Objective To investigate the effect of preconditioning with different doses of emulsified isoflurane on focal cerebral ischemia-reperfusion (I/R) injury in rats. Methods Ninety-six male SD rats were randomly divided into 6 groups ( n = 16 each): sham operation group (group S), I/R group, low, median and high doses of emulsified isoflurane preconditioning group (group L, M, H) and intralipid group (group IL). Middle cerebral artery occlusion was produced by inserting a nylon thread. In group S, intraperitoneal normal saline (NS)10.5 ml/kg was injected, but the artery was only exposed 24 h later. In group I/R, intraperitoneal NS 10.5 ml/kg was injected, and the model was established 24 h later. In group L, M, H and IL, 8% emulsified isoflurane 3.5 ml/kg+NS 7.0 ml/kg, 8% emulsified isoflurane 7.0 ml/kg + NS 3.5 ml/kg, 8% emulsified isoflurane 10.5 ml/kg and 30% intralipid 10.5 ml/kg were injected intraperitoneally respectively, and then the model was established 24 h later. The temperature, HR and RR were recorded at 10 min before ischemia and at 10 min of reperfusion. The neurological deficit was scored, the cerebral infarct volume and apoptosis in neurons were detected, and microscopic examination of ischemic penumbra region was performed at 24 h of reperfusion. Results The rectal temperature and HR were significantly increased, while RR was significantly decreased duing cerebral I/R.The neurological deficit score was significantly higher, cerebral infarct volume and the number of apoptotic neurons were significantly larger in the other groups than in group S. Emulsified isoflurane preconditioning reduced neurological deficit scores, cerebral infarct volume and the number of apoptotic neurons dose-dependently. Conclusion Emulsified isoflurane preconditioning can reduce focal cerebral I/R injury in a dose-dependent manner in rats.

Objective To investigate the effect of postconditioning with 8 % emulsified isoflurane (EI) on focal cerebral ischemia-reperfusion (I/R) injury in rats. Methods Forty-eight male SD rats weighing 260-300 g were randomly divided into 6 groups ( n = 8 each): group Ⅰ sham operation (group S); group Ⅱ I/R; group Ⅲ,Ⅳ, Ⅴ 3 different doses of EI (group L-EI, M-EI, H-.EI) and group Ⅵ lipid emulsion (group LE). Right middle cerebral artery occlusion (MCAO) was induced by inserting a nylon thread 0.24 mm in diameter into right internal carotid artery. The thread was threaded cranially until resistance was met. MCAO was maintained for 2 h followed by 24 h reperfusion in group Ⅱ-Ⅵ. In group Ⅲ-Ⅴ EI 3.5, 7.0 and 10.5 ml/kg were injected intraperitoneally (IP) immediately before reperfusion respectively. In group LE 30% lipid emulsion 10.5 ml/kg was given instead of EI. The neurologic deficit was assessed and scored (0 = normal, 4 = unable to move and unconscious). The animals were then killed and infarct size was measured. Results IP 8% emulsified isoflurane 7.0 and 10.5 ml/kg injected before reperfusion significantly reduced neurologic deficit scores and infarct size in group M-EI and H-EI as compared with group I/R. Conclusion Postconditioning with 8% emulsified isoflurane can protect the brain against focal cerebral I/R injury.

Objective To investigate the effect of preconditioning with emulsified isoflurane (eISO) on neuronal apoptosis in hippocampal CA1 region induced by focal cerebral ischemia-reperfusion (I/R) injury in rats. Methods Forty-eight healthy adult male SD rats weighing 250-300 g were randomly divided into 6 groups (n = 8 each): sham operation group (group S); I/R group; eISO + I/R group (group EI); LY294002 (a specific PI3K inhibitor) + eISO + I/R group (group L+ EI); LY294002 + I/R group (group L) and DMSO (solvent for LY294002) + I/R group (group DMSO). Focal cerebral I/R was induced by 2 h middle cerebral artery occlusion ( MCAO). A nylon thread (0.26 mm in diameter) with rounded tip was inserted into internal carotid artery and advanced cranially until resistance was met (depth of insertion about 18-20 mm) . eISO 10.5 ml/kg (120 mg/ml) was injected intraperitoneally (IP) in groups EI and L+ EI. LY294002 (25 mmol/L) 5 pi was injected into cerebral ventricle on the ischemic side in group L + EI ( at 30 min before eISO) and group L. DMSO 5 μl was injected into the cerebral ventricle on ischemic side before MCAO in group DMSO. Neurologic deficit was assessed and scored (0 = normal, 4 = unconscious) at 24 h of reperfusion. The animals were then killed and their brains were removed for detection of neuronal apoptosis (by TUNEL) and p-Akt expression (by immuno-histochemistry) in hippocampal CA1 region. Results Cerebral I/R significantly increased the neurologic deficit scores, the number of apoptotic cells and p-Akt expression in group I/R as compared with group S. Preconditioning with elSO attenuated the I/R-induced increase in neurologic deficit scores and number of apoptotic cells but further increased p-Akt expression. The neuroprotective effect of eISO preconditioning against I/R-induced changes was counteracted by LY294002. Conclusion eISO preconditioning can attenuate focal cerebral I/R-induced neuronal apoptosis in rats by activating PI3K/Akt pathway.

