Objective To investigate closure of different Fisher grade patients with subarachnoid product volume and the degree of cerebral vasospasm aneurysm clipping.Methods Patients with subarachnoid hemorrhage (SAH) were selected from January 2014 to January 2015,including 20 patients with grade Ⅰ,25 patients with grade Ⅱ,and 15 patients with grade Ⅲ.They were divided into 20 patients with aneurysmal SAH(A group),18 patients without aneurysmal SAH (B group) and C group(22 cases).The operation effect was compared.Results C group as the standard,CT preoperative diagnosis had no statistically significant difference among A,B,C group (x2 =0.12,P ＞0.05).After treatment,the cerebral spasticity scores of A,B group were significantly decreased,and the level-Ⅰ and Ⅱ patients decreased more significantly compared with stage Ⅲ patients,the difference between the two groups was statistically significant(x2 =7.05,P ＜ 0.05).In group C,the blood flow velocity at the 7th and 13rd day significantly increased (x2 =7.05,P ＜ 0.05),and velocity of blood flow in group A and B Ⅰ and Ⅱ patients was significantly higher than that of grade Ⅲ patients (t =8.75,P ＜ 0.05).Conclusion For the patients with Fisher grade Ⅰ and Ⅱ,the increase in the volume of subarachnoid hemorrhage,cerebral vasospasm,and the effect of the patients with Ⅲ were more obvious.

Objective To investigate the changing trends in 4 continuous point prevalence surveys of healthcare-as-sociated infection(HAI)in a hospital. Methods The occurrence of HAI among all hospitalized patients were inves-tigated on a given day from 0:00 to 24:00 in May,2006,2008,2010,and 2012 respectively.Results A total of 4 497 patients should be investigated,4 387 (97.55% )patients were actually investigated;184 patients developed 209 times of HAI,point prevalence rate of HAI was 4.19% ,case prevalence rate was 4.76% . Trendχ2 test showed that HAI prevalence rate decreased gradually from 5.56% in 2006 to 2.76% in 2012(χ2= 14.07,P<0.001).The main infection site was lower respiratory tract (55.03% ),followed by urinary tract(14.83% )and upper respiratory tract(9.57% ).The overall usage rate of antimicrobial agents was 38.50% . Trendχ2 test showed that usage rate of antimicrobial agents decreased gradually from 2006 to 2012(χ2= 5.13,P= 0.023);pathogenic detection rate in pa-tients receiving therapeutic and therapeutic plus prophylactic antimicrobial use increased gradually(χ2= 40.81 ,P<0.001);single use of antimicrobial agents increased gradually(χ2= 23.86,P<0.001). The rate of arteriovenous in-tubation ,urinary catheterization ,respirator use,and venous intubation in patients with HAI was significantly higher than those without HAI (27.17% vs 9.80% ,35.33% vs 11.54% ,13.59% vs 4.33% ,84.78% vs 63.24%respectively ,all P<0.001). Conclusion Management of rational use of antimicrobial agents has been achieved re-markably,control of HAI improved continuously;but control of invasive procedure-associated infection need to be intensified,management of rational use of antimicrobial agents should be paid much attention.

Objective To study the value of discriminant analysis of motor unit potential(MUP) multi-parameters in differentiation of various sorts of MUP. MethodsFrom the biceps brachii, tibialis anterior and vastus medialis muscles,the duration, amplitude, area and the ratio of area to amplitude of the MUPs were obtained by use of the spike-wave triggered average technique in 10 patients with myopathic disease, 13 patients with neurogenic disease and 22 healthy adults. The data were subject to analysis of variance and discriminant analysis. ResultsThe differences among the three groups with regard to all the parameters of the MUPs recorded from all the three muscles were statistically significant ( P

Hepatic venous outflow obstruction is a vascular complication after pediatric liver transplantation.Endovascular treatment has become the first therapeutic option for hepatic venous outflow obstruction after pediatric liver transplantation.The progresses in endovascular treatment of hepatic venous outflow obstruction after pediatric liver transplantation were reviewed in this article.

