Using modem network and computer technology and characteristic digital resources, E - reading room has changed utiliza-tion modes of library information resources. The paper introduces the formation, current status and developing strategies of E - reading room open information service, and points out that this kind of service has become new model of library service in information era.

Objective To study the cost-benefit analysis of magnetic resonance cholangiopancreatography (MRCP) before laparoscopic cholecystectomy (LC) in cholecystolithiasis patients unsuspected choledocholithiasis.Methods The clinical data of 960 cholecystolithiasis patients unsuspected choledocholithiasis were retrospectively analyzed.The patients were divided into MRCP group (665 cases) and non-MRCP group (295 cases) according to the application of MRCP or not before LC.The total hospitalization expense,missing diagnosis rate of postoperative choledocholithiasis,secondary admission rate,hospitalization time and intraoperative bile duct injury between the 2 groups were compared.Results There were no statistical differences in total hospitalization expense and hospitalization time between the 2 groups (P ＞ 0.05).The missing diagnosis rate of postoperative choledocholithiasis and secondary admission rate in MRCP group were significantly lower than those in non-MRCP group [0.15％ (1/665) and 0 vs.3.73％ (11/295) and 3.73％ (11/295)],there were statistical differences (P ＜ 0.05).There was no intraoperative biliary tract injury in the 2 groups.Conclusions In terms of cost-benefit analysis,routine MRCP before LC for cholecystolithiasis patients unsuspected choledocholithiasis is benefiting.Routine MRCP before LC could effectively reduce the missing diagnosis of the postoperative choledocholithiasis and the secondary admission rate without increasing the total hospitalization expense of patients.

It is vital to make an individual plan for each patient with obstructive sleep apnea-hypopnea syndrome (OSAHS) according to the obstruction sites. The high resolution anatomical information of upper airway and soft tissue can be obtained, especially by MRI and CT scans. Dynamic and state-dependent imaging techniques are beneficial to study stereo changes of anatomy and morphology of upper airway in quiet breathing, sleeping or airway closure. Although dynamic imaging examination has value in diagnosis and treatment of OSAHS, there has no uniform position diagnosis standard. This article reviews the history of dynamic imaging study on OSAHS, the advantages and disadvantages of various imaging technologies and prospects of imaging position diagnosis.
Humans ; Magnetic Resonance Imaging ; Respiratory System ; diagnostic imaging ; pathology ; Sleep Apnea, Obstructive ; diagnosis ; diagnostic imaging ; Tomography, X-Ray Computed

Early-onset scoliosis (EOS) orthopedic treatment is a worldwide problem in recent years.With the growing rod technique clinical application and development of innovative,it has opened up a new way of orthotics for scoliosis.This paper reviews the development process of growing rods in treatment of early onset scoliosis.

[Objective] To measure the nitrogen content in Agrocybe cylindracea R.Maire,compare the advantages of Kai's Nitrogen-testing method and Spectrophotometric method.[Method]Take Kai's Nitrogen-testing method and Spectrophotometric method.[Result]The two methods were almost the same in the measurement of nitrogen,i.e.30.70mg/g,which had no marked difference by t test.[Conclusion]Spectrophotometric method is simple and convenient for operation,quickly and correctly,and can be used for measurement of total nitrogen.

BACKGROUND:Along with the medicine development.the prevention and treatment of the atrophy of denervated skeletal muscle have made a remarkable progress,but the clinical curative effect is still not satisfied OBJECTIVE:To summarize the current status of the prevention and treatment methods of denervated skeletal muscle atrophy,and to find a more effective method for the prevention and treatment of the denervated of skeletal muscle atrophy METHODS:Using"denervation,muscle atrophy,treatment"in English for the search term,Medline database from January 1998 to January 2008 was retrieved.Using"denervation,muscle atrophy,treatment:in Chinese for the search term,the Chinese Journal Full-text Database from January 1998 to January 2008,Wanfang Database from January 1998 to January 2008,and Journal of Clinical Rehabilitation and Tissue Engineering Research from January 1 998 to January 2008 were searched.Literature was limited to English and Chinese languages.The muscular endurance and contractile force,muscle wet weight of denervated nerve,and skeletal muscle repair served as the evaluation index The studies on the micro-surgical procedures,physical therapy,biological and chemical therapies,gene therapy for the atrophy of denervated skeletal muscle were included.Except the above methods,other therapies to the denervated skeletal muscle atrophy were excluded.RESULTS AND CONCLUSION:After peripheral nerve damage.the skeletal muscle is denervated and then inevitably atrophy occurs Therefore,the exploration of prevention and treatment of denervated skeletal muscle atrophy has attracted the interests from many domestic and foreign scholars.will certainly become the important task and the research hot spot in the 21st century in the peripheral nerve domain The microsurgery surgery,physiotherapy,biology and chemotherapeutics,gene therapy are the main effective prevention methods for the atrophy of denervated skeletal muscle.At present,this domain.s research already presents the multiple perspectives and various tendencies.The prevention and treatment of denervated skeletal muscle atrophy has already given the pointed management,but in the improvement microcycle,but much works need to carry on in fields of improving microcirculation,preventing cell apoptosis,suppressing the collagen excessive growth,as well as adjusting myogenic regulatory factors expression using gene therapy method in the gene level,but also had the.Along with the tissue engineering,cell culture science,molecular biology,genetic engineering and so on,an unceasing development will be obtained regarding the prevention and treatment of denervated skeletal muscle atrophy.

