The effect of hyperestrogenemia induced by intramuscular injectied estradiol (E_2) on myocardial contractility and relaxation was observed in female rats with myocardial infarction (MI). The results showed that: (1) In MI group, the plasma estradiol level increased slyghtly and then droped, while the level of estradiol increased markedly in rats treated by E_2 in MI (MIE_2) group. (2) Diameter of myofibril in MIE_2 grows more than that of control sham operation(CS) and MI (10.65?0.59 VS 7.65?0.40 and 10.15?0.54?m, P

Objective To explore the method of repairing foot soft tissue defects by using island skin flaps pedicled with sural nerves and vessels. Methods 24 patients with foot soft tissue defects were treated surgically. The defects were repaired with island skin flaps pedicled with ipsilateral sural nerves and vessels. Results Of all the 24 flaps, 21 flaps survived at the first intention. Partial necrosis appeared in 3 flaps but were cured at the second intention. The follow ups of 1 to 2 years showed that the appearance and function were satisfactory in all the cases except 4 in which the skin appeared swollen. Conclusion To repair foot soft tissue defects, this flap has the advantages of strong anti infection ability and good texture so that healing at the first intention and early functional recovery can be obtained.

Artificial ureter has been studied for nearly 60 years, however it is still in experimental stage. So far there are no commercially available products for clinical use except autologous intestines loop even though many progresses have been made. Each approach for making artificial ureter has its own inherent advantages, disadvantages and limitations. But some approaches have shown great potential to be used as ureteral substitutes. Preventing the conduit from salt depositing and ensuring a watertight anastomoses with native tissues are the key factors in designing and manufacturing the prosthetic ureter. Along with scientific and technical improvement, artificial ureter will be successfully used in clinical therapeuticsin in the very near future.

Objective To determine the clinical value of intraoperative cholangiography in mini-cholecystectomy. Methods The clinical data of 506 patients receiving intraoperative cholangiography in minicholecystectomy from 1992 to 2006 in our hospital were analyzed retrospectively. Results Intr-aoperative cholangiography was successfully completed in 403 patients. The success rate was 97.4%.Stones in the bile duct in 49 patients,abnormal bite duet in 4 patients and bile duct injury in 4 patient were found. The cuts were extended in 16 patients. Conclusion Intraoperative cholangiography is of great value in inducing postoperative complications and improving the quality of MC. Therefore, it can be used to prevent residual stone after operation, avoid bile duct exploration or injury, decrease the chance of extending the cut and make sure of abnormal bile duct.

Objective To observe the clinical effect and safety of propranololand imiquimod therapy for infantile hemangiomas of proliferative phase.Methods A total of 53 infantile patients with hemangiomas of proliferative phase that were treated in our department were collected.According to patient's condition,the patients were classified into treatment group and observation group.The treatment group consisted of 34 patients received propranolol and imiquimod treatment,and 19 cases belonged to the observation group.The changes of tumor were compared between the treatment group and the observation group.Pre-therapeutic and post-therapeutic heart rate,blood pressure,blood glucose were monitored in the treatment group,so were in post-treatment.Results After 6 months,the effective rate was 97.1％ (33/34) in treatment group,and the effective rate was 57.9％ (11/19)in observation group.There was significant difference between treatment group and observation group in effective rate (P＜0.05),and so did blood pressure and heart rate after medication in the treatment group,but not in blood glucose,heart rate six hours after medication (P＞0.05).Conclusions Propranolol and imiquimod therapy has a certain inhibitory effect on infantile hemangiomas of proliferative phase.Propranolol and imiquimod are safe in patients with infantile hemangioma,and adverse reactions are mild.

