Objective To study the feasibility of LDRf classification for gastrointestinal tract ectopic varices (EcV) outside the esophagus.Methods Data of 914 patients with gastrointestinal EcV were classified by LDRf and analyzed for EcV location (L),vascular diameter (D),and risk factor (Rf).The etiology of the portal hypertension (PH) was determined,and the patients were treated and followed up.Results The EcVs were located in duodenum of 198 cases,in jejunum and ileum of 93,in bile duct of 105,in colon of 65,and in rectum of 453.Diameters of blood vessels of EcV varied from 0.3 to 3.5 cm.PH causes were cirrhosis with portal hypertention in 630 patients (68.9％),in which 3 were autoimmune cirrhosis and 3 were portal spongiform liver disease (0.6％).Combined esophageal and gastric varices were found in 252 cases (27.6 ％),including 4 cases (0.5％) of splenectomy.Various treatments were applied in 315 patients,including endoscopic tissue adhesive injection in 43,endoscopic sclerotherapy in 76,endoscopic ligation in 74,interventional treatment in 52,and surgical laparotomy in 70.A total of 19 patients died of variceal bleeding.The patients were followed up for 13 to 36 months,no varices relapsed,and 1-year survival rate was 100％.Conclusion LDRf classification,simple,applicable,standardized,is suitable for the whole gastrointestinal varicose veins.

Objective To explore the influence of porcine esophageal variceal pressure on complete ligation for polycyclic ligator in vitro.Methods The experimental porcine venous vessels were selected to make the models of different venous pressure,which were divided into 3 groups according to the preset pressure range,P1 group 25-30 cmH2O(1 cmH2O =0.098 KPa),P2 group 35-40 cmH2O,P3 group 45-50 cmH2O.Ligation effects of each group were analysed.Results There were 75 complete ligations (47.77％,75/157) in group P1,41 in group P2 (32.28％,41/127),and 29 in group P3 (23.58％,29/123).There were significant differences among the three groups (x2 =19.558 5,P =0.000 6).Conclusion The higher the variceal pressure is,the worse the effect of ligation is.Variceal pressure could predict the effect of endoscopic ligation,and is helpful for the choice of endoscopic treatment.

Objective To investigate the effect of bone marrow mesenchymal stem cells (bone marrow stem cells,BMSCs) through different approaches in the treatment of transplanted cirrhotic model in rabbit effect difference.Methods Establishment of rabbit model of carbon tetrachloride cirrhosis with liquid olive oil mixture of subcutaneous inj ection and ethanol fed,the liver cirrho-sis models were randomly divided into cirrhosis model group (n=10),hepatic artery transplantation group (n=10),ear vein trans-plantation group (n=10).Healthy New Zealand rabbits bone marrow,using the method of whole bone marrow adherent cultured bone marrow BMSCs,and then were transplanted and serum albumin,alanine aminotransferase in 3 ways;by HE staining,the de-gree of hepatic cirrhosis and detection of serum albumin by Western blot expression in liver.Results Through the vein group and ar-tery transplantation group BMSCs to cirrhotic model in rabbit,4 weeks after the discovery of liver transplantation group rabbits were significantly improved,serum albumin is liver cirrhosis without transplantation group increased significantly(P<0.05),transami-nase is liver cirrhosis without transplantation group decreased significantly (P<0.05).The therapeutic effect of 2 ways of BMSCs transplantation on rabbit liver cirrhosis had a significant difference (P<0.05).Conclusion Transplantation of BMSCs through the ear vein and the hepatic artery of rabbit after 4 weeks of treatment of liver cirrhosis,liver function can be improved.But the hepatic artery transplantation therapy is obviously better than that of intravenous transplantation of treatment effect.