Objective To explore the dissociation, purity and the culture method of spinal motoneurons in vitro and to find out an effective way to identify spinal motoneurons.Methods Spinal motoneurons were isolated from spinal cord of embryonic rat. Spinal tissues were digested and then the motoneurons through 6.8% metrizamide density gradient centrifugation, were obtained by collecting the top layer of metrizamide cushion. After the glia adhered to the plate wall, the enriched motoneurons were collected and plated in 24-well plates at a density of 4?10~5/ml. Cytosine arabinoside was added to the culture system after 24 hours in order to inhibit the outgrowth of glia. L-15 serum medium was used for the first 48 hours and then changed by serum-free medium. Later, neurons were cultured with 50% exchanged medium every two days. Spinal motoneurons were identified by immunostaining with polyclonal antibody to choline acetyltransferase (ChAT) according to ABC method. Laser scanning confocal microscope was used to observe its structure.Results Spinal motoneurons were immunoreactive to the antibody against ChAT. The present experiment revealed that ChAT-positive cells more than 85% might live about seven to nine days.Conclusions This study suggests a method of spinal motoneuron culture successful. Spinal motoneurons could be specially labeled with ChAT antibody.

Objective To assess the efficacy and safety of transurethral bipolar plasmakinetic resection of the prostate(TUPKRP) for the treatment of benign prostatic hyperplasia(BPH).Methods The operation was completed using a bipolar plasmakinetic resection system(CIRCON ACMI).Three trenches were created at 5,7,and 12 o'clock position,respectively,to divide the middle,left,and right lobes of the prostate gland for resection.The bladder neck was widened in overlapping vaporizing swathes to create a wide-necked funnel down to the level of the surgical capsule.The apical tissue was resected to the anterior border of the seminal colliculus.With a F_(20) or F_(22) three-cavity catheter indwelling for 3~5 d,normal saline irrigation was used for 1~2 d postoperatively.Results The duration of the procedure was 20~180 min(53.2?28.7 min),and resected tissue weighed 8~130 g(23.5?21.6 g).The intraoperative blood loss was 10~300 ml(65.4?46.8 ml) and no blood transfusion was required.No perforation of the prostatic capsule or transurethral resection syndrome occurred.The peak urinary flow rate(Qmax) increased from 8.4?1.9 ml/s preoperatively to 18.4?1.8 ml/s 1 month postoperatively(q=55.498,P﹤0.05).The residual urine(RU) decreased from 80.8?59.7 ml preoperatively to 19.5?10.0 ml postoperatively(q=17.287,P﹤0.05). The international prostate symptom score(IPSS) decreased from 25.7?5.5 preoperatively to 5.7?2.4 postoperatively(q=52.969,P﹤0.05).The scores of quality of life(QOL) decreased from 4.4?1.0 preoperatively to 1.1?0.2 postoperatively(q=42.146,P﹤0.05). Conclusions Transurethral bipolar plasmakinetic resection of the prostate for the treatment of benign prostatic hyperplasia is effective and safe,with few complications.

Objective To investigate the effects of Leptin on the base of anthropometry,densitometry,and biochemistry and reveal the role of Leptin in bone metabolism in postmenopausal women with osteoporosis (OP).Methods From January 2008 to January 2014,data of 82 postmenopausal females with or without OP were analyzed.They were randomized into two groups:OP (n=40) and conditional control group (CON) (n=42).The expression of Leptin receptors in osteoblasts was observed by immunohistochemistry.1 × 10 ng/ml,1 × 102 ng/ml,1 × 103 ng/ml,1× 104 ng/ml of Leptin were added respectively.Then the proliferation,differentiation and mineralization of osteoblasts were measure at 24 h,48 h and 96 h.The gene expressions of bone marrow mesenchymal stem cells (BMSCs) derived osteoblasts and adipocytes treated with Leptin were examined by quantitative analysis of Real time-PCR.Results The expression of Leptin receptors in osteoblasts was observed on plasma membranes and cytoplasm in the normal group and osteoporosis group.In osteoporosis group,different concentrations of Leptin enhanced cell proliferation,cell differentiation and cell mineralization.The growth rate of MTT and the concentration of ALP in the serum of were significantly increased with the effects of 1×10 ng/ml,1×102 ng/ml and 1×103 ng/ml of Leptin,and the action of 1×102 ng/ml Leptin was the most powerful with time dependence.Within 96 hours,the growth rate of osteoblasts increased gradually,and the concentration of ALP increased gradually in 3 weeks.The expression of RANKL/OPG in bone marrow BMSCs derived osteoblasts treated with Leptin were significantly increased.Conclusion Leptin receptor is present in osteoblasts,and Leptin affects biological behaviors of osteoblasts through receptors.The direct effect of Leptin may relate the expression of RANKL/OPG.Then,the balance between bone resorption and bone formation was broken and finally osteoporosis occurred.Lepin may promote osteogenesis and inhibit bone resorption in the differentiation of BMSCs.

Objective:To investigate the relation between eotaxin, STAT6 and allergic rhinitis through comparing expressions of eotaxin and STAT6 in nasal mucosa of normal guinea pigs and of guinea pig allergic rhinitis models. Methods: Twenty-four healthy guinea pigs were equally randomized into 2 groups: normal control and allergic rhinitis models. Guinea pig allergic rhinitis models were established by the ovalbumin challenge method. Protein expressions of eotaxin and STAT6 in the nasal mucosa of the 2 groups were detected by immunohistochemical technique. Results: Expressions of eotaxin and STAT6 were found in the nasal mucosa of both normal guinea pigs and allergic rhinitis guinea pigs. Eotaxin was mostly located in the cytoplasm of the serous and mucous glands,while STAT6 mostly in the cytoplasm of subepithelial infiltrative cells.Expressions of eotaxin and STAT6 in the nasal mucosa of allergic rhinitis guinea pigs were significantly higher than those of normal guinea pigs~(P