Objective To discusses the reason and effective prevention and control nursing measures in severe craniocerebral injured patients complicated with secondary pulmonary aspergillosis.Methods The reason of 21 severe craniocerebral injury patients infected by pulmonary aspergillosis were analyzed,and comprehensive nursing care was given to these patients,including strict disinfection and isolation,strengthening management of hospital environment,early close observation of disease,applying effective antifungal drugs in time,strengthening the medication nursing and nutrition support,standard antibiotic application,attention to the respiratory tract management,basic and college nursing.Results 13 cases were recovered,4 cases were better,4 cases died,the mortality rate was 19％.Conclusions Reasonable treatment and nursing care to the severe craniocerebral injury patients complicated with secondary pulmonary aspergillosis can effectively prevent and control the spread of pulmonary aspergillosis in the hospital,improve the cure rate,reduce the complications and the mortality rate,and ensure the safety of patients.

Objective To investigate the effects of different degrees and duration of hypothermia on severe traumatic brain injury in dogs. Methods Thirty-six one-year-old healthy dogs weighing 13-15 kg were used in this study. Traumatic brain injury model was estabhshed according to the method described by Feeney. Orthogonal design was used in this experiment. Two empirical factors: temperature (factor A) and duration (factor B) were used. Body temperature was maintained at 38 ℃ (A1), 31 ℃ (A2) or35 ℃ (A3) for 6 h (B1) or 12 h (B2).The dogs were divided into 6 groups: group A1B1 , A1B2, A2B1, A2B2, A3B1 and A3B2. Blood gas analysis was performed after the target temperature was maintained for the target duration. Neurological deficit was assessed and scored (0 = no deficit, 500 = severe neurological deficit) and blood samples were obtained at 24, 48 and 72 h after brain injury for determination of serum concentrations of neuron-specific enolase (NSE) and myelin basic protein (MBP) and plasma S-100β protein concentration. Brains were removed at the 72 h after brain injury for determination of Bcl-2 and Bax expression and detection of apoptosis in the brain. Results Hypothermia significantly decreased serum NSE, MBP and plasma S-100β concentrations, neuronal apoptosis and NDS scores;up-regulated Bcl-2 expression and down-regulated Bax expression in brain tissue in the 4 hypothermia groups (group A2B1, A2B2, A3B1 and A3B2) as compared with the control groups (group A1 B1, A1 B2). Best neuroprotective effects were observed in group A3 B1 (35 ℃ for 6 h) in terms of serum NSE, NBP and plasma S-100β concentrations, neuronal apoptosis and cerebral Bax and Bcl-2 expression, but there was no significant difference in the NDS scores among the 4 hypothermia groups. Conclusion Hypothermia can provide neuroprotection in a dog model of traumatic brain injury but the neuroprotective effect is independent of the degree and duration of hypothermia.

Objective To observe the efficacy of lowering blood pressures and TCM syndrome on the mild to moderate hypertension via the treatment of auricular syndrome differentiation combined with magnetic point application;To provide reference for clinical treatment of hypertension by non-pharmaceutical therapy. Methods Totally 260 patients with mild to moderate hypertension were randomly divided into control group and treatment group, 130 cases for each. The control group was given Norvasc by conventional oral dosage, and the treatment group was given auricular syndrome differentiation and Yongquan (KI1) magnetic point application additionally. SBP, DBP and TCM syndrome scores of the two groups were observed before and after treatment to evaluate the antihypertensive efficacy and TCM syndrome efficacy. The side effects were also observed. Results After treatment, SBP of the two groups decreased significantly (P<0.05), with no significant difference (P>0.05). DBP of the treatment group decreased significantly (P<0.05), with significant difference compared with the control group (P<0.05). There was no significant difference in antihypertensive efficacy between the two groups (P>0.05). TCM syndrome of treatment group improved significantly compared with the control group (P<0.05). The total effective rate of TCM syndromes in treatment group was better than the control group (P<0.05), without side effects. Conclusion Auricular syndrome differentiation combined with magnetic point application can not only significantly improve the TCM syndrome of patients with mild to moderate hypertension, but also has synergistic hypotensive efficacy and high safety.