Objective To explore the influence of different pressures and diameters in vitro on complete ligation degree of esophageal varices based on the principle of LDRf typing.Methods According to the different preset venous pressure,the experimental porcine veins were used to establish vascular pressure models and divided into three groups (group A 25-30 cmH2O,group B 35-40 cmH2O,group C 45-50 cmH2 O).The porcine esophagus was selected to establish models of esophageal varices,and divided into three groups according to the diameter of esophageal varices (group D1 0.4-1.0 cm,group D2 1.0-1.5 cm,group D3 1.6-2.0 cm).Influence of pressure and diameter on complete ligation degrees was evaluated by univariate and multivariate analysis.Results Group A completed 18 ligations (56.25％,18/32),group B 12 ligations (37.50％,12/32),and group C 11 ligations (33.33％,11/33),which were not significantly different by pressure (x2 =3.6126,P =0.0573).Group D1 completed 35 ligations (94.59％,35/37),group D2 6 ligations(16.67％,6/36),and D3 completed no ligation (0％,0/24),which were significantly different by diameter (x2 =38.0014,P =0.0000),indicating that the diameter of the varices exerted greater influence on the ligation degree.The logistic regression analysis showed that variceal pressure and diameter were the independent factors for complete ligation degree (P =0.000).Conclusion Complete ligation is effective and safe when the variceal diameter is 0.4-1.0 cm,or variceal pressure is 25-30 cm H2O.Endoscopic treatment for varices with the guide of LDRf typing is scientific and feasible.

Objective To explore the effect of non-directed musical therapy on the automatic thoughts and therapeutic effect of depression.Methods 60 people outpatient and inpatient who with depression disorder participated in this research.According to the medical order,we choose the people who with Cardinality order as the group of the musical therapy (study group) and the people with the even order as the group of drug therapy (control group).all the patients was test with the Hamilton's Depression Scale (HAMD-24) and Automatic Thoughts Questionnaire (ATQ) before and after 2,4,8 weeks treatment,and comparison of these questionnaires scores of the two groups.Results After the 2,4,8 weeks treatment,the HAMD scores of the study group was (15.70±2.63),(10.03±2.28),(6.00± 1.98) and the scores of control group was (17.43±2.62),(12.47±2.75),(7.50±2.03).The result of HAMD scores had significant difference(F=11.754,P=0.001).ATQ scores of the study group was (73.27± 17.60),(58.33± 13.66),(47.43± 11.94) and the scores of control group was(84.20±22.30),(72.53±20.86),(58.90± 18.61).The result of ATQ scores had significant difference(F=4.136,P=0.047).Conclusion Non-directive musical imagery could be an effective way to improve the automatic thoughts of people who with depression disorder and improve the effect of treatment.

Objective To investigate the feasibility of Chelex DNA extraction from thin blood smears for genetic analysis, and to develop smear-based nested polymerase chain reaction (PCR) of the 18S RNA of Plasrnodium falciparum. Methods Chelex-100 which was chelating ion exchange resin was used to extract DNA from Giemsa-stained or unstained thin blood smears of different concentrations of Plasmodium falciparum. With the extracted DNA as the template, 18S RNA gene was amplified by nested PCR to test the susceptibility of Chelex method. Results Positive band of 205bp appeared in nested PCR with DNA extracted from Giemsa-stained or unstained thin blood smears of patient with falciparum malaria. Using the Chelex method, the detection limits of the smear-based nested PCR were 1.5 × 101 parasite/μL blood for Giemsa-stained and 1.5×10-1 parasite/μL blood for unstained thin blood smears. Conclusions Chelex DNA extraction is a simple and efficient method for extracting trace amount of DNA from thin blood smear. The smear-based nested PCR developed in this study is feasible to identify the gene from reserved thin blood smears and will provide a new approach for clinical diagnosis and study of molecular epidemiology.

