study that in general population,the diversity of intolerance among different food is associated with 4 factors: gender; age; feature of food; individual diversity.It is recommended that the status of intolerance to food in the general population be investigated by health management.

AIM: To establish a method for determing camphor and eucalyptol in Shidi Water(Camphor,Rhizoma Zingiberis,Fructus Foeniculi,Cortex). METHODS: The chromatographic condition,DB-WAX capillary column(30 m?0.53 mm,1 ?m).The column temperature programming was from 65 ?C to 155 ?C(at rate of 6 ?C/min).FID Detection.The temperatrue was at 230 ?C. RESULTS: The linear range of camphor was within 0.18 mg/mL-5.78 mg/mL(r=1.000 0,n=6).The average recovery was 98.05%,RSD=0.94%.The linear range of eucalyptol was within 0.06 mg/mL-1.92 mg/ml(r=(0.999 7),n=6).The average recovery was 98.99%,RSD=1.31%. CONCLUSION: This method can be used for quality control of Shidi Water

Objective To investigate the effects of tropicamide and esculin-digitalisglycosides combination eye drops on low myopia of children (age ranging from 6 to 14). Methods Eighty children with 160 eyes of low myopia in the outpatient department of ophthalmology of our hospital were chose from July 2010 to April 2013, then the patients were equally divided into four groups:the control, esculin-digitalisglycosides eye drops,tropicamide,and tropicamide and esculin-digitalisglycosides combination groups. The naked vision and myopia correction of each group were compared before and after treatment for two weeks, and the treatment effects were compared among four groups. Results (1) The results showed there were no significant changes before and after treatment in the control, esculin-digitalisglycosides eye drops and tropicamide groups ( >0.05); (2) On the contrast, the tropicamide and esculin-digitalisglycosides combination have significantly improved naked vision and myopia correction after 2 weeks ( <0.05);(3) Prior to the treatment,there was no significant difference among four groups (<0.05);(4) After treatment,group under tropicamide and esculin- digitalisglycosides combined was statistical different from the other groups treated with other three eye drops ( <0.05);However, there was no statistical difference among groups in these three eyedrops. Conclusion Esculin-digitalisglycosides combined with tropicamide was the most effective treatment for low myopia of children.

Objective To evaluate the value and the significance of 16-slice spiral computed tomography angiography(MSCTA) in identifying the vertebral artery course variation.Methods MSCTA data in 22 patients with anomalous path of vertebral artery were retrospectively analyzed,13 of them had undergone DSA examinations,and 5 of them had also undergone MRI examinations.Multi-planar reconstruction(MPR),curved MPR,thin-slab maximum intensity projection(MIP),volume rendering(VR) or slab VR(cut-plane VR) were used to assess the anomalous path of the vertebral artery and its relationship with the adjacent large vessels.Virtual endoscopy(VE) was used to evaluate the orifice of the vertebral arteries and its relationship with the aortic arch and the ostia of the adjacent large vessels.Different views of vertebral artery were analyzed on DSA.MSCTA and DSA images were analyzed in blinded fashion for the accuracy of anomalous vertebral artery path and origin detection.Results were compared in a secondary consensus evaluation.Results The anomalous path of the vertebral artery in all 22 patients were correctly displayed on MSCTA,7 of them had anomalous origin of vertebral artery,the anomalous of ostia showed clearly.The vertebral arteriae with course variation were all going up at the front of the foramen transversarium of C6,then bended into the C5,C4 or C3 foramen transversarium.DSA could only be detected the anomalous origin in 13 patients undergone DAS.5 patients indirectly showed having course variation of vertebral artery by MRI.Conclusion MSCTA is superior to DSA in showing the anomalous path and orifice of vertebral artery,it should be considered as a non-invasive imaging tool for these diseases.

OBJECTIVE To investigate the use of antibiotics in our Outpatient Department during 2006-2007,and to analyze and evaluate the trend and rationality.METHODS The data about consumption quantity and consumption sum of antibiotics in our Outpatient Department during 2006-2007 were retrieved by means of computer management software in the department of pharmacy for the analysis of the clinical use of antibiotics using the method of defined daily dose(DDD).RESULTS Fluoro quinolones,cephalosporins and plant antibiotics have dominated the first places among the antibiotics used in our hospital,and the consumption of cephalosporins kept increasing year by year.CONCLUSIONS The use of antibiotics in our hospital should be further standardized to ensure the drug using safe,efficient and economic in our hospital.