1.Safe dosage of intrathecal injection of sufentanil for preemptive analgesia in lower limb orthopaedic surgery
He ZHANG ; Qixiang WANG ; Ruijiang ZHONG
Chinese Journal of Postgraduates of Medicine 2012;35(3):27-30
ObjectiveTo selected the safe dosage of intrathecal injection of sufentanil for preemptive analgesia in lower limb orthopaedic surgery.MethodsSelected 96 patients who ASA classification Ⅰ - Ⅱ grades undergoing elective lower limb orthopaedic surgery with subarachnoid epidural block down,they were divided into six groups by random number table and double blind method,each group of 16 cases.Group Ⅰ,Ⅱ,Ⅲ,Ⅳ,Ⅴ,Ⅵ received 0.05,0.10,0.15,0.20,0.25,0 μ g/kg of intrathecal sufentanil and 2.5 ml of 0.5% levobupivacaine.Observed and recorded the duration of block level of pain to T10,visual analog score (VAS)postoperative at 2,4,6,8,12,24,48 h,and side effects.ResultsThe duration of block level of pain to T10 of group Ⅰ,Ⅱ,Ⅲ,Ⅳ,Ⅴ,Ⅵ was (245.6 ± 34.2),(259.3 ± 36.7),(268.9 ± 37.8),(286.6 ± 38.8),(287.7 ± 39.1 ),(228.6 ± 31.4) min.There was no significant difference between group Ⅳ and group Ⅴ (P > 0.05 ),the differences of other groups were statistically significant (P < 0.05 ).Compared with group Ⅴ,VAS of group Ⅲ and group Ⅳ at 6,8,12,24,48 h after operation was not statistically significant (P > 0.05),and they were lower than the other three groups(P < 0.05 ).There were no respiratory depression occurred after operation in group Ⅰ,Ⅱ,Ⅲ,Ⅳ,Ⅵ,4 cases occurred in group Ⅴ.ConclusionThe best of effective dose of intrathecal injection of sufentanil for preemptive analgesia in lower limb orthopaedic surgery is 0.20 μ g/kg.
2.Overview of head injury caused by the major earthquake of Wenchuan and the diagnostic value of multi-slice spiral CT
Bing MING ; Tangli ZHONG ; Yu HE ; Lan SHEN ; Guoqing HE ; Ghuandong HE ; Shunyuan ZHANG
Chinese Journal of Radiology 2008;42(11):1130-1132
Objective To evaluate the information of head injury caused by the major earthquake of Wenehuan and the diagnostic value of muhi-slice spiral CT(MSCT). Methods A total of 1557 earthquake victims were hospitalized, of which head CT scan was performed in 292 eases, and abnormalities were found in 225 of them. The composition, type of injury and MSCT findings of head injury were retrospectively analyzed. Results CT abnormalities were found in 225 victims, including 66 cases of skull fracture,50 eases of cerebral contusion and laceration, 55 eases of intracranial hematoma, 16 cases of subaraehnoid hemorrhage, 16 eases of subdural collection of fluid,20 eases of foreign substance of scalp,and 2 eases of traumatic cerebral infarction. Operation was performed in 22 of them. Conclusion The incidence of head injury in earthquake was high, and most of them were not severe. The examination of MSCT was rapid, MPR and 3D images can clearly show the lesion of craniocerebral trauma. MSCT can provide more valuable information for clinical therapy in craniecerebral trauma caused by earthquake.
