1.The performance verification of high-sensitive C reactive protein test reagent
Zhiying HU ; Mei HU ; Man ZHANG
International Journal of Laboratory Medicine 2014;(17):2313-2314
Objective To verify the accuracy,repeatability and consistency with contracting reagent of the high-sensitive C reac-tive protein(hs-CRP)test by using the independently developed reagents.Methods 150 samples were collected,including 130 ser-um samples and 20 plasma samples.4 times of the relative difference of mean value was used as the detection limits,and the regres-sion analysis was performed after excluding 5 sample outliers,then calculated the expected bias at medical decision level with the 95% confidence interval,in order to judge whether the bias was within the allowable range.Results The recovery rate of hs-CRP test reagent were within the allowable range which was 90% -110%.The hs-CRP testing results of plasma and serum samples from the same source were consistent,and the expected bias of medical decision level was in the permissible range.Conclusion The developed reagent used in hs-CRP test have good accuracy,repeatability,and highly consistency with control reagent.
2.Expression of placental vascular growth factor in pregnancy-induced hypertension
Zhiying HU ; Marong FANG ; Yiping WANG
Chinese Journal of Perinatal Medicine 1998;0(02):-
Objective To investigate the expression of vascular growth factors including vascular endothelial growth factor(VEGF), basic fibroblast growth factor(bFGF) and platelet-derived growth factor (p-DGF) in placentae of pregnancy-induced hypertension(PIH) patients and their significance. Methods Placental tissues of 15 normal gravidas and 19 PIH women were collected. The expression of vascular growth factors were detected by immunohistochemistry (PAP) combined with the analysis of computer microphotography. Results The average grey value difference (GVD) of VEGF was 61.6?3.7 in moderate and severe PIH group, which was significantly lower than that of the normal control group (71.1?5.8) and mild PIH (70.9?8.9, P
3.Progress in oral drug-loaded nano-system
Zhiying DING ; Yue ZHANG ; Yaru DIAO ; Jihong CHI ; Yulin HU
Chinese Journal of Biochemical Pharmaceutics 2016;36(6):30-32
Oral drug-loaded nano-system include nano-gel drug delivery system, nano-suspension drug delivery system, nano-particle drug delivery system, liposomes drug delivery system, nano-micelles drug delivery system, alcohol liposoms,nano-framework drug delivery system, nano-emulsions drug delivery system, nano-self assembly drug delivery system.These nano-drug delivery systems can serve as multi-functional drug carriers.They may significantly improve the physicochemical and stabilization and biological properties of the free drug, enhance the therapeutic efficiency and reduce toxic side effects.This paper reviews the recent research progress in oral drug-loaded nano-systems.
4.Epidemiological survey of nosocomial infection of imipenem-resistant Klebsiella pneumoniae in Beijing Shijitan Hospital
Duo YANG ; Na LI ; Xuli XIN ; Zhiying HU
Chongqing Medicine 2017;46(7):941-942,945
Objective To study the homology relationship among clinically isolated imipenem-resistant klebsiella pneumoniae strains and possible transmission route of drug resistant strains to provide a basis for blocking the transmission of this kind of bacteria in clinc.Methods Clinical samples collection and bacterial culture were routinely conducted,meanwhile the hand surface of ICU medical staffs and the surfaces of patien's bedside objects were performed the sample collection for conducting the bacterial count and culture.The bacterial identification and drug susceptibility test were performed by the VITEK-2 COMPACT system.The bacterial homology detection adopted the ERIC-rep-PCR method.Results Totally 642 strains of imipenem-resistant Klebsiella pneumoniae were clinically isolated from January 2011 to December 2013.Among them,389 strains(60.59%) were derived from ICU.72 strains (11.21%) fsrom the respiratory department,53 strains (8.26%) from the neurology department,41 strains (6.39 %) from the cardiovascular department,31 strains (4.83%) from the surgical department,11 strains (1.71%) from the gastroenterology department,5 strains (0.78 %) from the renal department,17 strains (2.65 %) from the emergency department and 23 strains(3.58%) from the other departments.One strain of imipenem-resistant Klebsiella pneumoniae was isolated from the IGU object surface.Among 642 clinical bacterial strains,there were 8 main clones,these 8 clone strains added up to 394 strains,which accounted for 61.37% of whole bacterial strains,the other 248 strains were single clones.Conclusion The monoclonal prevalence of imipenem-resistant klebsiella pneumonia in this hospital actually exists.The contact transmission may be the possible transmission route of this kind of infection.
