Objective To explore the psychological state after needle stick injury among nurses.Methods A questionnaire survey was conducted among 256 nurses to understand their psychological state after needle stick injury, and the results underwent analysis.Results 220 nurses suffered needle stick injury among 256 nurses.79% nurses recognized that the serious result after needle stick injury was various blood-borne diseases.91% nuuses felt feared after needle stick injury.70% nurses felt inevitable to suffer needle stick injury. 10% and 11% nurses showed avoidance and turnover idea. Conclusions The majority of nurses show negative and fearful mental status after needle stick injury, but they think it is inevitable and give no sufficient attention to it, besides, the minority of nurses show less recognition to needle stick injury.

Adult ; Bone Neoplasms ; pathology ; surgery ; Female ; Giant Cell Tumor of Bone ; pathology ; surgery ; Humans ; Patella

0.05);there were significant decrease in FPG,PPG and HbA1c in the DM+INS+RSG group after 1 and 3 months treatment(P

Objective To investigate the effect and the mechanism of different G-CSF-priming protocols on leukemia cell lines (HL-60 and U937) in vitro and provide the clinical guidance to clinical treatment of acute leukemia.Methods The leukemia cell lines HL-60 and U937 were used as model to detect the effects of three drugs alone and combined two drugs (HA) or three drugs (HAG) respectively.Cell viability and cell growth inhibition were performed by cell count kit-8 (CCK-8) assay.Apoptotic marker AnnexinV/PI,cell membrane surface antigen CD11b,cell cycle,mitochondrial membrane potential (JC-1) and Caspase-3 were determined by flow cytometry.Results After using of HAG for 48 h,HL-60 and U937 cells counts were decreased significantly and the apoptotic marker Annexin V was significantly increased. To compare the single drug group with two drug combination group,the result was significantly different (P ＜0.05),and the apoptosis of U937 cells was higher than HL-60 cell line.CD11b expression among the three groups did not change (P ＞ 0.05).Using of CAG and MAG,the mitochondrial nembrane potential of HL-60 and U937cells was increased,the three-drug combination group was significantly higher than single-drug group and control group (P ＜0.05); Caspase-3 was activated,the fluorescence intensities of Caspase-3 of the three-drug combination group and single drug group were significantly higher (P ＜0.05) comparing with the control group.Conclusion HAG regimen could induce leukemia cells to apoptosis through the reduction of mitochondrial membrane potential and the activation of Caspase-3 to induce apoptosis of leukemia cells.

AIM: To investigate the effect of epidermal growth factor receptor (EGFR) inhibitor erlotinib on kidney injury in diabetic nephropathy (DN) rat and the underlying mechanism.METHODS: The rat model of DN was induced by intraperitoneal injection of streptozotocin (STZ) at dose of 55 mg/kg.One week after STZ injection, the rats with blood glucose level exceeding 16.7 mmol/L were identified as diabetic.Diabetic rats were randomly divided into 2 groups: STZ group and STZ+erlotinib group.In addition, the normal rats were used as control group.The rats in STZ+erlotinib group were treated with erlotinib at 100 mg·kg-1·d-1 for 4 weeks(5th~8th week).The fasting blood glucose (FBG), serum creatinine (SCr) and 24 h urine protein were measured.The pathological changes of the kidney were observed by HE staining and Masson staining.The protein levels of EGFR, p-EGFR, transforming growth factor β1 (TGFβ1), Smad2/3, p-Smad2/3, collagen Ⅳ (ColⅣ) and fibronectin in the kidney tissues were determined by Western blot.The reactive oxygen species (ROS) level and malondialdehyde (MDA) content in the renal tissues were futher analyzed.RESULTS: Compared with control group, the levels of FBG, 24 h urine protein and Scr were significantly increased in STZ group (P<0.01).Compared with STZ group, the levels of FBG, 24 h urine protein and SCr in STZ+erlotinib group were markedly decreased (P<0.05).In additon, the glomerular structure was restored to normal, the proliferative degree of mesangial cells markedly attenuated, and the epithelial cells were in alignment in STZ+erlotinib group.Moreover, erlotinib significantly inhibited the protein levels of p-EGFR, TGFβ1, p-Smad2/3, ColⅣ and fibronectin in the kidney tissues of STZ rats.In addition, erlotinib also significantly inhibited the levels of ROS and MDA in the kidney tissues of STZ rats.CONCLUSION: Erlotinib ameliorates STZ-induced diabetic nephropathy possibly through inhibiting the activation of EGFR/TGFβ1-Smad2/3 signaling pathway in association with suppression of fibrosis and oxidative stress.