3.Immune parameters to serve as biomarkers and clinical significance in tolerant patients after liver transplantations
Kebo ZHONG ; Peng ZHANG ; Xiaoshun HE ; Qiang LI ; Xiaofeng ZHU
Chinese Journal of Hepatobiliary Surgery 2016;22(2):78-81
Objective To analyze the related immune parameters in liver transplantation recipients to help guide adjusting future immunosuppressants.Methods Fifty-three tolerant recipients after liver transplantation were included in this study.They had normal liver functions and took only one immunosuppressive drug.They were divided into the 3 ~5 years tolerance group (3Y group,n =21) (including 5 years) after transplantation,and the 5 ~ 11 years tolerance group (5Y group,n =32).Another 15 liver transplantation recipients who had twice or more recurrent acute rejections were enrolled as the RJ group and 32 age-matched healthy donors served as the HC group.The natural killer (NK) cells,B cells,memory T cells,regulatory T cells (Tregs) and the subsets were detected by flow cytometry.The plasma TGF-β level was evaluated using ELISA.Results The percentages of NK cells (3Y group 19.00 ± 0.09,5Y group 20.00 ±0.09,HC group 14.00 ±0.07,RJ group 9.00 ±0.03) and total Tregs within the CD4+ cells (3Y group 6.26 ±2.33,5Y group 4.80 ±2.21,HC group 3.04 ± 1.25,RJ group 1.09 ±0.81) in the 3Y,5Y and HC groups were significantly higher than those in the RJ group (P < 0.05).The proportions of resting Tregs (3Y group 0.23 ±0.07,5Y group 0.16 ±0.02,HC group 0.07 ±0.02,RJ group 0.05 ±0.01),active Tregs (3Y group 0.56 ± 0.11,5Y group 0.42 ± 0.15,HC group 0.08 ± 0.02,RJ group 0.05 ± 0.01) and non-suppressive Tregs (3Y group 3.51 ±0.80,5Y group 3.71 ±0.41,HC group 1.44 ±0.14,RJ group 2.15 ± 0.62) increased significantly in the tolerant recipients after liver transplantation when compared with those in the HC and RJ groups (all P < 0.05).No significant differences on B cells,memory T cells and TGF-β level were detected among in four groups.Conclusions The NK cells,total Tregs,resting Tregs,active Tregs and non-suppressive Tregs increased significantly in the tolerant recipients after liver transplantation.These might serve as potential biomarkers for immune tolerance.
4.Clinical effects of selective laser trabeculoplasty in the treatment of ocular hypertension and different course of primary open angle glaucoma
Shaoyang ZHANG ; Li LI ; Shan ZHONG ; Limei HE
Recent Advances in Ophthalmology 2017;37(9):856-859
Objective To compare the efficacy of selective laser trabeculoplasty (SLT) on the intraocular pressure (IOP) reduction in the treatment of ocular hypertension (OHT),early and moderate primary open angle glaucoma (POAG).Methods Non-randomized controlled study was conducted in 120 patients (120 eyes),who were divided into 3 groups according to the patients' condition,including OHT group with 40 eyes of 40 patients,early-stage POAG group (40 eyes of 40 patients) and medium-stage POAG group (40 eyes of 40 patients),aged rang from 16 to 79 years.SLT was performed on the 360° trabecular meshwork using 532 nm frequency-double Q-switched Nd:YAG laser (Selecta duet),with the pulse of 3 ns and spot diameter of 400 nm.The initial energy was 0.6 m J,which decreased at 0.1 mJ interval till champagne bubble appeared.The following-up was postoperative 1 day,1 week,1 month,3 months and 6 months,respectively,and changes in IOP before and after SLT was recorded and analyzed statistically.Resuits Postoperative IOP at different time points was significantly lower than that of preoperation in the three groups,with significant difference (all P < 0.05).There was also significant difference in IOP of the each group at different time points after the operation (all P < 0.05).The preoperative baseline IOP of OHT group,early-stage POAG group and medium-stage POAG group was (25.95 ± 1.80) nmHg (1 kPa =7.5 mmHg),(24.66 ± 1.55) mmHg and (25.62 ± 2.67) mmHg,respectively,approaching significant difference (P <0.05).After eliminating the influence of baseline IOP,intergroup differences of postoperative IOP at each time point in the three groups were statistically significant (all P < 0.05),and intragroup differences were also significant (all P < 0.05).When compared with corrected baseline IOP,the reduction of IOP was the largest in OHT group after SLT,followed by the early-stage POAG group,and the lowest was in the medium-stage POAG group.Conclusion SLT has a good effect on IOP reduction for OHT and POAG,and the treatment effect of SLT declines successively according to the severity of POAG.
