Cytokines involve in many diseases. It' s reported that reduction or increase of cytokines is an important factor to lead to immunological diseases in nervous system. Other studies have found that certain cytokines in the different stages of the same disease play a different role. Understanding the specific mechanisms of cytokines in autoimmune diseases of the nervous system is necessary for providing theoretical basis of new ways to treat these diseases. This article will review the progress of some main cytokines in nervous systemic immunological diseases.

Objective To introduce the recent studies about the lymph node-targeted chemotherapy for gastric carcinoma. Methods The literatures on the lymph node-targeted chemotherapy for gastric carcinoma in recent years were collected and reviewed. Results The lymph node-targeted chemotherapy for gastric carcinoma was effective because it couled improve the drug concentrations in regional lymph node. Conclusion As a part of multiple treatments for gastric carcinoma, lymph node-targeted chemotherapy will be further developed.

The incidence of carotid stenosis disease increases with age,and it is one of the major causes of ischemic stroke.In recent years,with the rapid development of endovascular treatment technology,the therapeutic means,including carotid angioplasty and stenting,has been widely carried out both at home and abroad.This article reviews the advances in endovascular treatment of carotid stenosis.

Objective To observe the effect and study intervenient action of Kuijieling Granules (KG) in treating ulcerative colitis (UC) during the active period by the clinical experiment. Methods The patients of active UC (damp-heat syndrome) were randomly divided into 2 groups (KG+SASP group and SASP group) to observe pathological changes of the mucosa,syndrome effect and main symptoms scores,and the protein expression of TLR4,CD14 and NF-?B p65 were detected by immunohistochemistry. Results KG+SASP group had better effect than SASP group in syndrome and mucosa (P0.05). There were outstanding differences among the pathologic grade of UC about IA of TLR4,CD14 and NF-?B p65. The expression of TLR4,CD14 and NF-?B p65 were decreased by treatment (P

Objective To establish the animal model of damp-heat syndrome(DHS) and explore the expression of aquaporin(AQP) 3,4 gene in gastric mucosa.Methods Thirty SD rats were randomized into 3 groups and observed for 20 days.Group A(normal group,NG) was fed with routine method.Group B(Pi deficiency syndrome group,PDS) was fed alternatively with routine method and aqua of cassia angustifolia Vahl.Group C(DHS) was fed with fat,honey liquid and treated with damp-heat environment.The expression of AQP 3,4 gene in the 3 groups were determined by fluorescence quantitative polymerase chain reaction(FQ-PCR).Results The symptoms and signs in Group C were simiar to the clinical DHS.Expression of AQP3 gene in DHS was statistically significant higher than that in PDS and NG(P

Objective To investigate the inhibitory effect of cyclooxygenase-2 (COX-2) inhibitor celecoxib alone and in combination with somatostatin (SST) analog octreotide on human gastric cancer. Methods Fifty one gastric cancer patients were randomly divided into 3 groups. The patients in control group(n=15)took no medicine before gastric cancer resection. Celecoxib group(n=18)patients took celecoxib orally 0.2 g/d for 7 days before surgery. Combination group(n=18) patients took celecoxib orally 0.2 g/d and were injected octreotide 100 ?g/d subcutaneously for 7 days before surgery. The resected specimens were examined histologically, including status of tumor necrosis, inflammatory cells infiltration and fibrous tissue proliferation. The microvessel density (MVD) and the expression of COX-2 in gastric cancer were evaluated by immunohistochemical methods. The apoptosis of tumor cells was measured by terminal deoxynucleotidyl transferse-mediated-dUTP nick end labeling. The expression of somatostatin receptor (SSTR) -1,SSTR-2 and SSTR-3 mRNA in gastric cancer tissue obtained by biopsy in all patients were detected by using real time fluorescence quantitative RT-PCR. Results Compared with control group, gastric cancer tissue necrosis increased significantly(P

Objective To compare dual-energy X-ray absorptiometry T-score concordance between hip and lumbar spine, and to estimate the value of T-score of the hip for diagnosing osteoporosis in the elderly. Methods From September 2000 to May 2002, 123 males aged 60-89 years (average 72.0?7.4) and 137 females aged 60-88 years (average 67.3?6.1) were selected in the study based on the exclusion of subjects with apparent abnormalities affecting bone mass or taking medications which might affect bone metabolism. Detailed questionnaire included lifestyle factors such as smoking, drinking, exercise and dietary habits, etc. Radiographs of the thoracic and lumbar spine were taken. Patients' BMD were measured by DEXA (DMS, Challenger) at lumbar spine (L 2-4) and left hip in posterior-anterior projection. The right hip was scanned if patients had a history of fractures or any bone disease in the left hip. All data were statistically analysed using SPSS 8.0 system. Results The average T-scores of bone mineral density of the lumbar spine were higher than that of the hip in all male patients and in 65-, 85- age groups of female patients(P

Objective To construct and identify recombinant expression plasmid of small interfering RNA (siRNA)targeting hepatitis B virus X protein(HBx), and observe its effect on mitoehondrial function in healthy liver cell line steadily expressed HBx gene (HL-7702/HBx). Methods Two siRNA sequences containing short hairpin structure, which target on the total length HBx gene, were synthesized and cloned into the vector psiRNA-Hh1GFPzeo to eonstruct recombinant expression plasmids pX1 and pX2. Non-specific recombinant pScr plasmid served as control. After siRNA transfected into HL-7702/HBx cells line by liposome, reverse transcription-polymerase chain reaction (RT-PCR) and Western blot were performed to identify the suppressive effect on HBx expression. Levels of intraeellular reactive oxygen species (ROS) and mitochondrial membrane potential (△ m) were determined by flow cytometry. The experimental results were compared by analysis of variance. Results Successful constructions of pX1 and pX2 were confirmed by restriction enzyme digestion and sequencing. The expressions of HBx mRNA and protein after 48 h of transfection into HL-7702/HBx cells in control group were 0.65± 0.12 and 0.62± 0.09, respectively, which were both higher than those (0.33±0.10 and 0.19±0.08, respectively) in group pX1 (t=4.73, P<0.05; t=7.53, P<0.05) and those (0.48±0.10 and 0.37±0.11, respectively) in group pX2 (t=2.39, P<0.05;t=4.43,P<0.05). But the inhibition of group pX1 was stronger than that of pX2 (t=2.28,P<0.05). Levels of ROS and △ m after RNA interference were 5.00±0.38 and 33.86±0.50, respectively, while those in control group were 72. 10±0. 55 and 3. 57±0.26, respectively (ROS: t=276.22, P<0.05; △ m: t=107.15, P<0.05). Conclusions siRNA targeting HBx can efficiently and specifically suppress the HBx expression in HL-7702/HBx cells, and decrease the level of ROS and increase the level of △ m, thus relieve cellular oxidative stress.