AIM To study the protective and mechanism of Tfa (total flavone of the flowers of Abelmoschl Manihot L. Medic). METHODS The rats were injected Iso and then the T wave movement value observed. The rats anterior descenging bracnch of left artery were occluded for 48 hours the results of CPK, LDH and disscoate fatty acid of serum and infarct area of myocardium observed. RESULTS Tfa 100,200 mg?kg -1 ,ip depressed the T wave rise by injection of Iso in rats. Tfa 100 mg?kg -1 ?d -1 ?3,ip inhibited the serum CPK and LDH disscoate fatty acid in myocardium induced by coronary artery occluded in rats;also markedly depressed the infarct area in myocardial infarct rats. Tfa 0 03 mg?kg -1 ?d -1 ?3,ip obviously reduced the MDA contents,increased SOD and GSHPx activities in the mitochondria in mice. CONCLUSION Tfa shows a protective effect against myocardial injury. The mechanism may be via against oxygen free radicals and lipid peroxidation,also stabilize the mitochondria function.

Objective:This study was aimed to investigate the value of osteoclast differentiation factor (ODF) and osteoclastogen-esis inhibitory factor (OCIF) detection for clinical diagnosis and assessment of patient condition in bone metastasis of lung cancer. Methods:Data from 186 lung cancer patients who were preliminary diagnosed between July 2009 and April 2012 were analyzed. Cas-es were divided into the bone metastasis group with 82 cases (group A) and the non-bone metastasis group with 104 cases (group B). Concentrations of serum ODF and OCIF in each group were detected by ELISA. Results: ODF and OCIF values of group A were (32.22±6.22) ng/L and (41.23±8.13) ng/L, respectively, which were significantly higher than the corresponding values in group B [(8.35 ±5.42) ng/L and (10.15±4.42) ng/L]. The differences between the two groups were statistically significant (P<0.01). Areas under the re-ceiver operating characteristic curves of ODF and OCIF, which are used to diagnose bone metastasis in lung cancer, were 0.91 and 0.87, respectively, manifesting good diagnostic value. The sensitivity and specificity of ODF in diagnosing lung cancer with bone metastasis were 90.38%and 86.59%, respectively, and those of OCIF were 86.54%and 84.15%, respectively. ODF increased, whereas OCIF de-creased significantly, with increasing bone metastasis. ODF and OCIF concentrations in group A and the group with newly-found bone metastasis were significantly higher than those in group B, with statistically significant differences among these groups (P<0.01). Com-pared with group A, less difference was found in the ODF and OCIF of newly-found bone metastases, without statistical significance be-tween these groups (P>0.05). Conclusion:The serum ODF and OCIF concentrations significantly increase when bone metastasis oc-curs in lung cancer patients. Hence, these variables are useful as indices for monitoring bone metastases and evaluating patient condi-tion. An extensive application prospect is proposed.

BACKGROUND: Total flavone of ginkgo biloba(TFG) can affect on free radical, but the effect on apoptosis induced by cerebral ischemia is unclear.OBJECTIVE: To study the inhibitory effect of TFG on apoptosis induced by cerebral ischemia.DESIGN: Completely randomized controlled experimental study based on experimental animals.SETTING: Department of pharmacology in a university.MATERIALS: Totally 24 SD rats in half genders with clean grade and body mass of(250 ± 50) g, were divided into 4 groups at random: sham-operation group, model group, TFG 40 rmg/kg group and TFG 80 mg/kg group (Certificate No. 01).METHODS: This study was completed in the Department of Pharmacology,Anhui Medical University during October 2001 and January 2002. Incomplete cerebral ischemia was made by ligating bilateral common carotid arteries(CCA) in rats. The cerebral injury was evaluated by brain edema. The apoptosis was determined by terminal deoxy-nucleotidyl transforase-mediated dUTP-digoxigenin nick end-labeling(TUNEL) and transmission electron microscopy (TEM) method. The DNA fragmentation analysis was measured with the diphenylamine reagent method.MAIN OUTCOME MEASURES: Major factor: Effect of TFG on ultrastructral alteration of apoptotic cerebral cortex cells; Secondary factor: Effect of TFG on DNA fragmentation induced by cerebral ischemia.RESULTS: Ligating of bilateral CCA markedly induced apoptotic cell in cerebral cortex. TFG 80 mg/kg significantly inhibited brain edema( P ＜ 0.05 )and decreased the numbers of apoptotic cells in cortex( P ＜ 0.01 ) and improved ultrastructral alteration of apoptotic cells; TFG 40, 80 rmg/kg also inhibited the increase of DNA fragmentation induced by cerebral ischemia (P ＜0.05, P ＜0.01).CONCLUSION: TFG has inhibitory effect on ischemia-induced apoptosis of cerebral cortex and improve the ultrastructual changes of apoptosis. Moreover,TFG can relieve the occurrence of edema of ischemic brain tissue and inhibit the increase of DNA section induced by cerebral ischemia.

Objective To assess hearing and efferent system functions of autistic children.Methods Tests were performed on 30 Autistic children and 15 normal children to evaluate hearing objectively by using otoacoustic emission (OAE) and auditory brainstem response (ABR).The efferent system functions were analyzed through contralateral suppression in OAE.Results The mean ABR Ⅰ-Ⅴ interpeak latencies (IPLs) in children with Autism were significantly reduced than that in the control group.The amplitudes of OAE at 1 kHz and 2 kHz in autistic children were significantly different in two groups.There were no significant differences in contralateral suppressions between the Autistic children and the control.Conclusion Hearing impairment may be more common in children with Autism than in normal children,while for a few Autistic children,their efferent system functions are affected.

