1.Titanium mesh combined with forearm free flap for maxillary defects is better to maintain swallowing and language function
Chinese Journal of Tissue Engineering Research 2015;19(30):4903-4907
BACKGROUND:Prosthetic appliance is often adopted for clinical maxilary repair, which can maintain swalowing and language function in patients with a certain extent, but if the defect is too large, the prosthesis has a poor fixed effect that is prone to inducing various adverse consequences. OBJECTIVE:To explore the maintenance effect of titanium mesh combined with forearm free flap on swalowing and language function in patients with maxilary defects. METHODS:A total of 35 patients with maxilary defects, 19 males and 16 females, aged 33-93 years, were enroled. Among them, 17 patients underwent maxilectomy and wound packing with iodoform gauzes, and then after 1 year, these patients were given prosthesis repair as control group; another 18 patients were subjected to titanium mesh combined with forearm free flap for repair of maxilary defects as observation group. Al the patients were folowed up for 24 months, and the swalowing and language functions were evaluated. RESULTS AND CONCLUSION:At 24 months after repair, al the patients were satisfied with facial appearance, and there were no adverse reactions related to repair materials. Scores on swalowing function, language function, pain fucntion, face function, motor function, entertainment function, masticatory function and oral health were significantly higher in the observation group than the control group (P < 0.05), and the score on the shoulder dysfunction was lower in the observation than the control group, indicating the patients in the observation group had a higher quality of life than those in the control group. These findings indicate that titanium mesh combine with forearm free flap for maxilary defect repair can be better to maintain the swalowing and language function of patients.
2."Comparison of two oophorocystectomy of surgical procedures based on ""placket suction""and ""helicism suture"""
Chinese Journal of Primary Medicine and Pharmacy 2016;23(20):3068-3071
Objective To explore the clinical comparative value of little abdominal incision and laparoscopic treatment of large ovarian cysts.Methods A total of 31 consecutive cases of large ovarian cysts were analyzed.Two groups were made with the random number table method.Eighteen women with little abdominal incision were served as study group.The corresponding nineteen women with laparoscopic treatment were treated as control group.All the people were treated with placket suctionand helicism suture.The operation time,amount of bleeding,hospitali-zation time,cost of hospitalization and postoperative complications of the two groups were compared,and the levels of C -reactive protein and leukocyte count were also compared.Results The operation time,amount of bleeding,hospi-talization time and cost of hospitalization in the study group were (52.17 ±3.02)min,(30.09 ±1.91)mL,(5.75 ± 0.98)d and (5 272.85 ±355.21)yuan respectively,which in the control group were (72.40 ±4.45)min,(29.71 ± 0.56)mL,(5.15 ±0.31 )d and (11 825.59 ±857.71 )yuan respectively;There were no statistically significant differences in amount of bleeding and hospitalization time between the two groups(t =1.57,1.19,all P >0.05),but the operation time in the study group was less than that in the control group(t =4.51,P <0.05),and the same result of the cost of hospitalization was detected in the two groups(t =5.24,P <0.05).Conclusion In the basis of placket suctionand helicism suture,little abdominal incision surgery method is worthy of the clinical promotion with shorter operation time,more rapidly postoperative recovery and less hospitalization expense.
3.Lung tissue ultrastructure and hypoxia-inducible factor 1 alpha expression in the rats exposed to high altitude hypoxia
Chinese Journal of Tissue Engineering Research 2011;15(37):6905-6908
BACKGROUND: Hypoxia-inducible factor 1α may mediate mammalian cells to adapt hypoxia environment.OBJECTIVE: To observe high altitude hypoxia on the ultrastructure of the lung tissue of hypoxia-inducible factor 1α expression.METHODS: Sprague-Dawley rats underwent high altitude hypoxia intervention for 1, 2, 3 and 30 days. Control group was set. Four high altitude hypoxia groups consisted of Golmud, Qinghai at the altitude of 2 700 m from 5-m above sea level of Xi'an consuming 1 day, Tanggula region at 5 000 m above sea level consuming 2 days, and Tibet Nagqu at 4 500 m above sea level consuming 3 and 30 days, respectively.RESULTS AND CONCLUSION: Light and electron microscopy showed that acute high altitude hypoxia 2 day group presented apparent high altitude pulmonary edema, while the 30 day group of acute high altitude hypoxia of high altitude pulmonary edema was significantly reduced, but hypoxia-inducible factor 1α mRNA expression was significantly increased (P < 0.01). Results demonstrate that hypoxia-inducible factor 1α mRNA expression increase in lung tissues after hypoxic acclimatization can help ameliorate high altitude pulmonary edema.