AIM: To study the effect of integrin ?2 on adhesion of SK-N-SH neuroblastoma cells to collagen. METHODS: Adhesion of the SK-N-SH cells to immobilized collagen was tested with various concentration of Mg~ 2+ , Ca~ 2+ and with 10 ?g/L anti-?2 monoclonal antibody (mAb) 6F1. A_ 570 was detected as adhesion cell numbers. RESULTS: Mg~ 2+ -dependent adhesion of SK-N-SH cells to type I collagen was increased significantly, with peak adhesion at concentration of 1 mmol/L Mg~ 2+ . A_ 570 with or without Mg~ 2+ was 0.59?0.03 and 0.25?0.01 respectively (P

Objective:To discuss the mechanism of Baoganning anti-liver fibrosis.Methods:liver fibrosis rat models were established by complex factors.Automatic biochemical analyzer was used to detect TC、TG and RIA isotope analysis was used to detect serum leptin.To observe the influence of Baoganning on TC、TG and leptin level of liver fibrosis model rats. Results:Compared with the normal group,levels of serum TC,TG decreased apparently in model and every drug groups(P0.05).Conclusion:Baoganning can effectively prevent and treat liver fibrosis in rats,which may be closely related to the improvement of liver function,adjustment of lipid and reduction of serum leptin.

Objective To explore the anti-hepatic-fibrosis mechanism of Baogan Ning(BN).Methods Fifty Wistar rats were randomized into 5 groups: normal group,model group,high-and low-dosage BN groups(35.4 and 17.7 g/kg respectively),and colchicine group(0.11mg/kg).Rat models of hepatic fibrosis were induced by multiplex factors.Hepatic protein was extracted,and the expression of hepatic leptic receptors of OB-Rb,JAK2 and STAT3 was detected with Western Blot method.Results The protein expression was not obvious in the normal group,and the color of protein band in the medication groups was light but dark in the model group.Compared with the normal group,the expression of OB-Rb,JAK2 and STAT3 increased in the model group.However,the expression of OB-Rb,JAK2 and STAT3 decreased in the medication groups as compared with the model group.And the expression of OB-Rb,JAK2 and STAT3 decreased in the BN groups as compared with the colchicines group.Conclusion The anti-hepatic-fibrosis mechanism of Baogan Ning is probably related with the inhibition of OB-Rb expression,thus inhibit the JAK2/STAT3 message pathway.

Objective To evaluate the efficacy and safety ofGuipi Decoction vs. antidepressants for depression. Methods Articles about the randomized controlled trials (RCTs) ofGuipi Decoction for depression were searched in the databases of CNKI, VIP, Wanfang Data, CMB, Embase, PubMed, and Cochrane Library. Two researchers independently screened the articles, extracted the data, and evaluated the methodological quality. Meta-analyses were performed by using RevMan 5.2 software.Results A total of 9 RCTs articles involving 893 cases were included. Meta-analysis results suggested that as compared with antidepressants only,Guipi Decoction together with antidepressants was associated with a significantly greater reduction in HAMD scores (MD=-4.63, 95%CI [-5.95, -3.32];P<0.001). There was no significant difference in HAMD scores betweenGuipi Decoction used alone and antidepressants (MD=-0.30, 95%CI [-4.22, 3.62];P=0.88). As compared with antidepressants,Guipi Decoction together with antidepressants increased in the rates of total effective events (RR=1.26, 95%CI [1.14, 1.38];P<0.001). No serious adverse reactions were reported. Meta-analysis results suggested that the incidence of adverse reactions of Guipi Decoction(RR=0.48, 95%CI [0.40, 0.58];P<0.001) andGuipi Decoction together with antidepressants (RR=0.51, 95%CI [0.30, 0.85];P=0.010) were lower than that of antidepressants alone.Conclusion The present evidence suggests thatGuipi Decoction is effective and relatively safe for patients with depression. However, because the quality of included research was low, more large-scale RCTs with multicenter and high quality are needed for further verification.

