Objective To analyze the relationship between anti Mullerian hormone (AMH) levels and abnormal endocrine metab-olism in patients with polycystic ovary syndrome (PCOS) .Methods From January 2014 to December 2015 ,a total of 200 PCOS pa-tients were recruited into experimental group ,180 infertility patients causing by tubal disorder were selected into the control group . According to the level of AMH ,PCOS patients were divided into high AHM group(AMH=10 ng/mL ,98 cases) and low AMH group (AMH<10 ng/mL ,102 cases) .According to the index of insulin resistance(HOMA) ,PCOS patients were divided into high HOMA group(HOMA index=2 .97 ,108 cases) ,and low HOMA group (HOMA index <2 .97 ,92 cases) .Follicle stimulating hor-mone(FSH) ,serum AMH ,estradiol ,luteinizing hormone(LH) ,fasting blood glucose and fasting insulin ,testosterone(T) ,high den-sity lipoprotein(HDL) ,low density lipoprotein(LDL) were detected ,the level of insulin resistance(factor HOMA-IR were calculat-ed ,then internal secretion metabolic differences and the effect of AMH were analyzed .Results The AMH ,HOMA-IR ,LH ,T in the control group were significantly lower than those in the experimental group(P<0 .05) ,body mass index(BMI) in the control group was significantly higher than that in the experimental group(P<0 .05) .BMI ,HOMA-IR in the high AMH group were sig-nificantly lower than those in the low AMH group(P<0 .05) .There were no significant differences on LH and T between the high AMH group and low AMH group (P>0 .05) .AMH ,INS ,BMI in the high AMH group were higher than those in the low AMH group(P<0 .05) .Conclusion AMH in patients with PCOS increased significantly ,and AMH was negatively correlated with BMI and HOMA-IR ,and positively correlated with testosterone .

OBJECTIVE:To improve the quality standard of Jianpi zhixiening granules. METHODS:TLC was applied for qual-itative identification of Scutellaria baicalensis,Crategi Fructus,Lonicerae japonicae,Codonopsis Radix,Nelumbo nucifera,Copti-dis Rhizoma. The contents of berberine hydrochloride and baicalin were determined by HPLC. The determination was performed on Shimadzu VP-ODS column with mobile phase consisted of methanol-acetonitrile-water (46:30:42,V/V/V,berberine hydrochlo-ride))(0.1% sodium dodecylsulphate,0.1% phosphoric acid,methanol-0.4% phosphoric acid(50:50,V/V,baicalin)at the flow rate of 1.0 mL/min. The detection wavelengths were set at 265 nm (berberine hydrochloride) and 280 nm (baicalin). The column temperature was 30 ℃,and sample size was 10 μL. RESULTS:TLC spots of S. baicalensis,Crategi Fructus,L. Japonicae,Co-donopsis Radix,N. nucifera,Coptidis Rhizoma were clear and well-separated without negative interference. The linear ranges of berberine hydrochloride and baicalin were 6.67-33.34 μg/mL(r=0.9998)and 7.7-38.7 μg/mL(r=0.9999). RSDs of precision,sta-bility and reproducibility tests were all lower than 1.0% . The recoveries were 96.5% -99.9%(RSD=1.2% ,n=6)and 101.1%-102.9%(RSD=0.6%,n=6). CONCLUSIONS:Improved standard can be used for quality control of Jianpi zhixiening granules.

Objective:To analysis the predictive value of peripheral blood T-helper cells (Th17), regulatory T cells (Treg) and Th17/Treg in patients with rheumatoid arthritis (RA) comorbid cardiovascular disease.Methods:A total of 180 RA patients treated in Honghu Hospital of Traditional Chinese Medicine from January 2022 to January 2023 were selected retrospectively, they were divided into comorbidity group (72 cases) and RA group (108 cases) according to whether they had cardiovascular disease. The general data of patients were collected, the levels of peripheral blood Th17, Treg were measured by flow cytometry, the Th17/Treg ration was calculated. The risk factors of RA comorbid cardiovascular disease were analyzed by binary Logistic regression, and the predictive value of Th17 and Treg for RA comorbid cardiovascular disease was analyzed by the receiver operating characteristic (ROC) curve.Results:The levels of male proportion, uric acid, Th17, Th17/Treg ratio were increased, and Treg were decreased in the comorbidity group compared to the RA group: 63.89%(46/72) vs. 44.44%(48/108), 350.00(288.00, 404.50) μmol/L vs. 316.00 (257.50, 382.50) μmol/L, (11.28 ± 4.22)% vs. (6.60 ± 2.98)%, 3.55 ± 6.53 vs. 1.48 ± 2.54, (5.10 ± 2.19)% vs. (6.75 ± 2.86)%, there were statistical differences ( P<0.05). Binary Logistic regression analysis showed that Th17 and Th17/Treg ratio were risk factors for RA comorbid cardiovascular disease, and Treg cell ratio was a protective factor for RA comorbid cardiovascular disease (all P<0.05). The area under the curve (AUC) predicted by Th17 and Th17/Treg for RA comorbid cardiovascular disease were 0.814 and 0.785, respectively. The AUC predicted by Th17 for RA comorbid coronary heart disease, ischemic stroke and peripheral atherosclerosis were 0.804, 0.832, and 0.784, respectively. The AUC predicted by Th17/Treg for RA comorbid coronary heart disease, ischemic stroke and peripheral atherosclerosis were 0.763, 0.792, and 0.883, respectively. Conclusions:Th17, Treg and Th17/Treg are the possible influencing factors of RA comorbid cardiovascular disease, and Th17, Th17/Treg have a certain predictive value for RA comorbid cardiovascular disease.

