BACKGROUND:Polyurethane has good mechanical and physical characteristics and is extensively used in
clinical and experimental studies, but its hydrophobicity and histocompatibility are not ideal, which limits its use in tissue engineering as a biomaterial scaffold to some extents.
OBJECTIVE:To observe the hydrophilicity of polyurethane membrane grafted with silk fibroin and glutin and its compatibility with human hypopharyngeal cel s.
METHODS:The changes in hydrophilicity of polyurethane membrane grafted with silk fibroin and glutin were detected by contact angle measurements. Human hypopharyngeal fibroblasts were cultured in vitro on
polyurethane membrane, silk fibroin-polyurethane membrane, glutin-polyurethane membrane and tissue culture plate. Cel compatibility was compared using cytometry and cel morphology obsevation.
RESULTS AND CONCLUSION:Hydrophilicity of silk fibroin-or glutin-polyurethane membranes significantly increased (P<0.01). The hydrophilicity of silk fibroin-polyurethane membrane was higher than that of
glutin-polyurethane membrane (P<0.01). The number of cel s on the tissue culture plate was the most. The number of human hypopharyngeal fibroblasts on the silk fibroin-or glutin-polyurethane membrane was higher
than that on the polyurethane membrane, especial y on the silk fibroin-polyurethane membrane. These suggested that hydrophilicity and cel compatibility of silk fibroin-or glutin-polyurethane membrane were elevated.

OBJECTIVE: To investigate into the surgical approaches and clinical curative effect of peripheral facial nerve paralysis in different causes and injury location. METHOD: Thirty-two cases of peripheral facial paralysis were treated with selective facial nerve decompression via different surgical approaches. After 0.5 year to 2 years follow up, the recovered functions of facial nerve were judged by House and Brackmann grading system. RESULT: In all patients, 17 cases of peripheral facial paralysis caused by trauma were underwent facial nerve decompression in two weeks, 11 cases reached degree I (84.6%) according to House-Brackmann (H-B) facial nerve function scores, 3 cases were treated in the third week, 2 cases reached degree II and other 1 cases reached degree III. One patient was treated after 8 weeks and facial nerve function scores only reached degree VI. Thirteen cases of otomastoiditis in middle ear were underwent facial nerve decompression in one week, postoperative House-Brackmann grade was I in 8 cases, II in 2, and III in 4. CONCLUSION Majority of patients with peripheral facial paralysis were treated with facial nerve decompression have better clinical curative effect by suitable operation and juncture due to trauma or cholesteatoma otitis media, but operation for traumatic peripheral facial paralysis should be undertaken in two weeks.
Adolescent ; Adult ; Cholesteatoma, Middle Ear ; surgery ; Decompression, Surgical ; methods ; Facial Nerve ; surgery ; Facial Paralysis ; surgery ; Female ; Follow-Up Studies ; Humans ; Male ; Middle Aged ; Temporal Bone ; surgery ; Young Adult

OBJECTIVE: To study the significance of exposure of the recurrent laryngeal nerve (RLN ) in thyroid gland surgery. METHOD: Three hundred and thirty-two thyroidectomy cases were studied from January 2008 to June 2011. All patients had general anesthesia, and RLN were exposed during operation. One hundred and thirty-one cases were operated with total lobectomies, 138 cases with subtotal thyroidectomy, 51 cases with total lobectomies and contralateral subtotal thyroidectomy, 12 cases with total thyroidectomy. RESULT: Five hundred and thirty-three RLNs were exposed, 4 cases came with hoarseness postoperatively, All cases recovered within 3 months. CONCLUSION Exposure the recurrent laryngeal nerve in the thyroidectomy was available and could protect RLN.
Adolescent ; Adult ; Aged ; Female ; Hoarseness ; surgery ; Humans ; Male ; Middle Aged ; Recurrent Laryngeal Nerve ; surgery ; Retrospective Studies ; Thyroidectomy ; methods ; Young Adult

