This article introduced a method to extrct and purify the DNA of Cryptococcus. Protoplasts were obtained after digested by snail enzyme. We washed them with SE liquid again and again to get rid of the capsular polysaccharides and split them with thermal treatment of 55 degrees centigrade. Then, satisfactory results of the extrction and the purification were achieved. From one wet gram of the yeast, more than 600 ?g DNA can be acquired. The DNA gained is especially suited for the determination of G+C mol% content by use of the T_m method.

Mantle cell lymphoma (MCL) is a rare subtype of non-Hodgkin lymphoma.The MCL is classified into two subtypes, some patients have a chronic/indolent course and the others have a more aggressive course and short survival.In recent years, some novel therapeutic target agents for MCL have reports.These target agents achieve efficacy comparable to conventional chemotherapy in relapsed/refractory MCL.Therapeutic targents for MCL include the surface antigens of B-cell lymphoma, B-cell receptor signaling, DNA damage response pathway and the cellular microenviroment.This review focused on the recent research progress of target agents in the treatment of MCL.

0.05. CONCLUSION:Kaixin Powder could enhance VD rats' memory; while Tacrine could enhance VD rats' learning; Kaixin Powder could enhance VD rats' learning and memory as well as Tacrine. Tacrine could down-regulate AChE mRNA expression in hippocampus of VD rats,further studies are needed to elucidate the effects of Kaixin Powder on memory performance in VD rats.

Objective To explore the TCM pathogenesis based on the analyses of TCM pattern elements. Methods TCM pattern elements of 84 patients with middle-late pancreatic cancer to analyze the TCM disease location and characteristics by their frequency and the contribution scores. Results A total of 84 patients were included, TCM disease location with the top 10 rank of the contribution scores were spleen, stomach, liver, gallbladder, kidney, intestine, lung, heart, spleen, stomach, while they were significantly higher than others (P<0.05). And the TCM pattern elements with the top 5 rank of the contribution scores were qi deficiency, dampness, heat, blood stasis, toxin, while they were significantly higher than others (P<0.01). Conclusions TCM disease locations of middle-late pancreatic cancer were mainly in spleen and stomach, and TCM pattern element were excessive patterns with qi deficiency, deficiency patterns with dampness, heat, blood stasis and toxin.

Objective To evaluate the effects ofperioperative enteral nutrition on host immunologic function in elderly patients with gastrointestinal tract malignant tumor. Methods Eighty elderly patients with gastrointestinal tract malignant tumor were divided into two groups by random digits table with 40 cases each: experimental group was given enteral nutrition and control group was given parenteral nutrition.Peripheral blood samples were collected at the fifth and the first day before operation, and the fust and the seventh day after operation to examine the NK cell,T-lymphocyte subsets (CD3, CD4,CD8) and immunoglobulin (lgG, lg A, IgM ). Result There were significantly increased in NK cell [(40.1 ± 4.9)%], CD3[(69.40 ±7.10)%], CD4[(39.20 ± 5.83)%], IgA[(2.94 ±0.56) g/L] and IgM[(1.34 ±0.72) g/L] of experimental group compared with those of control group [(26.4 ± 4.9 )%, ( 61 .40 ± 8.32)%, ( 29.20 ± 5.65 )%, ( 1.72 ±0.76) g/L, (0.97 ± 0.89 ) g/L] (P ＜ 0.05 ). Conclusion The application of enteral nutrition in perioperative period can improve the immunologic function of elderly patients with gastrointestinal tract malignant tumor,reduce surgical risk and postoperative complications.

Using modem network and computer technology and characteristic digital resources, E - reading room has changed utiliza-tion modes of library information resources. The paper introduces the formation, current status and developing strategies of E - reading room open information service, and points out that this kind of service has become new model of library service in information era.

