Doxorubicin is widely used in chemotherapy of breast cancer and many other cancers.How ever,doxorubicin resistance restricts its use to some extent.The mechanisms of doxorubicin resistance may involve transport protein,apoptosis protein,DNA repair function,enzyme and other factors.Researches about reversing doxorubicin resistance are ongoing and provide some effective therapeutic regimens to overcome doxorubicin resistance clinically.But the specific mechanism of doxorubicin resistance is yet to be elucidated.It is expected that more reasonable and effective counter-measures will be put forward to improve the theraputical effect of doxorubicin.

Objective To establish a method for the determination of hesPeridin and cinnamaldehyde in siweiqing buccal tablets. Methods A high Performance liquid chromatograPhy ( HPLC) method was established. The chromatograPhic conditions were as follows: the chromatograPhic column Inertsil ODS_SP ( 4. 6 mmí150 mm, 5 μm ) with the mixture of acetonitrile and water as mobile Phase in gradient mode,the detection waVelength of 290 nm,the flow rate of 1. 0 mL·min-1 ,the column temPerature of 30℃,the theoretical Plate number no less than 3 000. Results The linear relationshiP between content and Peak area was obtained in the range of 0. 025-0. 500 μg for cinnamaldehyde and 0. 10-2. 00 μg for hesPeridin. RSD of the Peak area of cinnamaldehyde and hesPeridin for the Precision test,the stability test and the rePeatability test were 1. 16%and 1. 03%,1. 27%and 1. 08%,1. 23%and 1. 28%,resPectiVely. The aVerage recoVery of cinnamaldehyde was 99. 24%with RSD of 1. 65%(n=9). The aVerage recoVery of hesPeridin was 99. 39%with RSD of 1. 85%(n=9). Conclusion The method can be aPPlied to quantitatiVe assay of cinnamaldehyde and hesPeridin in siweiqing buccal tablets with good reProducibility and sPecificity. The recoVery result met the accePtance criteria. The method can effectiVely control the quality of siweiqing buccal tablets with good sPecificity,reProducibility and stability,and can be aPPlied to the quality control of siweiqing buccal tablets.

Objective To observe the effect of external fixator combined with limited internal fixation in the treatment of complex ankle fractures.Methods According to the digital table,65 patients with complex ankle fracture were randomly divided into treatment group(33 cases) and control group(32 cases).The treatment group was treated with external fixator combined with limited internal fixation,the control group was treated with internal fixation.All patients were followed up for 5 to 20 months.The curative effect and incidence rate of complication of the two groups were observed.Results The excellent rate of the treatment group was 84.85%,which was higher than 62.50% of the control group,the difference was statistically significant(x2=4.20,P<0.05).The incidence rate of complications in the treatment group was 3.03%,which was lower than 18.75% of the control group,the difference was statistically significant(x2=4.18,P<0.05).Conclusion External fixator combined with limited internal fixation can effectively treat complex ankle fractures,the method can not only make patients satisfied with the reduction effect,but also the internal fixation complications such as infection are minimized,the clinical value is extremely high,it is worthy of wide application.

This study is to report the preparation of complexes of Ad5 and anionic liposomes (AL-Ad5), the amplification of adenoviruses with enhanced green fluorescent protein (eGFP) reporter gene performed by HEK 293 cells, the adenoviral vectors purified by cesium chloride gradient centrifugation, and the titer of adenovirus determined by cytopathic effect (CPE) method, hexon capsid immunoassay and quantitative-PCR (Q-PCR), separately. The prescription and experiment conditions were optimized by central composite design (CCD). The complexes of Ad5 and AL-Ad5 were formulated by the calcium-induced phase change method. The morpholopy, particle size and zeta potential were detected by dynamic light scattering (DLS) and transmission electron microscopy (TEM), respectively. Additionally, the bicolourable fluoresce-labeled complexes (F(labeled)-AL-Ad5) were prepared and their intracellular location in MDCK cells was detected by confocal laser scanning microscopy (CLSM). The results indicate that the complexes of AL-Ad5 exhibited a uniform distribution with a particle size of 211 +/- 10 nm and a zeta potential of -41.2 +/- 2.2 mV. The result of CLSM demonstrates that the intracellular location of red fluoresce-labeled adenovirus was consistent with that of green fluoresce-labeled liposomes suggesting that the naked adenovirus was well encapsulated by the anionic liposomes in complexes of AL-Ad5.

