Objective To assess the association of air pollution and preterm birth in Taiyuan.Methods The preterm birth data from November 2005 to January 2007 were from Taiyuan birth surveillance system,meteorological data and air pollution data were from Shanxi Meteorological Bureau and Taiyuan Environmental Monitoring Station respectively.The case-crossover analysis and conditional Logistic regression model were used to analyze the association of air pollution and preterm birth in Taiyuan,while the meteorological factors were controlled.Results The stronger effective period of PM10 was 5-day lag and 6-day lag,of SO2 was 7-day lag and 8-days moving average.When PM10,SO2 increased 50 ?g/m3,for PM10,the OR of a 5-day lag and a 6-day lag were 1.169(95%CI:1.058～1.292),1.235(95%CI:1.115～1.367)respectively,for SO2,the OR of a 7-day lag and a 8-days moving average were 1.346(95%CI:1.122～1.616),2.203(95%CI:1.517～3.201)respectively.In sensitivity analysis,the lagged effects of PM10 and the cumulative effects of SO2 were steady.Conclusion The air pollution in Taiyuan may produce an acute effect on preterm birth.

According to their polypeptide length and number of d is ulphide bonds, disintegrins are divided into five groups, including short-sized disintegrins, medium-sized disintegrins, long-sized disintegrins, dimeric dis integrins and disintegrin-like molecules released from the disintegrin-like do mains of P-III snake venom metalloproteinases. The integrin-inhibitory activit y of disintegrins, to inhibit platelet aggregating, cell attaching and angiogene sis, depends on the active tripeptide RGD, the appropriate pairing of cysteine r esidues, the amino acids adjacent to the RGD motif within the integrin-binding loop and the C-terminus of the disintegrin polypeptidesl as well.

Objective To investigate the clinical treatment of nonpenetrated cornea trauma caused by crops. Methods Clinical data of 128 cases of nonpenetrated cornea trauma caused by crops were retrospectively analyzed. According to the interval time between occurrence of trauma and clinic visiting, the patients were divided into 3 groups:group A (33 cases,<24 h), group B (72 cases, 24 h≤interval time<1 week) and group C (23 cases, ≥ 1 week). The therapeutic effects and prognosis were analyzed. Results There was statistical difference in the incidence of corneal ulcer among group A, group B and group C: 6.1% (2/33), 62.5% (45/72) and 100.0% (23/23), χ2= 52.32, P<0.01. In group B, 12 cases were treated with conjunctival flap covering, 2 cases received keratoplasty and 2 cases undertook enucleation. In group C, 10 cases were treated with conjunctival flap covering, 4 cases received keratoplasty and 2 patients undertook enucleation finally. All the other patients were cured with local debridement and medical treatment. Conclusions Patients with nonpenetrated cornea trauma caused by crops may develop infectious keratitis, and prompt and proper treatment can avoid the secondary infection and improve the outcome. Local debridement in combination with iodophors disinfection can prevent the incidence of infectious keratitis. Conjunctival flap covering is an effective technique in the treatment of corneal ulcer caused by nonpenetrated cornea trauma.

Objective To investigate the interactions and effects of Helicobacter pylori (H.pylori) and non steroidal anti inflammatory drugs (NSAID) on the proliferation of gastric epithelial cells in vitro. Methods After co culturing of gastric cancer cell line AGS cells with H.pylori and/or NSAID (indomethacin and aspirin) for 48 hours, the cell proliferation and proliferating cell nuclear antigen (PCNA) were examined by MTT assay and Western blot. Results cagA positive H.pylori strain NCTC11637, but not cagA negative H.pylori strain NCTC12908, had the effect of enhancing gastric epithelium cell proliferation. However, the effect of proliferation was dependent on the density of H.pylori . It was demonstrated that low density (range from 3.2?10 4 CFU/ml to 4?10 6 CFU/ml) of bacteria suspensions resulted in proliferative effect, while high density (more than 2?10 7 CFU/ml) resulted in inhibition. Besides, indomethacin and aspirin inhibited cell proliferation in a dose dependent manner. Moreover, when AGS cells were incubated with cagA positive H.pylori and NSAID, inhibition rather than proliferation was observed. cagA positive H.pylori strains up regulated the expression of PCNA while indomethacin and aspirin down regulated the level of PCNA expression. Meanwhile, the expression of PCNA was also reduced significantly when AGS was co cultured with H.pylori and NSAID. Conclusions The results indicated that gastric epithelium cell proliferation was associated with different H.pylori strains and its density. cagA positive H.pylori strain is prone to increase cell proliferation, but cagA negative H.pylori strain has no such effect. NSAID can inhibit gastric epithelial cell proliferation and reverse such effect caused by H.pylori.

