Abnormal methylation of the tumor-related genes is frequently found in the development of gastric cancer.Cancer cells exhibit two opposing methylation abnormalities:genome-wide hypomethylation and certain tumor suppressor gene hypermethylation.Furthermore,it seems that the level of DNA methylation is closely associated with the prognosis of gastric cancer.Recently,a great deal of research has been conducted to reveal the corelation of DNA methylation and gastric cancer.

Objective: To probe into essence of syndrome types of blood stasis syndrome. Methods: High shearing whole blood viscosity (?HB). low shearing whole blood viscosity (?LB), plasma viscosity (?P), aggregation index of erythrocyte (AI), hematocrit (HCT) and levels of serum total cholesterol (CH), triglyceride (TG), plasma fibrinogen (Fb), prostacyclin (PGI2), thromboxane (TXA2) were determined in 216 cases of coronary heart disease with blood stasis syndrome. Results: ?LB and AI increased and PGI2/TXA2 ratio decreased significantly in the group of stagnation of Qi and stasis of blood, showing high aggregation state of blood: CH, HCT, Fb, AI and ?LB increased significantly in the group of blood stasis due to Yin -deficiency, showing high concentration, high agglutination and high aggregation state of blood : ?LB, ?P and TG increased significantly in the group of stagnation of phlegm and blood, showing high viscosity state of blood; only ?HB had some increase in the group of Qi - deficiency and blood stasis . Conclusion: Different syndrome types of blood stasis syndromehave different biochemical material base, and changes of blood physical and chemical properties are closely related with TCM syndrome types, which provides an experimental basis for treatment of subtypes of blood stasis syndrome.

OBJECTIVE: To summarize the clinical datas of thepatients with invasive laryngeal fungal infections in, discuss pathogenesis and treatment methods. METHOD: Eleven cases of invasive laryngeal fmycosis who were collected from September 2006 to February 2010 with electronic laryngoscopy, aspirate smear and culture and tissue biopsy for pathological diagnosis, were restrospectively analyzed. Those patients were received iv fluconazole, treatment of Oxygen Atomization of amphotericin B solution and taking itraconazole orally. The hepatic and renal functions of the patients were monitored in the course of treatment. RESULT: All the cases were diagnosed of invasive laryngeal mycosis. 1 patient showed liver dysfunction in the second week during treatment. And continuing the treatment after using liver protection drugs. All symptoms of the patients were improved and no recurrence happened during the 1-6 years of follow-up. CONCLUSION Invasive laryngeal fmycosis was correlated with occupation exposure, abusing of antibiotics and low immunity. Laryngeal mycosis was Diagnosised mainly depended on the pathological examination. The positive rates of the secretion smear was low. The effects of iv fluconazole, Oxygen Atomization of amphotericin B 2-4 weeks, and 4 weeks of taking itraconazole orally were safety and reliable.
Administration, Oral ; Amphotericin B ; therapeutic use ; Antifungal Agents ; therapeutic use ; Chemical and Drug Induced Liver Injury ; prevention & control ; Fluconazole ; therapeutic use ; Humans ; Itraconazole ; therapeutic use ; Laryngeal Diseases ; drug therapy ; etiology ; pathology ; Mycoses ; drug therapy ; etiology ; pathology

BACKGROUND:Interbody fusion is widely used in spine surgeries. Failed fusion may cause many complications, so it is very important to evaluate fusion before surgery. Imaging methods are commonly used to evaluate fusion, including X ray, CT and MRI. Above methods have their own specific methods and characteristics, which cause their differences in sensibility and specificity. OBJECTIVE:To review the research progress of imaging methods in interbody fusion. METHODS:The English key words were“lambar fusion, cervical fusion, interbody fusion, evaluation method”in the PubMed database. The literatures with detailed description of interbody fusion were selected for further review, including comparative study on a variety of evaluation methods of imaging means. RESULTS AND CONCLUSION:According to the inclusion criteria and exclusion criteria, a total of 40 papers were included. X-ray and CT are the most commonly used methods, while CT is more reliable. MRI, bone scan and other methods cannot correctly and directly evaluate spinal fusion. Overal judgment of symptoms, signs and different kinds of image data are more reasonable fusion evaluation strategies.

