SD male rats aged 21 days were continuously exposed to light for 30 days in order to inhibit secretion of melatonin (MT). Changes in serum MT, testosterone, corticosterone, thyroid hormones (T_ 3 , T_ 4 ), adrenalin and noradrenalin were determined. The results showed that MT inhibited the functions of gonad, adrenal and sympathetic nerve, but increased the function of thyroid in pre-puberty rats.

0.05), but the index of Hf was significantly lower in heart blood stasis syndrome in CHD group than in healthy control group (P

Objective To research photoelectric facial blood flow volume characteristic of premature coronary heart disease of heart blood stasis syndrome (HBSS) and phlegm syndrome (PS), and its relation with NO and ET. Methods Patients of premature coronary heart disease of HBSS (36 cases) and PS (35 cases) were selected, with 31 health people as control. The parameter of photoelectric facial blood flow volume was detected with GD-3 photoelectric blood stream plethysm (PBSP) of the facial color-diagnosis. NO was determined by nitrate reductase method and ET was determined by RIA. Results Hb, He, Hf, Hb/Tab and Hf/Hb of HBSS decreased obviously compared with the control (P

Objective To explore the mechanism of six-segment classification of femur intertrochanteric fracture in a three-dimensional finite element model of upper thigh-bone and its clinical relevance.Methods The left upper thigh-bone of a normal male volunteer of 60 years old was scanned using 64-slice CT.After the images were stored in the format of JPG,they were input into photoshop 7.0 for three-dimensional creation.The three-dimensional images were used to make a three-dimensional finite element model of upper thigh-bone using software Super 93.The model consisted of 764 nodes and 531 units (including 306 compact bone units and 225 cancellous bone units).In this model,the stress distribution at the trochanter during human tumbling was analyzed by imitating adduction,abduction,adduction-intemal rotation and abduction-external rotation of the hip,as well as intense muscular contraction of gluteus medius,piriformis and iliopsoas during adduction-internal rotation of the hip.Results Analysis of stress nephogram showed that the stress was distributed mainly at the exterior cortical bone and spread to the inter-trochanteric part in simple hip adduction (two-part fracture) and distributed mainly at the interior cortical bone and spread to the inter-trochanteric part in simple hip abduction (two-part fracture).In adduction-internal rotation or abduction-external rotation of the hip,the stress was focused on the anterior and posterior walls of the greater trochanter and trochanteric part and spread to the exterior and interior cortical bone and the inter-trochanteric part (three-or four-part fracture).During intense muscular contraction of gluteus medius,piriformis and iliopsoas during adduction-internal rotation of the hip,the stress was focused on the anterior and posterior walls of the greater trochanter,lesser trochanter and trochanteric part and spread to the exterior and interior cortical bone and the inter-trochanteric part (five-or six-part fracture).Conclusions The six-segment classification of femur intertrochanteric fracture explains complex stress distributions after human falling.When combined with three-dimensional CT reconstruction,it is more intuitive than other classifications so that it can provide more definite surgical advice for different types of fracture.

Objective To explore the effect of comprehensive rehabilitation on patients with knee ankylosis and contracture of popliteal fossa after severe burn. Methods 35 patients with severe burn on lower limb (containing the around skin of popliteal fossa) accepted exercise therapy, hydrotherapy, occupation therapy (including pressure therapy), electrotherapy, etc. They were assessed with Functional Independence Measure (FIM), range of motion and muscle strength of knee before and after treatment. Results The scores of FIM, range of motion and muscle strength of knee improved after treatment (P<0.01). Conclusion Comprehensive rehabilitation can improve activities of daily living and function of knee after severe burn.