5.Clinical analysis of maternal and infant adverse outcomes with group B streptococcus colonization during the late pregnancy
Yuanye WU ; Jianting MA ; Zhiying HU ; Lili TU
Chinese Journal of Postgraduates of Medicine 2021;44(6):568-570
Objective:To investigatethe maternal and infant adverse outcomes of group B streptococcus (GBS) colonization during the late pregnancy.Methods:A total of 567 pregnant women who underwent obstetrics and gynecology examination in Yuyao People′s Hospital from December 2018 to June 2019 were selected for the study. Internal vaginal secretions of all the pregnant women were extracted from the lower 1/3 of the vagina for bacterial culture, and GBS culture and screening were performed on pregnant women. According to the results of GBS screening, pregnant women were divided into GBS negative group (8 cases) and GBS positive group (559 cases). The general condition and pregnancy outcomes of pregnant women were compared between the two groups, and the correlation between pregnancy outcomes and streptococcus colonization was analyzed.Results:There were no statistically significant differences in age, gestational weeks, number of births, number of pregnancies, history of abortion, residence area and ethnicity between the two groups ( P>0.05). The incidence of premature rupture of membranes and fetal distress in GBS positive group were higher than those in GBS negative group:5/8 vs. 7.69%(43/559), 3/8 vs. 5.01%(28/559), and the differences were statistically significant ( P<0.05). Spearman single factor correlation analysis showed that GBS colonization in pregnant women were positively correlated with premature rupture of membranes and fetal distress ( r = 0.632, 0.573, P<0.05). Conclusions:GBS colonization in late pregnancy is closely related to pregnancy outcomes, and strengthening GBS screening has great significance to reduce adverse pregnancy outcomes.
6.The assessment and analysis of nutritional status of 1200 inhospital patients
Ping SHEN ; Zhiying ZOU ; Nanhai PENG ; Guilan LU ; Jihong ZHONG ; Linfeng HU ; Yahong LIU ; Gui ZHANG
Parenteral & Enteral Nutrition 1997;0(01):-
Objective:To assess incidence of malnutrition and malnutrition risk of six department patients.Methods:The information of 1 200 patients were collected,200 in each of 6 departments in our hospital.Nutrition status was assessed according to Nutrition Risk Screening(NRS)published by ESPEN in 2001.Results:The incidence of malnutrition and malnutrition risk varied from 7.5% to 59% and 36% to 72% respectively in different department.Conclusion:The incidence of malnutrition is closely related to the kind and severity of the disease.It is nessissary to assess the nutrition status of high risk patients in time.NRS can be used simply and fastly in most inhospital patients.
7.IMMUNOHISTOCHEMICAL RESEARCH ON CGRP AND SP IN THECANINE MAIN SUPERFICIAL CARDIAC PLEXUS
Zhiying ZHAO ; Dongliang MA ; Yueying LI ; Haitao HU ; Weixi WANG ; Bingxiang YUAN
Acta Anatomica Sinica 2002;0(05):-
Objective To investigate the chemical properties of superficial cardiac neurons. Methods By means of immunohistochemical ABC technique,the study was performed concerning the distribution of calcitonin gene-related peptide(CGRP) and substance P(SP) in the canine main cardiac superficial plexus. Results CGRP-immunoreactive(IR) neurons were found in every plexus,but SP-IR neurons could be observed only in dorsal atria plexus(DAP),inter atria plexus(IAP) and aorta-pulmonary plexus(A-PP).The shape and size of CGRP-IR and SP-IR neurons were similar.The comparative study on atria and ventricles indicated that CGRP-IR and SP-IR neurons in atria were more than those in ventricles.Numerous CGRP-IR,SP-IR nerve fibers could be observed in each fat pats and intermyocardiocytes.These nerve fibers were usually situated near blood vessels,or were attached to vessel wall.Somewhat CGRP-IR and SP-IR nerve fibers were connected with myocardiocytes in some regions.Conclusion The results indicated that actually existed two peptides in canine cardiac superficial plexus.These implied that the regulations of the two kinds of peptidergic neurons to atria and ventricle were different.The two kinds of neurons mentioned above were likely to perform different or similar functions in canine heart.CGRP and SP could possibly modulate the activities of myocardiocytes and vessels of heart directly.
