1.Effects of adriamycin on cultured mouse podocytes VEGF expressions and the potential protective effects of dexamethasone.
Xiao-zhong LI ; Hai-tao YUAN ; Xue-guang ZHANG
Chinese Journal of Pediatrics 2003;41(2):146-146
Animals
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Antibiotics, Antineoplastic
;
pharmacology
;
Blotting, Northern
;
Cells, Cultured
;
Dexamethasone
;
pharmacology
;
Doxorubicin
;
pharmacology
;
Enzyme-Linked Immunosorbent Assay
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Gene Expression Regulation
;
drug effects
;
Glucocorticoids
;
pharmacology
;
Kidney Glomerulus
;
cytology
;
drug effects
;
metabolism
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Mice
;
RNA, Messenger
;
drug effects
;
genetics
;
metabolism
;
Vascular Endothelial Growth Factor A
;
genetics
;
metabolism
3.Meningeal hemangiopericytoma: a clinicopathologic study of 17 cases.
Hai-Bo WU ; Hai-Yan WENG ; Min DING ; Ping GU ; Xiao-Qiu WANG ; Wen ZHONG
Chinese Journal of Pathology 2011;40(4):251-252
Adult
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Aged
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Antigens, CD34
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metabolism
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Chondrosarcoma, Mesenchymal
;
metabolism
;
pathology
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Diagnosis, Differential
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Female
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Follow-Up Studies
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Hemangiopericytoma
;
metabolism
;
pathology
;
radiotherapy
;
surgery
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Humans
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Male
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Meningeal Neoplasms
;
metabolism
;
pathology
;
radiotherapy
;
surgery
;
Meningioma
;
metabolism
;
pathology
;
Middle Aged
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Neoplasm Recurrence, Local
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Proto-Oncogene Proteins c-bcl-2
;
metabolism
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Solitary Fibrous Tumors
;
metabolism
;
pathology
;
Vimentin
;
metabolism
;
Young Adult
4.Relation Between Hemoglobin and Blood Pressure
Hai-Lan ZHONG ; Xin-Zheng LU ; Xiu-Mei CHEN ; Xiao-Hui YANG ; Hai-Feng ZHANG ; Ke-Jiang CAO ; Jun HUANG ;
Chinese Journal of Hypertension 2006;0(12):-
Objective To study the relationship between peripheral blood hemoglobin (HB) and blood pres- sure.Methods We performed a cross-sectional analysis in 1153 subjects aged 29-83 years.Waist circumfer- ence,HB,blood pressure,high-density lipoprotein cholesterol(HDL-C),low-density lipoprotein cholesterol(LDL- C),triglycerides (TG),total cholesterol (TC) were determined.Results ①With the increasing of blood pres- sure,HB had a clearly increasing trend (HB,normotensive:137.5?14.7 vs prehypertension:143.4?14.4 vs hy- pertension:144.3?13.8 g/L,P
5.Determination of 27 elements in Maca nationality's medicine by microwave digestion ICP-MS.
Gui-fang YU ; Hai-jie ZHONG ; Jun-hua HU ; Jing WANG ; Wen-zhe HUANG ; Zhen-zhong WANG ; Wei XIAO
China Journal of Chinese Materia Medica 2015;40(23):4545-4551
An analysis method has been established to test 27 elements (Li, Be, B, Mg, Al, Sc, Ti, V, Cr, Mn, Fe, Co, Ni, Cu, Zn, Ga, As, Sr, Mo, Cd, Sn, Sb, Ba, La, Hg, Pb, Bi) in Maca nationality's medicine with microwave digestion-ICP-MS. Sample solutions were analyzed by ICP-MS after microwave digestion, and the contents of elements were calculated according to their calibration curves, and internal standard method was adopted to reduce matrix effect and other interference effects. The experimental results showed that the linear relations of all the elements were very good; the correlation coefficient (r) was 0.9994-1.0000 (Hg was 0.9982) ; the limits of detection were 0.003-2.662 microg x L(-1); the relative standard deviations for all elements of reproducibility were lower than 5% (except the individual elements); the recovery rate were 78.5%-123.7% with RSD lower than 5% ( except the individual elements). The analytical results of standard material showed acceptable agreement with the certified values. This method was applicable to determinate the contents of multi-elements in Maca which had a high sensitivity, good specificity and good repeatability, and provide basis for the quality control of Maca.
