The meaning,the principle and concrete methods of researching and establishment of the basic request university mathematics course teaching of medicine profession is discussed. By the analysis of the present constitution of mathematics course in medical education,the author points out the establishment such basic request has certain theoretical value and practical meaning.He has also discussed some principle and the methods to realize this value and meaning.

In order to investigate the therapeutic effect of traditional chinese medicine "Re Du Qing" on silicosis, the cultured and purifiel peritoneal macrophages in five groups obtained from mice were observed dynamically with cytochemical me- thods and scanning electron microscope. The results showed that the survival times of cell cultures were 2-3 weeks, 24-48, 72, 48-72 and 48-72 hr in normal, silica, "Re Du Qing", P204 and joint action group respectively in vitro. The characteristics of cell morphology with a series of cell surface ultrastructural changes in different times of culturd and the stages of various function were different in five groups. The cell surface ultrastructural changes of "Re Du Qing" group were similar to the normal group. The macrophages in the silica group phagocytosed silica dusts rapidly died and broken down much earlier than the cells in "Re Du Qing" group. The cell surface ultrastructural changes in P204 group were less than that of the cells in silica group, whereas the eell surface ultrastructural changes in joint action group were between the cells in "Re Du Qing" and P204 groups. The activities of intracellular acid phosphatase (AcP) and succinic dehydrogenase (SDH) in silica group were also much lower than that of "Re Du Qing" one. This study suggested that traditional chinese medicine "Re Du Qing" is evidently more effective on therapy of experimental silicosis.

OBJECTIVE: To understand the current situation of clinical drug use and to inspect the rationality of drug use and survey indices. METHODS: Twenty common diseases(1 530 cases ) treated in this hospital in 1995 were selected. Patierits were divided into three groups according to the sources of expenses: free of charge, self - expense, public - expense or into cases of internal medical and surgical departments. Using selected drug use indicators(SDUI) and self - designed indices, clinical drug use were surveyed and analyzed. RESULTS: There were no significant differences between each group jn main indices. It showed that irrational drug use was only different in degree. CONCLUSION: ①The indices adopted are practicable and rational for surveying and studying the rational drug use objectively. ②In any health care system and in any disease, the irrational drug use was serious.

Objective To study the expression and effect of macrophage inflammatory protein-2(MIP-2) in liver injury induced by ischemia/reperfusion(I/R). Methods Thirty-two rats were randomly divided into 4 groups(8 rats in each group):false operation (control) group and 3, 9, 24 hours reperfusion group.The expression of MIP-2 mRNA in hepatic tissue, MIP-2 protein in plasma, the neutrophil infiltration in liver tissue and serum ALT were measured. Results The expression of MIP-2 mRNA in the ischemic tissue was significantly higher than that in nonischemic tissue (P

Objective To investigate the clinical value of double contrast-enhanced ultrasonography (DCUS) for the diagnosis of gastric cancer.Methods A retrospective analysis was performed to review the DCUS data of 26 patients which were diagnosed as gastric cancer by pathology in the third affiliated hospital of Kunming Medical University from October 2014 to July 2015.The analysis results were compared with postoperative pathology to get accuracy rates.Results The located accuracy,qualitative accuracy and accuracy at T phase of DUCS was 100％ (26/26),100％ (26/26) and 88.5％ (23/26),respectively.Compared with color doppler flow imaging (CDFI),the qualitative accuracy of DUCS was much higher (P ＜ 0.05).The accuracy of DUCS at T phase was higher than that of CDFI with no statistical significance (P ＞ 0.05) Conclusion DUCS has an important application value in the diagnosis of gastric cancer.

This study aimed at evaluating the quality of the precise powder decoction pieces (PPDP) of L.japonicae Flos (LJF) compared with the traditional commercial slices with chemical fingerprint methods and DNA molecular identification technology.Different specifications of PPDP were prepared,their dry extract contents were in contrast with that of commercial slices.The three batches of commercial slices were collected,and the content uniformity,fingerprint and similarity evaluation before and after the mixing and pulverization were studied by HPLC-DAD and DNA sequence alignment.As a result,the paste rate of PPDP was slightly higher than that of the traditional commercial slices.The dissolution of chlorogenic acid of PPDP was higher than that of the traditional commercial slices.RSD of inter-assay dissolutions of chlorogenic acid of commercial slices was 11.93％,which was reduced to 8.29％ after mixing and preparing into PPDP.The fingerprint showed that the slimilarity of the fringerprint of the mixed and powdered LJF was elevated with 7 common peaks.All the common peaks were increased at different levels.In conclusion,compared with traditional commercial slices of LJF,PPDP apparently improved the dissolution rate and the quality uniformity,indicating that the boiled powder of CRP obviously presented vantages in clinic.