Objective To know the focus groups' iodine nutrition level after implementation of a new standard of salt iodization,and to provide technical support for iodine deficiency disorders (IDD) prevention in Nanping City.Methods According to the requirements of Focus Groups' Iodine Nutrition and Related Health Monitoring Program in Fujian Province,Yanping,Jian'ou,Zhenghe,Jianyang and Shaowu counties (cities,districts) were selected for monitoring in 2014;Pucheng,Songxi,Wuyishan,Shunchang and Guangze counties (cities,districts) were selected for monitoring in 2015.We studied:①The water iodine:2 tap water samples were collected from centralized water supply system;from decentralized water supply system,2 residents drinking water samples were collected according to each of five directions of east,west,south,north,and center,for determination of water iodine content.②Salt iodine:4 administrative villages (neighborhood committees) were selected from each monitoring site,from which 15 households were selected,edible salt samples were collected,and salt iodine content was tested.③Urinary iodine:one primary school was selected from each monitoring site,30-40 students aged from 8-10 years old were selected from each primary school,urine samples were collected for detecting urinary iodine.④Salt intake per capita:among the 8-10 years old students whom had been collected urine samples,10 students' family edible salt samples were collected,then the salt intake per capita was measured via 3-days weighing method.⑤ Goiter:in Jianyang and Shaowu Cities in 2014,and in Guangze and Shunchang Cities in 2015,the thyroid volume was measured using ultrasound,and goiter rate was calculated of 8-10 years old students whom had been collected urine samples before.⑥Pregnant women urinary iodine:20 pregnant women (early,middle and late pregnancy were included) were selected in each monitoring site,urine samples were collected for detecting urinary iodine.Results Investigation results in 2014 and 2015 were as follows:61 and 50 samples of daily drinking water of the two years were investigated,the medians of drinking water iodine were 1.5 and 5.7 μg/L for each group of samples,respectively;and daily salt intake of 253 and 250 residents of the two years were investigated,and the medians intake were 6.8 and 6.9 g/d,respectively;1 500 and 1 500 residential edible salt samples in the two years were tested,iodized salt coverage rates were 99.7％ (1 496/1 500) and 99.9％ (1 498/1 500),respectively,qualified rates of iodized salt were 95.7％ (1 431/1 496) and 94.5％ (1 416/1 498),respectively,qualified iodized salt consumption rates were 95.4％ (1 431/1 500) and 94.4％ (1 416/ 1 500),respectively;of which 749 and 1 000 8-10 years old children's urinary iodine were also tested,and children's urinary iodine medians were 190.1 and 210.4 μg/L,respectively;300 and 275 thyroids of children aged from 8 to 10 were tested,and goiter rates (ultrasound method) were 1.3％ (4/300) and 2.9％ (8/275),respectively;500 and 500 pregnant women urinary iodine were tested in the two years,and pregnant women urinary iodine medians were 133.2 and 150.3 μg/L,respectively,the pregnant women urinary iodine median of the Nanping City of 10 counties (cities,districts) and 7 counties (cities,districts) were less than 150 μg/L.Conclusions Nanping City's natural environment iodine is deficient.After the new standard of salt iodization,we can continue to promote the process of the standard of eliminating IDD in China,besides,8-10 years old children's iodine nutrition is basically at the appropriate level,but the level of pregnant women has showed a slightly deficiency.

Objective To investigate the prevalence of BRCA1/2 gene mutations among Uygur and Han sporadic breast cancer patients in Xinjiang Uygur Automous.Methods Polymerase chain reaction ( PCR) and DNA se-quencing was used to detect mutations of BRCA1(exons 2, 11(11A and 11B) and 20) and BRCA2(exon 11) genes in the Paraffin imbedding tissues from 230 sporadic breast cancer patients ( 115 Uygur and 115 Han ) in Xinjiang Uygur Automous.Results In the 230 cases of sporadic breast cancer patients, 16 cases have gene mu-tation ( 16/230 ,6.96%) .One case of BRCA1 gene in 16 cases of mutations -5 382 locus mutation and 7 cases of new mutations.There was 2 germline mutation in exon 11 of BRCA2 gene.BRCA gene mutation rates of Uygur and Han patients were 7.83% ( 9/115 ) and 6.09% ( 7/115 ) .The onset age of mutations group were 50 or less.Mutations group of patients with amenorrhea ( 3 ) were less than whom were premenopausal ( 13 ) ( P <0.05 ) .Conclusions The prevalence of BRCA1 mutations was significantly higher than BRCA2 in sporadic breast cancer patients of Xinjiang.

ObjectiveTo establish an animal model of implanted inferior vena cava tumor thrombus (IVCTT)and examine its growth with MDCT and 3D-MPR. MethodsTumor cell line VX2 was inoculated subcutaneously into rabbit to develop the primary tumor, which was then cut into small strips. Purse-string suture was performed on the anterior wall of IVC after the laparotomy in eighteen New Zealand white rabbits.The tumor strip was injected into IVC through the purse and suspensory fixed on the inner wall of IVC. The general conditions,body weight,and the survival time were monitored after operations.MDCT examinations were performed with plain scan,arterial phase,portal phase and venous phase enhancement every week for all animals and 3D-MPR were acquired.The volumes of IVCTT were calculated.IVC,IVCTT and metastasis were examined with gross and histological pathology. ResultsThe IVCTT was confirmed by MDCT and 3D-MPR images.Collateral varicose veins caused by IVC obstruction and metastasis were also shown in images.IVCTT and metastasis were confirmed by pathological method. The success rate of IVCTT was 100 ％. The mean survival time of operated animals was(49.5±4.4)days. ConclusionsInjecting and suspensory fixing VX2 tumor strip into IVC is a reliable method to establish the IVCTT animal model. MDCT and 3D-MPR are valuable methods to monitor the growth and metastasis of IVCTT in animal models. The model of implanted IVCTT of rabbits provides a useful tool for the research of treatment of IVCTT.