Objective To understand the efficacy of meibomian gland compression by themselves and outpatient meibomian gland dredge treatment with dry eye young patients of meibomian gland dysfunction.Methods Selected 48 young patients with dry eye of meibomian gland dysfunction,patients were divided into two groups according to the wishes,28 patients were given method guidance of meibomian gland compression by themselves (compression group),every 10 minutes,three times a day,4 weeks after the referral.Other 20 cases meibomian gland dredge by a doctor at the eye clinic performed under anesthesia (dredge group),2 weeks,4 weeks after the referral and then dredge,3 times.All patients were given to health education,all patients before treatment and after treatment for 4 weeks underwent Schirmer Ⅰ test (S Ⅰ t),tear film breakup time (BUT),corneal fluorescein staining (CFL) examination and compared.Results The level of BUT,S Ⅰ t,CFL before treatment in two groups had no significant difference (P ＞ 0.05).After treatment for 4 weeks,eye symptom had improved in two groups.The level of BUT,CFL before and after treatment for 4 weeks in two groups had significant difference (P ＜ 0.05).But the level of S Ⅰ t,BUT,CFL after treatment for 4 weeks between two groups had no significant difference (P ＞ 0.05).Conclusion Dry eye young patients of meibomian gland dysfunction if they can correctly perform meibomian gland compression by themselves can also be reached effectively alleviate dry eye and ocular discomfort symptom improvement purposes.

Objective To investigate the effects of emulsified isoflurane preconditioning on lung ischemia-reperfusion (Ⅰ/R) injury in rats.Methods Thirty-two male Sprague-Dawley rats,weighing 250-320 g,were randomly divided into 4 groups (n =8 each) using a random number table:sham operation group (group S),group Ⅰ/R,intralipid group (group IL),and emulsified isoflurane preconditioning group (group EI).Lung Ⅰ/R was produced by clamping the left hilum of lungs for 1 h followed by 2 h of reperfusion in anesthetized rats.In group S,intraperitoneal normal saline 10.5 ml/kg was injected,but the hilum was only exposed 24 h later.In groups Ⅰ/R,IL and EI,intraperitoneal normal saline 10.5 ml/kg,30％ intralipid 10.5 ml/kg and 8％ emulsified isoflurane 10.5 ml/kg were injected intraperitoneally,respectively,and then the model was established 24 h later.At the end of reperfusion,blood samples were collected from the left ventricle for measurement of PaO2,PaCO2,plasma malondialdehyde (MDA) concentration (by ELISA).The rats were then sacrificed,and left lungs were removed immediately for microscopic examination and for determination of myleoperoxidase (MPO) activity (using colorimetric method) in pulmonary specimens.Wet/dry lung weight ratio (W/D ratio) was calculated.Results Compared with group S,W/D ratio,MPO activity,plasma MDA concentration and PaCO2 were significantly increased,and PaO2 was decreased in the other three groups.Compared with group Ⅰ/R,W/D ratio,MPO activity,plasma MDA concentration and PaCO2 were significantly decreased,and PaO2 was increased in IL and EI groups.W/D ratio,MPO activity,plasma MDA concentration and PaCO2 were significantly lower,and PaO2 was higher in group EI than in group IL.The pathological changes of the lung were significantly attenuated in group EI compared with group Ⅰ/R.Conclusion Emulsified isoflurane preconditioning can reduce lung Ⅰ/R injury in rats,and inhibition of pulmonary inflammatory responses and of production of oxygen free radicals are involved in the mechanism.

The idea and methods of study on proteomics have many similarities to the whole view and the dialectical view of TCM,so it confirms with own characteristics of syndromes to introduce proteomics into studies of essence of syndromes.Appling the idea and methods of proteomics to study TCM differentiation of syndromes of hepatic disease and medicines for treatment and prevention of hepatic disease will comprehensively reveal the essence of generation,development and transformation of TCM hepatic disease,providing the most essential theoretical basis for TCM prevention and treatment of hepatic disease.To establish transform mode concept of correlation of proteomics with TCM hepatic disease will enrich greatly theory of TCM treatment of hepatic disease based on differentiation of syndromes,and also will provide a new thinking model for study on TCM syndrome types,and accelerate the process of TCM modernization.

Objective To investigate a safe, highly efficient and rapid skin expansion method. Methods Syringe pump and pump control of automatic (PCA) replacemented normal affusion were used with shorter and vertical skin professional jargon for continuous and constant pressure skin expansion. Twenty cases (23 expanders) were carried out in this way. Results The maximum area of defect was 16 cm?20 cm, and the minimum area of defect was 3 cm?3 cm. The expanders were used for 18 days and the volume of expanders were 40-900 ml . It was more rapidly than normal affusion. There were no more complications except a case of expander leakage, and others expressed their satifactions Conclusion This modification of the skin expansion is a safe, comfortable, convenient to carry with. It is worth recommendation.