Objective To investigate the relationship between the expression level of miR-196a in plasma and the clin-icopathologic factors of HSCC. Methods Paired plasma specimens from HSCC patients and 50 healthy people were collected. Plasma total RNA was extracted from 57 HSCC patients and 50 healthy people ,and the expression level of miR-196a was measured by quantitative real time PCR. The clinicopathological features between the plasma expression level of miR-196a were analyzed. ROC curve were constructed to evaluate the diagnostic value. Results miR-196a exhi-biting higher expression in pre-operation HSCC plasma with △Ct=9.11(5.69,12.43)compared with post-operation HSCC plasma with△Ct=12.69(9.16,14.84)and healthy group with△Ct=12.85(11.18,14.91)(P<0.01). There were no significant differences between the expression level of miR-196a in healthy group and post-operation group (P>0.05). miR-196a levels in plasma were correlated with grade of differentiation (P<0.05), lymph node metastasis(P<0.01), and clinical stage(P<0.05), while there was no relationship with age and smoking history(P>0.05). The areas under the ROC curves were 0.755. The cut-off point of△Ct was 10.84,with the 95%CI=0.66-8.85(P<0.01). Conclusion Plasma expression level of miR-196a have realationship with clinical stage ,lymph node matastasis and grade of differentiation. miR-196a may have involved in carcinogenesis and tumor development of HSCC. Plasma miR-196a could be potential minimally invasive biomarkers for HSCC patients.

Objective To explore the clinical effects of eustachian tube insufflation via nasal endoscopy combined with administration of hormone and antibiotics in treating secretory otitis media (SOM). Methods A total of 65 patients with SOM who were admitted to our hospital from January 2013 to October 2014 were selected. They were randomly allocat-ed to an observation group (33 patients) and a control group (32 patients) according to their conditions. The observation group was given eustachian tube insufflation via nasal endoscopy combined with oral administration, while the control group was given oral administration. After one-month treatment, clinical effects, changes of tympanogram, airway aver-age threshold measured by pure tone audiometry testing, complications and adverse events between the two groups were observed. Results The difference of total effective rate in the two groups (90.24%vs 62.16%) was statistically significant (P<0.05);proportion of type A recovery of tympanogram in the two groups was compared (53.66% vs 29.73%), and the difference was statistically significant (P<0.05); after the treatment, results of pure tone audiometry testing in the two groups (20.63±2.52 vs 26.92±2.95) were statistically significant (P<0.05);one case of one-time dizziness was seen in the observation group;relapse rates in the two groups (4.88%vs 13.51%) were not statistically significant (P>0.05). Conclu-sion Eustachian tube insufflation via nasal endoscopy combined with oral administration has definite clinical effects on SOM, which is worthy of clinical promotion.

Objective To study classification of vascular sign of tiny ground glass nodules-like lung adenocarcinoma in HRCT, and explore its value to differentiate benign from malignant of the ground glass nodules(GGN).Methods 87 patients with tiny ground glass nodules-like lung adenocarcinoma examined on HRCT were retrospectively evaluated.According to the new pathological classification standard of lung adenocarcinoma,they were divided into three groups:(1)pre-invasive group(n=25),including 14 cases of atypical adenomatous hyperplasia(AAH)and 11 cases of adenocarcinoma in situ(AIS);(2)minimally invasive adenocarcinoma (MIA)group(n=35);(3)invasive adenocarcinoma(IAC)group(n=27).The lesions were divided into three types according to the grinding of glass composition proportion:Type A,pure ground glass nodules(pGGN);Type B,mixed ground glass nodules (mGGN)which contained glass composition≥50%;Type C,mGGN which contained glass composition < 50%.The vascular sign of GGN were divided into four types:Type 1,without vessels passing through the GGN,or vessels passing by GGN;Type 2,intact vessels passing through GGN,but vascular morphology is normal;Type 3,single vessels passing through GGN,and distorted,stiff vessels seen within GGN;Type 4,two or more vessels passing through GGN,and branches between vessels formed in GGN,and the diameter of vessel was irregular,partial enlargement.The relationship among the size of the GGN,content of the grinding of glass proportion and the vascular sign of GGN were analyzed both in axial images and reconstruction images.Results There were significant differences in size among the three groups(P=0.032,P=0.000,P=0.000).There were significant differences between content of the grinding of glass proportion and classification of vascular sign of GGN(P=0.000).Type 1 and type 2 vascular sign were dominant in the infiltrating former group,a total of 24 cases(96%).The incidence of type 3 and type 4 vascular sign in MIA group and IAC group was 60% and 74% respectively,and there were significant differences with the infiltrating former group(P=0.000,0.000).Further analysis indicated that type 3 was more commonly seen in MIA with comparison to type 4 which was more likely seen in IAC,the difference was statistically significant(P=0.043).Conclusion To study HRCT vascular sign of tiny ground glass nodules-like lung adenocarcinoma can improve the ability of the GGN benign and malignant diagnosis,provide reliable basis for clinical diagnosis and treatment.