China has the largest human biological and pathological tissue resource in terms of both quantity and diversity, whichis true for oral cancer as well. However, this unique and valuable resource did not yield expected results in clinical translational research.On one side, clinicians lack experience in identification of research topics, even with plenty of clinical specimens. On the otherside, researchers consider high impact publications as the top priority instead of clinical significance. The disalignment of the interestsbetween these two sides led to inefficient and inadequate communications and collaborations. To change the current situation, we needto encourage clinicians to raise questions and generate research topics from clinical practice and to actively participate in translationalresearch. Meanwhile, researchers need to understand basics of clinical medicine and design research projects with a focus on clinicalproblems. Closer collaborations between clinicians and researchers will help to identify new biomarkers of clinical significance throughsystematic clinical studies, which have the potential to translate into practical knowledge in diagnosis, treatment and prognosis of diseases.We can further evaluate and validate these translational results systematically by large-scale random clinical trials.

Objective To explore correlations between hepatitis B virus (HBV)genotyping,other clinical information and drug resistance mutations.Methods 358 cases of patients with chronic hepatitis B (CHB)were selected as subjects,and the resistance loci and genotypes were detected by using the polymerase chain reaction(PCR)-reverse dot blot technique.Clinical data,such as ser-um HBV DNA loads,serum levels of alanine aminotransferase(ALT)and hepatitis B virus e antigen(HBeAg),gender,age and length of nucleoside analogues use were collected.Results All samples were successfully amplified positive band.Type B(267 ca-ses)was the main HBV genotype,followed by type C(81 cases)and type D(10 cases).In the 31 1 cases of patients taking nucleoside analogues,269 cases were completely wild type.No drug resistance mutation was found in 47 cases of patients not taking medicine. The drug resistance mutations mainly occured in 204 and 180/204 site.There was no significant correlation between resistance mu-tations and gender,age,serum HBV DNA loads,genotype,serum levels of ALT and HBeAg(P >0.05).While the medication time was longer,the incidence of resistant mutants was greater(P <0.05).The 180 mutation had a certain correlation with 204 site mu-tation(P <0.05).Conclusion PCR-reverse dot blot technology can effectively detect the HBV genotype and mutations,which could effectively guide the clinical medication.

Objective The main economic qualities of natural triploid were evaluated and the result laid a foundation to culture new triploid variety of Dioscorea zingiberensis. Methods The ploidy level of series of D. zingiberensis from some areas of Yunnan Province were identified and the qualities of outputs per plantlet,propagation ratio,and diosgenin content etc. were measured. Results Five natural triploids were found and the qualities of the triploids all displayed diversity,output per plantlet 1 090.00—628.57 g,propagation ratio (ploidy) 72.43—29.43,dry substance ratio 36.95%—24.06%,and diosgenin content 4.40%—1.50%. The outputs of series 1 and series 3 exceed 1 000 g,the diosgenin content of series 2 reached to 4.40%,and series 3 displayed excellent quality of outputs per plantlet,propagation ratio,and diosgenin content. The synthetical index is maximum. Conclusion The series comprising good quality are obtained by the discovery of triploid and evaluation of qualities. The triploid variety is to be created with judgment and selection of progeny.