5.Logistic regression analysis of the influencing factors of psychological problems faced by college students
Wenjuan ZHONG ; Wanqi HUANG ; Yinyou ZHANG ; Zun HE
Chinese Journal of Tissue Engineering Research 2007;11(17):3454-3456
BACKGROUND: With the rapid development of modern society, psychological problems in college students have been paid high attention by educatienal organizations of psychological health in China. OBJECTIVE: To probe into the influencing factors of psychological problems in college students. DESIGH: Cluster sampling 1∶2 paired observation, case-control study. SETTING: Medical College of Wuhan Polytechnic University. PARTICTPANTS: 1 200 sophomores with the age ranged 19-20 years were selected from Wuhan Polytecnic University by cluster sampling at June 2001, and 145 students with the scores of more than 3 factors greater than 3 points were screened by Symptom Checklist 90 (SCL-90) (including 9 factors of somatization, compulsion, interpersonal sensitivity, depression, anxiety, hostility, phobia, paranoid and schizophrenia. The higher the grade was, the severer the symptom was) to be the psychological problem group. Subjects were selected to be the normal controls at the ratio of 1∶2 with those in the psychological problem group, whose classes, specialties and ages (the difference was less than 2 years) were similar to those of the enrolled patients. 290 students normal in the indexes of SCL-90 were taken as the matched group. Of all subjects in both experiment groups, there were 173 males and 262 females with an average age of 19.2 years. METHODS: ① The subjects in the two experiment groups were investigated of the psychological health by SCL-90 as well as the personality characteristics and Eysenck Personality Questionnaire (EPQ) (For Adult). The EPQ statistic software designed by the Institute for Juvenile and Children, Tongji Medical College of Huazhong University of Science and Technology was adopted to acquire the E score (introversion, extroversion), N score (nervousness), P score (psychoticism), L score (conceal) and quantity form quadrant of E-N coordinate picture, so as to define the tendencies of characters. ② The psychological problem was considered to be the dependent variable, while all relevant factors (The influencing factors of psychological problem included the understanding from parents, relationship with family members, desire for learning, personality characteristics, over-high P score, over-high/low E score, over-high/low N score) were taken as the independent variable. Single-factor Logistic regression analysis was conducted at 1:2. ③ Based on 1:2 single-factor Logistic regression analysis, the variances were selected from factors of P < 0.01 together with the Logistic regression models of multifactor at 1:2 by stepwise regression method.MAIN OUTCOME MEASURES: ① Comparison in the SCL-90 score between the psychological problem group and the normai control group. ② Single-factor and multivariate analysis of the relevant factors of psychological problems. RESULTS: A total of 435 enrolled students were involved in the analysis of results. ① The average score of somatization, compulsion, interpersonal sensitivity, depression, anxiety, hostility, phobia, paranoid and schizophrenia in students of the psychological problem group was higher than that in the normal control group (t=-13.39, 29.64, 27.09, 26.04, 19.52, 18.13, 14.15, 18.61, 22.41, P< 0.01). ② Nine factors were selected in the single factor analysis after the Logistic regression analysis, including 6 protective factors of understanding from parents, relationship with family members, desire for learning, personality characteristics, E > 61.5, N < 38.5, while he other 3 factors (P > 61.5, E < 38.5, N > 61.5) were risk factors. The multivariate analysis showed that factors of relationship with family members, desire for learning as well as personality characteristics were protective factors, while it was a risk factor when N > 61.5.CONCLUSION: The psychological problems in college students relate to the relationship with family members,understanding from the parents, desire for learning as well as the character trait and personality characteristics.
6.Bioluminescence imaging evaluation of the inhibitory effect of lidamycin on lung metastasis of human fibrosarcoma in athymic mice.