Objective To observe the protective effect of total flavone of Camellia (TFC)against cerebral ischemic injury.Methods Decapitation method and close hypoxia method were used to observe the effect of TFC on anoxic tolerance of mice and step down test was used to observe the effect of TFC on learning and memory; after ischemia, the content of malondialdehyde (MDA)and nitric oxide (NO)and the activity of lactate dehydrogenase (LDH)were detected. Rat models with acute incompletely cerebral ischemia were established by means of ligating right common carotid arteries and effect of TFC on cerebral water volume, permeability of cerebral vessels and cerebral histopathological changes were also observed.Results TFC prolonged the grasping time after decapitation and the survival time after anoxia in mice, and improve the learning and memory during the step down test. TFC decreased MDA and NO contents, counteracted the de creases of LDH activities in the mice cerebral cortex, reduced the water volume and permeability of cerebral vessels in ischemic rats and improve the cerebral hitstopathological changes. Conclusion TFC has protective effects against cerebral ischemic injury and the mechanism may be related to the inhibition of free radicals and NO production.

Aim To observe the protective effect and possible mechanisms of Total Flavone of Abelmoschus Manihot L medic(TFA) on myocardial reperfusion injury in rabbits,when it was given as a preconditioning inducer.Method Different doses of TFA were perfused intravenously lasting for 5 min,followed by a 10 min break before 30 min ischemia and 60 min reperfusion in rabbits.Using this preconditioning method,we observed the histo-pathological changes of myocardium,the content of MDA,the activities of SOD and GSH-PX in plasma,and also,the expression of ICAM-1 in myocardium.Results TFA(16,8 and 4 mg?kg~(-1)) significantly ameliorated myocardium ischemical reperfusion injury.The activities of SOD and GSH-PX in plasma were enhanced,while the MDA level was decreased.Meanwhile,the expression of ICAM-1 mRNA in myocardium was down-regulated.Conclusion Pharmacological preconditioning of TFA could protects myocardial ischemic reperfusion injury in rabbits by inhibiting lipid peroxidation and myocardium inflammatory reaction.

Aim To study protective effects of Total of flacone C(TFC) against cerebral ischemia-reperfusion injury.Methods Four-vessel occlusion method was used to make acute cerebral ischemia-reperfusion model. Rats were initiated by ischemia for 30 min followed by 40 min of reperfusion.The electroencephalography(EEG) during cerebral ischemia and reperfusion was recorded.The level of intracellular calcium ion concentration([Ca~(2+)]i) in cerebral cells after ischemia was measured by using a Ca~(2+) indicator Fura-2/AM.Superoxide dismutase(SOD),Glutathione peroxidase(GSH-Px),Lactate dehydrogenase(LDH),nitric oxide Synthase(NOS) activeties and Malondialdehyde(MDA),Nitric Oxide(NO)contents in the ischemia cerebral cortex were measured.Results TFC can improved the EEG change,significantly attenuated the decrease of the intracellular calcium ion concentration([Ca~(2+)]_i), remarkly increased GSH-Px,SOD and NOS activities in the cerebrum,inhibit the decrease of LDH activity and NO,MDA contents.Conclusion TFC has protective effects on cerebral ischemia-reperfusion injury,the mechanism may be related to attenuating free radical,[Ca~(2+)]i overload and NO.

OBJECTIVE:To observe the protective effect of total flavone of Abelmoschl Manihot L medic(TFA)on acute myocardial ischemic and anoxic injury in mice.METHODS:Acute myocardial ischemic model induced by subcutaneous injection of Isoproterenol was adopted to observe the effects of TFA on the abnormal ST segment in lead II and T wave on ECG,my?ocardial water content(MWC)and myocardial index(MI);Moreover the effect of TFA on myocardial anoxic tolerance was ob?served in trachea clamping mice.RESULTS:TFA could remarkably ameliorate the abnormal changes of ECG and significantly inhibit the increase of MWC and MI of model group.It was found that the ECG lasting time was much longer in the groups pretreated with TFA.CONCLUSION:TFA plays a protective role in myocardial ischemia and anoxic injury in mice.

AIM To observe the protective effect of total flavone of Abelmoschl Manihot L medic(TFA) on myocardial ischemia reperfusion injury(IRI) in the rat heart in vitro. METHODS We adopted the method of in vivo perfusion set up by Langendorff to observe the changes of some markers, such as MDA,SOD,CPK,LDH,etc in the myocardial homogenate in the rat heart following ischemia and reperfusion. RESULTS TFA( 100,50,25 mg?L -1) can significantly reduce the lipid peroxidation product malondialdehyde(MDA), increase the activity of myocardium superoxide dismutase(SOD), decrease the transudation of creatine phosphokinase(CPK) and lactate dehydrogenase (LDH) from cardiomyocyte. And also, TFA caused an improvement of nitric oxide (NO) and its synthase, NOS. CONCLUSION TFA play a protective role on myocardial reperfusion injury in the rat in vitro via inhibition of lipid peroxidation, enhancement of anti-oxidation and relaxing of coronary artery.