Recent studies in the realm of metabolic chemistry of traditional Chinese drugs (TCD),appeared in literature were reviewed. Methods for such study were enumerated and commented upon.Some problems that arised in the study were discussed. It was suggested that the study of metabolic chem-istry on TCD and its compounded preparations should be further pursued.

Objective To analyze strain characteristics of hypertrophic cardiomyopathy(HCM) and explore the clinical value of strain rate imaging in differentiating HCM from hypertensive left ventricular hypertrophy(H-LVH). Methods Parameters of strain and strain rate were obtained and compared among 23 HCM patients, 30 patients with H-LVH and 20 healthy people(control group). Results In HCM group, systolic strain(?_ sys ),peak systolic strain rate(SR_ sys ), early diastolic strain rate(SR_ dia ) obtained from inter-ventricle septum(IVS) were smaller than that obtained from left ventricle posterior wall(LVPW), while SR_a increased in IVS. ?_ sys ,SR_ sys ,SR_ dia in both patients with HCM and H-LVH were smaller than that in control group, while atrial systolic strain rate(SR_a) increased in HCM group and H-LVH group. ?_ sys , SR_ sys , SR_ dia , SR_a obtained from IVS and ?_ sys , SR_ dia obtained from LVPW in HCM group showed statistic differences compared with H-LVH group. Conclusions Strain rate imaging could accurately detect the abnormal regional myocardial function of HCM and can be applied as a useful tool in discriminating HCM from H-LVH.

Objective To establish a method for determination of total soluble solids (TDS) in self-provided well water .Methods The conductivity meter which has a function of determination of total soluble solid ,and was used to directly determinate the total soluble solids in self-provided well water .Results Compared with national standard method ,the results have no significant differ-ence .The relative standard deviation (RSD) was 0 .17% -0 .22% ,and the recovery rates of standard was 97 .5% -98 .6% .Conclu-sion The method is quick ,simple ,sensitive ,accurate and suitable for rapid determination of large quantities of waters .

Aseptic loosening of the prosthesis due to periprosthetic osteolysis is one of the main causes that affect the service life following artificial joint replacement. The patient should undergo revision of artificial joints,but the surgery is of high difficulty,high risk and excessive cost,etc. Therefore,using nonsurgical technique to control artificial prosthesis osteolysis is an urgent and realistic task. Currently,the drug prevents osteolysis through inhibiting osteoclast bone resorption,responding to cytokines,and promoting bone formation. In addition,gene therapy and other treatments for osteolysis are under exploration.

[Objective]To detect the effect of Etanercept on the Ti particles induced TNF-?IL-1 and IL-6 production by macrophage and to evaluate the validity of the etanercept on treatment of aseptic loosening of prosthesis.[Method]Separate and Cultivate mouse peritoneal macrophages were divide into 5 groups after 24 h.Group A was treated with M? alone,group B with M?+Ti particles,group C with M?+Ti particles + etanercept(10 ng/ml),group D with M? Ti particles + etanercept(100 ng/ml),group E with M? + Ti particles + etanercept(1000 ng/ml).After 18 h,the production of TNF-? IL-1 and IL-6 in culture supernatants was detected by ELISA.[Result]The levels of TNF-? IL-1 and IL-6 production were much higher in group B than those in group A,D,E(P0.05).[Conclusion]Is shows the Ti particles could stimulate M? to excrete profuse TNF-? IL-1 and IL-6 production,etanercept can significantly inhibited the production of TNF-? IL-1 and IL-6 secreted by Ti particles induced macrophages in a dose-dependent manner and hope to be a therapeutic candidate for the prevention of aseptic loosening.