Objective To study the optimal extraction process of volatile oil in Radix Angelicae Pubescentis.Methods L9(34) orthogonal design was carried out for the optimization of water extraction of volatile oil in Radix Angelicae Pubescentis,and the content of volatile oil was used as referece index.Results The optimal extraction process was as follows:medicinal materials were crashed to go through mesh 80～100,then were soaked in 12-fold volume of water for 10 hours,and were extracted for 11 hours.Conclusion The optimal extraction techndogy is simple and feasible with the extraction rate of volatile oil being 2.66 %.

Objective To evaluate the clinical effect of the use of pathfile and protaper nickel-titanium instruments for machine in root canal preparation.Methods A total of 200 patients suffering from pulpitis and periapical that needs root canal treatment was randomly divided into two groups according to treatment order (100 patients/each group).The experimental group was used pathfile and protaper in root canal preparation,and filled with warm gutta vertical condensation.The control group was used protaper root canal preparatory standards method,and filled with warm gutta vertical condensation.The required time of two sets of root canal preparation and the number of equipment broken were recorded,and X-ray film at the preoperation intraoperation and postoperation were used to evaluate effect of preparation and root filling.Results In the root canals prepared of the two groups,no issued offset,no side wear,no step-forming,and no position changed of the apical foramen were occurred,which was consistent with preperative X-ray film.One case was occurred fracture of instrument in the experimental group and the control group,it was Slfile.Tooth position was right mandibular,second molar nearly the root apical 1/3,and upper left second molar mesial buccal root apical position.The root canal preparation time of experimental group was significantly shorter than the control group[the front teeth:(4.73 ± 1.12) min vs (6.32 ± 1.84) min,t =2.243,P ＜0.05; the premolars:(7.83±1.23)min vs (10.25±2.68)min,t =2.475,P ＜0.05; the molars (10.14 ± 1.18) min vs (15.43 ± 5.94) min,t =2.991,P ＜ 0.01].Postoperative X-ray showed that 2 cases were occurred small amount of paste overfilled,the root canal closed tight,no less filling; and 3 cases of the control group occurred paste overfilled,no less filling too.Conclusions The use of nickel-titanium instruments pathfile and protaper for machine in root canal preparation is feasible,and improves the efficiency of root canal preparation.

Objective: To prepare piroxicam nanostructured lipid carrier and investigate its transdermal absorption behavior in vitro. Methods:Piroxicam nanostructured lipid carrier was prepared by a melt-emulsion ultrasonication and low temperature-solidifica-tion method. The physicochemical properties such as appearance, morphology, particle size distribution, PdI and zeta potential of pi-roxicam nanostructured lipid carrier were evaluated. The transdermal absorption in vitro was investigated using Franz diffusion cells. Results:Piroxicam nanostructured lipid carrier was clear and transparent with small spherical shape as seen under a transmission elec-tron microscope. The particle size distribution, PdI and zeta potential was (106. 4 ± 31. 6) nm, (0. 217 ± 0. 07) and ( -31. 6 ± 2. 5) mV, respectively. Piroxicam nanostructured lipid carrier had higher cumulative transdermal amount in 12 h than piroxicam solution. Conclusion:The nanostructured lipid carrier can remarkably improve piroxicam permeation into skin, which provides reference for the new dosage form for the topical use of piroxicam.

Lung adenocarcinoma is usually thought to follow a linear multistep progression schema,that is precancerous lesions progress to adenocarcinoma in situ,and progress to micro-invasive adenocarcinoma,eventually some lung adenocarcinoma happen diffusion.However,recent findings reveal that this linear progression schema might not occur in all lung adenocarcinoma subtypes,and some subtypes show a kind of non-linear progression schema.The study of these two kinds of schemas can help for early detection of lung adenocarcinoma.

Objective To investigate the possibility of pulp regeneration of different irreversible pulpitis with absorbable gelatin sponge as the scaffold,and dental seed cells,induced molecules and MTA closed.Methods 150 patients with irreducible pulpitis,who were removed the crown infection pulp and retained the root living pulp,according to the visiting sequence,were randomly divided into two groups.The experimental group was given absorbable gelatin sponge as the scaffold and MTA closed.The control group was given calcium hydroxide coverage.The symptoms situation and pulp situation were observed.Results 6 months after surgery,the success rate of acute pulpitis and chronic closed pulpitis in the experimental group was 76.5％ and 47.0％,which were significantly higher than those of the control group (14.3 ％,8.8％) (P ＜ 0.01).In the experimental group,the success rate of chronic hyperplastic pulpitis and chronic ulcerative pulpitis was 33.0％ and 35.0％,which were higher than those of the control group(9.1％,6.0％),but the differences between the two groups were not statistically significant (P ＞ 0.05).Conclusion Pulp regeneration in vivo is possible with absorbable gelatin sponge as the scaffold and MTA,pulp regeneration of acute pulpitis and chronic closed pulpitis are more likely to induce.