Objective To explore the operation method and curative effect of small incision minimally invasivepectus excavatum with non thoracoscopic xiphoid.Methods Clinical data of 12 cases with small incision minimally invasive pectus excavatum non thoracoscopic subxiphoid were retrospectively analyzed The index operation time,operation of the surgical bleeding,postoperative complications,postoperative hospitalization time and curative effect evaluation etc,were analyzed.Results 12 patients were successfully completed surgery.The time of operation was (35 ± 8)min;intraoperative blood loss was 5-20 mL,average 10mL;1 patients had pneumothorax postoperative complications;postoperative hospitalization time 3-7day,average 5days;postoperative effect evaluation were excellent in 12 cases.Conclusion Non thoracoscope assisted small incision under xiphoid minimally invasive pectus excavatum surgery is minimally invasive,beautiful appearance,reliable effect and other advantages,and can simplify the operation steps for primary hospital.

Objective: To evaluate the efficacy of the 45S5 bioactive glass on the reduction of hypersensitivity of teeth prepared for full coverage crowns.Methods: In the study, 31 prepared abutment teeth with vital pulp from 18 patients satisfied the inclusion criteria and exclusion criteria.The patients were informed consent and recruited into the study.This was a before and after self-control, single blind clinical trial.The severity of tooth hypersensitivity at baseline was evaluated, after application of the placebo, which was the powder of zinc oxide, and after application of the 45S5 bioactive glass on the same tooth.Tooth hypersensitivity was evaluated by Yeaple probe and visual analogue scale (VAS) when the tooth was stimulated by a fixed value of mechanical pressure and a blast of cold air respectively.And the dentist gave the subjective score, and the Schiff score at the same time.The three methods of measurement were repeated at baseline, after application of placebo and after application of the powder of 45S5 bioactive glass.Student''s t test and nonparametric test were used for statistical analysis.Results: The indexes measured at baseline, after the application of the placebo and after the application of the 45S5 bioactive glass were as follows, respectively: Tactile value (29.03±9.44) g, (29.68±9.48) g and (44.19±11.19) g, VAS value (50.79±22.92) mm, (46.63±22.06) mm and (30.90±20.30) mm, Schiff score 2.13±0.67, 1.97±0.66 and 1.42±0.56.After the application of bioactive glass, the values of Tactile, the VAS, and the Schiff score were statistically different from those at baseline or after application of the placebo (P<0.05).The data measured at baseline and after application of the placebo were of no statistical difference (P>0.05).Statistical differences between the Schiff scores of the groups were associated with gender, tooth position, primary or secondary tooth preparation.Conclusion: Results of the clinical trial showed that the powders of 45S5 bioactive glass were significantly effective on reducing the hypersensitivity of teeth with vital pulp after the full crown preparation.

ObjectiveTo investigate the preventive effect of drugs imitating the ischemic preconditio ning to the donor lung.MethodsAdenosine was used to imitate the early protection of ischemic preconditioning, SOD to imitate the delayed protection and both of them to imitate the integral p rocess of ischemic preconditioning. At the same time, a control group was set up . The rabbits' lungs were flushed, the heart-lung blocs harvested, and adenosin e, SOD, both or none of them wadded respectively. After preservation for 6 h, th e lungs were reperfused for 1 h with the isolated rabbit lung reperfusion model. The PaO 2, airway pressure, pulmonary compliance, wet/dry ratio and content of NOS were measured. The microscopic and electron microscopic examinations were c arried out.ResultsPaO 2 values were the highest in the control group, followed by the SOD group, the adenosine group, the combined use group. The differences among the groups we re significant. The wet/dry ratio was the highest in the control group, followed by the adenosine group, the SOD group, the combined use group with the differen ces among the groups being significant. The airway pressure and NOS content in t he imitating ischemic preconditioning groups was lower than that of the control group. The pulmonary compliance of the imitating ischemic preconditioning groups was higher than that of the control group. However, the differences were not si gnificant. The microscopic and electron microscopic examination revealed that th e pathological changes in the imitating ischemic preconditioning groups was obvi ously lightened as compared with those in the control group.ConclusionsAdministration of the drugs imitating ischemic preconditioning could improve pul monary oxygenation function and ameliorate pulmonary edema. Although the drugs s howed less effect in ameliorating airway pressure, pulmonary compliance and NOS activity, the imitating ischemic preconditioning could protect the donor lung.