objective To evaluate the clinical effect of touqing capsule associated with Fengchi on the vertebral artery type of cervical spondylosis(CSA)in the attack stage.Methods A total of 100 patients with CSA, according to the doctor order, were randomly divided into a control group and a treatment group respectively, with 50 patients in each. The control group was treated with acupuncture therapy, the treatment group was additionally treated with Touqingcapsule on the basis of the control group, and both groups were treated for 20 days. The hemodynamics was detected by transcranial doppler, the change of TCM syndrome integral was observed before and after the treatment, and the clinical efficacy was evaluated.Results After treatment, the treatment group dizziness (1.10 ± 0.33 vs. 2.01 ± 0.26,t=14.909), neck shoulder ache (0.87 ± 0.16 vs. 1.92 ± 0.69,t=10.266), numbness (0.95 ± 0.14)vs. 1.87 ± 0.16,t=29.844), the total integral (2.16 ± 0.75 vs.3.89 ± 1.14,t=8.756) integral were significantly lower than the control group(P<0.01).Treatment group RVA (40.03 ± 5.89 cm/svs. 34.26 ± 6.26 cm/s,t=4.628), the LVA (42.14 ± 5.42 cm/svs.33.89 ± 6.08 cm/s,t=6.985) rise and PI (0.79 ± 0.14 vs.1.04 ± 0.19,t=7.312) reduction were significantly better than those of the control group (P<0.01). The total effective rate in the treatment group was significantly better than the control group (91.7%(44/48)vs. 74.5%(35/47),χ2=3.863, P=3.863).Conclusion Touqing Capsule associated with Fengchi is effective for CSA. Its functional mechanism is correlated with improvement of haemodynamics of vertebral-basilar artery of the CSA patients.

SuperparamagneticSiO2@Fe3O4microspherewithcore-shellstructurewaspreparedbythe method of hydro-thermal and St?ber method, and modified by (3-aminopropyl) triethoxysilane ( APTES) . The obtained amino-Fe3 O4@SiO2 microsphere with controlled surface charge properties was characterized by SEM and TEM, and the surface electric property of amino-microsphere was investigated by Zeta potential measurement. The microsphere was used to extract DNA from human blood, and the solid phase extraction method by microspheres was developed. The mechanism of action between Aminated-Fe3 O4@SiO2 microsphere and DNA was explored, and the gel electrophoresis and PCR test were done on the extraction product. The result showed that genomic DNA with high purity was successfully extracted from whole blood by using amino-Fe3 O4@SiO2 microsphere, with the extraction rate about 70%, the saturated adsorption capacity for DNA was about 40 ng/μg, and the elution could be directly used for further bio-analysis.

To investigate the effects of medicated serum prepared with Chinese herbal medicine Zhizhen Recipe (ZZR) on activity of nuclear factor-κB (NF-κB) and expression and function of P-glycoprotein (P-gp) in human colorectal cancer multidrug-resistant cell line HCT-8/VCR.

An online enrichment-separation system of C18 and inductively coupled plasma-mass spectrometry (ICP/MS) with flow injection （C18-FI-ICP/MS） was established for the determination of trace heavy metals in seawater. The optimized experimental parameters including pH，C18 column，concentration of methol in elute and the velocity of elute were investigated. This system was applied to the online preconcentration , separation and determination of trace heave metals in seawater samples successfully. The detection limit　(3σ) for Cd, Pb, Co, Ni and Zn was 0.03, 0.09, 0.1, 0.1, 0.3 μg/L respectively. The RSD of Cd, Pb, Co, Ni, and Zn for six measurements of seawater was 6.8%，3.4%，1.3%，2.6%，0.5%，respectively. The accuracy was verified with spiked recovery test. The recovery for five elements was 91.3%，95.1%，100.4%，100.3%，95.2%, respectively.