Objective To investigate the clinical application and efficacy of DNA immune absorption in patients with lupus interstitial pneumonia.Methods to collect randomized 18 patients with lupus patients with pneumonia were enrolled in the study and randomly divided into immunoadsorption group and traditional CTX treatment group,in order to observe the ESR,CRP,ANA quantitative monitoring at different time,pulmonary function test (diffusing capacity of the lung for carbon monoxide,DLCO),6 min walking distance,procalcitonin (PCT).The difference between groups was statistically analyzed and the effect of DNA immunization was discussed.Results There were significant differences between immunoadsorption group and control group in ESR at the different time points before and after the treatment (Fgroup =7.841,P＜0.05;Fcross =6.512,P ＜0.05;Finteraction =10.421,P＜0.05),CRP(Fgroup =6.995,P＜0.05;Fcross=5.847,P＜0.05;Finteraction =8.847,P＜ 0.05) and ANA quantitative monitoring (FgrouP =12.336,P ＜ 0.05;Fcross =11.214,P ＜ 0.05;Finteraction =15.847,P＜0.05).At 1 and 2 weeks after treatment,CRP and ESR of the immunoadsorption group began to decrease,and the difference was statistically significant compared with those before treatment (P ＜0.05),while the difference between the control group and the treatment group was statistically significant after 4 weeks (P＜0.05).After 2 weeks of treatment,there was a significant difference in ANA quantitative monitoring between the immunoadsorption group,compared with that before treatment.There was a significant difference between the control group before treatment and the 6 months after treatment (P＜0.05).There was a significant difference between the immunoadsorption group and the control group in pulmonary function test (FgrouP =6.222,P＜ 0.05:Fcross =7.154,P＜ 0.05:Finteraction =8.527,P ＜ 0.05),6 min walking distance (FgrouP =8.669,P＜ 0.05;Fcross =7.154,P ＜ 0.05;Finteraction =11.547,P＜ 0.05) and PCT (FgrouP =5.621,P ＜0.05;Fcross =4.125,P ＜ 0.05;Finteraction =7.554,P ＜ 0.05.The pulmonary function and 6 min walking distance of 2-week treatment in the immunoadsorption group.There showed a significant difference compared with that before treatment.The difference between the control group after 4 weeks of treatment and that before treatment was statistically significant (P=＜0.05).There was a significant difference between the 2 weeks PCT treatment in the immunoadsorption group and that before treatment (P＜0.05).There was a significant difference between the control group after 3 months of treatment and before treatment (P ＜ 0.05).Conclusion The treatment of lupus interstitial pneumonia in traditional regimens is ineffective,and the efficacy of DNA is better than that of conventional regimens,and reduces the risk of infection.

OBJECTIVE:To establish the method for simultaneous determination of berberine hydrochloride and baicalin in Jianpi zhixiening granules. METHODS:HPLC switching walvelength method was adopted. The determination was performed on Hypersil BDS C18 column with mobile phase consisted of methanol-0.45％ phosphoric acid solution-triethylamine(50:49:1,V/V/V) at the flow rate of 1.0 mL/min. The detection wavelength was set at 265 nm(berberine hydrochloride)and 280 nm(baicalin). The column temperature was set at 30 ℃,and sample size was 10 μL. RESULTS:The linear range of berberine hydrochloride and baicalin were 60.3-312.8 ng(r=0.9997)and 81.5-368.9 ng(r=0.9999). The limits of quantitation were 0.6668,0.7740 ng,andthe limits of detection were 0.2226,0.2580 ng,respectively. RSDs of intermediate precision,stability and repeatability tests were all lower than 1.0％. The recoveries were 96.48％-99.30％(RSD=1.06％,n=6) and 95.20％-99.39％(RSD=1.66％,n=6), respectively. RSDs of durability test were all lower than 2.0％. CONCLUSIONS:The method is simple, precise, stable, reproducible,accurate and durable. It can be used for simultaneous determination of berberine hydrochloride and baicalin in Jianpi zhixiening granules.

