Objective To explore the protective effect mechanism of minocyline on the model of the cerebral ischemia-reperfusion of gerbils by decreasing expression of phosphorylation P38MAPK.Methods A cerebral ischemia-reperfusion model in gerbils was established by clamping both common carotids.The ultrastructural alteration in pyramidal neuron in the hippocampal CA1 and the cortex of frontal lobe at different time was observed by electron microscope.And observe the expression level of phosphorylation P38MAPK by using immunohistochemistry technique.Results ①The damage to the neunos became more and more severe as gerbils survived longer postocclusion.Minocyline treated groups were lessened obviously.② Treatment with minocycline reduced expression of phosphorylation P38MAPK with a significant difference(P

Objective To discuss the clinical effect and the application value of total mesentery excision to the rectal cancerous patients. Methods The clinical data of 67 rectal cancerous patients who received the radical resection which following the TME principle,and with a 2 ～3 years follow-up were analyzed. Results All the 67 patients received the radical resection,including 1 anastomotic fistula,3 anastomotic stenosis, neither local recurrence nor distant metastasis. Conclusion TME played an important role in preventing the local recurrence of rectal cancer, and it met the standards of surgical treatment of rectal cancer better.

winter. The dominant species of algae were Microcystis aeruginosa, Anabaena spiroides and Spirulina . The annual average contents of total nitrogen, total phosphorus and chlorophyl-a were 4. 30 mg/ L, 0.09 mg/ L and 0.015 mg/ L respectively, all of which exceeded the related temporary standards in China. Conclusion The source water of Chaohu Lake had been in the state of serious eutrophication.

Objective To explore the toxicity of algae in water of Chaohu Lake and its influence on the quality of drinking water.Methods In1999,the water samples were collected from the sampling location of A point as a discarded water source for Chaohu Water Plant 1.5km far from the lakeside of Chaohu Lake and the sampling location of B point as a water source in use for Chaohu Water Plant 3.0km far from the lakeside of Chaohu Lake.The species and count of algae in these water samples were analyzed.The determination of micro cystin,sanitary indexes of water quality,Ames test,micronucleus test and SCGE test were carried out in the water samples from B point and the finished water from water plants using water source for B point.Re -sults83species of algae belong to8phylum and49genus were found in water samples from A point and B point in Chao hu Lake.The anual averages of algae count in water sample of B point (8205.67?10 4 /L)exceeded16.8%of that of A point water samples(7024.27?10 4 /L).Microcystin was found in12water samples among total16water samples collected from the lake water,two of which revealed excessive content s of microcystin exceeding standard,but it was not found in raw water and finished water samples from five plants in Hefei and Chaohu Lake[except the4th water plant (4th WP )].After transportation and stor-age,the raw water of4th WP of Hefei with lower content s of microcystin revealed higher contents of microcystin exceeding the standard in primary finished water samples of clean-well finished water storage,but it couldnt be found in secondary finished water samples with the processes of coagulation,ch lorination and active carbon-filtration.The total nitrogen and other6indexes of raw water of4th WP exceeded the related standards ruled by GB3838-2002Surface Water Environment Standard Grade V in different degree,but above6water quality indexes could reach the related sanitary standard after water treatment.Ames test and micronucleus test indicated that the raw water of4th WP presented suspectable mutagenicities which couldnt be removed by co-agulation and chlorination.SCEG test also showed that the organic extracts of the raw water of4th WP presented DNA damage effects.Con clusion The water source in use in Chaohu Lake revealed heavier pollution by algae compared with the original discarded water source in Chaohu Lake.Chaohu Lake water and its finished water revealed higher levels of microcystin exceed-ing the standard and the organic extracts from raw water and finished water presented suspectable mutagenicity.

Objective To estimate the effects of occupational noise exposure to menstrual function of female cotton spinner. Methods 214 female workers of Anqing Textile Factory and urban community were investigated using questionnaire and the data of newly-married dairy by prospective cohort study from 1996 to 2000.The level of noise in their working place was measured. Results (1) The average level of occupational noise exposure of female workes of Anqing Textile Factory and urban community were 98.1 dB and 58.1 dB respectively. (2) Menstrual cycle of women in higher noise exposure group significantly shortened compared with those of the control group (P

[Objective]To evaluate the possibility and clinical value of total en bloc spondylectomy for the treatment of spinal tumors. [Method]Four cases of primary tumor of the thoracolumbar spine were treated with total en bloc spondylectomy TES through a single posterior approach performed in three cases(T10 solitary plasmacytoma,T8 hemangioma,and T12 hemangioma),and one case through a single stage anterior and posterior combined approach(L4 neuroblastoma with paraspinal mass).Improvements in osteotomy apparatus and surgical technique of TES were investigated.[Result]All patients attained significant clinical improvement after surgery without any severe complications.Patients were observed for 13 to 22 months.There was no local recurrence and distal metastasis by the last follow-up.[Conclusion]Total en bloc spondylectomy includes resection of the involved vertebra in two major blocs,rather than a piecemeal pattern.It is one of the most effective therapies for spinal tumors.Howerer,some components of TES remain to be further improved.

