Objective To verify the linear range of WBC measured by CD3700 hematocyte counter. Methods The high-valued specimen was prepared with the enriched white coat,and diluted as a series of concentrations of specimens with the physiological saline. Every specimen was measured for twice,and the sequence of specimens was random. And the Grubbsper test, polynomial regression anal-ysis,and random error estimation were performed. Results There is no outlier in the test results. Re-gression analysis showed it displayed linear in the range of 0 to 219 × 109/L,and the random error was within the allowable limit of our laboratory. Conclusion The linear range of WBC measured by CD3700 hematoeyte counter is coincident to what the manufacturer claims.

Objective To certify the linear range of WBC measured by CD3700.Methods Use the white stratum of blood after centrifugation.Then,the high value specimens were diluted with saline to get a series of samples with different concentrations.Every specimen was measured twice in order to keep the sequence of specimens randomly.The perform outlier test,polynomial regression analysis,and the random error estimation were carried out.Results There was no outlier in the results.It was demonstrated by polynomial regression to be linear from(0～219)?109 L-1.The random error was within the allowable limit of 1/4CLIA'88(3.75%).Conclusion The linear range of WBC measured by CD3700 is just like that of the manufacturer claims.

Patient,competition and change are characteristics of the modern hospital manage environment,clinical laboratory management must be more scientific and more religious.This article lists the main items of clinical laboratory management using ISO15189,and raised some idea on clinical laboratory management using ISO15189 and points out the difficulties of popularization using ISO15189 in clinical laboratory management.

【Objective】 To understand the frequency and significance of anti-" Mi^a" (anti-" Mi^a" mixtures of antibodies) in local population in Zhongshan, and the influence of different experimental conditions on the activity of human anti-" Mi^a" . 【Methods】 The microplate-based agglutination assay and polybrene method were used to screen anti-" Mi^a" in 3 587 blood samples from voluntary blood donors and patients using O type red blood cells with positive Mi^a antigen, then.rechecked by tube method and microcolumn gel card method. 【Results】 The frequency of anti-" Mi^a" was 1.06% (38/3 587), among which 60.5% (23/38) were IgM and 39.5% (15/38) were mixture of IgM and IgG; 0.61% (13/2 135) in local blood donors and 1.72% (25/1 452) in patients(P<0.01). 65.8% (25/38) of the population carrying anti-" Mi^a" had a history of immunity. 57.9% (22/38) were identified to be anti-" Mur" and 42.1% (16/38) anti-" Mi^a" using GP.Vw erythrocyte. The appropriate incubation time for anti-" Mi^a" test was 10 min. 【Conclusion】 The frequency of anti-" Mi^a" was relatively high among blood donors and patients in Zhongshan, and most of the anti-" Mi^a" carriers had a history of immunity. Most anti-Mi^a antibodies were active in saline, and some of them were mixture of IgM and IgG. It may be helpful to include Mi^a positive red blood cells in the irregular antibody screening cell panel to improve the safety of blood transfusion.

【Objective】 To investigate the frequency of platelet antibodies in voluntary blood donors and patients in Zhongshan, Guangdong Province, and to study the specificity and cross-matching of platelet antibodies. 【Methods】 Platelet antibodies of blood donors and patients were screened by solid-phase immunoadsorption (SPIA), rechecked by flow cytometry (FCM), and antibody specificity was identified by PakPlus enzyme immunoassay, and platelet cross-matching was simulated by SPIA. 【Results】 A total of 1 049 blood donor samples and 598 patient samples were tested, with 6 (0.57%) and 49 (8.19%) samples positive for SPIA,respectively(P<0.05); In SPIA positive samples, the positive concordance rate of FCM in blood donors and patients was 100% vs 95%, and that of enzyme immunoassay was 100% vs 88%. Among the initial screening positive samples of blood donors, 5 were anti-HLA Ⅰ antibodies, accounting for 83%, and 1 was anti CD36 antibody, accounting for 17%, with an incidence rate of 0.10%. Among the 14 samples of enzyme immunoassay positive patients, 2 were anti-GP Ⅱb/Ⅲa, 1 was anti-GP Ⅱa/Ⅱa, 8 were anti HLA Ⅰ, and 3 were mixed antibodies (HLA Ⅰ, GP Ⅱb/Ⅲa, GP Ⅰa/Ⅱa). According to the types of antibodies, HLA Ⅰ antibodies were the most common, accounting for 65% (11/17), followed by HPA related anti GP, accounting for 35% (6/17). The majority of patients had a platelet antibody positive typing rate below 30%, accounting for 71.4% (10/14). 【Conclusions】 The positive rate of platelet antibody of patients in Zhongshan area is significantly higher than that of voluntary blood donors, and most of them are anti-HLA Ⅰ and anti-GP, and the incidence of anti-CD36 is extremely low. Therefore, it is necessary to establish a known platelet antigen donor bank, and at the same time, carry out platelet antibody testing and matching of patients, which is helpful to solve the issue of platelet transfusion refractoriness.