Objective To understand the distribution of the blood donors with positive blood test results in Wuzhou .Methods The detection results of ALT ,HBsAg ,anti‐HCV ,anti‐HIV and anti‐TP of 108 879 cases of blood donors were retrospectively ana‐lyzed in 2010 -2012 .Results In the 108 879 cases of blood donors ,the total positive rate of 5 indicators was 6 .01% (6 548/108 879) .The positive rates of ALT ,HBsAg ,anti‐HCV ,anti‐HIV and anti‐TP were 4 .86% ,0 .57% ,0 .20% ,0 .07% and 0 .59% , respectively .The positive rates of ALT ,HBsAg and anti‐HCV were significantly different between males and females (P<0 .05) . The blood donors in 18-30 age group had the highest positive rates of ALT and anti‐TP ,and the blood donors in >30 -40 age group had the highest anti‐HCV positive rate .Conclusion Enhancing the blood donation propaganda ,doing a good job in the blood before consulting ,and choosing low risk blood donors are important to guarantee of the blood quality and reduction of blood transfu‐sion transmitted diseases .

Objective To study the change of neurohormonal factors during CABG and find the effective perioperative treatments for coronary artery disease patients with high-risks. Methods Forty-eight patients were divided into on-pump CABG group(group A, n=23) and off-pump CABG group(group B, n=25). The clinical results were observed and blood samples were collected at six time-points. Angiotensin II(AG II), aldosterone(ALD), atrial natriuretic peptide (ANP), insulin, glucose and cTnI levels in plasma were measured respectively. Results The AG II levels in plasma increased in both groups and reached the peak at 6 h after operation in group A and 3 h after operation in group B, respectively. The peak level of ALD in plasma were at 6 h in group A, and 0 h in group B. The level of ANP in plasma was highest at 0 h in group A, and at the midoperation in group B. The insulin level in plasma was highest at 24 h in group A and at 6 h in group B. The insulin level at 24 h in group A was significantly higher than that in group B. The glucose level reached the peak at 3 h after operation in group A and at 0 h after operation in group B. The levels of AG II, ALD, ANP and glucose in plasma in group A were significantly higher than those in group B at any time-point. The cTnI levels were highest at 24 h in both groups. The cTnI levels in group A were significantly higher than group B at time-points. Conclusion CABG can produce hyperosmotic urinative effects and hyperaldosteronism, which in CABG group is strong and persistent effects due to the hyperosmotic urinative effects and hyperaldosteronism. In OPCAB group is light and short urinative effects mainly due to the hyperosmotic. ACEI, ARB and aldosterone antagonists should be used during the perioperation of CABG. During the early postoperative period, the plasma glucose should be well controlled and GIK solution with magnesium is needed.

Objective To investigate the relationship between the distribution of abdominal adipose tissue and metabolic abnormality in the patients with metabolic syndrome (MS). Methods 108 patients (MS 70 cases, essential hypertension (EH) 22 cases and type Ⅱ diabetes mellitus (T2DM) 16 cases) were enrolled in this study. The visceral adipose (VA) and subcutaneous adipose (SA) were measured with computerized tomography (CT), the ratio of VA/SA was calculated and the correlation between them was analyzed. Results (1) The VA value in MS group was significantly higher compared to both T2DM and EH groups (P