Histone codes are characterized by the type, location, and modification of amino acids in proteins. Recent research has shown that histone codes are closely related to glioblastoma (GBM). In GBM cells, the combined effects of various effector proteins and histone-modified target sites control the state of chromatins, which further affects the epigenetic phenomena, including GBM cell DNA replication and gene expression and regulation. GBM histone code information exists in histone acetylation, methylation, phos-phorylation, ubiquitination, and other post-translational modification processes. Briefly, this study on the relationship between histone code and GBM provides further molecular targets to develop the clinical diagnosis and treatment of GBM.

Stem cell treatment for traumatic brain injury is the focus of current research.But there are still a lot of problems for stem cell transplantation,for example Immune rejection,low survival rate,and so on.Research on the recruitment of autologous bone marrow mesenchymal stem cells to the injured part,and directionally induce the differentiation of neurons to repair the damage,thus become a research hotspot for the craniocerebral injury treatment.Vascular endothelial growth factor (VEGF) and its receptor are widely distributed in the central nervous system.VEGF can promote cerebral microcirculation remodeling and induce MSCs to different toward the blood vessel endothelium.It can also inhibit neuronal death and apoptosis and directionally recruit and induce autologous neural progenitor cells and MSCs to different toward nerve cells.Formed between VEGF,MSCS and neurotrophin network mechanisms to promote brain repair.This self repair treatment is expected to be a good prospect of research plans.

Objective To fully realize laboratory information sharing, giving full play of automation and intellectualization of laboratory devices and improving the management level and work efficiency. Methods A new network architecture method was adopted. The laboratory devices were connected by joining TS into the master network. A computer installing interface management program was used as device server. All laboratory instruments were managed by the interface management program. Results The new LIS realized the total inosculation of LIS and HIS and the connection method of different communication modes on many serial communication devices. Conclusion The method strengthens the system's network integration. The maintenance costs and PC resources are saved.

Objective To explore the whole framework concept of Matrix Clinical Quality in Team Education Model.Methods This paper analyzed the evolution process and basic situation of the volunteer team,summarized the characteristics of the framework concept of the team and the significance of this concept on the team education and member’s comprehensive quality.Results A whole framework concept of Matrix Clinical Quality in Team Education Model was put forward,the fundamental organization framework was set up and the definition of matrix.was clarified Conclusion This concept of MCQ in Team Education Model presented a new education model for medical students.

Objective The aim of this study was to assess the value of △Np63α in predicting tumor recurrence after curative resection in esophageal squamous cell carcinoma (ESCC) patients.Methods We analyzed △Np63α protein cxpression in 304 clinicopathologically characterized ESCC cases by immunohistochemistry.Results We found △Np63α expression was positive in 122 (40％) of 304 cases.△Np63α expression was higher in the cancer tissue than in non-tumorous control tissue at protein level(P =0.034).There was a significant difference of △Np63α expression in patients categorized according to invasive depth (P =0.001),tumor position (P =0.001) and lymph nodes metastasis condition (P =0.001).Multivariate analyses showed that △Np63α was an independent prognostic marker for ESCC recurrence.Conclusion △Np63α is associated with outcome of ESCC and can be a novel predictor for poor prognosis of ESCC patients after curative resection.

A total of 105 patients with ketosis-onset diabetes were divided into four groups (A+B-,A + B +,A - B -,A - B +) based on the results of islet-associated autoantibodies (A +,A - ) and β-cell function (B +,B - ).Nearly 94％ patients in the A -B + group and 1/10 patients in the A - B - group were non-insulin dependent.While in the A + B - and A + B + groups,the detection rates of subsequent non-insulin dependency were 20％ and 58％,respectively.Among the four groups,patients in the A - B + groups had a lower level of IL-6 and a higher level of TNF-α. Patients in the A + B - and A + B + groups had a higher level of IL-18.Our study indicates that patients with ketosis-onset diabetes may present different clinical characteristics. Evaluate the islet-associated autoantibodies and pancreatic β-cell function may be helpful to the clinical diagnostic and therapeutic strategies in diabetes.

Objective To investigate the feasibility of using superparamagnetic iron oxide(SPIO)as MRI contrast agent to assess rat nonalcoholic steatohepatitis Kupffer cells(KC)function.Methods Twenty male SD rats were randomly divided into A and B groups,group A(n=10)was the experimental group fed high fat diet,group B(n=10)was the control group fed normal diet After 8 weeks,plain MR and SPIO enhanced MR were performed in all the rats.Blood lipids were measured,and HE and Perl's blue staining in all livers specimen was done.The related results of the staining were analyzed with t test Results Group A TC and TG levels[(6.58±1.25)and(1.53±0.23)mmol/L respectively]were significantly higher than group B[(1.64±0.22)and(0.55±0.14)mmol/L respectively](t=11.716 and 11.588,P＜0.01).Group A and B groups hepatic signal intensity decreased in all sequences after SPIO enhanced,but in group A the level of decline[(34.78±4.51)% and(60.38±3.49)% respectively]was less than group B[(64.96±2.42)% and(81.08±1.66)% respectively]on PDWI and T_1WI,and statistically significant differences(t=-18.451 and-16.240,P＜0.01)were found.In group A the ratio of signal intensity of liver to spleen(1.002±0.141,5.000±0.516,20.004±1.490 and 2.601±0.077 respectively)was more than group B(0.400±0.102,1.500±0.115,0.503±0.105 and-0.300±0.058)before and after contrast enhancement on PDWI,T_2WI,T_2~* WI andT_1WI(t=10.745,19.800,39.168 and 92.785,P＜0.01).Typical histological hepatic lesions of NASH were observed in group A,Perl's staining-positive particles in group A(2.33±0.50)were fewer than in group B(4)(t=-10.000,P＜0.01).Conclusion The high-fat diet induced model of SD rats was close to the human NASH and was easy to establish.Clinical application of SPIO enhanced MR successfully assessed the phagocytic activity of KC in the study,and it suggested that the pathogenesis of NASH was related to the decreased phagocytic activity of KC.

