Objective To investigate the effect of pre- and post-menopausal periods and menopausal-age limit on lipid metabolism in hypertensive women. Methods One hundred thirty-one hypertensive women in pre- or post-menopausal periods were collected for the determination of blood triglycerides(TG),total cholesterol(TC),high density lipoprotein cholesterol(HDL-C)and low density lipoprotein(LDL-C). The blood lipid levels were compared between hypertensive and control groups,as well as between pre- and post-menopausal subgroups,and the relationship between menopausal-age limit and blood levels lipid was analyzed. Results Hypertensive group in pre-menopausal period had significantly higher TC and LDL-C than did the control group〔TC,(5.27?0.47)vs(4.33?0.70)mmol/L;LDL-C,(3.50?0.45)vs(2.67?0.50)mmol/L respectively, all P

Objective:To study the distribution of the literature and the trend of international research of proliferative vitreoretinopathy (PVR) in 10 years from 2009 to 2018.Methods:Using Web of Science database of the American Institute of Scientific Information as the data source to collect literature on proliferative vitreoretinopathy published from 2009 to 2018 and a statistical analysis on the distribution of the publication time, nation and funding agency was made to figure out the institution, periodical, author with highest citations respectively among the collected literature.Meanwhile, the bibliometric analysis software Citespace and VOSviewer were employed to carry out the distribution analysis and cluster analysis of keywords as well as the co-citation cluster analysis.Results:A total of 905 articles related to PVR were included, mainly in English, most of which were written by American authors, and the number of literature published by Chinese authors was ranked the second.Among all the article funding institutions, the National Natural Science Foundation of China ranked NO.1 with the most funded articles.Citations increased year by year since 2009 and increased obviously since 2013.The journal with the highest citations was Investigative Ophthalmology & Visual Science, and the research institution with the highest citations was Harvard University.The statistical analysis of the citation quantity also showed that the top five authors with the highest citations were all from Massachusetts Eye and Ear, Department of Ophthalmology, Harvard Medical School.The results of cluster analysis showed that there were 41 high-frequency subject terms, and they were mainly grouped in 4 categories, which were etiology, risk factors, molecular mechanism, treatment and management of PVR.Keywords network visualization and overlay visualization both showed that etiology and risk factors were the focus of current research, while epithelial mesenchymal transition, transforming growth factor-β and fibrosis were new research fields in recent years.The results of citation cluster analysis showed there were 12 co-citation clusters. Conclusions:The literature of PVR shows an upward trend, and the researches focus on the molecular mechanism, prevention, and neuroprotection of PVR, which may be the future PVR research direction.

With the change of medical model and the transfer of health to communities and rural towns,the expanding enrollment of colleges and universities makes collegiate internship progrms more and more difficult Clinical College of Guilin Medical University explores the new methodology of teaching practice,constructs and practises the new molde of internal madichine teaching,Combines the coummunity internship programs with hospital internship programs.and was improved the quality of practice teaching

BACKGROUND:The pathogenesis of ligamentum flavum hypertrophy remains poorly understood, and the expression of transforming growth factor beta1 (TGF-β1) is increased notably. Reactive oxygen species (ROS) accumulation is associated with tissue degeneration, which may accelerate the progression of ligamentum flavum hypertrophy by upregulating TGF-β1 expression. OBJECTIVE:To clarify the effect and significance of ROS H2O2-mediated up-regulation of TGF-β1 and collagen type Ⅰ in the progress of ligamentum flavum hypertrophy. METHODS:Ligamentum flavum was removed from a case of acquired lumbar disc herniation with normal ligamentum flavum during lumbar posterior decompression surgery, and then separated and cultured in vitro to the 4-6 generations, followed by exposure to H2O2 at various concentrations (0, 50, 100, 150, 200μmol/L) for 72 hours. The mRNA and protein expression levels of TGF-β1 and collagen type Ⅰ were detected by real-time PCR and western blot assay, respectively. RESULTS AND CONCLUSION:Real-time quantitative PCR showed that the mRNA expression level of TGF-β1 was significantly increased in the 150 and 200μmol/L groups (P<0.05). The mRNA expression level of collagen type Ⅰ was significantly higher in the experimental groups than that in the control group, especially in the 200μmol/L group (P<0.05). Western blot assay revealed that the protein expression levels of TGF-β1 and collagen type Ⅰ were significantly increased in a dose-dependent manner (P<0.05). These findings indicate that H2O2 may accelerate the progression of ligamentum flavum hypertrophy by up-regulating the expression levels of TGF-β1 and collagen type Ⅰ.

Objective To explore the indication and the methodology of transcatheter closure of coronary artery fistula.Methods Altogether 17 cases for intervention,included 13 using the Cook corp coil(2 detachable and another 11 un-detachable);1 with the Pfm corp detachable coil;3 with the AGA corp Amplatzer-Duct.The other 26 cases underwent surgery. Results Intervention group,showed 3 failure cases because of selecting too small and un-detachable coil in early stage with final displacement.Fourteen cases were successful.Follow-up 3 years showed no residue shunt and other complications.All patients were successful with surgery.Conclusions Transcatheter closure of the coronary artery fistula is mild invasive,short hospitalization,safe,and effective under proper indications.