8.Application of Fah knockout mice in the hepatocyte transplantation and the pathological changes
Baoliang SU ; Changcheng LIU ; Shuai LI ; Ke YANG ; Zhiying HE ; Yiping HU ; Xin WANG
Journal of China Pharmaceutical University 2009;40(6):553-558
Aim: To investigate the pathological changes in NTBC[2-(2-nitro-4-trifluoro-methyl-benzoyl) -1,3 cy-clohexanedione]-induced hepatic injury in mice and in the repopulation of adult hepatocytes in Fah~(-/-) mouse. Methods: Autogenous hepatic injuries in Fah~(-/-) mice were induced by the treatment of NTBC. Injection of hepatocytes obtained from wild-type mice to spleen were transplanted into the Fah~(-/-) mice. Then, changes to body weight and the likelihood of the transplanted Fah~(-/-) mice, and hepatic immunohistochemistry were ob-served. In addition, pathological changes to liver damage induced by NTBC treatment were analyzed under HE-staining microscopy and electron microscopy. Results: The surviving Fah~(-/-) mice subjected to hepatocyte trans-plantation were found to be healthy and in stable body weight. liver repopulation reached to 90% in the 8th week. Repopulating hepatocytes caused no alteration to histological structure of the recipient liver, and subacute hepatic injury occurred in the Fah~(-/-) mice after NTBC treatment. Electronic microscopy observations indicated that necrosis in the hepatocytes occurred at early stage and that apoptosis gradually appeared. It was also shown both necrosis and apoptosis co-existed in the same samples of interest at the following stages of the induced liver injury. Conclusion: Transplanted hepatocytes proliferated in Fah~(-/-) mice allow 90% of the hepatocytic repopula- tion. Repopulation renders normal hepatic function and structure in the recipient Fah~(-/-) mice, as a model of liver repopulation, could be applicable in study of stem cell derived hepatic cells in transplantation assay.
9.Long-term adherence to chemoprevention agents among women at high risk of breast cancer
Ning MA ; Chongzhu HU ; Enqing WANG ; Ruixue YUE ; Huan LI ; Hui ZHANG ; Wenjuan ZHANG ; Zhiying BIAN
Chinese Journal of General Practitioners 2017;16(4):300-303
One hundred and eighty three women with high risk of breast cancer identified in Baoding First Central Hospital from June 2007 to June 2014,were enrolled in the study.In these 183 cases,41 received preventive therapy (22.4%),142 did not.Factors associated with acceptance of chemoprevention included older age,menopause,comorbidities,lower level education,being married,higher BMI,having high level hyperplasia of ductal epthelia and counseling to breast specialists (P < O.05);whilenot informed by doctors was the main reason for failed taking preventive agents(95.1%,135/142).Thirteen of 41 women receiving chemoprevention discontinued the therapy (31.7%);no-insurance,no side-effects and higher BMI were related to the discontinuing (P < 0.05).The 1-,2-,3-,and 5-year adherence rates of chemoprevention was 86.1% (31/36),65.4% (17/26),59.1% (13/22),8/13 and 5/10,respectively.The results show that chemoprevention rate is low among Chinese women at high risk of breast cancer,and the long-term adherence to preventive therapy is poor.Corresponding measures should be taken to strengthen the chemoprevention of breast cancer.
10.Mutation screening in taperin gene in Chinese with prelingual nonsyndromic hearing impairment.
Qin WANG ; Zhiying NIE ; Yan DING ; Jie QING ; Ruosha LAI ; Dinghua XIE ; Peng HU
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2014;28(10):672-675
OBJECTIVE:
To screen and identify the frequency and characteristic of mutation in stereocilium-related gene Taperin of Chinese prelingual nonsyndromic hearing impairment with DNA microarray combined with PCR.
METHOD:
One hundred and thirty-four patients of prelingual nonsyndromic deafness and one hundred health individuals in China were investigated in this study. Genomic DNA was extracted from the patients and was subjected to DNA microarray to screen mutations in 4 most common genes. The samples that carried none of the common mutant alleles were subjected to PCR and sequenced to detect mutations in Taperin gene.
RESULT:
Ninteen out of one hundred and thirty-four patients of prelingual nonsyndromic deafness were detected carring common deafness gene with DNA microarray. Taperin gene were detected in one hundred and fifteen patients with PCR. A187S was detected in Taperin as hetrozygous state in 2 patients and their unaffected members of their family. It occurred at the evolutionary conservation of the amino acids of taperin according to alignment analysis. Two polymorphism, 157C>T and 318C>T, were found in the patients and the control group.
CONCLUSION
A novel Taperin mutation, A187S was detected in Chinese patients with prelingual nonsyndromic hearing loss, which may be relevant to hearing loss. Two polymorphism, 157C>T and 318C>T, were found in Chinese in our research. The carrier frequency for Taperin mutation is about 1.74% of prelingual nonsyndromic deafness in Chinese patients.
Adolescent
;
Adult
;
Asian Continental Ancestry Group
;
genetics
;
Child
;
Child, Preschool
;
China
;
Deafness
;
genetics
;
Female
;
Humans
;
Male
;
Mutation
;
Proteins
;
genetics
;
Young Adult