Objective To evaluate the methods and effect of balloon catheter dilation of benign esophageal stenosis in children. Methods 9 cases had an anastomotic stenosis after surgical correction of esophageal atresia; 11 cases of esophageal stenosis due to ingestion of caustics; one case had an lower esophageal stenosis after Nissen surgery and one case after gastroesophagoplasty. Age ranged from 17 days to 7 years. Each case had a barium esophagram before balloon dilation. The balloon size varied from 3 to 10 mm in diameter. Results 21 cases were successful after dilation of balloon catheter. There were no esophageal perforation and complications. The satisfactory results maintained from six months to thirty months. Conclusions Balloon catheter dilation is a simple、safe and reliable method for the treatment of benign esophageal strictures in children as the first choice.

Objective To evaluate the methods and effect of balloon catheter dilation of esophageal caustic ingestion stenosis in children. Methods We analysed 18 cases, including 10 cases of esophageal stenosis due to ingestion of sulphuric acid, 7 cases of esophageal stenosis caused by ingestion of sodium carbonate and the last one through ingestion of chemical materials include zinc sulphate. Barium esophagogram was taken before dilation for every patient and the balloon size varied from 4 mm?40 mm to 16 mm?40 mm or 20 mm?40 mm in diameter was selected for the procedure. Results 18 cases were all successful in dilation by balloon catheter, without esophageal perforation and other complications. The satisfactory results maintained from six months to thirty months with remarkable improvement clinicoly. Conclusions Balloon catheter dilation is a simple, safe and reliable method for the treatment of esophageal caustic ingestion stenosis in children, and should be recommended as the first choice.

Purpose　To explore the clinicopathological features of subcutaneous panniculitic T-cell lymphoma(SPTCL) and significances of genetic analysis in the diagnosis. Methods　Histopathology, immunohistochemitry and detection of clonal gene rearrangement by PCR were used in 3 cases of subcutaneous panniculitic T-cell lymphoma (SPTCL), which were originally diagnosed as relapsing nodular nonsuppurative panniculitis. Results　Three misdiagnostic cases were correctly redefined as subcutaneous panniculitic T-cell lymphoma, with immunophenotype of CD45+,CD45RO+, Mac387－,and clonal TCR-β gene rearrangement. Conclusions　Subcutaneous panniculitic T-cell lymphoma has distinctive clinicopathological features. Genetic analysis is an effective method for the diagnosis of SPTCL.

OBJECTIVE:To study the pharmacokinetics of two kinds of famotidine capsule from different factories in healthy volunteers and to evaluate the bioavailability of them METHODS:A single oral 40 mg dose of famotidine capsule of reference or test preparation was given to healthy male volunteers according to an open randomized crossover study The plasma concentrations of famotidine were determined by a RP-HPLC method The pharmacokinetic parameters and bioavailability of test preparation were compared with reference preparation RESULTS:The main pharmacokinetic parameters of the reference preparation and the test preparation were as follows:Cmax were(189 9?72 1)?g/L and(170 1?59 6)?g/L;Tmax were(2 5?0 8)h and (2 1?0 8)h;T1/2 were(4 1?0 9)h and(3 8?0 8)h;AUC0～t were (1 031 7?316 7)?g/(L?h)and(1 019 4?290 5)?g/(L?h);AUC0→∞ were(1 123 9?346 0)?g/(L?h)and(1 103 4?312 0)?g/(L?h);The relative bioavailability of reference to test preparation was(101 6?22 6)% CONCLUSION:The results showed that the reference preparation and the test preparation were bioequivalent

When we did compatibility testing, we found a case of leukemia with irregular antibody. The antibody specificity was identified as anti-Ce using panel-cell, and was IgG antibody. The anti-Ce antibody had both anti-C and anti-e activity. It is very difficult to find a Ce antigen negative blood for transfusion. The compatible donors rate is very low, only 2%-3%.

Objective To report the results of balloon catheter dilation in 13 infants with anastomotic stenosis after surgical correction of esophageal atresia. Methods Thirteen cases of anastomotic stenosis after surgical correction of esophageal atresia were enrolled, age ranging from 3 to 10 years. Barium esophagography before balloon dilation showed the diameter of esophageal stenosis ranging 1 to 3 mm and the stenosis was dilated by using balloons of 6 to 12 mm diameter. Results All the 13 cases obtained complete resolution of the stenosis after dilation for twice or thrice. There was no esophageal perforation or other complications with a period of clinical symptomless of 6 to 30 months. Conclusions Ballon catheter dilation is a simple, safe and reliable method for treating anastomotic esophageal stenosis in infants.