Objective:To evaluate and summarize the best evidence for exercise scheme after bariatric and metabolic surgery in obese patients at home and abroad.Methods:We used "obesity" " bariatric and metabolic surgery" "sleeve gastrectomy" "gastric bypass surgery" "banding surgery" "exercise" "physical activity" "exercise therapy" "guideline" "expert consensus" "systematic review" "Meta-analysis" and "evidence summary" as keywords, and searched databases such as UpToDate, Cochrane Library, PubMed, China National Knowledge Infrastructure (CNKI) , Wanfang Data, and the guide network and professional association platforms by computer. Literature retrieved included guidelines, expert consensus, best clinical practice, clinical decision-making, systematic reviews, and the search time limit was from the database establishment to July 18, 2020. Two researchers evaluated the quality of literature and extracted evidence, and the evidence was revised and perfected through expert consultation.Results:A total of 4 systematic reviews and Meta-analysis articles, 2 clinical decision-making articles, 4 guidelines, and 1 expert consensus were included. The evidence was collected from 7 aspects, namely the importance of exercise, pre-exercise evaluation, exercise style, exercise intensity and duration, exercise safety, exercise follow-up, exercise effect evaluation, and finally 33 best evidences were summed up.Conclusions:Evidence of exercise scheme after bariatric and metabolic surgery in obese patients is comprehensive. Medical institutions and clinical medical and nursing staff can combine the institutional environment to transform and apply the evidence.

Acute high altitude disease (AHAD) is a general term for a series of clinical reactions that occur when the body fails to adapt to the low-pressure hypoxic environment of high altitudes. Mild cases can cause symptoms such as headache, nausea and vomiting, while more severe cases can lead to life-threatening conditions such as pulmonary edema, cerebral edema and other critical conditions that can be fatal. With the increasing demand for high altitudes deployment, understanding the common preventive measures of AHAD can reduce its morbidity or mortality to a certain extent, which is of great benefit to those who reside temporarily at high altitudes. In recent years, as people's health awareness has improved, there has been a growing attention towards non-pharmacological methods of disease prevention. At the same time, non-pharmacological therapy has significant therapeutic effects in preventing and treating high-altitude diseases, which has attracted the attention of researchers in this field. This review summarizes the major non-pharmacological preventive components of modern medicine and outlines the current non-pharmacological approaches to AHAD from the perspective of traditional Chinese medicine, intending to serve clinical purposes and improve the onset and prognosis of AHAD.

Objective:To establish the quality evaluation method of Perillae caulis formula granules based on the three kind of quality indexes of standard decoction. Methods:Eighteen batches of Perillae caulis were collected from different habitats according to different technical requirements, eighteen batches of standard decoction and three batches of formula granules were prepared and the paste-forming rates were calculated. The content of Caffeic acid and Rosmarinic acid were determined and calculated by Ultra High Performance Liquid Chromatography (UPLC). Then the fingerprints of standard decoction of and formula granules of Perillae caulis were established by UPLC . The similarity values of fingerprints between formula granules and standard decoction were calculated. Results:The average paste-forming rate of standard decoction was (7.16±1.97)%. The paste-forming rates of three batches of formula granules were 5.52%, 5.25% and 5.34%, respectively. The average content of Caffeic acid and Rosmarinic acid in standard decoction was (12.06±3.37)mg/g. The contents of three batches of formula granules were 5.52, 5.82, 5.77 mg/g, respectively. Seven common fingerprint peaks were identified in the fingerprints of standard decoction and formula granules, three of which were identified as Caffeic acid, N-Feruloyl Octopus amine and Rosmarinic acid by comparison of reference substance. The fingerprints similarity of Perillae caulis dispensing granules and standard decoction were 1.000, 0.995 and 0.997, respectively. Conclusions:The quality indexes of three batches of formulation granules are consistent with standard decoction. This method can provide basis for the establishment of quality standard of Perillae caulis dispensing granules.