Objective To investigate the effects of microRNA-519b-3p (miR-519b-3p) on laryngeal carcinoma Hep-2 cell growth,and to analyze the underlying molecular mechanisms.Methods The effects of miR-519b-3p on the growth and cell cycle of Hep-2 cells transfected with miR-519b-3p mimic were tested by MTT assay and flow cytometry,respectively.The mRNA and protein expressions of the related genes were tested by reverse transcription polymerase chain reaction (RT-PCR) and Western blot,respectively.The expressions of miR-519b-3p were tested by real-time RT-PCR in 48 pairs of laryngeal carcinoma and adjacent tissue samples.Results The expression of miR-519b-3p in laryngeal carcinoma tissues was significant lower than that in adjacent non-cancerous tissues (SAct =2.989,t =2.693,P ＜ 0.01).Increasing the level of miR-519b-3p inhibited significantly Hep-2 cell proliferation,arrested the cell cycle in the G2/M phase (10.29％ ± 4.63％,t =4.395,P ＜ O.05),and decreased significantly the percentage of cells in the S phase (7.56％-± 2.05％,t =3.555,P ＜ O.05),with the increase in the expression of cyclin dependent kinase (CDK) 1 and the decrease in the expressions of CDK 2 and Cyclin A.RT-PCR and Western blot showed that miR-519b-3p down-regulated the protein but not mRNA expressions of HuR and cyclooxgenase-2 (COX-2) genes.Conclusions The expression of MiR-519b-3p as carcinoma suppressor gene is low in laryngeal carcinoma.The cell cycle of Hep-2 cells was arrested in the G2/M phase by MiR-519b-3p.

Objective:To explore the surgical efficacy of conductive deafness caused by otosclerosis and ossicular malformation with 980 nm fiber laser stapedial floor fenestration.Methods:Data of 58 patients (ears) who were diagnosed with conductive deafness caused by otosclerosis (49 ears) and ossicular malformation (9 ears) treated by 980 nm Diode laser small-fenestra stapedotomy were retrospectively analyzed. Air conduction (AC) thresholds, bone conduction (BC) thresholds, and air-bone gap (ABG) at 0.5, 1, 2, 4 kHz pure tone frequencies were compared before and 3 months after surgery, and the advantages and precautions of 980 nm fiber laser were summarized. Paired t-test (SPSS 26.0 software) was use to analyze the listening data. Results:Fiber optic laser stapes fenestration and artificial stapes implantation were successfully completed in all 57 cases (ears), the hearing of another one patient (ear) with floating malformation of detachment of stapedial floor was lower than that before surgery. Preoperative at 0.5, 1, 2, 4 kHz frequencies of AC thresholds, BC thresholds, and ABG were (65.4±9.7) dB, (27.2±8.9) dB, and (38.2±9.8) dB respectively. Postoperative 3 months at the same frequency of AC thresholds, BC thresholds, and ABG were (42.1±11.3) dB, (26.9±6.6) dB, and (15.2±9.1) dB. Preoperative and postoperative of AC threshold and ABG were statistically significant at 0.5, 1, 2, 4 kHz ( t value was 13.270 and13.948, both P<0.01), and yet the BC threshold was not statistically significant before and after surgery at the same frequency ( t=0.418, P>0.05). Conclusions:980 nm fiber laser stapes floor fenestration is an effective treatment for conductive deafness caused by otosclerosis and ossicular malformation.