Lepidium
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chemistry
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Mass Spectrometry
;
methods
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Microwaves
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Reproducibility of Results
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Trace Elements
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chemistry
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isolation & purification
6.Bilateral congenital choanal atresia with supernumerary nostril and osteoma of ethmoid sinus in a case.
Xue-Hai WANG ; Xue-Zhong LI ; Xiao-Lan CAI
Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2008;43(4):309-310
Adult
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Choanal Atresia
;
complications
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Ethmoid Sinus
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Humans
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Male
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Nose
;
abnormalities
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Osteoma
;
complications
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Paranasal Sinus Neoplasms
;
complications
7.5-HT(1A) receptors are involved in the modulation of respiratory rhythmical discharge activity in the medulla oblongata slice preparation of neonatal rats.
Zheng QIN ; Zhong-Hai WU ; Xiao-Feng WANG
Acta Physiologica Sinica 2007;59(3):293-298
The present study was carried out to determine the role of 5-HT(1A) receptors in the generation and modulation of basic respiratory rhythm. Neonatal (aged 0-3 d) Sprague-Dawley rats of either sex were used. The medulla oblongata slice was prepared and the surgical procedure was performed in the modified Kreb's solution (MKS) with continuous carbogen (95% O2 and 5% CO2), and ended in 3 min. In cold MKS, a 600-700 microm single transverse slice was cut, which was rostral to the edge of area postrema and retained the hypoglossal nerve roots and some parts of the ventral respiratory group. The preparation was quickly transferred to a recording chamber and continuously perfused with carbogen-saturated MKS at a rate of 4-6 mL/min at 27-29 degrees C. Glass adsorb-electrodes containing Ag-AgCl needle were attached to the ventral roots of the hypoglossal nerve. Respiratory rhythmical discharge activity (RRDA) of the rootlets of hypoglossal nerve was recorded. Ten medulla oblongata slice preparations were divided into two groups. In group I, 5-HT(1A) receptor specific agonist (+/-)-8-hydroxy-2-(di-N-propylamino)tetralin hydrobromide (8-OHDPAT, 20 micromol/L) was added into the perfusion solution for 10 min first, after washing out, the 5-HT(1A) antagonist [4-iodo-N-[2-[4-methoxyphenyl]-1-piperazinyl]ethyl]-N-2-pyridynyl-benzamide hydrochloride] (PMPPI, 10 micromol/L) was applied to the perfusion solution for 10 min. In group II, after application of 8-OHDPAT for 10 min, additional PMPPI was added into the perfusion solution for 10 min. The discharges of the rootlets of hypoglossal nerve were recorded. Signals were amplified and band-pass filtered (100-3.3 kHz). Data were sampled (1-10 kHz) and stored in the computer via BL-420 biological signal processing system. Our results showed that 8-OHDPAT increased the respiratory cycle (RC) and expiratory time (TE) as well as reduced the integral amplitude (IA), but the changes of the inspiratory time (TI) were not statistically significant. PMPPI induced a significant decrease in RC, TE and TI, but the changes of IA were not statistically significant. The effect of 8-OHDPAT on the respiratory rhythm was partially reversed by additional application of PMPPI. Taken together with previous results, 5-HT(1A) receptors may play an important role in the modulation of RRDA in the medulla oblongata slice preparation of neonatal rats.
8-Hydroxy-2-(di-n-propylamino)tetralin
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pharmacology
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Animals
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Animals, Newborn
;
Medulla Oblongata
;
physiology
;
Piperazines
;
pharmacology
;
Pyridines
;
pharmacology
;
Rats
;
Rats, Sprague-Dawley
;
Receptor, Serotonin, 5-HT1A
;
physiology
;
Respiration
8.Determination of five kinds of trace ginkgolic acids in diterpene ginkgolides meglumine injection materials by LC-MS/MS.
Hai-hong SI ; Yan-jing LI ; Jia XUE ; Wen-zhe HUANG ; Zhen-zhong WANG ; Wei XIAO
China Journal of Chinese Materia Medica 2015;40(14):2832-2836
To develop a LC-MS/MS method for the determination of five kinds of trace ginkgolic acids in diterpene ginkgolides meglumine injection materials, the column was Agilent ZORBAX Eclipse plus C18 (3.0 mm x 50 mm, 1.8 µm), and the mobile phase consisted of methanol-water (containing 0.2% formic acid) (95:5) at a flow rate of 0.5 mL · min(-1). The multiple reaction ion monitoring (MRM) with an ESI interface in the negative ion mode was selected. The results showed that the linear ranges of five kinds of ginkgolic acids were in the range of 0.2-36.0 µg · L(-1) (r ≥ 0.999 5). The lowest limit of quantification (LOQ) of ginkgo acid C13: 0, C15:1, C17:2, C15:0 and C17:1 were 0.18, 0.18, 0.21, 0.10 and 0.20 µg · L(-1), respectively. The average recovery was between 73.28% and 87.56%, and the average content of total ginkgolic acids in three batches of samples was in the range of 0.023-0.028 µg · g(-1), which was much lower than 2 µg · g(-1) prescribed in drug registration standards. This method is simple and rapid with high sensitivity, which can be used for the determination of five kinds of trace ginkgolic acids in diterpene ginkgolides meglumine injection materials.