4.Expression Patterns of Nedd4 Isoforms and SGK1 in the Rat Cochlea
Journal of Audiology and Speech Pathology 2009;17(4):369-372
Objective To investigate the cellular localization of the neural precursor cell-expressed, developmentally downregulated isoforms(Nedd4), Nedd4- 1/2 and Nedd4- 2, and the serum glucocorticoid- inducible kinasel(SGK1) in various subregions of the rat cochlea. Methods The expression patterns of Nedd4-1/2, Nedd42 and SGK1 in the cochlea of rat were studied by immunohistochemistry with the specific polyclonal rabbit antibodies against the rat Nedd4-1/2, Nedd4-2 and SGK1. Results All three proteins were extensively expressed in various regions of the rat cochlea. They were found in the stria vascularis, spiral ligament, organ of Corti, spiral limbus, spiral ganglion and Reissner's membrane. Conclusion Our findings suggest that there exists a Na+ transport system in the cochlea consisting of SGK1, Nedd4 isoforms and ENaC, which may work in concert to transport Na+ and to maintain homeostasis in the inner ear as they do in other tight epithelia.
5.Effect of Gentamicin on the Expression of Subunit Isoforms of Na, K-ATPase in Stria Vascualris of Guinea Pig
Journal of Audiology and Speech Pathology 2000;8(3):137-139
ObjectiveTo investigate the expression of asubunit isforms of Na,K-ATPase in stria vascularis of guinea pig, and the effect of gentamicin on it. MethodsThe expression of a subunit isoforms of Na, K-ATPase in stria vascualris of guinea pig and its change after gentamicin exposure were identified immunocytochemically using the specific mouse antibodies a gainst the α1 ,α2 and α3 subunits of rat Na,K-ATPase. ResultsAll marginal cells,intermediate cells and basal cells of stria vas cualris showed positive expression of α1 isoform and negative expression of α2 and α3 isoforms. The immunostaining of α1 isform was attenuated significantly after gentamicin esposture. ConclusionGentamicin significantly inhibits the expression of Na, K-ATPase α1 subunit isoform of the stria vascularis, sequentially inhibits the activity of Na,K-ATPase.
6.Study on the mechanisms of tumor inhibitory effect of centipede extraction on heterotopic grafting hepatocellular carcinoma in nude mice
Chinese Journal of General Surgery 1993;0(02):-
Objective To investigate the effects and mechanisms of extraction centipede extraction (CE) on grafting hepatocellular carcinoma (HCC) in nude mice.Methods We set up model of Bel-7404 cells heterotopic grafting HCC in nude mice,and observed the tumor growth and metastasis after intragastric administration of CE (treatment group).Immunohistochemical methods were used to detect X-chromosome linked inhibitor of apoptosis protein(XIAP),bax gene,vascular endothelial growth factor(VEGF) and angiopoietin-2(Ang-2) in tumor tissue.Results There was distinctive inhibitive effect of CE on in Bel-7404 cells heterotopic grafting HCC in nude mice.The staining cells population,staining intensity and the optical density(OD)of XIAP,VEGF and Ang-2 in the control group were higher than those in treatment group,but those of BAX were lower than that in treatment group,and there was significant difference between the 2 groups(P
7.Comparative studies on chemical constituents difference between single medicine decoction and mixed medicine decoction of Changweikang Granules
Chinese Traditional Patent Medicine 1992;0(10):-
AIM:To analyze and compare the chemical constituents difference between single medicine decoction(SMD) and mixed medicine decoction(MMD) of Changweikang Granules. METHODS: TLC and HPLC methods were applied to comparing the chemical constituents in SMD and MMD of Changweikang. RESULTS: Spots of TLC between SMD and MMD similar,except for some difference in the HPLC. CONCLUSION: The analyses were carried out for the first time.We found that the plus of the chemical constituents in SMD equal to MMD of Changweikang,also during the course of MMD,new constituents may be generated.Based on these,we proposed that the material base of Changweikang were the rational preparation and complementation of the effective components and the generation of new constituents.
8.A STUDY OF MICROSURGICAL ANATOMY OF THE LYMPHATIC SYSTEM OF LIMBS
Acta Anatomica Sinica 1957;0(04):-
For the need of lymphovenous anastomosis in microsurgery, the following investigations were carried out: the anatomy of the superficial lymphatic system of the limbs (by direct injection method on 40 cadaverous specimens); mimic surgical incisions (on 2 specimens) and the criteria of searching and differentiating the small lymph channels from the small blood vessels and nerves (on 29 cases of limb operations).1. The limb superficial lymph vessels are arranged in two layers, those in the superficial layer are smaller in size but large in number than that in the deeper layer. The large collecting lymph vessels run alongside the chief superficial vein.2. The location, course, number and diameter of the superficial lymph vessels were studied.3. The morphological criteria of differentiating the small lymph vessels from the minute blood vessels and nerves under surgical microscope were suggested.4. The relationships between the distribution of the lymphatic valves and the site of surgical approach were discussed. The valves were found onlyat the distal portion of the "lymph-pearl" and the proximal part of the "pearl" will be the suitable site for surgical incision.
9.Determination of thymidine phosphorylase in patients with lung cancer and its clinical significance
Chinese Journal of Practical Internal Medicine 2006;0(16):-
0.05).The differences between NSCLC and SCLC reached the statistical signifcance,P
10.Reconstruction of spiral CT image transmitting system and its transmitting network
Chinese Medical Equipment Journal 1993;0(05):-
The transmitting network is reconstructed to make the network syetem convenient,stable and swift when transmitting CT image.