Periodontitis is a chronic inflammatory and immune reactive disease induced by the subgingival biofilm.The therapeutic effect for susceptible patients is often unsatisfactory due to excessive inflammatory response and oxidative stress.Sinensetin(Sin)is a nature polymethoxylated flavonoid with anti-inflammatory and antioxidant activities.Our study aimed to explore the beneficial effect of Sin on periodontitis and the specific molecular mechanisms.We found that Sin attenuated oxidative stress and inflammatory levels of periodontal ligament cells(PDLCs)under inflammatory conditions.Administered Sin to rats with ligation-induced periodontitis models exhibited a protective effect against periodontitis in vivo.By molecular docking,we identified Bach1 as a strong binding target of Sin,and this binding was further verified by cellular thermal displacement assay and immunofluorescence assays.Chromatin immunoprecipitation-quantitative polymerase chain reaction results also revealed that Sin obstructed the binding of Bach1 to the HMOX1 promoter,subsequently upregulating the expression of the key antioxidant factor HO-1.Further functional experiments with Bach1 knocked down and overexpressed verified Bach1 as a key target for Sin to exert its antioxidant effects.Additionally,we demonstrated that Sin prompted the reduction of Bach1 by potentiating the ubiquitination degradation of Bach1,thereby inducing HO-1 expression and inhibiting oxidative stress.Overall,Sin could be a promising drug candidate for the treatment of periodontitis by targeting binding to Bach1.

Nannochloropsis has been considered as a promising feedstock for biodiesel production in recent years. To improve its lipid productivity, heavy-ion irradiation mutagenesis, an effectively breeding method used in plants and microorganisms was applied in Nannochloropsis oceanica OZ-1. After large-scale screening using Imaging-PAM and microplate-reader, two mutants (HP-1 and HP-2) with higher growth rate were isolated from the wild type N. oceanica. Subsequently analysis showed that after 18 days of cultivation biomass accumulation of the HP-1 and HP-2 mutant was increased by 18% and 26% respectively compare to the wild type. Total lipid productivity of the HP-1 and HP-2 mutant was 295 mg/(L x d) and 275 mg/(L x d), respectively, whereas that of the wild type was 247 mg/(L x d). Both mutants showed significantly advantage over their wild type concerning biomass accumulation and lipid productivity.
Heavy Ions ; Lipids ; biosynthesis ; Microalgae ; growth & development ; metabolism ; radiation effects ; Mutagenesis ; genetics ; Mutation ; genetics ; Stramenopiles ; genetics ; metabolism ; radiation effects

Taking the application of logistics robots in intravenous drug distribution at our hospital as an example, this paper discussed the application effect of hospital logistics robots in actual operation, and discussed how to use data analysis to carry out a comprehensive scientific evaluation of the work effect of hospital logistics robots. The results showed that the hospital logistics robots can take charge of the daily heavy workload by using of unmanned technology. And at the same time, the transportation of medical materials by such means ensures the safety of transportation. It can also realize the whole process monitoring and closed-looping of transportation information. Analysis of operational data proves that the use of hospital logistics robots conforms to the expectation of higher quality requirements in hospital logistics, thus strengthening and improving the level of modern management of hospital logistics.

OBJECTIVE: Exploring a method of rhinoplasty and septoplasty to get better nasal function and aesthetic effect. METHOD: 1) Using endoscopic rhinoplasty and septoplasty, separating the joint of quadrangular cartilage and the bones around it, resecting vertical and horizontal strip of the deviated septal cartilage and fracturing the deviated septal bones and reconstructing them with three layers (mucosa-cartilage or bone-mucosa), positing the reconstructed septum in the middle between two lateral walls of nasal cavity, proper space between septum and turbinates was maintained. 2) The caudal part of quadrangular cartilage was resected and placed in the columella pocket between the two medial parts of the alar cartilage and the supporting function of the septal cartilage was maintained. RESULT: Compared with traditional nasal septal reconstruction surgery, our method got better functional and aesthetic outcome. CONCLUSION The anatomic three layers were preserved in our method, thus it is better than the traditional methods. The caudal septal cartilage should be used as the perfect material in rhinoplasty.
Adolescent ; Adult ; Aged ; Endoscopy ; Female ; Humans ; Male ; Middle Aged ; Nasal Septum ; surgery ; Rhinoplasty ; methods ; Young Adult