Objective To evaluate the role of interventional therapy in Wilm's tumor, renal vascular lesions and ureter stricture in children. Methods Thirty-eight cases were analysed, including 18 cases of Wilm's tumor treated with preprocedural chemotherapy and embolization through renal artery, and 13 cases of unknown-reason hypertension with selective renal arterial angiography for venous blood renin including undergone some patients PTA. Six cases of ureter stricture were dilated with balloon and 1 with hematurea by small aerial embolization. Results After embolization, Wilm's tumors showed reduction in vascularization and reduction in size too. The surrounding tissue of the tumor had a clear demarcation with the normal tissue, decreasing the dissemination to blood and distal metastasis. Renal vascular hypertension was clearly diagnosed and treated, and the increase of renin was helpful to the diagnosis of unknown-reason hypertension. And the obstruction of ureter was improved. Conclusions Interventional therapy is feasible in the application of urinary system diseases with great significance clinically.

Objective To evaluate the application and safety of angiography in infants.Methods Eighteen infants with diagnosis of clinics,CT and MRI as hemangioma, hepatic and renal tumor and cerebral vascular diseases were undertaken digital subtraction angiography(DSA). Results All the cases were found to have anomalous lesions including hemangioma racemosum in 3 cases, capillary hemangioma in 1, hepatoblastoma in 3, renal Wilm's tumor in 1, renal rhabdomyosarcoma in 1, liver arteriovenous fistula (AVF) in 1, Galenic arteriovenous malformation (AVM) in 1,Sturge-Weber Syndrome in 1, AVM OR AVF in the craniofacial region in 2 and pre-operation cerebral angiography of PNET in 2. Conclusions DSA in infants is safe and reliable with extensive clinical application.

Objective To evaluate the efficacy and safety of embolization therapy with NBCA for intracranial arteriovenous fistula (AVF) in infants. Methods Four patients with cerebral arteriovenous fistula were reported, including 3 males and 1 female with nonspecific symptoms and signs, and the diagnosis was made by DSA. Three of them were treated by embolization therapy with NBCA. Results Three of the cases were treated by NBCA, neither death nor complication occurred. Conclusion Intracranial AVF is rare in childhood. Endovascular treatment with NBCA is effective and safe, but the long-term effect has to be comfirmed by follow-up study. (J Intervent Radiol, 2006, 15: 451-452)

Aim To develop an in vitro high throughput drug screening system based on reporter gene assay for identification of novel compounds with PXR, FXR and LXRα agonist activity. Methods The expressions of exogenous PXR, FXR and LXRαgene in HEK293, HepG2 and LS174T cells were examined by Real-Time quantity PCR. pSG5-hPXR and pGL3-XREM-CYP3A4, pEGFP-N3-hFXR and EcRE-TK-Luc, pCMX-FLAG-hLXRα and pGL3-XREM-CYP3A4 were cotransfected into cells and the optimal ratio of three plasmids was determined. The dose-response relationship between the positive drug and the fold induction was determined. The specificity of the model was ex-amined, and the repeatability was also determined by Z′ value. Results ① The PXR, FXR and LXRα mRNA expression in HEK293 cell is low among three different cells. ②reporter gene vector and expression plasmid ratio of 1∶ 1, 2∶ 1 and 2∶ 1 were proved to be suitable for highest relative luciferase activity for PXR, FXR or LXRα agonist screening model. ③ The relative luciferase activity was induced by Rif, CDCA or T0901317 in a dose-dependent manner. ④Only Rif, CDCA or T0901317 could significantly increase the relative luciferase activity in PXR,FXR or LXRα agonist screening model, no effect of other nuclear re-ceptors agonist was observed, and the values of Z′-factor for PXR, FXR and LXRαagonist screening model were 0. 58, 0. 66 and 0. 63, respectively. Conclusion An in vitro PXR, FXR and LXRα agonist high-throughput screening models are devel-oped with acceptable specificity and repeatability, and the mod-els can be used to screen PXR, FXR and LXRα agonist.