Hyaluronic acid (HA) is an important biomaterial as the extracellular matrix in human body. We produced HA by fermentation of Streptococcus iniae (Strep.). Production of HA by Strep. was evaluated and further improved by strain mutation by ultraviolet. One strain with higher HA yield and lower content of protein was obtained. Its HA yield increased from (82.3±3.3) mg/L to (120±10.6) mg/L, and protein decreased from (0.178±0.011) mg/L to (0.032±0.017) mg/L. The molecular weight (MW) of HA yield from Strep. is about 3.0×10⁵ Da. Using the method of freezing and thawing, HA aqueous solution was transferred into hydrogel. This HA hydrogel, casted on sterilized non-woven fabric, was applied to repair rabbit skin with full-thickness defect. The preliminary results of the animal tests displayed that HA hydrogel obviously reduced the inflammation around the wound and promoted the skin regeneration comparing with the control tests.
Animals ; Biocompatible Materials ; Fermentation ; Hyaluronic Acid ; biosynthesis ; Hydrogels ; Molecular Weight ; Rabbits ; Regeneration ; Skin ; drug effects ; growth & development ; Streptococcus iniae ; metabolism ; Wound Healing

To investigate the effect of silencing DJ-1 on xenografted human laryngeal squamous cell carcinoma (LSCC) Hep-2 cells in nude mice.Xenograft model of human LSCC was established by subcutaneous transplantation of Hep-2 cells in 24 nude mice. The LSCC-bearing nude mice were randomly divided into 3 groups (=8 in each):DJ-1 siRNA low dose group and DJ-1 siRNA high dose group were injected in tumors with 20 μg of DJ-1 siRNA or 40 μg of DJ-1 siRNA in 50 μL, respectively; control group was injected with 5% glucose solution in 50 μL, twice a week for 3 weeks. The weight and size of tumors were measured before injection. The animals were sacrificed 48 h after the final treatment, and the tumors were harvested and weighed. The apoptosis and proliferation of tumor cells were determined; the expressions of Caspase-3 and Ki-67 in tumor specimens were detected with immunohistochemistry. The expression of DJ-1, PTEN, survivin mRNA and protein in tumor tissues were detected by RT-PCR and Western blotting, respectively.Tumor weight in low dose group[(0.66±0.15)g] and high dose group[(0.48±0.11)g] were significantly lower than that in control group[(0.83±0.16)g, all<0.05]. The inhibition rates of low dose group and high dose group were (20.48±0.18)% and (42.16±0.13)%, respectively. Immunohistochemistry showed that the expression of Caspase-3 was increased and Ki-67 was reduced in tumor specimens, compared with the control group (all<0.05). RT-PCR and Western blot results showed that in low dose group and high dose group the mRNA and protein expression of DJ-1 and survivin significantly decreased (all<0.05), while PTEN mRNA and protein content increased (all<0.05).High dose DJ-1 siRNA can inhibit the tumor growth in human LSCC xenograft nude mouse model, which indicates that down-regulating DJ-1 and survivin, and up-regulating PTEN expression may lead to blockage of PI3K-PKB/Akt signaling pathway and promoting tumor cell apoptosis.
Animals ; Antineoplastic Agents ; pharmacology ; Apoptosis ; drug effects ; genetics ; Carcinoma, Squamous Cell ; chemistry ; genetics ; physiopathology ; Caspase 3 ; analysis ; drug effects ; Cell Line, Tumor ; chemistry ; drug effects ; physiology ; transplantation ; Cell Proliferation ; drug effects ; Down-Regulation ; Gene Expression Regulation ; drug effects ; genetics ; physiology ; Head and Neck Neoplasms ; chemistry ; genetics ; physiopathology ; Heterografts ; drug effects ; physiology ; Humans ; Inhibitor of Apoptosis Proteins ; analysis ; drug effects ; Ki-67 Antigen ; analysis ; drug effects ; Laryngeal Neoplasms ; chemistry ; genetics ; physiopathology ; Mice, Nude ; PTEN Phosphohydrolase ; analysis ; drug effects ; Phosphatidylinositol 3-Kinases ; drug effects ; Protein Deglycase DJ-1 ; pharmacology ; Proto-Oncogene Proteins c-akt ; drug effects ; RNA Interference ; physiology ; RNA, Messenger ; pharmacology ; RNA, Small Interfering ; physiology ; Signal Transduction ; drug effects ; genetics ; physiology