Objective To discuss the microcosmic elements and syndromes distribution of H pylori-related gastritis. [Method] Choose 100 cases, under gastroscope report, make statistics of frequency of disease nature elements and location elements, combine the nature and location for common syndromes. [Result] The H pylori-related gastritis has main disease location elements in stomach, esp. the sinuses ventriculi;the disease nature elements mainly concern hot, wet, sputum stasis, deficiency and blood stasis; the common syndromes: hot stomach, sputum and stasis blocking stomach, wet-hot accumulated in stomach, deficient stomach, wet stranding stomach and stasis blocking gastric col aterals. [Conclusion] Over the inconsistent syndromes in clinical H pylori-related gastritis, the microcosmic elements differentiation can further standardize syndromes and guide the treatment.

Atrial fibrillation (AF) is one of the most common arrhythmias in clinical practice.It often causes serious thromboembolic complications,which brings great harm to human health.The incidence and morbidity rates of AF are increasing year by year.The research on the pathogenesis of AF has been focused on the aspects of electrical remodeling and structural remodeling.Atrial electrical remodeling and structural remodeling are the main mechanism of AF for the maintenance and recurrence.The intervention of atrial remodeling is helpful to the treatment of AF.It is found that the rennin-angiotensin aldosterone system (RAAS) plays an important role in atrial remodeling.RAAS blockers can significantly reduce the onset of AF by inhibiting the activation of the renin axis,which can block the activation of RAAS and improve the atrial remodeling.This may provide a new choice for the treatment of AF.This article reviews the research progress of RAAS blocking agent in the prevention and treatment of AF.

Objective]The paper summarizes academic thought of Professor ZHAO Zhiqiang syndrome differentiation and treatment of adenomatous polyp of colon. [Method]By learning from ZHAO Zhiqiang and studying the medical record of adenomatous polyp of colon,sum up professor ZHAO Zhiqiang′ s experiences from etiology and pathogenesis, differential treatment, administered medical herbs and prescription ,and with one case for detailed explanation. [Results] Professor ZHAO thinks that the pathogeny raletes to exopathy, diet, emotion, weakness of the spleen and stomach, physique, smoke poison,influence of other diseases,the key of pathogenesis is phlegm, blood stasis and toxin stagnation, accumulation of intestine, Qi is out of adjustment, the conduction loss of us, He believes that the syndrome diagnosis of the disease is different from others, the syndrome diagnosis should include disease syndrome elements, syndrome elements of disease location and morbid state, disease syndrome elements should need the help of the modern medical endoscope and pathology, and ill treatment to detoxify the anti-cancer variable as the core, supplemented with Ruanjian Sanjie,coordinating zang and fu, modification according to symptoms.[Conclusion] Professor ZHAO's treatment of adenomatous polyp of colon has rich experience and prominent features, it is worth clinical promotion.

Objective To study the expression of high molecular weight cytokeratin (CK34βE12) in breast carcinoma and the relationship between the high molecular weight cytokeratin expression and patient's prognosis. Methods We detected the high molecular weight cytokeratin expression in 100 patient's of breast cancer tissues and paracancerous breast tissue in 30 cases immunohistochemically. The relationship between the expression of high molecular weight cytokeratin in breast cancer tissues and age, menstrual status, estrogen receptor expression, progesterone receptor expression, histology grading, TNM staging, pathology type, and patient survival was analyzed. Results (1) There was no significant correlation between high molecular weight cytokeratin expression of breast -cancer tissues and age, menstrual status, estrogen receptor expression, progesterone receptor expression (all P>0.05). But the correlation was statistically significant between that expression and histology grading, TNM staging, pathology category (all P<0.05). (2) By univariate analysis, the 5-year disease-free survival rate in patients with negative expression was lower than those with positive expression(P<0.05). By multivariate analysis, the expression of high molecular weight cytokeratin was unfavorably related with tumor free survival. Conclusion The expression of high molecular weight cytokeratin in breast carcinoma tissues was correlated with biologic malignancies of breast cancer, the negative high molecular weight cytokeratin expression of breast cancer tissues predicts poor prognosis.