BACKGROUND: Revascularization is a challenge for the tissue-engineered bone carrying cells after implanted into human body. Previous studies have found that tanshinol can improve the functions of endothelial progenitor cells and exert vascular protective effects.OBJECTIVE: To prepare the β-calcium phosphate (β-TCP) scaffold with tanshinol coating, and to observe its cytocompatibility.METHODS: The β-TCP scaffolds coated with 10-7, 10-6 and 10-5 mol of tanshinol were constructed by negative pressure absorption method. The distribution of tanshinol coating on the scaffold was observed using scanning electron microscopy,and the inner ingredients were analyzed by infrared spectrum. Human endothelial progenitor cells (hEPCs) were cultured in the extracts of β-TCP and β-TCP scaffolds with 10-7, 10-6, 10-5 mol of tanshinol coatings, respectively. The cell proliferation was detected at 2, 4, 6, 8 and 10 days of culture; the levels of nitric oxide and vascular endothelial growth factor in the supernatants were detected at 1, 7 and 14 days of culture; the lumen formation on the matrigel was observed after 14-day culture. hEPCs were respectively seeded onto the β-TCP and β-TCP scaffolds with different dosages of tanshinol coating,and then the cell growth was observed under scanning electron microscope at 7 days.RESULTS AND CONCLUSION: The tanshinol coating evenly distributed on the inner surface of the pores, and its crystalline structure became dense with dosage increasing. Infrared spectrum analysis revealed no changes in the characteristic absorption peak of tanshinol and TCP in the scaffold. The β-TCP scaffolds with tanshinol coating could promote the proliferation of hEPCs, especially the scaffolds with 10-6 and 10-5 mol tanshinol coating. Compared with the β-TCP scaffold, the scaffolds with 10-6 and 10-5 mol tanshinol coating significantly upregulated the nitric oxide level at 14 days of culture, and significantly increased the level of vascular endothelial growth factor at 7 and 14 days of culture (P <0.05). Although it could be found in all β-TCP scaffolds with tanshinol coating, the lumen formation was the maturest in the scaffold with 10-5 mol tanshinol coating. These results suggest the β-TCP scaffolds with tanshinol coating can promote the proliferation and endothelial differentiation of hEPCs, and hold a good cytocompatibility.

Objective To know the postnatal depression and anxiety state and its related factors of parturients from migrant worker family,and then reference to certain nursing counter-measures. Methods Sampled 73 parturients by spontaneous labor from migrant worker family and 45 local matched parturients,interviewed them by SAS and SDS to know their postnatal depression and anxiety state,and then analyzed the datum between them. Results The level of postnatal depression and anxiety in parturients from migrant worker family was significant higher than that in local parturienta.Different level of depression and anxiety was existed in different parturients from migrant worker family by different educational attainments,different family type and different financial state. Conclusions The state of postnatal depression and anxiety in parturients from migrant worker family is severely,related health education should be strengthened,associated social secu-rity and support system should be established to help them release their unhealthy emotion.

