Objective To evaluate the effect of total mesorectal excision with single stapling technique for low positioned rectal cancer.Methods Based on total mesorectal excision,56 cases of low positioned rectal cancer underwent anal preserving operation with single stapling technique and purse-string forceps.The 2 years follow-up result was retrospectively analyzed on radical resection effect,defecating function,complication rate and recurrent rate.Results All the resection margins were clear and had no intraoperative death,anastomotic leakage or occurrence of incontinence.Anastomotic stricture rate was 4%(2/56) and local recurrent rate was 5%(3/56).The patients were satisfied with anal function.Conclusion Based on total mesorectal excision,anal preserving operation for low positioned rectal cancers using single stapling technique and purse-string forceps can improve the postoperative quality of life,that is safe,feasible,practical and economical.

Objective To investigate the effect of narrow-band UVB combined with tacrolimus ointment in the treatment of vitiligo,and its influence on IL-17 and IL-22 in peripheral blood.Methods 100 patients with vitiligo were selected as study subjects,and they were divided into the control group and observation group according to the random number table method,50 cases in each group.The control group was given 0.1% tacrolimus ointment,the observation group was treated with narrow-band UVB combined with 0.1% tacrolimus ointment.After 6 months of treatment,the clinical treatment effect of the two groups was observed.The levels of serum IL-17 and IL-22 were detected by ELISA,the differences between before and after treatment were compared between the two groups.Results The cure rate,effective rate of the observation group were 74.0%,96.0%,respectively,which were significantly higher than 62.0%,84.0% of the control group,the differences were statistically significant(x2=3.125,2.897,all P<0.05).The levels of IL-17 and IL-22 in the two groups before treatment had no statistically significant differences(all P>0.05).After treatment,the levels of IL-17 and IL-22 in the two groups significantly decreased(t=3.454,3.032,all P<0.05),which in the observation group were lower thanthose in the control group(t=3.867,2.665,all P<0.05).Conclusion Narrow-spectrum UVB combined with tacrolimus ointment has good clinical effect on vitiligo,which can effectively improve the local immune response.

Objective To investigate the effect of compound glycyrrhizin combined with fexofenadine hydrochloride in the treatment of chronic urticaria and its effect on plasma histamine.Methods 100 patients with chronic urticaria were selected as study objects,and they were divided into the control group(50 cases) and observation group(50 cases) according to the random number table method.The control group was treated with fexofenadine hydrochloride,while the observation group was treated with compound glycyrrhizin on the basis of the control group.The clinical effect and plasma histamine level were compared between the two groups.Results The effective rate of the observation group was 96.0%,which was significantly higher than 84.0% of the control group(χ2=7.951,P<0.05).After treatment,the plasma histamine content of the observation group was (2.38±0.26)ng/mL,which was lower than (3.10±0.29)ng/mL of the control group,and the difference between the two groups was statistically significant(t=6.084,P<0.05).Conclusion The compound glycyrrhizin combined with fexofenadine hydrochloride in the treatment of chronic urticaria can reduce the symptoms of the patients,reduce the number and size of the patients and effectively control the clinical symptoms of the patients.And it can effectively reduce the level of plasma histamine in patients with good clinical effect and application value,it is worth to be promoted in clinical.

Using modem network and computer technology and characteristic digital resources, E - reading room has changed utiliza-tion modes of library information resources. The paper introduces the formation, current status and developing strategies of E - reading room open information service, and points out that this kind of service has become new model of library service in information era.

Objective:To discuss the anti-rheumatoid arthritis activity and mechanism of hospital preparation Erteng Tongbi gran-ule ( ETTB) . Methods:The collagen-induced arthritis ( CIA) animal model in DBA/1 mice was established and treated with high, medium and low dosage of ETTB. Blank control, model control and methotrexate group were also established. The influences of ETTB on BMI, arthritis index, ankle joint destruction and expression of serum cytokine TNF-α and IL-6 in CIA mice were observed. Re-sults:Compared with the control group, ETTB at high dose (6g?kg-1) could significantly decrease the arthritis index of CIA mice (P<0. 05), and the joint damage on the 14th day was also significantly improved. ELISA results showed that ETTB at high dose could significantly inhibit the secretion of cytokine TNF-αand IL-6 when compared with the model group (P<0. 01). Conclusion:Hospital preparation ETTB has anti-rheumatoid arthritis activity, and its mechanism may be related with the inhibition of cytokine secretion.

Objective To investigate the effect and mechanism of ultrashortwave(USW)diathermy and rotating magnetic(RM)field therapy on the brain following transient focal cerebral ischemia and reperfusion in rats. Methods Fifty-two Wistar rats were randomly divided into a sham operation group, an ischemia and a reperfusion control group, a USW treatment group and a RM treatment group. Focal cerebral ischemia and reperfusion were induced by intraluminal filament occlusion of the middle cerebral artery. Each brain was removed at 24 h after the reperfusion and water content, cerebral infarct volume and histological expression of Bcl-2 and Bax proteins were observed. Results When USW treatment was started at 18 h after reperfusion, decreased infarct volume and water content were observed, but RM treatment did not show this relationship. Both USW and RM were associated with increased expression of Bcl-2 protein and decreased the expression of Bax protein. Conclusions USW treatment is beneficial in focal cerebral ischemia. RM′s effect is uncertain. Any effect is through reducing apoptosis.

