0.05);but the difference on every other items in CSQ was significant(P

Objective To build high availability cluster using VMware to improve availability of hospital information system.Methods Virtual machines were installed in VMware server to act as backup servers.High availability software continuously detected status of physical servers using heartbeat signals.Results When physical servers failed,high availability cluster switched services automatically.The business switched to virtual machine quickly and smoothly.Conclusion High availability cluster based on VMware can improve HIS availability and lower implementation costs effectively.

OBJECTIVE:To investigate the chemical constituents of the ear of Schizonepeta mulifida (L.)Briq. METHODS：Ingredients of the ear of this plant were separated by means of solvent extraction and chromatography on silica gel and polyamide and activated charcoal. RESULTS：Three components were isolated. Their structures were elucidated based on the spectral analysis. One of them is a known compound i.e. dehydrosylvestrene (V). The other two were new compound,named neoenneaanetetraoic acid (Ⅶ)，and 3-imino-N-nitrogen substituted acetamide cyclic buty amide (Ⅷ). CONCLUSION：These 3 compounds were isolated from the plant for the first time.By the way, a mixture of n-alkane was also obtained which contains seventeen n-alkane compounds proved by GC-MS.

Objective To study the relationship between the pregnant women with HBsAg and the gestational diabetes mellkus.Methods Clinical data of 94 cases with pregnant women with HBsAg-positive in our hospital were analyzed retrospectively.119 cases with pregnant women with HbsAg-negative were selected at random according to their personal preference and were compared.The prevalence rate of the gestational diabetes mellitus and itS related factors were compared between the 2 groups.Results 32 cases were diagnosed the gestational diabetes mellitus in pregnant women with HBsAg-positive,according to 34.0%,13 cases were diagnosed the gestational diabetes mellitus in control group,according to 10.9%.It was significant difference in 2 groups(P＜0.05).Compared wlth the control group,there were significant difference in the occurrence of thrombocytopenia,post partum hemorrhage(P＜0.05).Compared with the control group,there were no significant changes in the ratio of cesarean section,BMI,occurrence of fetal macrosomia.Conclusion The prevalence rate of the gestational diabetes mellitus and post partum hemorrhage and thrombocytopenia in pregnant women with HBsAg-positive group was significantly higher than in the HBsAg-positive group.

Objective To explore the effect of misoprustol on restoration of gastroinete stinal function after cesarean section.Methods 100 patients after cesarean section were randomly divided into two groups:50 patients were treated by dropping rectum with misoprostol 200μg.The other 50 patients were the control group.To compare the two groups of the time of wind breaking,the time of lactation and postpartum hemorrhage.Results The duration of there recovery time of the gastrointestinal functionin and lactation of misoprostol group was shorrer than that in the control group(P＜0.05).Quicker involution of uterus and less quantity of red lochia were compared with the control group(P＜0.05).Conclusion Misoprostol used after cesarean section can accelerate lactation and wind breaking and the involution of uterus.It would he used clinically for its safety,eonvenieney and without evident side effects.

Objective To evaluate the therapeutic efficacy of peritoneal dialysis(PD) for acute renal failure(ARF) subsequent to cardiac surgical procedures in adults.Method Clinical data of 125 consecutive adult patients,admitted from Feb.1997 to Nov.2007,with ARF subsequent to cardiac surgery and undergone PD,were analyzed retrospectively.The levels of creatinine(Cr),blood urea nitrogen(BUN),albumin and kalium ion([K+]) were compared before and after PD.Results Of 8451 consecutive adult patients undergone operations on heart,358 cases(4.2%) experienced ARF,and 125 cases(1.5%) required PD.In 117 patients performed PD,[K+] declined to normal level within one day,BUN and Cr were 10.5?3.6mmol/L and 129.3?41.6?mol/L at the third day after PD respectively,significantly lower than those before PD(P

