The results showed that long-term and non-pulsatile administration of high doses of GnRH-A made in China produced a reversible inhibition of testicular endocrine function in male rats, resulting in decrease of testosterone level and increase of progesterone level both in sera and culture media. Besides, there was a marked decrease in the weight of their seminal vesicles and ventral prostates. It was found that GnRH-A we used had no effect on testis weight, the structure of seminiferous tubules and the production of estrogen by cultured Sertoli cells, and no serious side effect, either. It suggested that home made GnRH-A may be useful in the treatment of sex hormone-dependent diseases as reported in foreign literature.

Objective To investigate the pathological staining method of pulmonary cryptococcal disease. Methods Traditional hexamine silver staining and acidified potassium permanganate solution hexamine silver stained were used to analyze paraffin sections of 51 patients with pulmonary cryptococcal disease. Results Compared with traditional hexamine silver stained, the result of acidified potassium permanganate solution with hexamine silver stained was positive, and the whole process only required 25 minutes with the clear background and cryptococcal cyst capsule structure. Conclusion Compared with the traditional hexamine silver staining, acidification of potassium permanganate solution hexamine silver staining method is simpler,easier,more effective and worthy of wide application.

Monkeypox is a zoonotic disease.Previous studies have shown that children are vulnerable to monkeypox and are also at high risk for severe disease or complications.In order to improve pediatricians′ understanding of monkeypox and achieve early detection, early diagnosis, early treatment and early disposal, the committee composed of more than 40 experts in the related fields of infectious diseases, pediatrics, infection control and public health formulate this expert consensus, on the basis of the latest clinical management and infection prevention and control for monkeypox released by the World Health Organization (WHO), the guidelines for diagnosis and treatment of monkeypox (version 2022) issued by National Health Commission of the People′s Republic of China and other relevant documents.During the development of this consensus, multidisciplinary experts have repeatedly demonstrated the etiology, epidemiology, transmission, clinical manifestations, laboratory examinations, diagnosis and differential diagnosis, treatment, discharge criteria, prevention, case management process and key points of prevention and control about monkeypox.