0.05),whereas the survival rate in each group of 160 and 320 ?mol/L was significantly higher than that in the control(P

Objective To establish a method for determining the soybean isoflavones and Monacolin K in fermented soybean product by high performance liquid chromatography(HPLC). Methods The soybean isoflavones and Monacolin K in fermented soybean were separated by HPLC with polaris C18 (2.0 mm?100 mm, 3?m)at 50℃.The mixed solution of water (containing 0.3%phosphatic acid) and acetonitrile was used as mobile phase for gradient elution at a rate 0.30ml/min. And the absorption was measured at 260 nm, 237 nm for the soybean isoflavones and Monacolin K, were determined of soybean isoflavones and Monacolin K in fermented soybean product. Results When the contents of soybean isoflavones and Monacolin K were in 0.2～45mg/L, there was a good linear relationship between absorption and content, by which the average recoveries were 88.9%～101.1% and relative standard deviation ranged between 0.89%～3.19%. Conclusion The method is accurate and reliable for quantitative analysis of the soybean isoflavones and Monacolin K in fermented soybean product.

Objective To summary our experiences on the clinical management of early complications caused by ureteroscopy and pneumatic lithotripsy. Methods From May 2002 to December 2007,totally 660 patients received ureteroscopy and pneumatic lithotripsy in our hospital,25 of them had surgical complications. Their clinical data were analyzed retrospectively. Results Among the 25 cases,22 patients had ureteral perforation,2 had ureteral disruptions,and 1 showed pelvic hematoma owing to ureteral perforation and injury to the ovary vein. Of the 22 cases of ureteral perforation,13 patients received lithotripsy and placement of a double J stent into the renal pelvis via the perforation site;and 4 patients underwent repair of the ureter. The other 5 of the 22 cases refused open surgery,and showed symptoms of urinary extravasation,loin pain,fever,or hematuria postoperatively;PCN was successfully completed in 3 of them but failed in the other 2,who underwent open surgery afterwards. For the 2 patients with ureteral disruptions,ureteroureteral anastomosis was performed. And in the case of pelvic hematoma,we carried out open surgery to ligate the bleeding vessels. All of the 25 patients were cured and discharged from hospital prosperously. The double J stent was withdrawn in 2 months after the operation,and 1-year follow-up showed no ureteral stenosis or obstruction by intravenous pyelography. ConclusionsPrimary management of ureteral injury by indwelling double J stent or percutaneous nephrostomy results in significantly deceased rate of re-operation. Conversion to open surgery timely can stop the incidence of other severe complications.

Objective To investigate the effect of nephrostomy on the infections after ureteroscopic holmium laser lithotripsy.Methods From March 2007 to May 2008,100 patients with unilateral upper ureteral calculi complicated with hydronephrosis were divided into control and experiment groups according the patients number (50 cases in each). Ureteroscopic holmium laser lithotripsy was carried out in the control group,while in the experiment group,nephrostomy was performed two days prior to ureteroscopic holmium laser lithotripsy. The clinical data including highest body temperature after the operation,recovery of body temperature,blood routine and urine routine,as well as hospital cost,were compared between the two groups. Results Compared with the control group,patients in the experiment group showed significantly lower highest temperature [(37.8?0.5) ℃ vs (38.6?0.5) ℃,t=-8.000,P=0.000],quicker recovery of body temperature [(1.6?0.2) d vs (2.8?0.4) d,t=-18.974,P=0.000],blood routine [(2.7?0.4) d vs (4.1?0.6) d,t=-13.728,P=0.000],and urine routine [(3.6?0.6) d vs (5.2?0.7) d,t=-12.271,P=0.000],as well as lower hospital cost [(8.6?1.5) thousand RMB vs (9.5?1.1) thousand RMB,t=-3.421,P=0.000]. Conclusion Nephrostomy shows positive effect on relieving infections after ureteroscopic holmium laser lithotripsy.

Objective To evaluate the clinical value of dual-energy CT in the detection of monosodium urate crystals in patients with gout.Methods One hundred and eight patients who experienced unilateral arthrocele and (or) joint pain in the past two weeks were enrolled into our study.DECT were performed for the upper or lower extremity.Ninety-five patients were enrolled into the gout study group based on the American rheumatism association (ACR) classification standard;The 0.3 linear blended images group were regarded as conventional CT group,DE (80 kV and 140 kV) datasets were reconstructed via gout-recognition software,the pseudo-color images group as the postprocessed group.Imagings were reviewed independently by two senior radiologists.Chi-square test were used for statistical analysis with the SPSS 13.0software.Results In the conventional CT group,DECT scans revealed a total of 298 areas of urate deposition in 51 patients;The sensitivity,specificity,and accuracy were 53.7％,84.6％,and 57.4％.In the postprocessed group,401 areas of green urate deposition were detected in 69 patients,the sensitivity,specificity,and accuracy were 72.6％,100.0％,75.9％ respectively,the differences had statistical significance (x2 =7.329 and 8.333,P ＜ 0.05).Conclusions DECT gout recognition technology can detect smaller amount of monosodium urate in the other parts of the body,with a great potential in early diagnosis and treatment monitoring of patients with gout.