Chromatography, Liquid
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methods
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Ginkgolides
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analysis
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Injections
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Limit of Detection
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Salicylates
;
analysis
;
Tandem Mass Spectrometry
;
methods
9.ISOLATION OF FOREIGN NEURONS IN CORNU ANTERIUS MEDULLAE SPINALIS AFTER ARTIFICAL SOMATIC-AUTONOMIC NERVE ANASTOMOSIS OF RATS AND STUDY OF EXPRESSION CHANGES OF AGRIN
Bo HAI ; Zhaohui CHEN ; Yu LIU ; Wei LI ; Wei ZHONG ; Chuanguo XIAO
Acta Anatomica Sinica 2002;0(05):-
Objective To study the changes of agrin expression in spinal cord motor neurons after somatic-autonomic ventral root(VR) anastomosis and somatic-somatic VR anastomosis,and the factors related to the synapse formation of foreign nerve regeneration. Methods Artifical somatic-automomic reflex pathway and somatic nerve self-anastomosis were established in rats.Retrograde tracing was carried out to label the motor neurons,and the neurons were isolated by flow cytometry.Real-time PCR was used to investigate the agrin mRNA expression of the neurons.Anterograde tracing and immunofluorescence technique were employed to label the new synapses of pelvic ganglia(PG). Results After 4 months of surgery,the somatic nerve can regenerate into PG successfully and form new synapses observed under laser scanning confocal microscope.The neurons involved in foreign nerve regeneration can be isolated successfully by retrograde tracing and flow cytometry.The expression of agrin in L_4-L_6 VRs anastomosis group was lower than that of the control group of L_4-L_4 VR self-anastomosis(P
10.Epidemiological analysis of human brucellosis in Ningxia from 2004 to 2009
Xiao-jia, SONG ; Feng, JIN ; Xing-zhong, HU ; Tian-bo, MA ; Hai-hong, WANG ; Li, LI
Chinese Journal of Endemiology 2011;30(1):92-95
Objective To learn the prevalence and trends of Brucella disease in Ningxia, in order to provide scientific basis for effective control of the disease. Methods Data of Brucella cases reported through city network from 2004 to 2009 in Yinchuan city, Shizuishan city, Wuzhong city, Guyuan city and Zhongwei city of Ningxia were reviewed and retrospectively analyzed. The data included demographic characteristics, treatment conditions and medical history so on related information. Analytical indicators include reported incidence;with patients' gender, age, regional distribution, urban and rural distribution;become chronic and associated factors;distribution of the cases reporting unit and so on. Results From 2004 to 2009, Ningxia had reported 349 cases of Brucellosis, no deaths, the annual incidence rates reported were 0.017/10 million, 0.543/10 million, 0.151/101 (295/54);The proportion of 34- to 40-year-old age group was higher than other age groups(27.5%, 96/349);Occupational distribution of patients was mainly farmers and herdsmen(70.2% ,245/349), in regional distribution of the patients, the highest percentage was Wuzhong city(61.9%,216/349), followed by Yinchuan city(22.9%,80/349);The proportion of patients in rural areas(97.4% ,340/349) was higher than urban(2.6% ,9/349);the proportion of patients converted to chronic was 11.2% (39/349). With age, the chance of patients converted to chronic was in a decreasing trend(odds ratio was 0.966);cases reported by Centre for Disease Control and Prevention accounted for 74.8%(261/349), by hospital accounted for 25.2%(88/349). Conclusions The reported incidence of Brucellosis in Ningxia is in a rapid upward trend year by year, the patients is mainly young men, the rate of converted to chronic is higher and the ability of hospital in founding and reporting of the cases is weaker.Comprehensive measures should be taken to increase funding, strengthen monitoring, and continuously improve the level of awareness and diagnosis of medical personnel for further strengthen the prevention and control of Brucellosis.