Objective To investigate the effect of moderate static magnetic fields (SMF) on secretion of inflammato?ry factors tumor necrosis factor-α(TNF-α), interleukin-6 (IL-6) and interleukin-8 (IL-8) in human monocytic leukemic cell line THP-1. Methods THP-1 cells at logarithmic phase were divided into control group and magnetic treatment group. CCK-8 method was used to detect cell proliferation after THP-1 cells were exposed to 60 mT, 200 mT and 400 mT static magnetic fields at 18, 24 and 48 h. Then THP-1 cells were divided into control group, magnetic treatment group, LPS activation group and LPS+SMF treatment group. When magnetic treatment group and LPS+SMF treatment group were ex?posed to SMF at 18, 24 and 48 h, the levels of the cytokines TNF-α, IL-6 and IL-8 were determined by ELISA. Results (1) 60 mT, 200 mT and 400 mT SMF had no significant effects on cell proliferation in THP-1 cells (P>0.05). (2)THP-1 cells secreted more TNF-αand IL-6 in 24 h than 18 h in every group, while IL-8 didn′t change. Compared with 24 h, the secre?tion of TNF-αdecreased and IL-6 didn′t change, while IL-8 increased in 48 h. At three sampled time THP-1 cells of LPS activation group secreted more TNF-α, IL-6, IL-8 than those of control group and magnetic treatment group. After magnetic treatment THP-1 cells of LPS+SMF treatment group secreted less TNF-α, IL-6, IL-8 than those of LPS activation group (P<0.05). Conclusion Static magnetic field may have some inhibitory effects on release of TNF-α, IL-6, IL-8 from THP-1 cells, which can provide basic data for the treatment of rheumatoid arthritis.

Objective To establish a method of fingerprint analysis on Huai Chrysanthemum mori-folium in Henan Province by GC.Methods The GC method was applied with a fiber column(30 m ? 320 ?m,0.25 ?m)coated with HP-5(5%)-diphenypolysiloxane.The detector was FID detector;The temperature of the detector was 180 ℃;Nitrogen was used as the carrier gas with its flow rate 2.3 mL/min;The flow rate of atmosphere was 300 mL/min,and the flow rate of hydrogen was 30 mL/min in temperature programmer.The fingerprint of Huai C.morifolium in Henan Province was established.Results Twenty-seven common peaks were found in GC fingerprint of Huai C.morifolium in Henan Province and good similarities with correlation coefficients over 0.9 were found in the fingerprints compared between the fingerprint of Huai C.morifolium in Henan Province from different sources and standard fingerprint.Conclusion The method is accuate,simple,and can be used for the quality control of Huai C.morifoliumin Henan Province.

Objective To understand the distribution and antimicrobial resistance of pathogens isolated from blood culture in a hospital in Sanya city.Methods Blood culture specimens and antimicrobial susceptibility testing results in this hospital from January 2013 to December 2015 were analyzed retrospectively.Results A total of 356 isolates of pathogenic bacteria were isolated from 3 195 blood culture specimens,the positive rate was 11.14％,including 215(60.39％)gram-negative bacterial strains,122(34.27％) gram positive bacterial strains,and 19(5.34％) fungi strains.The top 3 gram negative bacteria were Escherichia coli (n =90,25.28％),Klebsiella pneumoniae (n =60,16.85％),and Burkholderia pseudomallei (n =24,6.74％);the most common gram positive bacteria were Staphylococcus aureus (n =42,11.80 ％),coagulase-negative staphylococcus (n =38,10.67 ％),and Streptococcus spp.(n =33,9.27 ％).Resistance rates of Escherichia coli and Klebsiella pneumoniae to cefoperazone/sulbactam,amikacin,and carbapenems were all lower than 10.00％;resistance rates of Burkholderia pseudomallei to most antimicrobial agents were lower than 10.00％.There were no strains of main gram-positive bacteria that were found to be resistant to linezolid and vancomycin.Conclusion Gram-negative bacteria are the main pathogens causing bloodstream infection in this hospital in recent years,especially the isolation rate of Burkholderia pseudomallei is higher,which should arouse more attention in clinic.