Arteriosclerosis Obliterans ; surgery ; Graft Occlusion, Vascular ; therapy ; Humans ; Lower Extremity ; blood supply ; Stents

OBJECTIVE: To study the expression of Endophilin II and VEGF-C in human Laryngeal cancer and its relationship with the clinical treatment effect. METHOD: The expression and distribution of Endophilin II and VEGF-C protein were checked in 47 patients with laryngeal carcinoma in our hospital in 2006-09-2009-10 and 23 samples of normal tissues around the cancer by immunohistochemical staining SP method. After an appropriate surgical treatment, patients accepted a 5-year followup to evaluate their recurrence situation and 5-year survival rate. RESULT: Immunohistochemistry showed that Endophilin II protein was strongly positive expressed in normal tissues around the cancer (positive rate 82.61%). And the expression level was dependent on the lymph metastasis, T stage and lymph invasion (P < 0.05). Patients with positive Endophilin II expression had a higher survival rate and better recurrence situation. Expression of VEGF-C was significantly higher in laryngeal carcinoma than in normal tissue around the cancer (positive rate 51.06% and 13.04%). the expression level was dependent on the lymph metastasis and lymph invasion (P < 0.05), and negatively correlated with recurrence and 5-year survival rate. CONCLUSION Endophilin II and VEGF-C may involve in laryngeal cancer, and the expression of Endophilin II and VEGF-C has a positive correlation with pathological features and lymph metastasis, and both are related with recurrence and 5-year survival rate. So these two proteins are likely to be a potential target for diagnosis and treatment of laryngeal cancer.
Humans ; Immunohistochemistry ; Intracellular Signaling Peptides and Proteins ; metabolism ; Laryngeal Neoplasms ; metabolism ; pathology ; Lymphatic Metastasis ; Neoplasm Recurrence, Local ; Survival Rate ; Vascular Endothelial Growth Factor C ; metabolism

Objective To introduce a new flap of cutaneous branches of posterior tibial artery on the part of superior medial malleolus to repair soft tissues defect on the feet and the ankles Methods From October 1997 to December 2001,a flap with the pedicle called cutaneous branches of posterior tibial artery on the part of superior medial malleolus were used to repair 11 cases which suffered skin and soft tissue defect on the feet and the ankles due to various trauma Results The flaps were survived completely on 10 cases and the flaps distal had about 2 cm necrosis on 1 case,the size of the flap ranged from 28 cn?11 cm to 8 cm?6 cm,all of the 11 cases were evaluated as satisfactory after 6 months to 2 years follow-up Conclusions The new procedure can repair mostly soft tissue defect on the foot and the ankle and does not sacrifice the major arteries. The flap is easy to be performed,and it is an safety and ideal method.

Objective To explore the feasibility and indications of non-operative management for blunt liver injury.Methods The clinical data of 109 patients with blunt liver injury treated in recent 5 years were reviewed retrospectively.Results Among 109 cases, 35 were treated with nonoperation and 33(94.3 %) were cured; there were 3 cases(9.1 %) with complications among the 33 cases who were cured. The mean amount of blood transfusion, hospitalization days and expenses were (2.5?0.8)U,(13.2?1.3)d and (5 250?335) yuan, respectively. Seventy-four cases were treated with operation and 68(91.2 %)were cured, there were 14 cases (20.6 %) with complications among the 68 cases who were cured. The mean amount of blood transfusion,number of hospitalization days and expenses were (8.4?1.1)U,(15.4?0.9)d and (13 550?805) yuan,respectively.The mean amount of blood transfusion and hospitalization expenses of nonoperative group were lower than those of operative group (P

Objective To evaluate the actual displacement of a femoral neck fracture following close reduction under the C-arm X-ray perspective.Methods From June, 2006 to June, 2009, we managed 12 patients with femoral neck fracture by open reduction and internal fixation.They were 8 males and 4 females, aged from 23 to 65 (average, 42.3) years old.We tried close reduction of their fractures under the C-arm X-ray perspective before a temporary fixation.Next we performed open reduction, readjusted the fracture ends, and placed internal fixation.The actual displacement of the fracture measured after open reduction was carefully compared with the close reduction under the X-ray perspective.Results A substantial displacement was found after open reduction in 7 patients who had achieved anatomic reduction under the X-ray perspective.In the other 5 patients who presented with a slight displacement after close reduction under X-ray perspective, the actual displacements were larger than the X-ray observations but completely reduced after open reduction.The actual measurements after open reduction were significantly larger (3.21 ± 0.96 mm on average) in the anteroposterior displacement ( t = 11.540, P = 0.000), but insignificantly larger (0.58 ±0.93 mm on average) in the superior-inferior displacement ( t = 2.184, P = 0.346) than those after close reduction.Conclusions In general, anatomic reduction can not be achieved for a femoral neck fracture under the C-arm X-ray perspective.Consequently, open reduction may be better than close reduction in the treatment of femoral neck fracture in that improper reduction can block blood supply to the femoral neck and dissection of the capsula articularis coxae can improve blood supply as well as help decompression.