The X-ray computed tomography (CT) appearance of 4 cases with pulmonary cryptococcosis (PC) diagnosed by pathological examination in our hospital was retrospectively analyzed. The appearances of PC on CT were various: solitary lesion in 1 case, multiple lesions in single lobe in 2, and multiple lesions in multiple lobes in 1. There were total 52 lesions in 4 cases; the diameter of nodules or masses was 3 - 75 mm. Cavitations were found in 1 case; lesions appeared obviously enhanced and one lesion showed central necrosis. Two cases underwent pulmonary lobectomy; and 2 cases received core cutting needle biopsies, after antifungal therapy for 3 months to 1 year the lesions showed being absorbed. In summary, the CT appearance of PC is non-specific with various modes and forms. PC should be considered when multiple nodules or masses scattered in subpleural zone, accompanied with ground-glass opacity and obviously enhanced. The examination of pathogen and pathology at the beginning is crucial for improving diagnostic accuracy.

OBJECTIVE To understand the relationship between the detection rate of Pseudomonas aeruginosa in Children′s Hospital wards from Jan 2008 to Sep 2008 and disinfection and isolation in the department and investigate the change in antimierobial resistance of P.aeruginosa to provide basis for reasonable use of antibiotics in clinical practice.METHODS The clinically isolated P.aeruginosa strains were collected,cultured and identified by paper diffusing method.The results were evaluated according to the relevant documents of NCCLS of USA.RESULTS The resistant rates of P.aeruginosa to Ampicillin,Ampicillin/Sulbactam,ceftriaxone,cefazolin and SMI were higher than 98%.Their resistant rate to Levofloxacin and IMP was the lowest(about 2% or so).CONCLUSIONS Effective disinfection and isolation of P.aeruginosa should be performed.Selection of antimicrobial drugs should be according to the results of drug susceptibility,reduce the rate of bacterial resistance.

Objective To study the CT and MRI features of peripheral primitive neuroectodermal tumor(PNET).Methods CT and MRI appearances of 10 patients with PNET pathologically proved were analyzed retrospectively.All cases underwent CT scan and 2 cases underwent MR imaging examination.There were 5 men and 5 women,the age ranged from 11 years to 54 years old(mean 24 years).Results The tumors were located in the chest wall(n=4),abdomen and sinus(n=2,each),cervical vertebra and humerus(n=1,each).The lesions in the chest wall and abdomen appeared as larger masses(5~16 cm in diameter).The masses were circular and lobulated with heterogeneous density and in company with bone destruction or periosteal reaction.The solid masses were enhanced after contrast medium administration.The lesions in the sinus showed the soft tissue mass with bone erosion.The lesions in bone demonstrated local iso-intensity on T1WI,hyper-intensity on FSE T2WI and on FATSAT T2WI.There were distant metastasis in 3 cases.Conclusion The clinical and imaging features of pPNET in chest wall were more specific than in other locations.CT and MRI can showed intra-tumor structure,and are helpful in delineated the extent of tumor,predicting resectability and monitoring treatment.

Objective To assess the correlation between inflammation,specific immune response and coronary heart disease(CHD). Methods Thirty healthy cases passed the health examination were taken as the control group. Eighty cases who were diagnosised into CHD,affirmed by coronary angiography,were divided into three groups: acute myocardial infarction (AMI) group(26 cases),unstable angina pectoris (UP) group (24 cases),and stable angina pectoris (SP) group(30 cases). All the cases were tested on the concentrations of C-reactive protein(CRP),IgA,IgG,IgM in serum. Results The serum indices of CRP,IgG,IgA in AMI group and UP group were significantly difference than those in the control group (P0.05). Conclusion The correlation between inflammation and immune system activation are closely associated with CHD.

Objective To analyze the DWI characteristics of intracranial epidermoid cyst (ECs),and to evaluate “T2 shine-through” effect. Methods There were 15 cases of ECs confirmed by pathology, in which 3 cases had the residual tumor after surgery. The ADC value was measured in ECs,deep white matter and CSF,and the comparison was made between DWI and FLAIR in showing the tumors. Results (1) The lesions in 15 cases were high intensity on DWI, their ADC value was from 0.80?10 -3mm2/s to 1.23?10 -3mm2/s, the average was (1.05?0.14)?10 -3mm2/s. But the ADC value of deep white matter was from 0.74?10 -3mm2/s to 1.09?10 -3mm2/s, the mean was (0.88?0.09)?10 -3mm2/s. The ADC value of CSF was from 2.98?10 -3mm2/s to 3.78?10 -3mm2/s,the mean was (3.31?0.21)?10 -3mm2/s. The ADC value of ECs was significant lower than that of CSF ( P