Objective:To investigate the expression of survivin and VEGF in cholangiocarcinoma and explore its relation with clinicobiological behavior. Methods: Immunohistochemical techique was adopted in detecting the survivin and VEGF expression in cholangiocarcinoma and normal bile duct tissues. 51 samples of cholangiocarcinoma were collected in Qilu Hospital of Shandong University from January 2003 to December 2005 , and served as the test group, and 10 samples of normalbile duct tissues as control group. Results: 1)The expression rate of survivin in cholangiocarcinoma was 74.5% , whereas in normal bile duct tissue was 0% , the difference was markablely significant (P0.05). 2)The expression rate of VEGF in cholangiocarcinoma was 80.4% , whereas in normal bile duct tissue was 10% , the difference was significant (P0.05) . VEGF expression of the patients with lymph node metastasis was higher than that of group without lymph node metastasis. 3)The expression of both survivin and VEGF, and was 70.6% in the same sample. Conclusion: Survivin and VEGF may play an important role in tumor genesis, tumor progression, infiltration and metastasis of cholangiocarcinoma, and with intimate relation in angiogenesis.

Objective To explore the clinical effect of a small incision and traditional open resection for thyroid resection.Methods 72 patients with benign thyroid disease were randomly divided into small incision thyroid resection group and traditional open thyroid resection group,including small incision of 30 cases of thyroid resection group,the traditional open 42 patients with thyroid resection group,compared two sets of patients at the time of the operation,and postoperative peri-operative bleeding and postoperative pain score led flow,length of hospital stay,3months after operation neck feel and incision satisfaction.Results The two groups of patients were performed successfully,compared with traditional open thyroid resection small incision thyroid resection had longer time,the operation of less peri-operative bleeding and postoperative lead flow and postoperative pain score less light,the length of time was short,compared the indicators had statistically significant difference(all P ＜ 0.05).After 3 months follow-up results showed that patients in traditional open thyroid resection group appeared paresthesia,difficulty swallowing the neck was heavier,and degree of small incision thyroid after resection,incision,the above-mentioned higher scores satisfaction index comparative differences were statistically significant(all P ＜ 0.05).Conclusion Resection of small incision with thyroid small trauma,had quick recovery,less complications,in addition,postoperative pain advantages lighter,and incision was beautiful,and it was a safe and effective operation method.

Lymphoma is a group of malignancies that affect lymphatic system of the body,and often originates from the abnormal cloned proliferation of B,T or NK cell lineage.It contains mainly two categories:Hodgkin lymphoma (HL) and non-Hodgkin lymphoma (NHL).The treatment options consist of chemotherapy,radiotherapy,immunotherapy and radioimmunotherapy,et al.It has been demonstrated that lymphomas are typically radiosensitive tumors and undergo apoptosis readily after radiation therapy(RT).RT produces very high local complete response rate for all lymphoma subtypes.This review will mainly focus on the role of RT in the treatment of aggressive lymphoma,indolent lymphoma,extranodal lymphoma and HL,as well as the application of modem RT techniques.

Neovascularization is the fundamental process during tumorigenesis and tumor malignant progression. According the traditional neovascularization theory, tumor vasculatures are mainly developed through angiogenesis by sprouting from preexisting ves-sels and vasculogenesis via recruitment of endothelial progenitor cells from the bone marrow, and the endothelial-dependant vessels are the only way that provides tumor with blood. However, more and more studies indicate that tumor microcirculation network is heteroge-neous and cancer stem cells (CSCs) play an important role during tumor neovascularization. This review highlights the contribution of CSCs to tumor microcirculation modes and the potential anti-angiogenesis targets. Furthermore, this review presents insights for perti-nent studies in the future.

Objective To evaluate the efficacy of testosterone supplementation for partial androgen deficiency in middle-aged men. Methods This prospective,double-blind,placebo-controlled,randomized clinical trial was conducted in our hospital.A total of 179 patients (mean age,41.8 years) were randomly divided into study group (n=136) and control group (n=43).The 136 patients in study group received andriol (80 mg,twice a day) for 3 months;while the 43 patients in control group received no special treatment.The changes of partial androgen deficiency in aging male (PADAM) scores,serum total testosterone (TT),free-T (fT) and relevant sexual hormone levels were assessed before and after treatment. Results In study group the PADAM scores at 1 and 3 months after treatment were significantly improved compare with those before treatment (P0.05). Conclusions Partial androgen deficiency in middle-aged men is clinically exist and testosterone supplementation for this condition is safe and effective.