[Objective]Discussion on academic ideas and clinical experience of Professor XIE Jingri in treatment of ulcerative colitis(active phase). [Method] From the disease affiliation, etiology and pathogenesis,syndrome differentiation, clinical adding and subtracting, disease aftercare to expound the academic viewpoints and clinical experience of Prpfessor XIE Jingri in treating ulcerative colitis(active phase), summarize his prescription regularity and treatment characteristics,and put an example to exemplify that.[Results]Professor XIE Jingri thinks that spleen deficiency is its fundamental pathogenesis,dampness-heat is the key factor of attack, the liver is closely related to the lung, spleen and large intestine,it could adjust the emotions and circulation of Qi, blood and body fluid.Therefore,we shuold take the method of strengthening spleen and nourishing Qi and dampness-heat clearing simultaneously as basic therapy, and pay more attention to the treatment lying in regulating the liver, use the method of regulating qi activity and activating circulation to remove blood stasis, focus on disease aftercare at the same time,the clinical therapeutic effect is satisfied.[Conclusion]The clinical experience of Professor XIE Jingri in treatment of ulcerative colitis(active phase) could effectively alleviate symptoms and reduce relapse rate,it is worth to be summarized and popularized.

Aim To investigate the inhibition of MEK/ERK pathway affecting the sensitivity of human breast carcinoma cells SK-BR-3 to endoplasmic reticulum(ER) stress-induced apoptosis and wish to find new targets for human breast carcinoma chemotherapy.Methods Different concentrations(0,1.5,3,6,9 and 12 ?mol?L-1) tunicamycin(TM) treated human breast carcinoma cells SK-BR-3 for 48 h,then propidium iodide(PI) staining measured apoptotic cells in Flow Cytometry(FCM).Different times(0,6,12,24 and 36 h) of TM(3 ?mol?L-1) treated SK-BR-3 cells,Western blot measured proteins GRP78,ERK1/2 and pERK expression.MEK inhibitor U0126(20 ?mol?L-1) pretreated cells for 1 h before treatment with TM(3 ?mol?L-1) in different concentrations and times,measured above identical indexes and compared with their diversities of treatment with U0126 or not.Results TM induced apoptotic cells

Background:MicroRNAs ( miRNAs ) is a class of small non-coding RNAs that regulate gene expression at posttranscriptional level. They are considered to play a critical role in the tumorigenesis and development of various tumors. Aims:To identify the recurrence-specific miRNAs in peripheral blood of patients with recurrent colorectal adenoma by miRNA array for providing sensitive and specific molecular markers for recurrence prediction. Methods:Fifty colorectal adenoma patients who had undergone polypectomy by colonoscopy were re-examined from Aug. 2012 to Sep. 2013. Four recurrent and four non-recurrent patients were selected and miRNA array was used to detect the expression profiles of miRNAs in peripheral blood samples. Differentially expressed miRNAs were identified between recurrent and non-recurrent patients. Real-time PCR was applied to verify the differentially expressed miRNAs identified by microarray. Results:Seven up-regulatedmiRNAs(hp hsa-miR-548ai,hsa-miR-4446-3p,hsa-miR-139-3p,hsa-miR-27a-5p,hsa-miR-10a,hsa-miR-23a and hsa-miR-486-3p)and 4 down-regulated miRNAs( hsa-miR-124,hsa-miR-3613-5p,hsa-miR-495 and hsa-miR-485-5p)were identified by microarray between recurrent and non-recurrent patients. Among them,seven miRNAs were validated by real-time PCR as consistent with microarray. Conclusions:Some differentially expressed miRNAs may be involved in the recurrence of colorectal adenoma,which gives clues for further investigation on recurrence predictors.

OBJECTIVE To study the molecular cytogenetic alterations of transitional cell carcinoma of the urinary bladder with exfoliated cells by fluorescence in situ hybridization(FISH) analysis of chromosome-specific probes.METHODS FISH was performed using 3,7,9 and 17 of chromosome-specific probes to examine chromosome aberration of exfoliated cells in 50 urine samples from patients with transitional cell urinary bladder carcinoma.RESULTS The frequency of numerical aberration of chromosomes 3,7,9 and 17 was 28%,32%,56% and 38% in urinary exfoliated cells,respectively.Loss of chromosome 9 was the most common finding,but it was not correlated with pathological grade of cancer and stage of the disease.Abnormality of chromosomes 3,7 and 17 was associated with the clinical stage.CONCLUSIONS A number of chromosome aberrations are detected in transitional cell carcinoma of the urinary bladder by FISH technique which provides a basis for further understanding of its molecular pathogenesis.It is a rapid,accurate and very sensitive method and can be used in clinical diagnosis.

Recent meta-analysis based on both clinical trials and epidemiological studies has revealed the relationship of dietary cholesterol,blood cholesterol level and cholesterol lowering drugs (statins) with colorectal cancer.This review summarises the advances in current evidences of linking cholesterol metabolism and risk of colorectal cancer and the underlying molecular mechanisms based on pharmacogenetics and molecular pathology.