The development of science and technology not only promotes the development of the society,but also has a great influence on the progress of medicine.The three dimensional (3D) printing technology together with other digital production modes will promote the realization of the third industrial revolution.New 3D equipments and concepts brought a huge leap of surgery,and pancreatic surgery is also benefited from this.The author has committed himself to the scientific research of 3D pancreatic surgery for decades,and applying 3D visualization technology of the diagnosis and treatment of pancreatic cancer,3D visualization of peri-pancreatic vessels,resectability evaluation of the pancreatic cancer and prevention of pancreatic fistula.This article presents a new idea on the development of pancreatic surgery and introduces not only the progress in the diagnosis and treatment of pancreatic diseases in the era of digital medicine,but also the relative advantages brough about by the new equipments (such as da Vinci robotic surgical system,3D laparoscope).

Objective To study the effects of di-(2-ethylhexyl) phthalate(DEHP) on the organs of rats.Methods Male Wister rats were divided into five groups,four exposure groups and one control group,10 in each.The exposure groups were given with DEHP at the doses of 1 500 mg /kg,3 000 mg /kg,4 500 mg /kg,6 000 mg /kg respectively,by gavage,28 consecutive days.The rats were weighed every 4 days,after 28 days,the rats were killed and the organ coefficients were calculated and the pathological examination was done.Results The weight of four exposure groups reduced significantly(P

OBJECTIVE: To offer guidance for psychiatric patients about rational drug use and improve patient's compliance.METHODS: Clinical pharmacists answered the questions encountered by patients during medication process by means of face-to-face talk with patients or their relatives and telephone inquiry.RESULTS: Patients' drug counseling in psychiatry department covered wide range,much as in the aspects of drug interactions and adverse drug reactions.Due to medication consultation service,patient's consciousness of rational drug use had been improved and some medication errors had been avoided,clinical pharmacists' professional knowledge has been enriched and clinical pharmacy has been enhanced and developed.CONCLUSION: The medication consultation service developed in psychiatric department is of positive practical significance in promoting rational drug use and improving clinical efficacy.

Objective: To develop the Padua Inventory(PI) for the use of Chinese college students;explore the structure and examine the sex difference in non-clinical sample in China.Methods: The PI was filled by 1300 college subjects,including 680 males and 542 females(78 gender missing),aged 19.6?2.2,for exploratory factor analysis(EFA).The second sample has 30 college students from Peking University for test-retest study.Results: The results of EFA has four factors: impaired control over mental activities/doubting,worries of losing control over motor activities,contamination,and checking.Reliabilities of four factors are 0.94,0.85,0.86,0.83 for each and 0.96 for the total scores.The test-retest correlations of the four factors are 0.77,0.87,0.85,0.84 for each.Females scored significantly higher in factor 3(P=.000) while males scoring significantly higher in factor 4(P=.017).Conclusion: The results suggest that PI has eligible psychometric reliability,but needs further study for the validity in China.

Objective To study the expression changes of matrix metalloproteinases (MMPs), protein kinase C (PKC) and nuclear factor-?B (NF-?B ) on the injured rabbit vascular smooth muscular cells (VSMC) transfected with tissue inhibitor of metalloproteinase-2 (TIMP-2) vector. Methods Lipofectin method was used to transfect TIMP-2 vector into VSMC, Western blot analysis to detect TIMP-2 peptides and zymography assay to determine MMPs. The activities of MMPs and NF-?B were detected by electrophoretic mobility shift assay (EMSA). The mRNA and protein expressions of PKC? were determined by RT-PCR and immunocytochemistry. Results The injured VSMC showed increased enzyme activity of MMP-2. There was very lower level expressions of PKC? and NF-?B in the normal VSMC but high in the injured VSMC. However, in injured VSMC transfected with TIMP-2 vector, the activity of MMP2/9 was suppressed and the expressions of PKC? and NF-?B decreased (P