In recent years, the subthreshold micropulse laser is a kind of laser mode which is characterized by long intermittence. It achieves effective therapeutic effect while minimizes the damage to tissues. At present, it has been used to treat diabetic macular edema. Early studies suggested that the laser selectively acts on retinal pigment epithelial cells to reduce macular edema by regulating the expression of inflammatory biomarkers, growth factors, heat shock proteins and other substances. In recent years, with the development of research, more and more emphasis has been placed on the role of retinal glial cells. Müller cells are also considered as one of the target cells affected by micropulse laser, but there is no evidence of direct or indirect effects of micropulse laser on Müller cells. In the near future, it is expected that we will have more clinical evidence to confirm the target cells of the micropulse laser, which may be further confirmed by in vitro experiments through Müller cells or Müller cells co-cultured with retina pigment epithelium cells, so as to make a more detailed statement on the mechanism of it.

Objective To explore the effect of sarcoplasmic reticulum Ca2+-ATPase (SERCA2a) overexpression on neuroendocrine system in chronic heart failure beagles. Methods The cardiac contraction function of chronic heart failure beagles were assessed by echocardiography before and after SERCA2a gene transduction. Radiation immune method was used to test the serum level of neuroendocrine factors such as Angiotensin Ⅱ, atrial natriuretic peptide, endothelin-1 and TNF-α, IL-6. Results The cardiac contraction function of chronic heart failure beagles improved greatly (P<0.05), and the serum levels of neuroendocrine factors significantly reduced after SERCA2a overexpression (P<0.05). Conclusion The vicious circle of neuroendocrine system may be blocked partially by SERCA2a overexpression.

Objective To investigate the differential value of contrast-enhanced ultrasonography (CEUS) and time-intensity curves(TIC) in diagnosing testicular and epididymal mass lesions.Methods CEUS via intravenous bolus injection of SonoVue and TIC were performed for quantitative analysis of testicular and epididymal mass lesions.Forty-one patients with 42 testicular and epididymal mass lesions and 26 normal testicles were examined with CUES,the perfusion progress were recorded dynamically,which findings were compared with surgery.Results Twenty-three (54.76％) malignant masses displayed enhanced pattern as evenly enhanced,fast-in and fast-out (8.7％),evenly enhanced,fast-in and slow-out (65.2 ％ ),unevenly enhanced,fast-in and slow-out (26.1％ ).Ninteen (45.24 ％ ) benign masses revealed enhanced pattern as unevenly enhanced,fast-in and slow-out ( 10.5 ％),evenly enhanced,slow-in and slowout ( 10.5 ％ ),unevenly enhanced,slow-in and slow-out ( 36.9 ％ ),without enhancement ( 42.1％ ).There was statistical difference of peak intensity,time to peak and areas among malignant group,benign group and normal group ( P ＜ 0.05).Conclusions CEUS combined with TIC could provide differential diagnostic value for testicular and epididymal mass lesions.

OBJECTIVETo determine the optimum compatibility extraction technology of Rhei Radix et Rhizoma, Scutellariae Radix and Phellodendri Chinensis Cortex.

METHODThe drugs were combined respectively firstly, then use HPLC to determinate extraction rates of evaluating indicators including emodin, chrysophano, baicalin and berberine hydrochloride. Orthogonal experiment was used to detect the effect on concentration and volume of ethanol, times of extraction.

RESULTThe extraction rates of evaluating indicators were relatively high in combined extraction of Rhei Radix et Rhizoma with Scutellariae Radix and single extraction of Phellodendri Chinensis Cortex. The optimized combined extraction were 12 times more than the volume of 60% ethanol solution, by boiling for 3 times, each for 1.0 hour, and that of single extraction were 10 times more than the volume of 60% ethanol solution, by boiling for 3 times, each for 1.0 hour.

CONCLUSIONThe optimized compatibility extraction technology is reasonable, stable and practical.

Chemical Fractionation ; methods ; Chromatography, High Pressure Liquid ; Drugs, Chinese Herbal ; analysis ; isolation & purification ; Ethanol ; chemistry ; Phellodendron ; chemistry ; Scutellaria baicalensis ; chemistry ; Time Factors

Clinical data of 50 patients with gastric injury after blunt abdominal trauma(gastric rupture in 20 and gastric laceration in 30)were retrospectively analyzed.All the patients with gastric rupture had a satiate stomach.which was not found in the patient with gastric laceration.Forty-nine patients experienced combinant injury.The most common combinant injury of gastric laceration was liver and pancreatic injury(15/30 and 10/30),and the most frequent combinant injury of gastric rupture was spleen injury(11/20).Forty-eight patients recovered while 2 died after the treatment with gastric prosthesis.The mortality rate was 4%.Surgical operation,or prosthesis,could be the main treahnent of choice.The management of combinant injuries and complications should be imprta.