Objective To observe the expression of angiotensin Ⅱ type 2 receptor (AT2R) and coronary arterial remodeling in ischemia-reperfusion(I-R) injury rat after the renin -angiotensin blocker valsartan pretreatment .Methods 123 rats were random-ly divided into 3 groups :sham -operation group(sham) ,control group and valsartan group(ARB) .The control group and the sham group received the gavage of normal saline and the ARB group received valsartan gavage 10 mg/(kg · d) for 4 weeks before surger-y .The I-R injury rat model was established by thoracotomy for ligating the left anterior descending (LAD) coronary artery and re-moving the ligation after 30 min .The sham group was performed the thoracotomy without ligation .At the 4 timepoints of postoper-ative 3 ,7 ,14 ,28 d ,the left ventricular diastolic and systolic pressures were measured ,then the rats were killed for collecting the rat heart sample .The section was stained by sirius red .The collagen deposition in coronary arterial remodeling and coronary arterial pe-ripheral area ,and the dynamic change of location and expression of AT2R in the coronary artery were observed by the immunohisto-chemical streptavidinbiotin peroxidase complex (SABC) .Results The immunohistochemical analysis revealed that AT 2R was local-ized in the adventitia of coronary arteries with the radial distribution ,showing the higher density especially in large coronary arterial peripheral area ,the partial expression existed in the coronary arterai intima .The expression of AT2R was transient ,which on 7 d af-ter I-R operation in the control group reached the peak value ,while the expression peak value of AT2R in the valsartan group was higher and earlier than that in the control group .The heart diastolic function on 3 d after I-R operation was impaired ;the left ven-tricular end diastolic presure (LVEDP) in the valsartan group was significantly lower than that in the control group .The collagen deposition of coronary peripheral mesenchyma on postoperative 28 d in the valsartan group was significantly lower than that in the control group;the coronary peripheral collagen deposition in the control group was gradually increased with the time progression , which on postoperative 14 ,28 d was significantly higher than that in the sham group .Conclusion Valsartan could inhibit the coronary arterial remodeling for protecting the heart function possible by the transient high AT 2R expression after myocardial I-R injury .

BACKGROUND:Umbilical cord mesenchymal stem cels are from the umbilical cord of newly born individuals and have no ethical issues, and therefore are promising candidates for seeded cels as a substitute for cel transplantation and regenerative medicine. OBJECTIVE:To investigate the effects of serum from liver injury rats on induced differentiation of human umbilical cord mesenchymal stem cels into hepatocyte-like cels and provide experimental evidence for use of human umbilical cord mesenchymal stem cels in the treatment of patients with end-stage liver disease in the clinic. METHODS: Rat models of acute liver injury were established by intraperitoneal injection of 10% carbon tetrachloride. Rats in the control group were intraperitonealy administered the same amount of soybean oil. Forty-eight hours after modeling, abdominal aorta blood was taken for serum preparation. Passage 3 human umbilical cord mesenchymal stem cels were cultured with 20% serum from liver injury rats and 20% fetal bovine serum. Morphology of human umbilical cord mesenchymal stem cels was observed before and after culture. Levels ofα-fetoprotein and albumin in the supernatant were detected. RESULTS AND CONCLUSION:Cels exhibited shuttle-shaped appearance and grew in whirlpool-like manner at 1 day after culture with serum from liver injury rats, exhibited short shuttle-shaped appearance at 2 days, were oval-shaped at 3 days, and were round and an extremely smal number of cels were floated at 4 days. At 4 days after culture with serum from liver injury rats, level of albumin in the cel supernatant was significantly increased than that before induction and that in the control group (P< 0.001), and there was no significant difference in level of α-fetoprotein in the cel supernatant. These results suggest that serum of liver injury rats can induce differentiation of umbilical cord mesenchymal stem cels into hepatocyte-like cels.

The studies on environmental cadmium (Cd), lead (Pb), arsenic (As) pollution and health impact on exposure population in China were reviewed in the present paper. The related reports said the main pollution sources of serious environmental Cd, Pb and As pollution in China were mining and smelting of nonferrous metals, these pollutants were discharged into the environment and caused the pollution of water, atmosphere, soil and biological species and caused a series of acute and chronic diseases in the exposed people, but the relationship between malignant tumors and environmental cadmium, lead, arsenic pollution is not well understood up to now. The researches should focus on the assessment of environmental pollution and health impact through the effect identification, dose-effect assessment, exposure evaluation and description of risk characteristic.

Objective　In order to improve diagnosis of the disease,CT appearances of pulmonary sequestration were studied.Methods　10 cases were proved by surgery and pathology.Lung inner type was in 9 and lung exterior type in 1.All cases were examined with plain CT scans and 5 of them were taken thin slice CT scan with contrast enhancement.Results　10 cases located in the posterior segment of right or left lower lobes,3 of them were cystic,3 were solid,4 were cystic and solid.The emphysema around the lesions were showed in 7 cases.Abnormal arterial supply could be seen in 7 cases.Conclusion　CT not only can demonstrate lesions and changes of tissue surrounding lesions,but also can manifest abnormal supplying blood vessels well.CT plays important role to diagnose pulmonary sequestration.