【Objective】To observe the morphologic changes of organs in mice with spleen deficiency and the effect of Qiangji Jianli Oral Liquid(QJOL).【Methods】Forty healthy male NIH mice were randomized into 4 groups:A(normal),B(model),C(treated with QJOL)and D(treated with Si Junzi Decoction).Except group A,the mice in other 3 groups received intraperitoneal injection of reserpine to induce spleen deficiency.Meanwhile,groups C and D received QJOL and Si Junzi Decoction in the dosage of 26?g?kg~(-1)?d~(-1) by gastric gavage respectively,and groups A and B received the same volume of distilled water for 21 days.Twelve hours after last administration,the body weight and pathological changes of liver,kidney,spleen and thymus were examined.【Results】In group A,the body weight decreased,and liver,kidney,spleen and thymus gland showed different degrees of damage.QJOL and Si Junzi Decoction could increase the body weight and promote the repair of the organs.【Conclusion】Different degrees of damage can be found in liver,kidney,spleen and thymus gland of mice with spleen deficiency,and spleen-strengthening medicinal herbs can promote their repair,indicating that the theory of spleen controlling the muscles,being the solitude organ and localized the center to cultivate the surroundings organs like the earth,which is described in Huangdi's Canon of Medicine,has the morphologic evidence.

【Objective】To observe the effect of Buzhong Yiqi Wan(BYW)on thyroid hormones in rat model of spleen-asthenia.【Methods】Thirty-four SD male rats were randomized into 3 groups:normal control group(N=10),spleen-asthenia model group(N=12),BYW group(N=12).Except the normal control group,the rats in other groups were given the decoction of Radix et Rhizoma Rhei(4 g?d-1 for each rat)to induce spleen asthenia,and meanwhile,BYW group reveived BYW(3.9 g?kg-1?d-1).The treatment lasted 20 days.Radioimmunoassay was used to detect serum levels of triiodothyronine(T3),thyroxine(T4),reverse triiodothyronine(rT3)and thyroid stimulating hormone(TSH).Meanwhile,the body weight,spleen and thymus weight as well as their ratio with the body weight were also observed.【Results】Serum T3 and T4 levels,spleen and thymus weight as well as their ratio with the body weight were decreased in the model group as compared with those in the normal control group(P

Objective To investigate the effects of Huangqi Sijun Decoction on the levels of thyroxin and cAMP and cGMP in model rats with spleen deficiency syndrome and to explore its therapeutic mechanism.Methods Male SD rats aged three months were randomly divided into 3 groups:normal control group,spleen deficiency model group and Huangqi Sijun Decoction group.The rat models of spleen deficiency syndrome were established by gavage with the decoction of Rhubarb.The serum levels of thyroxin(T4),3,5,3'-triiodothyronine(T3),thyrotropin(TSH)and plasma levels of cAMP,cGMP were determined.Results T3 and T4 were significantly lower but the levels of cAMP and cAMP/cGMP were significantly higher in the spleen deficiency models than those in the normal control group(P

[Objective] To explore the relationship between neuroendocrine changes caused by melatonin (MT) hypo-secretion and kidney-in deficiency syvndrome during prepubescence. [Methods] Thirty SD rats after ablactation were randomly divided into normalgroup (group. A), model group (group B) and herbal medicine group (group C, treated with kidney-yin-nourishing ande(?)ficiency-fire-clearing herbs). Rats in groups B and C were exposed to light continuously for 30 days and simultaneously groupC was given kidney-yin-nourishing and deficiency-fire-clearing herbal medicine (13.3 g?kg-1?d-1, qd) for 30 days . After treatment, blood samples were collected at 0:00-2:00 o'clock to observe the changes of blood MT, testosterone (T), epinephrine (E) and norepinephrine (NE) levels. [Results] Body weight and blood MT level decreased, and T, NE and E levels increased in group A (P

Objective The changes of thyroid hormone and cyclic nucleotides levels in rats with spleen-kidney deficiency syndrome were observed to evaluate the preventive and therapeutic effect of Yougui Wan(YW) for spleen-kidney deficiency syndrome,and to explore the relationship between the spleen and the kidney at microscopic level.Methods Male SD rats were randomly divided into normal control group,model group,and YW group.Rat models of spleen-kidney deficiency syndrome were established by gavage firstly with rhubarb(4g for each rat) decoction and then with hydrocortisone(25 mg?kg-1?d-1,im).The model group and YW group were treated with distilled water and YW(5.8 g?kg-1?d-1) respectively.The general status of rats was observed,and the plasma cyclic adenosine monophosphate(cAMP) and cyclic guanosine monophosphate(cGMP) levels as well as serum thyroid hormones(T3,T4 and rT3) and thyroid-stimulating hormone(TSH) contents were determined in all rats by radioimmunoassay(RIA).Results Compared with the normal control group,the serum contents of T3,T4,rT3 were decreased significantly(P