The stretched preparation of subcutanous tissue of 120 male rats were observed by means of histochemical methods for separate and consecutive demonstration of norepinephrine and cholinesterase (ChE). In addition to adrenergic nerves, there are acetylcholinesterase (AChE) containing nerves surrounding the small arteries and arterioles. AChE is recongnized as a marker of cholinergic nerves in rat subcutanous tissue. After sympathetic gangliectomy, all adrenergic and most of cholinergic nerves disappeared, we suggested that both of them are terminals of sympathetic postganglionic nerve fibers. Using the method for the consecutive demonstration of norepinephrine (NE) and AChE, it showed a dual innervation at the same site of an arteriole, most of them are superimposed each other, their distribution were just the same; but the other one third were not. The latter is a separate sympathetic cholinergic system. Whether these superimposed terminals were coming from different neurons and travelling in the Schwann cell or they contained two kinds of neurotransmitters in the same neuron terminal were discussed.By the small arteries and arterioles of rat subcutanous tissue, there were many ChE-positive nerves, which were sensitive to iso-OMPA inhibition. Such thin unmyelineted nerve fibers are mainly non-cholinergic. They showed a variety of free nerve endings in the martrix of connective tissue, and they can be traced, in association with the whole course, to the small spinal nerve trunk that travelling in subcutanous tissue. We consider that these ChE-positive nerves and their terminals are sensory components of cerebrospinal fibers. It has been observed that some of free nerve endings are superimposed with adrenergic paravascular plexus by means of consecutive method.

OBJECTIVE:To realize the current situation of patient's compliance with medication in Tibetan and to evaluate the methods of increasing patient's compliance METHODS:Questionnaire inquisition was adopted in 188 Tibetan patients to investigate the type of diseases,situation of medication and condition of compliance RESULTS:The compliance with medication was related with the type of diseases,patient's educational background and knowledge about diseases It was a good way to raise the patient's compliance that the patient's recognition of diseases and pharmacotherapy should be improved and communication between doctor and patient and guidance on drug use should be enhanced CONCLUSION:Aiming at patients in different ages,the subject matter of medication consultation service should not be the same

OBJECTIVEThis study aims to investigate the expression of connexin 43 (cx43) gene during early development in zebra fish and provide a foundation for further research of cx43 gene in tooth development.

METHODSTotal RNA was extracted within 72 h after fertilization of zebra fish embryos and then reversed transcribed to generate the cDNA library. The specific fragments of the cx43 gene were then cloned and connected to the PGEMT vector. After confirming the constructed plasmid, the corresponding RNA polymerase was chosen, and the digoxin-labeled anti-sense mRNA probe of cx43 was synthesized in vitro. The cx43 gene expression of zebra fish indifferent stages was carried out by in situ hybridization. The relationship of the cx43 gene expression and anatomy of the pharyngeal teeth were compared by alizarin red staining.

RESULTSThe mRNA antisense probe of cx43 was acquired. The positive signal of sepia was observed in the different stages of zebra fish pharyngeal teeth after fertilization. After fertilization for 9 days, the expression site of cx43 in situ hybridization was overlapped in accordance with the anatomical site of the pharyngeal teeth.

CONCLUSIONcx43 gene participates in tooth development and mineralization process and plays a crucial role in later mineralization.

Animals ; Connexin 43 ; Gene Expression ; Genetic Vectors ; In Situ Hybridization ; Odontogenesis ; Plasmids ; RNA, Messenger ; Tooth ; Zebrafish

Objective:To observe the expression of cytochrome P4502E1 (CYP2E1) in the liver tissue of rats with non-alcoholic steatosis hepatitis (NASH) induced by high-fat diet, and effect of total flavonoids of Chinese hawthorn leaf(TFHL). Methods: The model rats of NASH were induced by 12 weeks' high-fat diet, and treated with TFHL and Essentiale. The pathological changes of rats liver were observed, the amounts of liver MDA, and the activities of SOD, GSH-Px were detected, the mRNA and protein expressions of CYP2E1 were detected with RT-PCR and immunochemical method. Results: ①there were serious steatosis, different degree of infiltration of inflammatory cells and necrosis focus in the livers of model rats, the amounts of liver MDA was higher than that of the normal group, the acttivities of SOD, GSH-Px in the livers were lower, the expressions of mRNA and protein were strengthened. ②the expression of CYP2E1 of livers has a positive correlation with the amount of MDA, but negative correlation with the activity of SOD and GSH-Px. ③the inflammation of liver, the amounts of liver MDA level, the mRNA and protein expressions of CYP2E1,the activity of SOD and GSH-Px in rats treated with high or low dose TFHL were obviously lower than the model rats, but the activities of SOD and GSH-Px were higher than the model rats.Conclussion: CYP2E1 takes part in the onset and development of NASH induced by high-fat diat, TFHL can restrain the activity of CYP2E1 and the lipid peroxidization, enhance the ability of antioxygenation of liver cells, and prevent the further development of NASH.