Objective To investigate the ultrastructural features and it's significance of the skip area with giant cell arteritis(GCA).Methods 20 GCA patients and 7 non-GCA patients underwent temporal artery biopsies. The sections were stained with HE and methylene blue(MB) for light microscopy(LM), doubly stained with uranyl acetate and lead curate for electron microscopy (EM). 20 GCA patients were divided into skip area GCA group(14 cases) and active inflammatory cell infiltration GCA group(6 cases) according to the definition of skip area or skip lesion. The ultrastructural changes of each group were observed by EM and assessed by using 4 grades from 0 to 3 scores.Results The scores of ultrastructural changes were as follows: the skip area GCA group, 0 in 1 patient, 2 to 6 in 9 patients, and 8 to 9 in 4 patients; the active inflammatory cell infiltration GCA group, 7 to 15 in 6 patients; the non-GCA group, 0 to 1 in 6 patients, and 5 in 1 patient. The scores of the three groups were tested by H check, the difference was evidently significant( P0.05) in comparison with active inflammatory cell infiltration GCA group.Conclusions At electron microscopic level, the skip area of temporal artery biopsy with GCA showed obvious pathological changes, especially in the intima of artery. The results suggest that the investigation of ultrastructure features of the skip areas of temporal artery with GCA may be benefit for the diagnosis and treatment of this disease.

Objective To investigate the effect of high glucose on proliferation of rat mesangial cells and reactive oxygen species (ROS) in culture and the intervention of polysaccharides-2b from Mudan Cortex (PSM2b). Methods Cell proliferation was assessed by MTT colorimetric assay. Colorimetric assay was used to detect SOD and MDA in the supernatant of high glucose culture of rat mesangial cells. Results PSM2b could significantly inhibit the proliferation of mesangial cells, the inhibiting effect showed a dose-dependent manner. And SOD increased with PSM2b compared with that only with high glucose, while MDA decreased. Conclusion High glucose contributes to an increase in rat mesangial cells and ROS produced by rat mesangial cells and PSM2b can better control high glucose-induced proliferation of rat mesangial cells and oxidative stress.

Objective To investigate the effects of different degrees and duration of hypothermia on severe traumatic brain injury in dogs. Methods Thirty-six one-year-old healthy dogs weighing 13-15 kg were used in this study. Traumatic brain injury model was estabhshed according to the method described by Feeney. Orthogonal design was used in this experiment. Two empirical factors: temperature (factor A) and duration (factor B) were used. Body temperature was maintained at 38 ℃ (A1), 31 ℃ (A2) or35 ℃ (A3) for 6 h (B1) or 12 h (B2).The dogs were divided into 6 groups: group A1B1 , A1B2, A2B1, A2B2, A3B1 and A3B2. Blood gas analysis was performed after the target temperature was maintained for the target duration. Neurological deficit was assessed and scored (0 = no deficit, 500 = severe neurological deficit) and blood samples were obtained at 24, 48 and 72 h after brain injury for determination of serum concentrations of neuron-specific enolase (NSE) and myelin basic protein (MBP) and plasma S-100β protein concentration. Brains were removed at the 72 h after brain injury for determination of Bcl-2 and Bax expression and detection of apoptosis in the brain. Results Hypothermia significantly decreased serum NSE, MBP and plasma S-100β concentrations, neuronal apoptosis and NDS scores;up-regulated Bcl-2 expression and down-regulated Bax expression in brain tissue in the 4 hypothermia groups (group A2B1, A2B2, A3B1 and A3B2) as compared with the control groups (group A1 B1, A1 B2). Best neuroprotective effects were observed in group A3 B1 (35 ℃ for 6 h) in terms of serum NSE, NBP and plasma S-100β concentrations, neuronal apoptosis and cerebral Bax and Bcl-2 expression, but there was no significant difference in the NDS scores among the 4 hypothermia groups. Conclusion Hypothermia can provide neuroprotection in a dog model of traumatic brain injury but the neuroprotective effect is independent of the degree and duration of hypothermia.