Shenghua ZHANG ; Genshen ZHONG ; Hongwei HE ; Xin CHENG ; Yongsu ZHEN
Acta Pharmaceutica Sinica 2011;46(1):45-9
This study is to investigate the inhibitory effect of lidamycin (LDM) and its combination with methotrexate (MTX) on lung metastasis of fibrosarcoma by bioluminescence imaging in athymic mice. A stable luciferase transfected HT-1080 cell line was constructed and the capability to establish experimental lung metastasis in athymic mice was confirmed. The optical imaging system was applied to evaluate the formation of lung metastasis in vivo. In addition, metastatic nodules were counted for the evaluation of inhibition rates. As shown, the fluorescent intensity of luciferase-transfected HT-1080 cells was colinear with the cell population and the minimal detected cell population was 100 cells/well. Optical imaging showed that the fluorescent intensity of treated group was apparently lower than that of the control. The inhibition rates of lung metastasis by LDM alone at 0.025 mg x kg(-1) and 0.05 mg x kg(-1) were 53.9% and 75.9%, respectively, while that of MTX alone at 0.5 mg x kg(-1) was 70.2%. The combination of LDM at 0.025 mg x kg(-1) and MTX at 0.5 mg x kg(-1) showed an inhibition rate of 88.7%. The coefficient of drug interaction (CDI) was 0.82. The results herein demonstrated that LDM alone had strong anti-metastasis effect on human fibrosarcoma HT-1080 and the inhibition efficacy is strengthened when combined with MTX.
7.Application of High Resolution Melting Method in the Detection of GLP1R Gene Polymorphism Site rs3765467
Yuan ZHANG ; Xia HE ; Lei ZHONG ; Junlan CHUAN ; Enwu LONG
China Pharmacy 2017;28(17):2305-2308
OBJECTIVE:To establish the method for the detection of the known glucagon-like peptide 1 receptor (GLP1R) gene mutation site rs3765467(NT_007592.16,position:39065819),and to evaluate its accuracy and practicability. METHODS:Peripheral venous blood samples of 72 healthy subjects were collected in medical examination center of our hospital during Oct. 2015-Feb. 2016. The whole blood DNA was extracted by column extraction method. After amplified by touch down PCR,high reso-lution melting(HRM)method was adopted to analyze amplified product. Sequencing verification by double stranded chain termina-tion method(Sanger sequencing method)was performed for 38 test samples. The results of 2 methods were compared. RESULTS:The results of mutation scanning showed that there were 39065817 and 39065819 polymorphism sites in amplified segments. Four types of mutations were detected by HRM method [GCG/GCG,GCA/GCG or ACG/GCG,GCA/GCA or ACG/ACG,A(G) CA(G)],but 6 types of mutations was detected by Sanger sequencing method [GCG/GCG,ACG/GCG,ACG/ACG,A(G)CA (G),GCA/GCG,GCA/GCA]. CONCLUSIONS:HRM method can identify GCG/GCG and A(G)CA(G)genotype,but can not identify GCA/GCG and ACG/GCG heterozygous mutation,GCA/GCA and ACG/ACG homozygous mutation. The method is not suitable for the detection of single nucleotide polymorphism for multiple neighboring sites. In the detection of single nucleotide mu-tation,economical and simple method should be selected after comprehensive analysis of sequence.
8.Effect of VIM in EV71 infection in human microvascular endothelial cells
Yanyun ZHONG ; Bao ZHANG ; Mingliang HE ; Yujuan CAO ; Xianbo WU
The Journal of Practical Medicine 2016;32(10):1560-1563
Objectives To study the effect of VIM in Enterovirus 71 (EV71) infection of (human brain microvascular endothelial cells (HBMEC) and elaborating the mechanism of EV71 infection in the nervous system. Methods Knocked out the VIM by CRISPR technology , the differences in EV71 absorption , replication , release between wild VIM and VIM knocked-out (VIM-KO) HBMEC were detected by fluorescence quantitative PCR. Results 4 ℃ absorption experiment conformed that EV71 adsorption in VIM- KO is 40% less than in the normal HBMEC. After EV71 infect HBMEC for 48 h (48 h p. i.), the quantitative PCR result showed intracellular viral RNA in VIM-KO was only 1/12 of that in the normal HBMEC. Also the extracellular viral RNA was quantified, and the number of cells in VIM-KO had been reduced 1.4 times compared with the normal HBMEC. Conclusions Once VIM knocking out, EV71 attachment has been obviously reduced. Meanwhile, the level of viral RNA replication and release are decreased compared with the normal HBMEC. VIM may be an attachment receptor of EV71 in HBMEC , when the virus invades HBMEC with the binding of VIM. Moreover , VIM plays an important role in the replication and release of EV71.