AIM: To explore the oxidant-antioxidant status and levels of mononuclear cells (PBMCs) oxdative DNA damage in patients with type 2 diabetes mellitus (T2DM), and to value the effect of resveratrol on peripheral blood mononuclear cells oxidative DNA damage in T2DM patients in vitro. METHODS: The DNA damages in PBMCs of 39 T2DM patients with vascular complication, and 29 T2DM patients without complication were detected by single cell gel electrophoresis (the comet percentage). The levels of plasma total antioxidative capacity (TAC), the contents of plasma malondialdehyde (MDA) and plasma thiol group (SH) were measured. The effect of resveratrol on DNA damage of T2DM patients were investigated in vitro. In addition, 26 healthy subjects were studied as a control group. RESULTS: The comet percentage of PBMCs and the contents of plasma MDA in T2DM patients without complication were all significantly higher than that in healthy controls (P

To attach importance to practice teaching in clinical microbiological analysis,the overall planning,research-on-research and systematic training must be carried out.

Graduation Paper is a key to achieve the objective of cultivating undergraduates majoring in laboratory medicine.To improve the quality of graduation papers,the paper focuses on establishing a system of quality monitoring of graduation papers by students major in laboratory medicine and the difficulties of sustainable developments in graduation papers.

Objective To evaluate performance of the AVE‐766 automated urine sediment analyzer(AVE‐766) based on the ma‐chine vision for detecting erythrocytes(RBCs) ,leukocytes(WBCs) ,epithelial cells(ECs)and CASTs in urine specimen .Methods The within‐run variable coefficients(CVs) ,linearities and carryover rates for RBC ,WBC ,EC ,and CAST in urine specimens detected by using the AVE‐766 were analyzed ,the results of RBC ,WBC ,EC ,and CAST count in urine specimens detected by using AVE‐766 and Fast‐Read102 counting plate(Fast‐Read102) were compared .Results The within‐run CVs for RBC ,WBC ,EC ,and CAST detected by using AVE‐766 and Fast‐Read102 were increased by decreases of concentration of urine sediment .Good linearities (R2 >0 .97 ,P<0 .05) were observed for RBC(in the range of 60 -1 255/μL) ,WBC(in the range of 68 -2 718/μL) ,EC(in the range of 28-296/μL) and CAST(in the range of 5-86/μL) detected by using the AVE‐766 .The carryover rates for RBC ,WBC , EC ,and CAST detected by using AVE‐766 was 0 .9% or less .The values detected by using AVE‐766 were correlated well with those detected by using Fast‐Read102 for RBC ,WBC ,and EC in urine specimens(0 .67< r<0 .75) .However ,for CAST ,the values detected by using AVE‐766 were poorly correlated with those detected by using Fast‐Read102(r=0 .183) .There were statistically significant differences between manual and automated urinalysis for RBC ,WBC ,EC ,and CAST in urine specimens(P<0 .05) .Con‐clusion The AVE‐766 could not take over microscopic examination ,only is suitable for the first screening to detect RBC ,WBC , EC ,and CAST in urine specimen .

Objective To investigate the influence of newborns born to mother with systemic lupus erythematosus (SLE).Method The clinical data of SLE mothers and their infants bern in the obstetric and were admitted to the neonatal ward ward of the First Affiliated Hospital of Guangxi Medical University from July 2012 to March 2015 were studied retrospectively.The infants were divided into active SLEactivity group and stable SLE group.The incidence of preterm birth,small for gestational age (SGA),cardiac conduction block,anemia,and thrombocytopenia were compared between the two groups of SLE mothers.Result A total of 66 infants were included in SLE mothers,including 14 cases (21.2％) of preterm infants and 18 cases of SGA (27.3％).14 cases belonged to the active SLE group while 52 cases belonged to the SLE stable group.When comparing the 2 groups,there were no differences found on the rates of preterm infant and small for gestational age (P ＞ 0.05).The cardiac conduction block,anemia and thrombocytopenia happened separately in three cases of the active group,which had not seen in the SLE stable group.There was no statistically significant difference between the two groups (P ＞ 0.05).Of the 66 cases,2 were diagnosed with neonatal lupus erythematosus (NLE) with an incidence of 3％.Conclusion SLE mothers with an active disease 10 days before delivery did not significantly increase the incidence of preterm infants and SGA,but were at risk of NLE.

0.05. The reference range of seum TIBC( ?1.96s) was 51.3-76.3?mol/L(70 cases).Conclusion The method was stable,simple, sensitive, rapid and accurate, and suitable for the routine manul and automatic analysis of total iron-binding capacity in serum.