Objective:HPLC was used for the determination of galangin and kaempferol of Rhizoma Alpiniae Officinarum.Methods:The column was phenomenex Gemini C 18 (5?,250mm?4.6mm);the mobile phases were methanol-0.4% phosphoric acid(60:40);the detection wavelength was 360 nm;the flow rate was at 1.0 ml/min and the column temperature was at 30?C respectively.Results:Galangin showed a good linear relationship in the range of 0.668-6.680?g,r=0.9996,and the average recovery was 102.30%with RSD=1.73%(n=6);Kaempferol also showed a good linear relationship in the range of 15.5-155.0ng,r=0.9995,and the average recovery was 97.31%with RSD 1.84%(n=6).Conclusion:This method was simple,accurate,reliable and sensitive and it can be used as a quality control standards for Rhizoma Alpiniae Officinarum.

BACKGROUND:In recent years, the spinal internal fixation technology has made rapid development based on biomechanics and material sciences.
OBJECTIVE: To review the biomechanical characteristics of the lumbosacral spine and the application of various internal fixation materials in the reconstruction of spinal stability after lumbosacral spinal tuberculosis.
METHODS:A computer-based search of Medline and Chinese Journal Ful-Text Database was performed for relevant articles using the keyword of “lumbosacral spinal tuberculosis, biomaterials materials, fixation” in English and Chinese, respectively.
RESULTS AND CONCLUSION: Rigid internal fixation is a conventional treatment for lumbosacral tuberculosis, which improves the spinal alignment and stability during the spinal reconstruction. Metalic materials such as stainless steel, titanium and titanium aloys have been widely used in rigid internal fixation, but metal sedimentation, non-transparency, stress shielding and osteoporosis after internal fixation impact the fusion effects and imaging observation. Absorbable materials as newly-developing materials have good biocompatibility and biodegradability in orthopedic internal fixation. To select the appropriate material for internal fixation, the biomechanical properties of internal fixation materials wil be investigated according to the degree of vertebral damage and lumbosacral stability.

Objective To assess cerebrovascular reserve ( CVR) function in patients with internal border zone infarction(IBZI) induced by severe middle cerebral artery (MCA) stenosis, and investigate the impact on progression and outcome of the disease .Methods A total of 84 patients with unilateral severe MCA stenosis were selected . Hypercapnia was induced by holding breath .The change of blood flow velocity in MCA was measured by transcranial Doppler ( TCD ) to calculate CVR .According to CVR , patients were divided into impaired regional CVR group ( CVR <10%) and normal CVR group ( CVR ≥10%) .The NIHSS was used to evaluate neurological function in both groups within 14 d, and mRS was used to evaluate acute stage outcome of the patients at discharge .All the patients were followed-up for 6 months, the incidences of recurrence , complications and mortality in the two groups were analyzed .Results The incidence of progressive cerebral infarction in the impaired regional CVR group (67.39%) was significantly higher than that in the normal CVR group (42.11%) ( P<0.05).The impaired regional CVR group showed higher proportion of patients with poor clinical outcome at discharge ( mRS≥3 ) (63.04%)compared to the normal CVR group (36.84%) (P<0.05).In the followed-up 6 months, the incidences of recurrence and complications were 34.78% and 45.65% respectively in the impaired regional CVR group , they were significantly higher than that in normal CVR group (15.79%,23.68%)(P<0.05).The overall mortality rates did not differ significantly between the two groups ( P>0.05 ) .Conclusion Impaired regional CVR may be predictive of subsequent progressive cerebral infarction and poor clinical outcomes in patients with IBZI induced by severe MCA stenosis .

Objective To know normal reference value of heart-type fatty acid-binding protein (H-FABP) in serum. Methods Serum H-FABP levels of 126 healthy individuals were measured by ELISA. Levels of MYO, cTnI and CK-MB were assayed in the same time as control. The normal threshold value was calculated as mean value?2s. Results The normal concentration of H-FABP in serum was 3 79?3 52?g/L and the difference in age and gender wasn’t significant. Conclusions The normal reference value of serum H-FABP is 0～10 83?g/L.

As an important measure to reduce casualties and prevent secondary pollution, decontamination is an impor-tant link in the process of emergency response during chemical accidents.The decontamination effect is closely related to decontamination technology and equipment.Decontamination agent selection and development are an important part of a decontamination technology.In this paper, the development and use of cleaning agents, such as alkaline, oxidation and chlorination, adsorption (degradation), metal oxide and oxygen acid salt, chemical compounds, biological (enzymatic), and individual disinfection package, light decontamination equipment, multifunctional integrated large-scale decontamination equipment at home or abroad, are reviewed.By laying bare the gap between China and advanced countries in the related field, we hope to raise the concern of relevant professional counterparts and promote the development of domestic decontami-nation technology and equipment with decontaminant agents at the core.