Objective To investigate whether the defective interfering(DI) particles of Paramyxovirus, Tianjin strain could be used as a candidate adjuvant. Methods DI particles were separated and purified. After being identified, it was equally mixed with the inactivated poliovirus (PV) vaccine. Kunming mice were administered subcutaneously with the mixture, besides we set groups of inactivated PV vaccine containing Al(OH) 3 as an adjuvant, inactivated PV vaccine of which the dose was doubled without any adjuvant, and negative control. Sera were collected in the 14th day after immunization, and the specific antibodies of PV were detected. T/B lymphocyte stimulation indexes(SI) were counted through the lymphocyte proliferation tests. The quantities of IFN-γ/IL-4 produced by the splenocytes which were stimulated again by PV antigen were detected. Results The SI and the quantity of IFN-γof the mice in the group of inactivated PV vaccine combining with DI particles of Paramyxovirus, Tianjin strain were more than other groups. Conclusion DI particles of Paramyxovirus, Tianjin strain could enhance the immune response of inactivated poliovirus, especially the cellullar immunologic response of Th1 type.

Objective To study the phonetic characteristics of patients with abnormal plosive consonant of functional articulation disorders (FAD) and the possible treatment approaches.Methods A total of patients of 4~26 years old with abnormal plosive consonant of FAD received speech assessment and the phonetic characteristics.Incorrect articulation patterns and forms of plosive consonant /b/,/p/,/d/,/t/,/g/ and /k/ were analysed.The targeted speech treatment was established to correct the erroneous places and types of pronunciation.Results The error rate of /t/ (82/87)was the highest,followed by /k/(77/87),/d/(67/87),/g/(60/87),/p/(59/87) and /b/(3/87) which was the lowest.The error rate of fricative was 65.52% as the highest among all the erroneous patterns,followed by unaspiration(63.22%),forward movement of the tongue(54.02%),distortion(33.33%),bilabial(32.18%),and backward movement of the tongue(18.39%).Seventy-one cases were completely cured,and 16 cases partially cured after 2 to 10 times of treatment.The number of incorrect words of plosives decreased to 4.03±2.71 after the speech therapy from 67.97±18.56 prior to that.The differences were statistically significant (t =34.301,P<0.001).Conclusion The articulation errors of plosives occur mainly on /t/,/k/,/d/,/g/and /p/,/b/.The incorrect types of pronunciation are fricatives,unaspiration,distortion and omission.The incorrect places of articulation are forward movement of the tongue,bilabial and backward movement of the tongue.The targeted speech therapy established according to the erroneous places and types of articulation is significantly effective.

Objective To evaluate functional activity of the subcortical nuclei in Wilson's disease (WD) using resting state functional MRI (rs-fMRI),and to evaluate damage to the functional conjunction in the extracorticospinal tract in WD patients.Methods Twenty-two patients with WD (between January 2015 and January 2016),including 18 with cerebral type and 4 with hepatic type,and 20 age-matched healthy controls were enrolled.Neurological symptoms were scored using the modified Young Scale.Patients with cerebral type WD were divided into 4 subgroups.All study subjects underwent rs-fMRI of the brain.The values of amplitude of low-frequency fluctuation (ALFF) and regional homogeneity (REHO) in the thalamus,caudate nucleus,putamen and globus pallidus were determined.The relationships between rsfMRI metrics and clinical status were evaluated.Results ALFF values were lower in the caudate nucleus,putamen and right thalamus of WD patients than in controls (t =-3.07,-3.00,-3.12,-2.46,-2.20;P =0.005,0.006,0.004,0.020,0.036),while REHO values were lower in the left caudate nucleus and left thalamus of WD patients (t =-2.38,-2.16;P =0.025,0.040).In the caudate nucleus (P =0.032,0.029,0.023),thalamus (P =0.022,0.041,0.035) ALFF values were lower in group 4 than in other groups.REHO values of the putamen (P =0.040,0.017,0.040) and thalamus (P =0.024,0.029 7,0.041) were higher in group 4 than in other groups.ALFF values in the caudate nucleus (t =-0.29,P=0.037),and thalamus (t =-1.77,P =0.042) were lower,and REHO values in the caudate nncleus (t =-1.46,P =0.040) were lower,in patients of cerebral type than in hepatic type patients.Conclusions The damage to the functional activity of the subcortical nuclei may occur in the WD patients.The functional activity of nuclei may be different between hepatic and cerebral type patients.Damage to the activity of neurons in the putamen and thalamus may correlate with psychiatric symptoms in WD patients.