Pinacidil caused a concentration -dependent decrease in the force of contraction,but, benzyltetrahydropalmatine ( BTHP ) increased the contractile force in isolated guinea pig left atria. Both BTHP and pinacidil suppressed the adrenaline- induced left atria auto-maticity.In isolated guinea pig trachealis,BTHP(5, 50 ?mol ?L-1) caused an apparent leftward shift of acetylcholine(Ach)or histamine dose-response curves, augmenting response to low Ach or histamine concentrations without or slightly change the tissue maximum response to the spasmogen. By contraries,Pinacidil(50 ? mol?L-1)produced a rightward shifts in the concentration - effect curve of Ach or Kis and depressed the foot of thedose-response curves for Ach or His without modifying the maximum response.In the presence of pinacidil BTHP did not modify the responses to either Ach or histamine. indeed,the specific antagonism between pinacidil and BTHP suggests that BTHP interact with the same or closely related sites of actions as the K channel blocker.

Objective To investigate clinic characteristics and prognosis of lacunar cerebra infarction.Methods 112 patients with lacunar cerebra infarction were selected and analyzed retrospectively.Results 40% of patients had slight symptom,symptom-free 25% of patients was observed by the infarct sign based on the CT brain scan or MRI,65% of patients was the frequency of cerebra infarction,infarct focus was 219 in the 112 cases,recrudescence in 76 patients including 35 patients(46%) with symptom of dementia,language barrier and diplegia.Conclusions Clinic symptom of patients with lacunar cerebra infarction is slight,whereas clinic characteristics are variegated.Recrudescence rate is highter in patients with a previous history of the lacunarr cerebra infraction,and prognosis is bad.With the number of infarct focus increasing,clinic symptom of cognize obstacle and other function abnormity become more serious.This results may help clinicians to assess prognosis more accurately.

The effect of hyperestrogenemia induced by intramuscular injectied estradiol (E_2) on myocardial contractility and relaxation was observed in female rats with myocardial infarction (MI). The results showed that: (1) In MI group, the plasma estradiol level increased slyghtly and then droped, while the level of estradiol increased markedly in rats treated by E_2 in MI (MIE_2) group. (2) Diameter of myofibril in MIE_2 grows more than that of control sham operation(CS) and MI (10.65?0.59 VS 7.65?0.40 and 10.15?0.54?m, P

Objective To determine the clinical value of intraoperative cholangiography in mini-cholecystectomy. Methods The clinical data of 506 patients receiving intraoperative cholangiography in minicholecystectomy from 1992 to 2006 in our hospital were analyzed retrospectively. Results Intr-aoperative cholangiography was successfully completed in 403 patients. The success rate was 97.4%.Stones in the bile duct in 49 patients,abnormal bite duet in 4 patients and bile duct injury in 4 patient were found. The cuts were extended in 16 patients. Conclusion Intraoperative cholangiography is of great value in inducing postoperative complications and improving the quality of MC. Therefore, it can be used to prevent residual stone after operation, avoid bile duct exploration or injury, decrease the chance of extending the cut and make sure of abnormal bile duct.

Objective:To study the biological characteristics and mineralization activity of strom cells (MSC) isolated from rat bone marrow.Methods: MSCs were isolated from rat bone marrow using gradient centrifuge (460 g,20 min) with 70% percoll and cultured with DMEM. The cells were then incubated with mineralization culture medium (MCM) in the presence of Dex (10～7 mol/L), β-GP (10 mmol/L) and AcA (50 g/L).Cell growth was studied with cell counting,alkaline phosphatase (ALP) expression with a kit and mineralization with a saturated solution of alizarin red S (pH 4.3) stainning.Results: The isolated cells from bone marrow of rat were epithelial-like. The doubling time (DT) of the cells in MCM or DMEM were 50.3 h and 66.1 h respectively. Mineralization was observed after the cells had been cultured in MCM for 28 days. bFGF and rhBMP-2 increased ALP expression of MSC in a dosage dependent manner. Conclusion:In vitro cultured MSCs may be induced to differentiate towards osteoblasts.

砄bjective:To study the biological characteristics and mineralization activity of strom cells (MSC) isolated from rat bone marrow.Methods: MSCs were isolated from rat bone marrow using gradient centrifuge (460 g,20 min) with 70% percoll and cultured with DMEM. The cells were then incubated with mineralization culture medium (MCM) in the presence of Dex (10～7 mol/L), ? GP (10 mmol/L) and AcA (50 g/L).Cell growth was studied with cell counting,alkaline phosphatase (ALP) expression with a kit and mineralization with a saturated solution of alizarin red S (pH 4.3) stainning.Results: The isolated cells from bone marrow of rat were epithelial like. The doubling time (DT) of the cells in MCM or DMEM were 50.3 h and 66.1 h respectively. Mineralization was observed after the cells had been cultured in MCM for 28 days. bFGF and rhBMP 2 increased ALP expression of MSC in a dosage dependent manner. Conclusion: In vitro cultured MSCs may be induced to differentiate towards osteoblasts.

Objective This paper reviewed the researching progress on chronic renal failure(CRF)of traditional Chinese medicine in the recent ten years,hoping to provide theoretical evidence for both clinical treatment and scientific study.Methods Literatures on CRF researched by traditional medicine in the recent ten years were summarized and analyzed.Results At present,the studies on CRF by traditional medicine focus on its etiological factors,pathogenesis and treatment according to syndrome differentiation,which revealed the mechanism of its curative effect on different levels.Conclusions Traditional medicine has good curative effect on CRF,and it is worthy of being developed and investigated.

Objective To summarize the application of ruyi jinhuang powder dissolved in ethanol in the treatment of chemotherapeutic phlebitis induced by indwelling needle. Methods 88 cases of patients with chemotherapeutic phlebitis were randomly divided into the observation group and the control group with 44 cases in each group. The observation group was given wet compress using ruyi jinhuang powder dissolved in ethanol, while the control group used hot towels of 40 to 50℃. The treatment effect was compared between the two groups. Results The treatment effect of the observation group was better than that of the control group. Conclusions Treatment of chemotherapeutic phlebitis using wet compress with tracditional Chinese medicine showed significant effect, which is an effective method in the treatment of chemotherapeutic phlebitis induced by indwelling needle.