AIM:To explore the production and cytotoxicity of the reactive oxygen species(ROS)induced by diallyl trisulfide(DATS)in HL-60 cells.METHODS:HL-60 cells were either treated with various doses of DATS alone,or DATS combination with apocynin,a specific NADPH oxidase inhibitor,or with antioxidant N-acetyl-L-cysteine(NAC)for 0,1,3,6,12 and 24 h,respectively.The intracellular ROS level was measured by flow cytometry.The activity of NADPH oxidase was evaluated by NBT reduction experiment.The content of both malondialdehyde(MDA)and the protein carbonyl were analyzed by spectrophotometer.RESULTS:The results from flow cytometry indicated that DATS significantly increased the intracellular ROS level in HL-60 cells(P

Objective:To purify a kind of deoxyribonuclease from earthworm Eisenia foetida (named earthworm DNase, EDNase) and study its characteristics. Methods: Acetone precipitation, ion-exchange chromatography, high performance liquid chromatography, SDS-PAGE, CapiUary electrophoresis isoelectric focusing and MALDI-TOP MS were used for the study. Results: This purified protocol improved 137-fold purification and 45.6 % recovery of enzyme activity. The molecular mass of EDNase was estimated to be 63 000. Mg2+ , Mn2+ and Ca2+ were strong inhibitors of EDNase, while Na+ slightly increasd the enzyme activity. The enzyme was completely stable in the pH range from 4. 4 to 5.2 and had a pH optimum of 4.8. The optimum temperature was 37℃ and the enzyme was stable up to 40 ℃. The pI of the enzyme was 6. 20. Km and Vmax for the enzyme were 1.52 g/L and 4. 89 mg/(mL ·min), respectively, with calf thymus DNA as substrate. The enzyme was able to degrade chromosemal DNA, linear λbacteriophage DNA as well as supereoiled plasmid DNA, but didn' t display any RNase activity. Conclusion: This kind of deoxyribonuclease possesses unique characteristics, which is different from the deoxyribonucleases which we have known before.

Objective To explore the relationship between the characteristics of X-ray and magnetic resonance imaging(MRI) and the expression level of estrogen receptor(ER),progesterone receptor(PR)and human epithelial factor receptor 2(HER2)in breast cancer patients.Methods 108 cases of breast cancer patients in the first 74 Hospital of the PLA from January 2013 to Janu-ary 2017 were selected as the research object.Clinical,pathological and imaging data of patients were collected.All patients were ex-amined by X-ray and MRI after admission.The expressions of ER,PR and HER2 in cancer cells were detected by immunohisto-chemical staining.Results The expression of ER,PR and HER2 was positive in 73 cases(67.59%),63 cases(58.33%)and 43 ca-ses(39.81%),respectively.The positive rate of HER2 in patients with calcification was higher than that in patients without calcifi-cation(P<0.05).The positive rate of PR in patients with calcification along the duct was lower than that in patients of whom the calcification was not along the duct,and the positive rate of PR in patients with glandular or mixed density was higher than that in patients with structural disorder(P< 0.05).The positive rate of ER was higher in patients with calcification,mass,spiculation signs,glandular or mixed density(P<0.05).There were significant differences in the positive rate of HER2 between patients with different shapes and different enhancement modes(P<0.05),and there was a significant difference in the positive rate of PR be-tween patients with different enhancement modes(P<0.05).Calcification displayed by X-ray mammography was positively corre-lated with HER2,calcification along the duct was negatively correlated with the expression of PR,calcification with mass was posi-tively correlated with ER and negatively correlated with PR,spiculation sign was positively correlated with ER,and structural disor-der was negatively correlated with ER and PR.Mass type and inhomogeneous enhancement of MRI signs were negatively correlated with HER2,irregular shape was positively correlated with HER2,and inhomogeneous enhancement was positively correlated with PR (r = 0 .363 ,- 0 .375 ,0 .368 ,- 0 .326 ,0 .426 ,- 0 .489 ,- 0 .373 ,- 0 .468 ,- 0 .403 ,0 .391 ,0 .425 ;P < 0 .05) .Conclusion There is a correlation of signs of X‐ray mammography and MRI signs with the expression of ER ,PR and HER2 .Imaging changes can predict the expression of some biological factors of breast cancer ,which is related to hormones ,chemotherapy sensitivity and the prognosis of patients.