Objective To discuss the microcosmic elements and syndromes distribution of H pylori-related gastritis. [Method] Choose 100 cases, under gastroscope report, make statistics of frequency of disease nature elements and location elements, combine the nature and location for common syndromes. [Result] The H pylori-related gastritis has main disease location elements in stomach, esp. the sinuses ventriculi;the disease nature elements mainly concern hot, wet, sputum stasis, deficiency and blood stasis; the common syndromes: hot stomach, sputum and stasis blocking stomach, wet-hot accumulated in stomach, deficient stomach, wet stranding stomach and stasis blocking gastric col aterals. [Conclusion] Over the inconsistent syndromes in clinical H pylori-related gastritis, the microcosmic elements differentiation can further standardize syndromes and guide the treatment.

Osteoporosis is a systemic metabolic degenerative skeletal disease, characterized by reduced bone mass and disrupted bone microarchitecture, leading to enhanced bone fragility, decreased bone strength and increased susceptibility to frac?tures. Its occurrence and development is an extremely complex biological process, which is a multi factor, multi gene and multi stage experience. Osteoporosis is the fourth chronic disease in our country, which mainly affects postmenopausal women and the el?derly. Bone mass is jointly maintained by the action of bone resorption by osteoclasts and bone formation by osteoblasts. If the im?balance between the two occurs, resulting in reduced bone formation and increased bone resorption, bone homeostasis is changed to cause osteoporosis. Traditional treatment for osteoporosis are drugs which just to depress bone resorption (such as bisphospho?nates and estrogen), or just to promote bone formation (such as PTH and statins), or just to supply the source of calcium. This sin?gle point of local treatment is clearly difficult to play a long?term part in complex lesions of osteoporosis. In recent years, research?ers have studied the molecular signaling pathways related to osteoporosis. The current studies reveal that signaling pathways in os?teoporosis include MAPK, PI3K/Akt, Wnt/β?catenin, RANKL/RANK/OPG, Hedgehog and Notch signaling pathways. These signal?ing pathways play an important role in the molecular pathogenesis of osteoporosis. In addition, there are GH/IGF?1, PPAR?γ, PTH signaling pathways and so on. Taking these signaling pathways as targeting points is a new strategy for the development of new anti osteoporosis drugs or the prevention of osteoporosis. But there are cross?talks and/or interactions between these signaling pathways related to osteoporosis and the pathogenesis of osteoporosiswhich make its therapy more difficult. This paper reviews the research progress of signaling pathway in osteoporosis in order to explore the molecular mechanism of its occurrence. Thus these signaling pathways can be taken as new targets to develop safer and more effective anti?osteoporosis drugs.

BACKGROUND:Human leukocyte antigen F-associated transcription factor 10 (FAT10) is highly expressed in many tumor cel s like colon cancer cel s, but its relationship with esophageal cancer is less reported. OBJECTIVE:To investigate the effects of siRNA interference technique on the invasion, apoptosis and the characteristics of EC9706 cel s, a human esophageal cancer cel line. METHODS:siRNA sequence was designed and synthesized according to the FAT10 mRNA encoding sequence, and the EC9706 cel s were transiently transfected. EC9706 cel s were divided into three groups:siRNA FAT10 group, negative control group, and blank control group. The expression levels of bcl-2 and FAT10 were detected by RT-PCR and western blot assay, respectively. Cel counting kit-8 assay was used to measure the proliferation of cel s in vitro. Flow cytometry was used to observe the changes of cel cycle, cel apoptosis and the expression of CD44+CD133+. TUNEL staining was used to detect the apoptosis of the cel s. Cel invasion in vitro was detected by Transwel invasion assay. RESULTS AND CONCLUSION:RT-PCR and western blot findings showed that compared with the negative control group and blank control group, the expression levels of bcl-2 and FAT10 mRNA and protein were significantly decreased in the siRNA FAT10 group (P<0.05);the percentage of CD44+CD133+cel s was decreased significantly (P<0.05);and significantly increased apoptosis rate, and decreased cel proliferation and invasion were also found in the siRNA FAT10 group (P<0.05). In conclusion, the specific silencing of FAT10 gene can reduce the invasion of esophageal cancer cel s, inhibit cel proliferation, reduce bcl-2 expression, and increase the apoptosis rate. Meanwhile, the proportion of CD44+CD133+cel s is decreased.

Colorectal cancer is one of the most common malignant tumors. The recent successful development of targeted therapy has brought new hope for patients with colorectal cancer. Two monoclonal antibodies, bevacizumab and cetuximab, that block vascular endothelial growth factor and epithelial growth factor receptor are available widely in clinical practice. This clinical review focuses on the role of targeted therapy in the management of colorectal cancer.