Objective To investigate the epidemiology and relevant risk factors of invasive fungal infection (IFI) in hospital old patients for non-respiratory tract. Methods Seventy-eight patients of IFI in non-respiratory tract were enrolled in this investigation. The incidence and risk factors of IFI were analyzed by prospective case-control study. Results In 78 old patients, 84 strains were isolated from different parts, and the most was Candida spp 82 strains (97.62%,82/84), followed by Candida albicans 55 strains (67.07%,55/82), Candida glabrata 13 strains ( 15.85%, 13/82), Candida krusei 6 strains (7.32%, 6/82), Candida tropicalis 4 strains (4.88% ,4/82), Candida parapsilosis 3 strains (3.66% ,3/82), Candida lusitaniae 1 strain ( 1.22%, 1/82). Aspergillus 2 strains (2.38%,2/84). Multivariate Logistic regression analysis showed that age, pathogen detection time, underlaying disease,glucocorticoids, immunosuppressants were the risk factors for IFI in non-respiratory tract. Conclusions Candida albicans is the main pathogens of Candida infections in old patients. To efficiently control the risk factors should be emphasized in old patients, including early diagnosis and treatment underlying diseases, appropriate use drugs, right to shorten hospital stay.

Objective To investigate the etiology features and relevant risk factors of non-albicans candida infections in hospital. Methods 256 patients of non-albicans candida infections admitted in the second hospital of shanxi medical university from April 2006 to March 2008 were enrolled in this investigation, and a prospective case-control study was executed on 256 cases of non-albicans candida infections and 1220 cases of non-fungal infections. The incidence and risk factors of non-albicans candida infections were analyzed by statistical software SPSS13.0. Results Candida glabrata was the most common reason of non - albicans candida infections (38. 28% ) , followed by candida krusei (37. 11% ), candida parapsilosis ( 12. 50% ), candida tropicalis (9. 77% ), candida lusitaniae (2. 34% ). Univariate analysis and multivariate logistic regression analysis showed that aging, length of stay, underlying disease, losing albumin, using prophylaxis antifungal drugs, using broad spectrum antibiotics, invasive examination and treatment ( such as total parenteral nutrition ( TPN ), invasive procedures, central venous catheters, hemodialysis and mechanical ventilation,et al. ) were the independent risk factors for non-albicans candida infections. Conclusions Non-albicans candida was the main of fungal infections in patients. To efficiently control the disease, it will be helpful by early diagnosis and treatment underlying diseases and commodities and using appropriate tools of examine and treatment methods.

Objective To summary the therapy outcome of retroperitoneal laparoscopic treatment on upper ureteral calculi.Methods The retroperitoneal laparoscopic treatment were performed to all cases from Jun.2011 to Jun.2012 in our hospital.All cases were treated with 3-hold method (two 10 mm and one 5 mm ports).The retroperitoneal space was made by a combination of blunt and balloon dissection,and the space was maintained with CO2 Ureteral longitudinal incision was made to remove the stones,and double J catheter was served as stent drainage.Absorbable suture was used to suture ureteral incision.Results A retroperitoneal approach was performed in 12 patients,and another patient was conducted the open surgery because the stone cower in the kidney calices.Operative periods ranged from 55 to 132 min (average was 85 min).There were no significant postoperative complications.Conclusion It is a minimally invasive and effective approach in the therapy of upper urerteral calculi with laparoscopic.

Objective: To explore the intervention techniques of group counseling leader ("group leader") during group counseling process. Methods: The analysis material of intervention techniques was 11 sessions in two unstructured counseling groups. The analysis unit was every dialogue paragraph in the leader'soral response in the group counseling process. And the leaders' intervention techniques emerging in the video and verbatim transcription were coded according to adapted coding manual. Results: Totally 48 types of techniques were identified, that were grouped into 4 categories, including basic communication and reaction technique, exploring and influencing technique, facilitating technique, action and intervention technique. From the perspective of the single group counseling activity, techniques of basic communication and reaction were used more in the early and middle stage of an activity. Techniques of exploring and influencing and techniques of action and intervention were used more in the middle and later stage. And facilitating techniques were more used in the early and later stage. From the perspective of the whole grouping counseling process, techniques of basic communication and reaction and techniques of exploring and influencing were two primary types of intervention techniques. Frequency of using exploring and influencing techniques reached its peak in the intermediate stage of the group counseling process. The use of facilitating technology was gradually reduced. Conclusions: Techniques of basic communication and reaction are basic intervention techniques in group counseling, while techniques of exploring and influencing and techniques of action and intervention could be used on the basis of the development of the group. And facilitating techniques could facilitate to open the group and deepen the achievement of group counseling.