80 male Wistar mice were randomly divided into 8 groups. The effects of the big black Ant preparations (alias Xuanju ) obtained in different ways and their compound preparations in different dosages on the amount of RNA and DNA in the testes of mice were observed. After administration of the preparations for 21 days, the mice were decapitated and the testes were excised and weighed. The RNA and DNA in the testes were isolated andpurified with the method of SDS- phenol and their amounts were measured with UV-VIS spectrophotometry. The results showed that the mean testes weight in the experimental groups was significantly higher than that in the control group (P

Deep venous thrombosis in the lower extremities is a very common disease clinically and can greatly affect people in their work and life without proper treatment promptly.The author reviewed the etiology,diagnosis,preventive measures and the synthetic therapies for dealing the deep venous thrombosis in the lower extremities,especially concerning the interventional radiology.

Based on ESI web of science,the article analyzed papers published by three Medical Schools from 2001 to 2012 in terms of volume of publication,cited times,discipline distribution,au thors and cooperation,and determined each factor's impact on research output and performance.Sug gestions and advice were given to enhance the overall basic medical science research level.

Humans ; Occupational Diseases ; prevention & control ; Occupational Health Services ; organization & administration ; Risk Assessment ; Workplace

BACKGROUND: A left-to-right shunting animal model is needed to study chronic pulmonary vascular remodeling of congenital heart attachment disease in adult.OBJECTIVE: To observe the characteristics of chronic pulmonary arterial hypertension in rat models subjected to left-to-right shunting.DESIGN, TIME AND SETTING: Randomized controlled animal modeling observation was performed in Experimental Animal Center, Xinqiao Hospital, the Third Military Medical University of Chinese PLA from April 2008 to April 2009.MATERIALS: A total of 80 male inbred line healthy SD rats of specified-pathogens free class, 4 weeks old, weighing 95-110 g, were randomly assigned into control group (n=40) and shunt operation group (n=40). A shrinking polyethylene blood vessel connector (proximal part inner diameter was 0.6 mm and outer diameter was 0.8 mm, while distal part's was 0.8 mm and 1.0 mm, respectively, 1.6 mm long), was applied.METHODS: Shunt operation group underwent left-to-right shunting through right common carotid artery and external jugular vein connected by shrinking polyethylene blood vessel connector. Control group underwent sham operation. At 4, 8, 12 and 16 weeks postoperation, ten models were determined with a series in procedures: hemodynamics detection, pathological sample preparation, hematoxylin-eosin staining, imaging analysis and so on.MAIN OUTCOME MEASURES: Changes in right ventricular systolic pressure, right ventricular hypertrophy index of right ventricle to left ventricle plus interventricular septum, and relative medial wall thickness in moderate sized pulmonary arteries in rats of shunt group and control group.RESULTS: All animal were survived. The pulmonary blood flow in shunt group was significantly higher than control groups (P<0.01). Compared with control group, right ventricular systolic pressure elevated obviously and right ventricular hypertrophy index were significantly higher in shunt group at 12 and 16 weeks (P<0.01). Compared with control group, the relative medial wall thickness in pulmonary arteries also elevated obviously in shunt group at 12 and 16 weeks (P<0.01).CONCLUSION: Left-to-right continuous low-flow shunt in rats can induced right ventricle accommodated with the change of right ventricular systolic pressure and promote chronic pulmonary arterial remodeling continuously. The procedure of establishing left-to-right shunting models with common carotid artery and external jugular vein connection, is a stable tool for studying chronic pulmonary arterial hypertension induced by low flow.