Objective: To explore the relationship between serum levels of carbohydrate antigen 125 (CA125) and hemodynamic changes in patients with chronic heart failure (CHF).
Methods: A total of 110 CHF patients with clear diagnosis, admitted in our hospital from 2013-01 to 2014-06 were studied. According to NYHA classiifcation, the CHF patients were divided into 3 groups: NYHA I-II group,n=40, NYHA III group,n=38 and NYHA IV group,n=32. There was a Control group including 40 subjects from healthy physical examination. The levels of CA125, B-type natriuretic peptide (BNP), left ventricular ejection fraction (LVEF) were recorded at admission, discharge and 3 months follow-up period; the relationship between CA125, BNP and LEVF were studied in CHF patients.
Results:①In CHF patients, the levels of CA125 and BNP increased with NYHA classification by NYHA I-II group > NYHA III > group > NYHA IV group accordingly,P<0.05; while LVEF decreased with the elevated NYHA classiifcation, allP<0.05.②Correlation study indicated that CA125 positively related to BNP (r=0.64,P<0.001), LAD (r=0.28,P<0.01), LVEDD (r=0.35,P<0.001), and negatively related to LEVF (r=-0.63,P<0.001).③After treatment, the serum levels of CA125 in NYHA III group and NYHA IV group were obviously decreased,P<0.05.④There were 27 patients died at in-hospital and follow-up period, their levels of CA125 were (110.3 ± 40.2) before treatment and (67.1 ± 20.2) after treatment; for 83 survivors, their levels of CA125 were (66.7 ± 30.6) before treatment and (50.3 ± 24.5) after treatment, allP<0.05.
Conclusion: Serum level of CA125 is positively related to the severity of CHF, it might be used as a serum predictor for prognosis in CHF patients.

Contemporary social developments and changes have greatly influenced university students’thought and psychology,for the universities of ideological and political education not only brings the opportunity,but also brings the challenges.The universities of ideological and political education work to strengthen mental health education,in practice,can improve the universities of ideological and political education work more targeted and effective.

The position,length,size,and the site and pattern of ramification of the innominate arteries and common carotid arteries were studied in 76 specimens(♂44,♀32)of one- year-old to three-year-old Chinese infants. 1.The position relation between the innominate artery and trachea may be divided into three patterns:type Ⅰ:2 infants(2.63?1.8%),type Ⅱ:33 infants(43.42? 5.72%),type Ⅲ:41 infants(53.95?5.71%). 2.In 76 specimens the ramification region of innominate arteries of the Chinese infants were studied,among which in 57 specimens(75.0?4.96%)the site of ramifi- cation of innominate arteries is situated above the upper border of the sternoclavicular joint,and in 19 specimens(25.0?4.96%)behind the sternoclavicular joint.The region of ramification is higher in position in the infant than in the adult. 3.The average length of innominate arteries of all 76 specimens of 3 age groups: one,two,and three years old,is 20.7?0.42mm,19.2?0.56mm and 21.8?0.94mm respectively.The average circumference is 19.8?0.64mm,20.3?0.21mm and 21.0? 0.51mm respectively. 4.In 63 cases(82.9?4.32%)the innominate artery arises from the aortic arch by itself,in the other 13(17.1?4.32%)it arises from a common trunk,which is formed by the union of the innominate artery with the left common carotid artery.Among the 76 specimens,71 cases did not give off branches on its way,but in 4 specimens(5.26? 2.56%)the thyroidea ima is branched off directly from the innominate artery on its way,while in 1 specimen(1.32?1.31%)it arises from the common trunk. 5.The average site of ramification of the common carotid artery of the 76 Chinese infants is situated at the level of npper border of the intervertebral disc between the third and the fourth cervical vertebrae,but it is different on both sides:37 cases(48.7? 5.73%)with a higher level on the left,14 cases(18.4?4.44%)with.a higher level on the right side,25 cases(32.9?5.38%)with both sides on the same level. 6.The ramification angle of the common carotid arteries of the 76 infants fall into three patterns:60 sides(39.5?3.96%)belong to the acute angle pattern,40 sides(26.3? 3.57%)belong to the arcform pattern and 52 sides(34.2?84%)belong to the in- termediate pattern.The acute angle pattern is more common than the arcform pattern in the infants. 7.The average length of common carotid arteries of all 76 specimens of the three age-groups in one-、two-、three-year-old Chinese infants was 42.9?1.55mm,45.7?0.57 mm and 47.8?1.50mm on the right side and 59.0?1.10 mm,56.7?2.99mm and 64.9?1.62mm on the left side respectively,but the average circumference was 14.8 ?0.77mm,16.3?0.14mm and 16.6?0.19mm on the right side and 15.6?0.61 mm, 16.6?0.13mm and 17.3?0.40mm on the left side respectively.