9.Investigation and Analysis of Reference Intervals and Decision Limits of Reticulocyte Count in China
Xiaoyan ZHANG ; Wei WANG ; Falin HE ; Kun ZHONG ; Zhiguo WANG
Journal of Modern Laboratory Medicine 2016;31(3):158-161
Objective To investigate and analyze the current situation of reference intervals and decision limits of reticulocyte count in China.Methods The information related to upper/lower limits,decision limits,data resources,method principles, instruments and reagents of reticulocyte count in 2014 was collected via the external quality assessment software system based on website.The participants were divided according to their methods and instruments.The SPSS 1 9.0 was used for conducting the data analysis and statistics of results for each group.Results There were 276 laboratories submitting the val-id results.The major resources for reference intervals came from national guide to clinical laboratory procedures (53.99%), instructions of instrument manufacturers (16.30%),textbook (9.78%),instructions of reagents manufacturers (9.06%) and others.The major resources for decision limits were national guide to clinical laboratory procedures (56.06%),instruc-tions of instrument manufacturers (16.67%),textbook (7.58),instructions of reagents manufacturers (7.58%)and others. The medians and averages of upper/lower limits and decision limits among subgroups were close,whereas the P2.5 and P97.5 were significant discrepancies.Only 45.65% and 48.48% of participant laboratories had verified reference intervals and deci-sion limits before clinic use.The lower limits and decision limits among subgroups had nostatistical difference (P>0.05), where as the upper limits of different instrument groups had significant differences (P<0.001).Conclusion The current situation of reference intervals and decision limits of reticulocyte count is unsatisfactory.It is certain to establish a uniformed reference interval and decision limits for reticulocyte count,which provides a reference for the standardization of reticulocyte count testing.
10.Investigation and analysis of current situation about reference intervals of lymphocyte subsets by flow cytometry tests in China
Shishi ZHANG ; Wei WANG ; Falin HE ; Kun ZHONG ; Zhiguo WANG
Chinese Journal of Laboratory Medicine 2016;39(5):356-360
Objective Discussthe reference intervals and their sources of lymphocyte subsets by flow cytometry tests by all Chinese clinical laboratories at 2014 with a comparison of difference between different test systems.Methods Questionnaires about reference intervals were distributed to 212 clinical laboratories participating in October 2014 national external quality assessment scheme of lymphocyte subsets by flow cytometry tests of National Center for Clinical Laboratory.Relevant information about reference intervals of 5 relative counting items CD3 + (%),CD3 + CD4 + (%),CD3 + CD8 + (%),CD3-CD16 + CD56 + (%) and CD3-CD19 + (%) was collected by a internet-based external quality assessment software systems.Microsoft Excel 2010 and SPSS 19.0 were used to sort out raw data,eliminate unreasonable or incorrect data,and only analyze source distribution,arithmetic average,median,minimum value,maximum value and percentiles (P2.5 and P97.5) of upper and lower limits about healthy adults' reference intervals.Laboratories derived from the highest percentage of source distribution whose reagent and instrument were matching with each other were grouped according to the test systems used and the differences of reference intervals between different systems were compared with Mann-Whitney test.Results For five items collected,in the clinical laboratories who accepted statistical analysis,respectively 39.32% (81/ 206),37.44% (79/211),37.68% (78/207),37.11% (72/194),36.98% (71/192) of them had verified the reference intervals they used.The top three sources of reference intervals were instructions of reagent and instrument manufactures (49.70%-52.54%),calculations by laboratories themselves (18.64%-20.00%),and calculations by other laboratories (14.12%-15.43%).The arithmetic averages and medians of upper and lower limits about every item were relatively close.But the minimum and maximum values of upper and lower limits of certain items had moderate differences.As Mann-Whitney test later showed,all items with the P values of upper and lower limits between different test systems,except for the lower limit of CD3 + CD4 + (%) (P value is 0.052) and the upper limit of CD3-CD19 + (%) (P value is 0.117),were lower than 0.05 which meant an existing statistical difference.Conclusions The sources of lymphocyte subsets by flow cytometry tests in all Chinese clinical laboratories were different and the instructions of reagent and instrument had the highest percentage,but the reference intervals among differenttest systems had statistically significance.