From July of 1989 to January of 1991,we performed surgical operations on 20 cases with intractable epilepsy.The preoperative localization of epileptogenic foci was based on a comprehensive evaluation of patients'seizure history,scalp EEG,topographic EEG display, cranial electrode recording,CT and neuropsychological investigations.Then,we selected an adequate surgical treatment,including 1 case of hemispherectomy,10 cases of anterior tem- poral lobectomy and 8 cases of extratemporal resection of the epileptogenic focus.Detected by electrocorticography and electrical stimulation monitoring,16 postoperative cases(80%) had seizure free and 4 cases(20%)had a more than 50% reduction in seizure frequency. The localization of epileptogenic foci,and surgical method and effect were discussed in detail. The key of surgical selection is to exactly localize epileptogenic foci with a comprehensive evaluation of various recordings and studies.The intracranial electrode recording is important for the localization of an epileptogenic focus.

Objective To study the effect of intraoperative irrigation in management of traumatic rupture of left hemicolon.Methods 36 traumatic rupture of left hemicolon patients in our hospital were randomly divided into treatment group and control group.The treatment group received primary reparation and intraoperative irrigation of colon,and the control group received colostomy and repair.The therapeutic efficacy were compared between the two groups.Results There were no significant differences in complication rates and perioperative mortality between two groups( P ＞ 0.05 ),and the control group's the average hospitalization day and average hospitalization charge are more than the treatment group( P ＜ 0.01 ).Conclusion Intraoperative irrigation of colon and primary repair is a safe and feasible method for the traumatic rupture of left hemicolon.

Objective To explore the significance of combined detection of procalcitonin(PCT)and C reactive protein(CRP)for clinical use of antibacterial agents in patients with pulmonary disease.Methods 130 cases of patients with pulmonary disease,from October 2012 to October 2014,were selected and randomly dividend into the observation group and control group,65 cases in each group.In the observation group,the utilization of antibacterial agents was decided according to serum levels of PCT and CRP,while in the control group that was based on the white blood cell count.Observation indexes,such as utilization of antibacterial agents, curative efficiency,length of stay and so on,were compared between the two groups after treatment.Results The length of antibac-terial agents use in the observation group[(8.6±0.4)d]was longer than that in the control group[(1 6.2±2.3)d],had statistically significant difference(P <0.05 ).After treatment,there were no statistically significant differences in the white blood cell count, scores of pulmonary functional status,percentage of patients in aggravating stages and mortality between the two groups (P >0.05).While compared with the control group,the dual infection rate and length of stay decreased in the observation group,had sta-tistically significant difference(P <0.05).Conclusion Combined detection of PCT and CRP could guide the rational use of antibac-terial agents,effectively prevent the dual infection,and improve clinical curative effect.

The aim of this study was to refold the OvisAries leukocyte antigen (OLA) class Ⅰ protein with peptides derived from sheeppox virus (SPPV) to identify SPPV T cell epitopes. Two pairs of primers were designed based on the published sequence of a sheep major histocompatibility complex Ⅰ to amplify the heavy chain gene of OLA Ⅰ α-BSP and the light chain gene of OLA Ⅰ-β₂m. Both genes were cloned into a pET-28a(+) expression vector, respectively, and induced with ITPG for protein expression. After purification, the heavy chain and light chain proteins as well as peptides derived from SPPV were refolded at a ratio of 1:1:1 using a gradual dilution method. Molecular exclusion chromatography was used to test whether these peptides bind to the OLA Ⅰ complex. T-cell responses were assessed using freshly isolated PBMCs from immunized sheep through IFN-γ ELISPOT with peptides derived from SPPV protein. The results showed that the cloned heavy chain and light chain expressed sufficiently, with a molecular weight of 36.3 kDa and 16.7 kDa, respectively. The protein separated via a Superdex^TM 200 increase 10/300 GL column was collected and verified by SDS-PAGE after refolding. One SPPV CTL epitope was identified after combined refolding and functional studies based on T-cell epitopes derived from SPPV. An OLA Ⅰ/peptide complex was refolded correctly, which is necessary for the structural characterization. This study may contribute to the development of sheep vaccine based on peptides.
Animals ; Capripoxvirus ; Epitopes, T-Lymphocyte/genetics* ; Peptides/genetics* ; Poxviridae Infections ; Sheep ; Sheep Diseases