Objective To understand the status of the sanitary quality in some areas of countryside in Guangxi. Methods The investigation on the type of water source and the water supply was carried out in 9 counties.10 sampling points were established in each observed county.91 water samples were collected totally for the measurement of water quality ,then their sanitary quality was assessed based on the Criteria for Implementing the Hygienic Standards for Drinking Water in Countryside. Results In the observed 9 counties ,15.33% of total population drank water supplied with surface water,and 84.67% with ground water source. Drinking water from centralized water supply system was supplied to 43.16% of total population.In the centralized water supply system,the finished water completely treated by sedimentation and filtration was supplied to 10.57% of the total population,the disinfected finished water was only supplied to 2.96% of the total population,the raw water was directly supplied to 68.66% of the tap water-supplied population without any treatment.The completely-qualified rate of water samples was 20.88% for total 91 water samples,14.81% for surface water samples and 23.44% for ground water samples respectively.The qualified rate of bacterial indexes was 32.61% for water samples collected from centralized water supply system and 20.00% for those from decentralized water supply system. Conclusion Most of the peasants in the investigated 9 counties hadn't been supplied by safe and health drinking water.Contamination of drinking water by microorganisms was the main sanitary problem in water supply in countryside of Guangxi.

Acute Disease ; Adult ; Humans ; Male ; Occupational Diseases ; diagnosis ; therapy ; Phosphines ; poisoning ; Poisoning ; diagnosis ; therapy

Objective To investigate the status of the cognition ,attitude and behavior related to hepatitis B among the college students in the business school ,and to provide the reference for making a reasonable and effective measures of prevention and health education .Methods A self‐designed questionnaire was used to surveyed 548 college students .Results The total awareness rate a‐bout the core information of hepatitis B prevention and treatment among the students was 81 .81% ,and rate of awareness of each hepatitis B question was between 54 .56% and 95 .44% .The total awareness rate of girls(82 .45% )was higher than boys(80 .69% ) (χ2 =5 .536 ,P=0 .019) .The total awareness rate of students in grade 3 and 4 (83 .13% )was higher than that of students in grade 1(79 .44% )(χ2 =17 .247 ,P=0 .000) .78 .83% student treated hepatitis B patients with the right mental attitude and students ac‐quired knowledge mainly from TV‐broadcast(75 .55% ) .Conclusion The college should strengthen health education on hepatitis B to improve students′awareness of hepatitis B ,eliminate hepatitis B discrimination and to improve the students′consciousness of prevention and treatment .

Objective To study the blood_flow_activating and blood_stasis_removi ng actions of Qushang Tablet (QT) and its safety. Methods Thirty rats were randomly allocated to Group A (normal control), Group B (models control) and G r oup C (treated with QT). Rat models with traumatic blood_stasis syndrome were in duc ed by the bruise of falling iron hammer. Effects of QT on blood rheology of rat model were studied. Acute and chronic toxic effect of QT was also studied. Res ults QT had obvious effect on blood rheology of rat model and could improve th e concentration, viscosity, aggregation and coagulability of blood. No acute and chronic toxic effect of QT was found. Conclusion QT exerts obvious blood_fl ow_activating and blood_stasis_removing actions and is safe to be applied in cli nic.

BACKGROUND: In the field of intervertebral disc tissue engineering, seed cells primarily consist of nucleus pulposus cells (NPCs) and mesenchymal stem cells (MSCs). NPCs have been known to have several shortcomings: limited source, inconvenient collection, poor proliferative capacity, and difficult in vitro culture.OBJECTIVE: To investigate the proliferation of MSCs after co-culture with NPCs in alginate beads-simulated three-dimensional environment.DESIGN, TIME AND SETTING: A cell observation was performed at the Laboratory of Orthopedics, Tongji Hospital, Tongji Medical College of Huazhong University of Science and Technology between December 2007 and October 2008.MATERIALS: Six healthy New Zealand rabbits of 4 months old were provided by the Laboratory Animal Center, Tongji Medical College of Huazhong University of Science and Technology and were used for the present study.METHODS: Rabbit MSCs were isolated and purified using density gradient centrifugation and adherence methods. Rabbit NPCs were isolated and purified using collagenase Ⅱ digestion and adherence methods. Following liposome-mediated green fluorescent protein tranfection and G418 screening, MSCs of passage 3 were cultured either alone (control group) or with NPCs at a ratio of 1:1 (co-culture group) in alginate beads. After 14 days of culture, alginate beads were dissolved and MSCs were collected by flow cytometry sorting.MAIN OUTCOME MEASURES: The expression of collagen Ⅱ and aggrecan in MSCs was detected by RT-PCR and Western blot techniques.RESULTS: mRNA and protein expression of collagen Ⅱ and aggrecan was observed in the co-culture group, but not in the control group, after 14 days of culture.CONCLUSION: MSC differentiation towards nucleus pulposus-like cells can be induced by co-culture with NPCs in a three-dimensional environment.