Objective To investigate the changes of carotid, lipidemia and fibrinogen in type 2 diabetes mellitus patients complicated with cerebral infarction (DCI). Methods 58 cases of DCI (DCI group) were enrolled in this study, and 36 cases of cerebral infarction without diabetes mellitus served as control group. The location, number and nature of carotid atherosclerotic plaque and inner diameter of carotid artery were assessed by Doppler ultrasonography in all the patients, as well as blood lipid, blood glucose and fibrinogen were evaluated. Results In DCI group, the rate of carotid atherosclerotic plaque was 79.3%, the IMT and inner diameter of common carotid artery in DCI group were significantly different from control group (all (P

Objective To investigate the value of renal CT arteriography in the preoperation evaluation of patients with renal tumors.Methods From Jan 2007 to June 2012,372 patients with renal tumors took renal CT arteriography (with Philips Brilliance 16) to evaluate the variations of their renal arteries.We executed plain scan first,then artery phase,venous phase scan and delaying scan by turns after injections of Omnipaque.All images were delivered to imaging workstation and reconstructed to 3D views of renal arteries to supply imageological supports for surgeons.Results Tumors in solitary kidneys were detected in 21 patients,and masses in both kindneys were detected in 4 cases.We collected data of 376 kidneys finally.We found premature branching renal arteries on 46 kidneys (12.2％) and accessory renal arteries on 74 kidneys (19.7％).Double accessory renal arteries were seen on 10 kidneys (2.7％),and there were 84 accessory renal arteries in summary.7 accessory renal arteries (7.2％) came from arteria phrenica and 77 (91.7％) came from abdominal aorta (x2 =65.68,P ＜ 0.01).We found the regions where the accessory renal arteries entered kidneys:65.5％ (55/84) in upper poles,19.0％ (16/84) in central regions and 15.5％ (13/84)in inferior poles.Routine CT scans found 44 variations of renal arteries in 218 cases who underwent surgeries,and then renal CT arteriography found 63 variations in the same cases (20.2％ vs 28.9％,x2 =4.47,P ＜ 0.05).Finally,surgeons found 60 variations of renal artries in operations in the 63 cases with variations found by renal CT arteriography before surgeries.Conclusions There is limitations of routine CT scan (plain scan and with contrast medium) in detection of renal artery variations.Renal CT arteriography can supply reliable supports for establishment of surgical plans for renal tumors.

Objective To explore the efficacy of percutaneous intratumoral injection of mixed emulsion with hot lipiodol and pira-rubicin for treatment of liver metastases lack of blood supply.Methods 12 patients with liver metastases,confirmed by the clinical and pathological examination,were treated by intratumoral injection of mixed emulsion with heating lipiodol and pirarubicin using 21G biliary needle under CT guidance.The adverse events were recorded and the treatment efficacy was also assessed according to the imaging findings.Results 8 patients achieved a partial remission of lesions,and 3 ones had stable condition.No serious adverse reactions occurred.Conclusion It is effective,simple,convenient and safe for treatment of liver metastases lack of blood supply with percutaneous intratumoral injection of emulsion with hot lipiodol and pirarubicin.

Objective To explore the association of the Arg64 polymorphism β-3-adrenergic receptor(β3-AR) gene with polycystic ovary syndrome(PCOS)and its characterization with obesity,insulin resistance(IR)，blood pressure (BP), blood lipid spectrum. Methods The polymorphism of the β-3-AR gene was determined in 163 patients with PCOS and 100 controls. Body mass index(BMI), waist to hip circumference ratio(WHR), BP were examined. Blood glucose,blood lipid spectrum,insulin were also measured. Insulin resistance index was calculated. Results The frequency of Arg64 allele in PCOS women was significantly higher than that of controls(0.33 vs 0.13 respectively, P<0.05). Fasting insulin, insulin resistance index, WHR in Arg64 group were significantly higher than those of Trp64 group. The frequency of Arg64 allele was positively correlated with FINS, insulin resistance index, WHR. Conclusion The Arg64 mutation in the β-3-adrenergic receptor(β-3-AR) gene might contribute to development of PCOS and IR.