Objective To compare the efficiency of repairing perimembranous ventricular septal defect (VSD) with continuous stitching to that with interrupted stitching. Methods Of the 158 patients with perimembranous VSD ( 54% males) ,102 ( 65% ) patients underwent the repairing of continuous stitching (continuous group) ,and 56 patients underwent interrupted stitching (interrupted group). All the surgeries were performed under hypothermic cardiopulmonary bypass. Results The cardiopulmonary bypass time and aortic cross-clamp times were (31 ±14) mins and (18 ±12) mins in the continuous group, which was significantly less that those of (42 ± 16) mins and (25 ±11) mins in the interrupted group (t =4.49 and 3. 61 .respectively ,P <0. 05) . No case was died. The main complications involved temporary M atrioventricular block (AVB) of two cases (1 case in each group),which disappeared after 1 week. There were two remnants remaining leak (n =2) which was less than 3 mm and automatically cured after 3-6 months. Complete right bundle branch block(CRBBB) occurred in 3 cases in the continuous group and 6 cases in the interrupted group,of which 3 disappeared after 1 month and 2 disappeared after 12 months. The outcome was good in all cases after 1-3 years follow up. Conclusions Continuous stitching showed a shorter cardiopulmonary bypass and aortic cross-clamp times, and reduced the CRBBB.

Aorta, Abdominal ; injuries ; Blood Loss, Surgical ; prevention & control ; Hemorrhage ; therapy ; Hemostasis, Surgical ; methods ; Humans ; Mesenteric Veins ; injuries ; Splenic Diseases ; therapy ; Vena Cava, Inferior ; injuries

A SnO2-CuO composite nanofiber was prepared by the coaxial electrospinning method. A new thin-film-type hydrogen sulfide gas sensor was designed by coating SnO2-CuO composite nanofibers onto an alumina ceramic tube with Au electrodes by dip-coating method. The crystalline phase and microstructure of SnO2-CuO composite nanofibers were characterized using X-ray diffraction ( XRD ) and scanning electron microscope ( SEM) . The influence of chemical composition and thickness of sensitive film on the sensitive mechanism and electrochemical characteristic of SnO2-CuO nanofibers were analyzed. The characteristic tests of hydrogen sulfide sensor including sensitive performance, temperature, relative humidity, dynamic response, interference and stability were carried out by WS-30A type multifunction analyzer in gas sensor test system. The results demonstrated that, when the operating temperature was 25℃ and hydrogen sulfide gas concentration increased from 10 to 60 mg/L, the hydrogen sulfide sensor based on C50 composite nanofibers with 70 nm sensitive film thickness had the best linearity (92. 3%) and sensitivity (98. 2%). Besides, its highest response values and relatively humidity level were 1080 and 95%, respectively, and its dynamic response time and recover time were 4 s and 12 s, respectively. This sensor showed good anti-disturbance to the gases, such as CO, NO2 , SO2 , NH3 , CO2 , CH4 and H2 . The response value of the sensor was attenuated about 9. 2% when it was applied continually in the mine about 12 months, and its normal response time was 10. 9 months.

Currently,the diagnosis of malignant pheochromocytoma still faces a big challenge.Neither biochemical nor histological features can be reliably used to distinguish malignancy from benign tumors.Numerous efforts have been applied to identify novel molecular markers to predict malignancy.

Objective To express and preliminary identify scavenger receptor B2 (SCARB2)protein.Methods SCARB2 cDNA was amplified by reverse transcription-polymerase chain reaction (RT-PCR)from mRNA extracted from RD cells,and cloned into pMD19-T vector.Expression vector pET28a(+)/SCARB2 was constructed,and SCARB2 protein was expressed in prokaryotic ex-pression system.Obtained recombinant proteins were identified by detection of Western blot using His labeled monoclonal antibody.Results Recombinant SCARB2 proteins,expressed by induction of isopropy-β-D-thiogalactoside (IPTG),were success-fully purified and specifically recognized by His labeled monoclonal antibody.Conclusion SCARB2 proteins could be expressed and identified successfully,which could provide reference for researching mechanism of enterovirus type 71 (EV71)and scavenger re-ceptor protein,and for preparation of SCARB2 monoclonal antibodies.