0.05).The levels of serum anti-MPO antibody in patients with lupus nephritis(LN)and purpuric glomerulonephritis had significantly increased(P

Objective:To explore the underlying molecular mechanisms of specific cyclooxygenase-2 inhibitor(Rofecoxib)and to evaluate its efficacy on MRL/lpr mice.Methods:12-week female MRL/lpr mice were given Rofecoxib (10mg?kg -1 ?d -1 )for 12 weeks.Suppressive effect of the drugs on serum and urinary levels of tumor necrosis factor-?(TNF-?),interleukin-6(IL-6) and urinary TXB 2 level were determined by using radio-immunological assay.Changes in proteinuria,serum creatinine,pathology of ltidney and the expression of CD14 in renal tissues were observed.Results:Compared with the ones in the control group,mice treated with Rofecoxib had lower levels of proteinuria( P

Objective :To study the significance of anticardiolipin antibodies (ACA) in patients with lupus nephritis( LN). Methods : We detected serum ACA in 32 cases of LN. The control group consisted of 28 healthy cases. We observed the relation between the levels of ACA and the levels of clinical biochemical index. Results :The positive rate of IgG- ACA, IgM- ACA IgA- ACA was 53. 1%, 40. 6% ,46. 9% in patients with LN. In patients with positive ACA, lower levels of hemoglobin , thrombocytopeinia and C3 were observed as well as elevated levels of serum IgG,24h- urine protein, antinuclear antibody titer and serum creatinine(P

0.05).Conclusion:LEF is an effective agent for treatment ofRA with better tolerability,and there are few adverse effects.

Objective To investigate the clinical effect of live combined bifidobacterium,lactobacillus and enterococcus powder (probiotics bifico) treatment in children with secondary diarrhea of pneumonia and analysis of related factors.Methods Three hundred and eighty-five pneumonia children were studied.The related factors of secondary diarrhea of pneumonia were screened.One hundred and twenty cases of children with secondary diarrhea of pneumonia were divided into observation group and control group by table of random digit with 60 cases each.The observation group was given the probiotics bifico combined with conventional treatment,the control group was given the conventional treatment.The clinical efficacy was evaluated treatment after 7 d.Results The effective rate of observation group was significantly higher than that of control group [96.7％(58/60) vs.61.7％(37/60),x2 =22.282,P＜ 0.01].Age of onset,hospitalization time,invasive procedures,combination therapy of antibiotics,therapy of hormone and therapy of probiotics bifico were related with the incidence of secondary diarrhea of pneumonia (P ＜ 0.01 or ＜ 0.05).Conclusions The younger children,long hospital stay,invasive procedures,combination therapy of antibiotics and therapy of hormone are the risk factors of secondary diarrhea of pneumonia.The probiotics bifico for treating the children with secondary diarrhea of pneumonia has exact clinical efficacy,and it is worthy of clinical application.

Objective To study the value of B-cell activating factor (BAFF) expression levels in the diagnosis and prog-nosis of children with idiopathic thrombocytopenic purpura. Methods Children with idiopathic thrombocytopenic purpura (ITP) admitted to our hospital from September 2011 to September 2013 were enrolled in observation group, and healthy check-up chil-dren during the same period were enrolled as controls. The serum levels of B-cell activating factor, platelet antibodies (PAIgG, PAIgM, PAIgA) and platelet count were detected, and curative effect in children with ITP was observed. Results The BAFF, PAIgG, PAIgM and PAIgA levels were higher and platelet count were less in both acute and chronic ITP children than those in normal controls. The difference was signiifcant (P<0.05). The results of linear regression analysis indicated that BAFF levels were positively correlated with PAIgG, PAIgM and PAIgA levels (β=1.87~2.25, P<0.05), and were negatively correlated with platelet count (β=-2.42, P<0.05). Meanwhile, BAFF levels were negatively correlated with curative effect in ITP children (β=-1.88, P<0.05). When the value of BAFF was 1.35, the sensitivity for predicting effective treatment was 0.84 and the speciifcity was 0.75. The area under the ROC curve was 0.85 (95%CI:0.73~0.98, P=0.000). Conclusions The serum BAFF expression was increased and closely related to the level of antiplatelet antibody, platelet count and treatment effect in ITP children, and therefore, was a good indicator for the diagnosis and prognosis of ITP.

Cerebellar Neoplasms ; genetics ; Chromosome Aberrations ; Genes, myc ; Genes, p16 ; Genes, p53 ; Humans ; Medulloblastoma ; genetics ; Mutation ; Neuroectodermal Tumors, Primitive ; genetics ; Supratentorial Neoplasms ; genetics

China ; Occupational Exposure ; prevention & control ; Risk Assessment ; Workplace

Objective To develop a sensitive,quick and convenient comet assay with Viciafaba root cells,so as to provide the reference for biomonitoring in situ.Methods The K_2CrO_7 and H_2O_2 were used as the positive control,the Viciafaba root cells isolated with mechanical and enzyme treatment respectively were exposed to the different concentrations of K_2CrO_7(1 nmol/L,10 nmol/L,100 nmol/L,1000 nmol/L)and H202(25 ? mol/L,50 ? mol/L,100 ?mol/L,200 ?mol/L).The effect of DNA damage was detected by using comet assay.Results The yield of Viciafaba root cells isolated from enzyme treatment were much higher than that from mechanical treatment.K_2CrO_7and H_2O_2 could cause DNA damage in Viciafaba root cells,there was significant difference between exposure group and control group(P

Isocitrate lyase (ICL) which plays a pivotal role in the glyoxylate cycle of mycobacterium tuberculosis(MTB) could be a potential target against mycobacterium tuberculosis.Deoxyribozyme(DRz) is a small single-strand,enzymatic DNA molecule.Assistant by sketch of secondary structure,appropriate and not appropriate target sequences of MTB ICL mRNA were predicted.Two DRzs were designed targeting above sequences respectively.To affirm feasibility of this kind of prediction,in vitro cleavage of MTB ICL mRNA segment by DRz-ICLcf and DRz-ICLcj were performed.The results showed that sketch of secondary structure could indeed predict potential target of DRz.This research will provide basis for developing DRz as a novel antimicrobial agent against MTB.